Human immunodeficiency viral vector pseudotyped with the spike envelope of severe acute respiratory syndrome coronavirus transduces human airway epithelial cells and dendritic cells.
Identifieur interne : 001D90 ( PubMed/Checkpoint ); précédent : 001D89; suivant : 001D91Human immunodeficiency viral vector pseudotyped with the spike envelope of severe acute respiratory syndrome coronavirus transduces human airway epithelial cells and dendritic cells.
Auteurs : Gary P. Kobinger [Canada] ; Maria P. Limberis ; Suri Somanathan ; Gregory Schumer ; Peter Bell ; James M. WilsonSource :
- Human gene therapy [ 1043-0342 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Appareil respiratoire (cytologie), Appareil respiratoire (métabolisme), Cellules cultivées, Cellules dendritiques (métabolisme), Cellules épithéliales (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Humains, Maladies pulmonaires (), Opéron lac, Protéines de l'enveloppe virale (génétique), Souris, Souris de lignée C57BL, Techniques in vitro, Thérapie génétique (), Transduction génétique, VIH (Virus de l'Immunodéficience Humaine) (génétique), Vecteurs génétiques, Virus du SRAS (génétique).
- MESH :
- cytologie : Appareil respiratoire.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, VIH (Virus de l'Immunodéficience Humaine), Virus du SRAS.
- métabolisme : Appareil respiratoire, Cellules dendritiques, Cellules épithéliales.
- Animaux, Cellules cultivées, Glycoprotéine de spicule des coronavirus, Humains, Maladies pulmonaires, Opéron lac, Souris, Souris de lignée C57BL, Techniques in vitro, Thérapie génétique, Transduction génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Dendritic Cells (metabolism), Epithelial Cells (metabolism), Genetic Therapy (methods), Genetic Vectors, HIV (genetics), Humans, In Vitro Techniques, Lac Operon, Lung Diseases (therapy), Membrane Glycoproteins (genetics), Mice, Mice, Inbred C57BL, Respiratory System (cytology), Respiratory System (metabolism), SARS Virus (genetics), Spike Glycoprotein, Coronavirus, Transduction, Genetic, Viral Envelope Proteins (genetics).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- cytology : Respiratory System.
- genetics : HIV, SARS Virus.
- metabolism : Dendritic Cells, Epithelial Cells, Respiratory System.
- methods : Genetic Therapy.
- therapy : Lung Diseases.
- Animals, Cells, Cultured, Genetic Vectors, Humans, In Vitro Techniques, Lac Operon, Mice, Mice, Inbred C57BL, Spike Glycoprotein, Coronavirus, Transduction, Genetic.
Abstract
The human severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly infectious virus that causes severe respiratory infections in humans. The spike envelope glycoprotein of SARS-CoV, the main determinant of SARS-CoV tropism, was isolated and used to pseudotype a human immunodeficiency virus (HIV)-based vector. Spike-pseudotyped HIV vector was generated and evaluated in vitro on well-differentiated human airway epithelial cells and bronchial explants and in vivo in murine airways. The spike envelope was less efficient at promoting HIV vector transduction of murine airway epithelium than an optimized deletion mutant of the Zaire ebolavirus envelope glycoprotein (NTD6L), which was used as a benchmark. However, spike-pseudotyped HIV vector was substantially more efficient than NTD6L-pseudotyped vector on human airway epithelium as demonstrated by lacZ gene transfer in primary cultures of epithelial cells and bronchial explants. In addition, this study shows that spike-pseudotyped HIV -based vector can efficiently transduce human dendritic cells and epithelial cells of the esophagus, which may have implications in investigating mechanisms of SARS-CoV pathogenesis. Spike-pseudotyped HIV-based vector is a novel lung-directed gene transfer vehicle that holds promise for the treatment of genetic lung diseases such as cystic fibrosis or alpha(1)-antitrypsin deficiency.
DOI: 10.1089/hum.2006.194
PubMed: 17518614
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:17518614Le document en format XML
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<author><name sortKey="Kobinger, Gary P" sort="Kobinger, Gary P" uniqKey="Kobinger G" first="Gary P" last="Kobinger">Gary P. Kobinger</name>
<affiliation wicri:level="1"><nlm:affiliation>Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada R3E 3R2.</nlm:affiliation>
<country>Canada</country>
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<author><name sortKey="Limberis, Maria P" sort="Limberis, Maria P" uniqKey="Limberis M" first="Maria P" last="Limberis">Maria P. Limberis</name>
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<author><name sortKey="Bell, Peter" sort="Bell, Peter" uniqKey="Bell P" first="Peter" last="Bell">Peter Bell</name>
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<term>Cells, Cultured</term>
<term>Dendritic Cells (metabolism)</term>
<term>Epithelial Cells (metabolism)</term>
<term>Genetic Therapy (methods)</term>
<term>Genetic Vectors</term>
<term>HIV (genetics)</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Lac Operon</term>
<term>Lung Diseases (therapy)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Respiratory System (cytology)</term>
<term>Respiratory System (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Transduction, Genetic</term>
<term>Viral Envelope Proteins (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Appareil respiratoire (cytologie)</term>
<term>Appareil respiratoire (métabolisme)</term>
<term>Cellules cultivées</term>
<term>Cellules dendritiques (métabolisme)</term>
<term>Cellules épithéliales (métabolisme)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Humains</term>
<term>Maladies pulmonaires ()</term>
<term>Opéron lac</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Techniques in vitro</term>
<term>Thérapie génétique ()</term>
<term>Transduction génétique</term>
<term>VIH (Virus de l'Immunodéficience Humaine) (génétique)</term>
<term>Vecteurs génétiques</term>
<term>Virus du SRAS (génétique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Appareil respiratoire</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Respiratory System</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>VIH (Virus de l'Immunodéficience Humaine)</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Dendritic Cells</term>
<term>Epithelial Cells</term>
<term>Respiratory System</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Therapy</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Appareil respiratoire</term>
<term>Cellules dendritiques</term>
<term>Cellules épithéliales</term>
</keywords>
<keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Lung Diseases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cells, Cultured</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Lac Operon</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Transduction, Genetic</term>
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<term>Cellules cultivées</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Maladies pulmonaires</term>
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<term>Souris</term>
<term>Souris de lignée C57BL</term>
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<front><div type="abstract" xml:lang="en">The human severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly infectious virus that causes severe respiratory infections in humans. The spike envelope glycoprotein of SARS-CoV, the main determinant of SARS-CoV tropism, was isolated and used to pseudotype a human immunodeficiency virus (HIV)-based vector. Spike-pseudotyped HIV vector was generated and evaluated in vitro on well-differentiated human airway epithelial cells and bronchial explants and in vivo in murine airways. The spike envelope was less efficient at promoting HIV vector transduction of murine airway epithelium than an optimized deletion mutant of the Zaire ebolavirus envelope glycoprotein (NTD6L), which was used as a benchmark. However, spike-pseudotyped HIV vector was substantially more efficient than NTD6L-pseudotyped vector on human airway epithelium as demonstrated by lacZ gene transfer in primary cultures of epithelial cells and bronchial explants. In addition, this study shows that spike-pseudotyped HIV -based vector can efficiently transduce human dendritic cells and epithelial cells of the esophagus, which may have implications in investigating mechanisms of SARS-CoV pathogenesis. Spike-pseudotyped HIV-based vector is a novel lung-directed gene transfer vehicle that holds promise for the treatment of genetic lung diseases such as cystic fibrosis or alpha(1)-antitrypsin deficiency.</div>
</front>
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<Title>Human gene therapy</Title>
<ISOAbbreviation>Hum. Gene Ther.</ISOAbbreviation>
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<ArticleTitle>Human immunodeficiency viral vector pseudotyped with the spike envelope of severe acute respiratory syndrome coronavirus transduces human airway epithelial cells and dendritic cells.</ArticleTitle>
<Pagination><MedlinePgn>413-22</MedlinePgn>
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<Abstract><AbstractText>The human severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly infectious virus that causes severe respiratory infections in humans. The spike envelope glycoprotein of SARS-CoV, the main determinant of SARS-CoV tropism, was isolated and used to pseudotype a human immunodeficiency virus (HIV)-based vector. Spike-pseudotyped HIV vector was generated and evaluated in vitro on well-differentiated human airway epithelial cells and bronchial explants and in vivo in murine airways. The spike envelope was less efficient at promoting HIV vector transduction of murine airway epithelium than an optimized deletion mutant of the Zaire ebolavirus envelope glycoprotein (NTD6L), which was used as a benchmark. However, spike-pseudotyped HIV vector was substantially more efficient than NTD6L-pseudotyped vector on human airway epithelium as demonstrated by lacZ gene transfer in primary cultures of epithelial cells and bronchial explants. In addition, this study shows that spike-pseudotyped HIV -based vector can efficiently transduce human dendritic cells and epithelial cells of the esophagus, which may have implications in investigating mechanisms of SARS-CoV pathogenesis. Spike-pseudotyped HIV-based vector is a novel lung-directed gene transfer vehicle that holds promise for the treatment of genetic lung diseases such as cystic fibrosis or alpha(1)-antitrypsin deficiency.</AbstractText>
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<Agency>NHLBI NIH HHS</Agency>
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<Acronym>DK</Acronym>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D014161" MajorTopicYN="Y">Transduction, Genetic</DescriptorName>
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<name sortKey="Wilson, James M" sort="Wilson, James M" uniqKey="Wilson J" first="James M" last="Wilson">James M. Wilson</name>
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