Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.
Identifieur interne : 001F94 ( PubMed/Checkpoint ); précédent : 001F93; suivant : 001F95Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.
Auteurs : Wei Lu [République populaire de Chine] ; Bo-Jian Zheng ; Ke Xu ; Wolfgang Schwarz ; Lanying Du ; Charlotte K L. Wong ; Jiadong Chen ; Shuming Duan ; Vincent Deubel ; Bing SunSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Cadres ouverts de lecture, Canaux ioniques (génétique), Canaux ioniques (métabolisme), Humains, Lignée cellulaire, Ovocytes (cytologie), Ovocytes (physiologie), Petit ARN interférent (génétique), Petit ARN interférent (métabolisme), Potassium (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), Réplication virale, Techniques de patch-clamp, Virus du SRAS (génétique), Virus du SRAS (métabolisme), Xenopus laevis.
- MESH :
- cytologie : Ovocytes.
- génétique : Canaux ioniques, Petit ARN interférent, Protéines recombinantes, Protéines virales, Virus du SRAS.
- métabolisme : Canaux ioniques, Petit ARN interférent, Potassium, Protéines recombinantes, Protéines virales, Virus du SRAS.
- physiologie : Ovocytes.
- Animaux, Cadres ouverts de lecture, Humains, Lignée cellulaire, Réplication virale, Techniques de patch-clamp, Xenopus laevis.
English descriptors
- KwdEn :
- Animals, Cell Line, Humans, Ion Channels (genetics), Ion Channels (metabolism), Oocytes (cytology), Oocytes (physiology), Open Reading Frames, Patch-Clamp Techniques, Potassium (metabolism), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Viral Proteins (genetics), Viral Proteins (metabolism), Virus Replication, Xenopus laevis.
- MESH :
- chemical , genetics : Ion Channels, RNA, Small Interfering, Recombinant Proteins, Viral Proteins.
- chemical , metabolism : Ion Channels, Potassium, RNA, Small Interfering, Recombinant Proteins, Viral Proteins.
- cytology : Oocytes.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- physiology : Oocytes.
- Animals, Cell Line, Humans, Open Reading Frames, Patch-Clamp Techniques, Virus Replication, Xenopus laevis.
Abstract
Fourteen ORFs have been identified in the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) genome. ORF 3a of SARS-CoV codes for a recently identified transmembrane protein, but its function remains unknown. In this study we confirmed the 3a protein expression and investigated its localization at the surface of SARS-CoV-infected or 3a-cDNA-transfected cells. Our experiments showed that recombinant 3a protein can form a homotetramer complex through interprotein disulfide bridges in 3a-cDNA-transfected cells, providing a clue to ion channel function. The putative ion channel activity of this protein was assessed in 3a-complement RNA-injected Xenopus oocytes by two-electrode voltage clamp. The results suggest that 3a protein forms a potassium sensitive channel, which can be efficiently inhibited by barium. After FRhK-4 cells were transfected with an siRNA, which is known to suppress 3a expression, followed by infection with SARS-CoV, the released virus was significantly decreased, whereas the replication of the virus in the infected cells was not changed. Our observation suggests that SARS-CoV ORF 3a functions as an ion channel that may promote virus release. This finding will help to explain the highly pathogenic nature of SARS-CoV and to develop new strategies for treatment of SARS infection.
DOI: 10.1073/pnas.0605402103
PubMed: 16894145
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16894145Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.</title><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025</wicri:regionArea><wicri:noRegion>Shanghai 200025</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name></author><author><name sortKey="Schwarz, Wolfgang" sort="Schwarz, Wolfgang" uniqKey="Schwarz W" first="Wolfgang" last="Schwarz">Wolfgang Schwarz</name></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></author><author><name sortKey="Wong, Charlotte K L" sort="Wong, Charlotte K L" uniqKey="Wong C" first="Charlotte K L" last="Wong">Charlotte K L. Wong</name></author><author><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name></author><author><name sortKey="Duan, Shuming" sort="Duan, Shuming" uniqKey="Duan S" first="Shuming" last="Duan">Shuming Duan</name></author><author><name sortKey="Deubel, Vincent" sort="Deubel, Vincent" uniqKey="Deubel V" first="Vincent" last="Deubel">Vincent Deubel</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16894145</idno><idno type="pmid">16894145</idno><idno type="doi">10.1073/pnas.0605402103</idno><idno type="wicri:Area/PubMed/Corpus">002133</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002133</idno><idno type="wicri:Area/PubMed/Curation">002133</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002133</idno><idno type="wicri:Area/PubMed/Checkpoint">001F94</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F94</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.</title><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025</wicri:regionArea><wicri:noRegion>Shanghai 200025</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name></author><author><name sortKey="Schwarz, Wolfgang" sort="Schwarz, Wolfgang" uniqKey="Schwarz W" first="Wolfgang" last="Schwarz">Wolfgang Schwarz</name></author><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></author><author><name sortKey="Wong, Charlotte K L" sort="Wong, Charlotte K L" uniqKey="Wong C" first="Charlotte K L" last="Wong">Charlotte K L. Wong</name></author><author><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name></author><author><name sortKey="Duan, Shuming" sort="Duan, Shuming" uniqKey="Duan S" first="Shuming" last="Duan">Shuming Duan</name></author><author><name sortKey="Deubel, Vincent" sort="Deubel, Vincent" uniqKey="Deubel V" first="Vincent" last="Deubel">Vincent Deubel</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Ion Channels (genetics)</term><term>Ion Channels (metabolism)</term><term>Oocytes (cytology)</term><term>Oocytes (physiology)</term><term>Open Reading Frames</term><term>Patch-Clamp Techniques</term><term>Potassium (metabolism)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Virus Replication</term><term>Xenopus laevis</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Canaux ioniques (génétique)</term><term>Canaux ioniques (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Ovocytes (cytologie)</term><term>Ovocytes (physiologie)</term><term>Petit ARN interférent (génétique)</term><term>Petit ARN interférent (métabolisme)</term><term>Potassium (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Réplication virale</term><term>Techniques de patch-clamp</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Xenopus laevis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Ion Channels</term><term>RNA, Small Interfering</term><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ion Channels</term><term>Potassium</term><term>RNA, Small Interfering</term><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Ovocytes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Oocytes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Canaux ioniques</term><term>Petit ARN interférent</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canaux ioniques</term><term>Petit ARN interférent</term><term>Potassium</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ovocytes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Open Reading Frames</term><term>Patch-Clamp Techniques</term><term>Virus Replication</term><term>Xenopus laevis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Humains</term><term>Lignée cellulaire</term><term>Réplication virale</term><term>Techniques de patch-clamp</term><term>Xenopus laevis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fourteen ORFs have been identified in the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) genome. ORF 3a of SARS-CoV codes for a recently identified transmembrane protein, but its function remains unknown. In this study we confirmed the 3a protein expression and investigated its localization at the surface of SARS-CoV-infected or 3a-cDNA-transfected cells. Our experiments showed that recombinant 3a protein can form a homotetramer complex through interprotein disulfide bridges in 3a-cDNA-transfected cells, providing a clue to ion channel function. The putative ion channel activity of this protein was assessed in 3a-complement RNA-injected Xenopus oocytes by two-electrode voltage clamp. The results suggest that 3a protein forms a potassium sensitive channel, which can be efficiently inhibited by barium. After FRhK-4 cells were transfected with an siRNA, which is known to suppress 3a expression, followed by infection with SARS-CoV, the released virus was significantly decreased, whereas the replication of the virus in the infected cells was not changed. Our observation suggests that SARS-CoV ORF 3a functions as an ion channel that may promote virus release. This finding will help to explain the highly pathogenic nature of SARS-CoV and to develop new strategies for treatment of SARS infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16894145</PMID><DateCompleted><Year>2006</Year><Month>11</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>33</Issue><PubDate><Year>2006</Year><Month>Aug</Month><Day>15</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.</ArticleTitle><Pagination><MedlinePgn>12540-5</MedlinePgn></Pagination><Abstract><AbstractText>Fourteen ORFs have been identified in the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) genome. ORF 3a of SARS-CoV codes for a recently identified transmembrane protein, but its function remains unknown. In this study we confirmed the 3a protein expression and investigated its localization at the surface of SARS-CoV-infected or 3a-cDNA-transfected cells. Our experiments showed that recombinant 3a protein can form a homotetramer complex through interprotein disulfide bridges in 3a-cDNA-transfected cells, providing a clue to ion channel function. The putative ion channel activity of this protein was assessed in 3a-complement RNA-injected Xenopus oocytes by two-electrode voltage clamp. The results suggest that 3a protein forms a potassium sensitive channel, which can be efficiently inhibited by barium. After FRhK-4 cells were transfected with an siRNA, which is known to suppress 3a expression, followed by infection with SARS-CoV, the released virus was significantly decreased, whereas the replication of the virus in the infected cells was not changed. Our observation suggests that SARS-CoV ORF 3a functions as an ion channel that may promote virus release. This finding will help to explain the highly pathogenic nature of SARS-CoV and to develop new strategies for treatment of SARS infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Laboratory of Molecular Virology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Bo-Jian</ForeName><Initials>BJ</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ke</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Schwarz</LastName><ForeName>Wolfgang</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Lanying</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Wong</LastName><ForeName>Charlotte K L</ForeName><Initials>CK</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jiadong</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Shuming</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Deubel</LastName><ForeName>Vincent</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>08</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C487105">3a protein, severe acute respiratory syndrome coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007473">Ion Channels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>RWP5GA015D</RegistryNumber><NameOfSubstance UI="D011188">Potassium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007473" MajorTopicYN="N">Ion Channels</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009865" MajorTopicYN="N">Oocytes</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018408" MajorTopicYN="N">Patch-Clamp Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011188" MajorTopicYN="N">Potassium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014982" MajorTopicYN="N">Xenopus laevis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>11</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>8</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16894145</ArticleId><ArticleId IdType="pii">0605402103</ArticleId><ArticleId IdType="doi">10.1073/pnas.0605402103</ArticleId><ArticleId IdType="pmc">PMC1567914</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Mol Biol. 2004 Jul 30;341(1):271-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15312778</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Mar 23;440(7083):570-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16467789</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1992 May 1;69(3):517-28</Citation><ArticleIdList><ArticleId IdType="pubmed">1374685</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7381-5</Citation><ArticleIdList><ArticleId IdType="pubmed">8346259</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Virol. 1995 Jun;39(3):171-81</Citation><ArticleIdList><ArticleId IdType="pubmed">8579000</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):111-5</Citation><ArticleIdList><ArticleId IdType="pubmed">8552585</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1996 Oct;70(10):7108-15</Citation><ArticleIdList><ArticleId IdType="pubmed">8794357</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1996 Nov 25;398(1):12-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8946945</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 Dec;78(24):14043-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15564512</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Mar;79(5):3182-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15709039</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Signal. 2005 Jul;17(7):869-80</Citation><ArticleIdList><ArticleId IdType="pubmed">15763429</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2005 May;109(2):191-202</Citation><ArticleIdList><ArticleId IdType="pubmed">15763150</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1999 Oct 25;263(2):265-72</Citation><ArticleIdList><ArticleId IdType="pubmed">10544100</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2000 Mar 3;287(5458):1641-4</Citation><ArticleIdList><ArticleId IdType="pubmed">10698737</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2000 May;74(9):4319-26</Citation><ArticleIdList><ArticleId IdType="pubmed">10756047</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2003 Sep 18;552(1):47-53</Citation><ArticleIdList><ArticleId IdType="pubmed">12972151</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2003 Sep 18;552(1):61-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12972153</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2003 Nov 26;290(20):2665-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14645307</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Mar;22(3):326-30</Citation><ArticleIdList><ArticleId IdType="pubmed">14758366</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2004 Apr 23;14(2):259-67</Citation><ArticleIdList><ArticleId IdType="pubmed">15099524</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2004 May 7;565(1-3):111-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15135062</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 Jul;78(13):6723-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15194747</ArticleId></ArticleIdList></Reference><Reference><Citation>Antivir Ther. 2004 Jun;9(3):365-74</Citation><ArticleIdList><ArticleId IdType="pubmed">15259899</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2005 Apr 29;330(1):286-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15781262</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):6160-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15827117</ArticleId></ArticleIdList></Reference><Reference><Citation>Antivir Ther. 2005;10(3):393-403</Citation><ArticleIdList><ArticleId IdType="pubmed">15918330</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2005 Jul;86(Pt 7):1921-30</Citation><ArticleIdList><ArticleId IdType="pubmed">15958670</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Jan;80(1):210-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16352545</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3195-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2014240</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name><name sortKey="Deubel, Vincent" sort="Deubel, Vincent" uniqKey="Deubel V" first="Vincent" last="Deubel">Vincent Deubel</name><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><name sortKey="Duan, Shuming" sort="Duan, Shuming" uniqKey="Duan S" first="Shuming" last="Duan">Shuming Duan</name><name sortKey="Schwarz, Wolfgang" sort="Schwarz, Wolfgang" uniqKey="Schwarz W" first="Wolfgang" last="Schwarz">Wolfgang Schwarz</name><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><name sortKey="Wong, Charlotte K L" sort="Wong, Charlotte K L" uniqKey="Wong C" first="Charlotte K L" last="Wong">Charlotte K L. Wong</name><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F94 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 001F94 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:16894145
|texte= Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:16894145" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |