Screening and identification of severe acute respiratory syndrome-associated coronavirus-specific CTL epitopes.
Identifieur interne : 002011 ( PubMed/Checkpoint ); précédent : 002010; suivant : 002012Screening and identification of severe acute respiratory syndrome-associated coronavirus-specific CTL epitopes.
Auteurs : Minghai Zhou ; Dongping Xu ; Xiaojuan Li ; Hongtao Li ; Ming Shan ; Jiaren Tang ; Min Wang ; Fu-Sheng Wang ; Xiaodong Zhu ; Hua Tao ; Wei He ; Po Tien ; George F. GaoSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 2006.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (immunologie), Agranulocytes (immunologie), Agranulocytes (virologie), Animaux, Antigène HLA-A2, Antigènes HLA-A (biosynthèse), Antigènes HLA-A (métabolisme), Cellules cultivées, Déterminants antigéniques des lymphocytes T (immunologie), Déterminants antigéniques des lymphocytes T (isolement et purification), Déterminants antigéniques des lymphocytes T (métabolisme), Fragments peptidiques (administration et posologie), Fragments peptidiques (immunologie), Fragments peptidiques (isolement et purification), Fragments peptidiques (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (administration et posologie), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (isolement et purification), Glycoprotéines membranaires (métabolisme), Humains, Interféron gamma (métabolisme), Liaison aux protéines (immunologie), Liquide intracellulaire (immunologie), Liquide intracellulaire (métabolisme), Liquide intracellulaire (virologie), Lymphocytes T cytotoxiques (immunologie), Lymphocytes T cytotoxiques (métabolisme), Lymphocytes T cytotoxiques (virologie), Protéines de l'enveloppe virale (administration et posologie), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (isolement et purification), Protéines de l'enveloppe virale (métabolisme), Protéines nucléocapside (immunologie), Protéines nucléocapside (isolement et purification), Protéines nucléocapside (métabolisme), Souris, Souris transgéniques, Test ELISA, Vaccins à ADN (administration et posologie), Vaccins à ADN (immunologie), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification), Virus du SRAS (métabolisme).
- MESH :
- administration et posologie : Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins à ADN.
- biosynthèse : Antigènes HLA-A.
- immunologie : Activation des lymphocytes, Agranulocytes, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Liaison aux protéines, Liquide intracellulaire, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- isolement et purification : Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- métabolisme : Antigènes HLA-A, Déterminants antigéniques des lymphocytes T, Fragments peptidiques, Glycoprotéines membranaires, Interféron gamma, Liquide intracellulaire, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- virologie : Agranulocytes, Liquide intracellulaire, Lymphocytes T cytotoxiques.
- Animaux, Antigène HLA-A2, Cellules cultivées, Glycoprotéine de spicule des coronavirus, Humains, Souris, Souris transgéniques, Test ELISA.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Epitopes, T-Lymphocyte (immunology), Epitopes, T-Lymphocyte (isolation & purification), Epitopes, T-Lymphocyte (metabolism), HLA-A Antigens (biosynthesis), HLA-A Antigens (metabolism), HLA-A2 Antigen, Humans, Interferon-gamma (metabolism), Intracellular Fluid (immunology), Intracellular Fluid (metabolism), Intracellular Fluid (virology), Leukocytes, Mononuclear (immunology), Leukocytes, Mononuclear (virology), Lymphocyte Activation (immunology), Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (immunology), Membrane Glycoproteins (isolation & purification), Membrane Glycoproteins (metabolism), Mice, Mice, Transgenic, Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (isolation & purification), Nucleocapsid Proteins (metabolism), Peptide Fragments (administration & dosage), Peptide Fragments (immunology), Peptide Fragments (isolation & purification), Peptide Fragments (metabolism), Protein Binding (immunology), SARS Virus (immunology), SARS Virus (isolation & purification), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), T-Lymphocytes, Cytotoxic (metabolism), T-Lymphocytes, Cytotoxic (virology), Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (immunology), Viral Envelope Proteins (isolation & purification), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Peptide Fragments, Vaccines, DNA, Viral Envelope Proteins.
- chemical , biosynthesis : HLA-A Antigens.
- chemical , immunology : Epitopes, T-Lymphocyte, Membrane Glycoproteins, Nucleocapsid Proteins, Peptide Fragments, Vaccines, DNA, Viral Envelope Proteins.
- chemical , isolation & purification : Epitopes, T-Lymphocyte, Membrane Glycoproteins, Nucleocapsid Proteins, Peptide Fragments, Viral Envelope Proteins.
- chemical , metabolism : Epitopes, T-Lymphocyte, HLA-A Antigens, Interferon-gamma, Membrane Glycoproteins, Nucleocapsid Proteins, Peptide Fragments, Viral Envelope Proteins.
- immunology : Intracellular Fluid, Leukocytes, Mononuclear, Lymphocyte Activation, Protein Binding, SARS Virus, T-Lymphocytes, Cytotoxic.
- isolation & purification : SARS Virus.
- metabolism : Intracellular Fluid, SARS Virus, T-Lymphocytes, Cytotoxic.
- virology : Intracellular Fluid, Leukocytes, Mononuclear, T-Lymphocytes, Cytotoxic.
- Animals, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, HLA-A2 Antigen, Humans, Mice, Mice, Transgenic, Spike Glycoprotein, Coronavirus.
Abstract
Severe acute respiratory syndrome (SARS) is a highly contagious and life-threatening disease that emerged in China in November 2002. A novel SARS-associated coronavirus was identified as its principal etiologic agent; however, the immunopathogenesis of SARS and the role of special CTLs in virus clearance are still largely uncharacterized. In this study, potential HLA-A*0201-restricted spike (S) and nucleocapsid protein-derived peptides were selected from an online database and screened for potential CTL epitopes by in vitro refolding and T2 cell-stabilization assays. The antigenicity of nine peptides which could refold with HLA-A*0201 molecules was assessed with an IFN-gamma ELISPOT assay to determine the capacity to stimulate CTLs from PBMCs of HLA-A2(+) SARS-recovered donors. A novel HLA-A*0201-restricted decameric epitope P15 (S411-420, KLPDDFMGCV) derived from the S protein was identified and found to localize within the angiotensin-converting enzyme 2 receptor-binding region of the S1 domain. P15 could significantly enhance the expression of HLA-A*0201 molecules on the T2 cell surface, stimulate IFN-gamma-producing CTLs from the PBMCs of former SARS patients, and induce specific CTLs from P15-immunized HLA-A2.1 transgenic mice in vivo. Furthermore, significant P15-specific CTLs were induced from HLA-A2.1-transgenic mice immunized by a DNA vaccine encoding the S protein; suggesting that P15 was a naturally processed epitope. Thus, P15 may be a novel SARS-associated coronavirus-specific CTL epitope and a potential target for characterization of virus control mechanisms and evaluation of candidate SARS vaccines.
DOI: 10.4049/jimmunol.177.4.2138
PubMed: 16887973
Affiliations:
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pubmed:16887973Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Screening and identification of severe acute respiratory syndrome-associated coronavirus-specific CTL epitopes.</title><author><name sortKey="Zhou, Minghai" sort="Zhou, Minghai" uniqKey="Zhou M" first="Minghai" last="Zhou">Minghai Zhou</name><affiliation><nlm:affiliation>Center for Molecular Immunology and Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, The People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">The People's Republic of China</wicri:noCountry></affiliation></author><author><name sortKey="Xu, Dongping" sort="Xu, Dongping" uniqKey="Xu D" first="Dongping" last="Xu">Dongping Xu</name></author><author><name sortKey="Li, Xiaojuan" sort="Li, Xiaojuan" uniqKey="Li X" first="Xiaojuan" last="Li">Xiaojuan Li</name></author><author><name sortKey="Li, Hongtao" sort="Li, Hongtao" uniqKey="Li H" first="Hongtao" last="Li">Hongtao Li</name></author><author><name sortKey="Shan, Ming" sort="Shan, Ming" uniqKey="Shan M" first="Ming" last="Shan">Ming Shan</name></author><author><name sortKey="Tang, Jiaren" sort="Tang, Jiaren" uniqKey="Tang J" first="Jiaren" last="Tang">Jiaren Tang</name></author><author><name sortKey="Wang, Min" sort="Wang, Min" uniqKey="Wang M" first="Min" last="Wang">Min Wang</name></author><author><name sortKey="Wang, Fu Sheng" sort="Wang, Fu Sheng" uniqKey="Wang F" first="Fu-Sheng" last="Wang">Fu-Sheng Wang</name></author><author><name sortKey="Zhu, Xiaodong" sort="Zhu, Xiaodong" uniqKey="Zhu X" first="Xiaodong" last="Zhu">Xiaodong Zhu</name></author><author><name sortKey="Tao, Hua" sort="Tao, Hua" uniqKey="Tao H" first="Hua" last="Tao">Hua Tao</name></author><author><name sortKey="He, Wei" sort="He, Wei" uniqKey="He W" first="Wei" last="He">Wei He</name></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. 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Gao</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Epitopes, T-Lymphocyte (isolation & purification)</term><term>Epitopes, T-Lymphocyte (metabolism)</term><term>HLA-A Antigens (biosynthesis)</term><term>HLA-A Antigens (metabolism)</term><term>HLA-A2 Antigen</term><term>Humans</term><term>Interferon-gamma (metabolism)</term><term>Intracellular Fluid (immunology)</term><term>Intracellular Fluid (metabolism)</term><term>Intracellular Fluid (virology)</term><term>Leukocytes, Mononuclear (immunology)</term><term>Leukocytes, Mononuclear (virology)</term><term>Lymphocyte Activation (immunology)</term><term>Membrane Glycoproteins (administration & dosage)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (isolation & purification)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Mice, Transgenic</term><term>Nucleocapsid Proteins (immunology)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Peptide Fragments (administration & dosage)</term><term>Peptide Fragments (immunology)</term><term>Peptide Fragments (isolation & purification)</term><term>Peptide Fragments (metabolism)</term><term>Protein Binding (immunology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>T-Lymphocytes, Cytotoxic (metabolism)</term><term>T-Lymphocytes, Cytotoxic (virology)</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (administration & dosage)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes (immunologie)</term><term>Agranulocytes (immunologie)</term><term>Agranulocytes (virologie)</term><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A (biosynthèse)</term><term>Antigènes HLA-A (métabolisme)</term><term>Cellules cultivées</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Déterminants antigéniques des lymphocytes T (isolement et purification)</term><term>Déterminants antigéniques des lymphocytes T (métabolisme)</term><term>Fragments peptidiques (administration et posologie)</term><term>Fragments peptidiques (immunologie)</term><term>Fragments peptidiques (isolement et purification)</term><term>Fragments peptidiques (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (administration et posologie)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (isolement et purification)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Interféron gamma (métabolisme)</term><term>Liaison aux protéines (immunologie)</term><term>Liquide intracellulaire (immunologie)</term><term>Liquide intracellulaire (métabolisme)</term><term>Liquide intracellulaire (virologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Lymphocytes T cytotoxiques (métabolisme)</term><term>Lymphocytes T cytotoxiques (virologie)</term><term>Protéines de l'enveloppe virale (administration et posologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (isolement et purification)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (isolement et purification)</term><term>Protéines nucléocapside (métabolisme)</term><term>Souris</term><term>Souris transgéniques</term><term>Test ELISA</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>HLA-A Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Peptide Fragments</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>HLA-A Antigens</term><term>Interferon-gamma</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes HLA-A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Activation des lymphocytes</term><term>Agranulocytes</term><term>Déterminants antigéniques des lymphocytes T</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Liaison aux protéines</term><term>Liquide intracellulaire</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Intracellular Fluid</term><term>Leukocytes, Mononuclear</term><term>Lymphocyte Activation</term><term>Protein Binding</term><term>SARS Virus</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes T</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Intracellular Fluid</term><term>SARS Virus</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes HLA-A</term><term>Déterminants antigéniques des lymphocytes T</term><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Interféron gamma</term><term>Liquide intracellulaire</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Agranulocytes</term><term>Liquide intracellulaire</term><term>Lymphocytes T cytotoxiques</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Intracellular Fluid</term><term>Leukocytes, Mononuclear</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Enzyme-Linked Immunosorbent Assay</term><term>HLA-A2 Antigen</term><term>Humans</term><term>Mice</term><term>Mice, Transgenic</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Cellules cultivées</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Souris</term><term>Souris transgéniques</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a highly contagious and life-threatening disease that emerged in China in November 2002. A novel SARS-associated coronavirus was identified as its principal etiologic agent; however, the immunopathogenesis of SARS and the role of special CTLs in virus clearance are still largely uncharacterized. In this study, potential HLA-A*0201-restricted spike (S) and nucleocapsid protein-derived peptides were selected from an online database and screened for potential CTL epitopes by in vitro refolding and T2 cell-stabilization assays. The antigenicity of nine peptides which could refold with HLA-A*0201 molecules was assessed with an IFN-gamma ELISPOT assay to determine the capacity to stimulate CTLs from PBMCs of HLA-A2(+) SARS-recovered donors. A novel HLA-A*0201-restricted decameric epitope P15 (S411-420, KLPDDFMGCV) derived from the S protein was identified and found to localize within the angiotensin-converting enzyme 2 receptor-binding region of the S1 domain. P15 could significantly enhance the expression of HLA-A*0201 molecules on the T2 cell surface, stimulate IFN-gamma-producing CTLs from the PBMCs of former SARS patients, and induce specific CTLs from P15-immunized HLA-A2.1 transgenic mice in vivo. Furthermore, significant P15-specific CTLs were induced from HLA-A2.1-transgenic mice immunized by a DNA vaccine encoding the S protein; suggesting that P15 was a naturally processed epitope. Thus, P15 may be a novel SARS-associated coronavirus-specific CTL epitope and a potential target for characterization of virus control mechanisms and evaluation of candidate SARS vaccines.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16887973</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1767</ISSN><JournalIssue CitedMedium="Print"><Volume>177</Volume><Issue>4</Issue><PubDate><Year>2006</Year><Month>Aug</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of immunology (Baltimore, Md. : 1950)</Title><ISOAbbreviation>J. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Screening and identification of severe acute respiratory syndrome-associated coronavirus-specific CTL epitopes.</ArticleTitle><Pagination><MedlinePgn>2138-45</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a highly contagious and life-threatening disease that emerged in China in November 2002. A novel SARS-associated coronavirus was identified as its principal etiologic agent; however, the immunopathogenesis of SARS and the role of special CTLs in virus clearance are still largely uncharacterized. In this study, potential HLA-A*0201-restricted spike (S) and nucleocapsid protein-derived peptides were selected from an online database and screened for potential CTL epitopes by in vitro refolding and T2 cell-stabilization assays. The antigenicity of nine peptides which could refold with HLA-A*0201 molecules was assessed with an IFN-gamma ELISPOT assay to determine the capacity to stimulate CTLs from PBMCs of HLA-A2(+) SARS-recovered donors. A novel HLA-A*0201-restricted decameric epitope P15 (S411-420, KLPDDFMGCV) derived from the S protein was identified and found to localize within the angiotensin-converting enzyme 2 receptor-binding region of the S1 domain. P15 could significantly enhance the expression of HLA-A*0201 molecules on the T2 cell surface, stimulate IFN-gamma-producing CTLs from the PBMCs of former SARS patients, and induce specific CTLs from P15-immunized HLA-A2.1 transgenic mice in vivo. Furthermore, significant P15-specific CTLs were induced from HLA-A2.1-transgenic mice immunized by a DNA vaccine encoding the S protein; suggesting that P15 was a naturally processed epitope. Thus, P15 may be a novel SARS-associated coronavirus-specific CTL epitope and a potential target for characterization of virus control mechanisms and evaluation of candidate SARS vaccines.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Minghai</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Center for Molecular Immunology and Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, The People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Dongping</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiaojuan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hongtao</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Shan</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Jiaren</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Min</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Fu-Sheng</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Xiaodong</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Hua</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Tien</LastName><ForeName>Po</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>George F</ForeName><Initials>GF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Immunol</MedlineTA><NlmUniqueID>2985117R</NlmUniqueID><ISSNLinking>0022-1767</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018984">Epitopes, T-Lymphocyte</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015234">HLA-A Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C435939">HLA-A*02:01 antigen</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015789">HLA-A2 Antigen</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical><Chemical><RegistryNumber>82115-62-6</RegistryNumber><NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018984" MajorTopicYN="N">Epitopes, T-Lymphocyte</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015234" MajorTopicYN="N">HLA-A Antigens</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015789" MajorTopicYN="N">HLA-A2 Antigen</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007424" MajorTopicYN="N">Intracellular Fluid</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007963" MajorTopicYN="N">Leukocytes, Mononuclear</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008822" MajorTopicYN="N">Mice, Transgenic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013602" MajorTopicYN="N">T-Lymphocytes, Cytotoxic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>8</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16887973</ArticleId><ArticleId IdType="pii">177/4/2138</ArticleId><ArticleId IdType="doi">10.4049/jimmunol.177.4.2138</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name><name sortKey="He, Wei" sort="He, Wei" uniqKey="He W" first="Wei" last="He">Wei He</name><name sortKey="Li, Hongtao" sort="Li, Hongtao" uniqKey="Li H" first="Hongtao" last="Li">Hongtao Li</name><name sortKey="Li, Xiaojuan" sort="Li, Xiaojuan" uniqKey="Li X" first="Xiaojuan" last="Li">Xiaojuan Li</name><name sortKey="Shan, Ming" sort="Shan, Ming" uniqKey="Shan M" first="Ming" last="Shan">Ming Shan</name><name sortKey="Tang, Jiaren" sort="Tang, Jiaren" uniqKey="Tang J" first="Jiaren" last="Tang">Jiaren Tang</name><name sortKey="Tao, Hua" sort="Tao, Hua" uniqKey="Tao H" first="Hua" last="Tao">Hua Tao</name><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><name sortKey="Wang, Fu Sheng" sort="Wang, Fu Sheng" uniqKey="Wang F" first="Fu-Sheng" last="Wang">Fu-Sheng Wang</name><name sortKey="Wang, Min" sort="Wang, Min" uniqKey="Wang M" first="Min" last="Wang">Min Wang</name><name sortKey="Xu, Dongping" sort="Xu, Dongping" uniqKey="Xu D" first="Dongping" last="Xu">Dongping Xu</name><name sortKey="Zhou, Minghai" sort="Zhou, Minghai" uniqKey="Zhou M" first="Minghai" last="Zhou">Minghai Zhou</name><name sortKey="Zhu, Xiaodong" sort="Zhu, Xiaodong" uniqKey="Zhu X" first="Xiaodong" last="Zhu">Xiaodong Zhu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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