SrasV1, PubMed, Checkpoint, bibRecord, 001C09

A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro.

Identifieur interne : 001C09 ( PubMed/Checkpoint ); précédent : 001C08; suivant : 001C10

A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro.

Auteurs : Xiaohua Wang [République populaire de Chine] ; Wei Xu ; Deyan Tong ; Jing Ni ; Haifeng Gao ; Ying Wang ; Yiwei Chu ; Pingping Li ; Xiaoming Yang ; Sidong Xiong

Source :

Immunology letters [ 0165-2478 ] ; 2008.

RBID : pubmed:18533276

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibody Formation (genetics), Antibody Formation (immunology), Antigens, Viral (chemistry), Antigens, Viral (genetics), Antigens, Viral (immunology), Cloning, Molecular, Epitopes, B-Lymphocyte (chemistry), Epitopes, B-Lymphocyte (genetics), Epitopes, B-Lymphocyte (immunology), Gene Expression (genetics), Gene Expression (immunology), Humans, Immunologic Memory (genetics), Immunologic Memory (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (therapeutic use), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vaccines, DNA (chemistry), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , chemistry : Antigens, Viral, Epitopes, B-Lymphocyte, Vaccines, DNA.
- chemical , genetics : Antigens, Viral, Epitopes, B-Lymphocyte, Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Epitopes, B-Lymphocyte, Membrane Glycoproteins, Recombinant Fusion Proteins, Vaccines, DNA, Viral Envelope Proteins, Viral Matrix Proteins.
- blood : Severe Acute Respiratory Syndrome.
- genetics : Antibody Formation, Gene Expression, Immunologic Memory, SARS Virus, Severe Acute Respiratory Syndrome.
- immunology : Antibody Formation, Gene Expression, Immunologic Memory, SARS Virus, Severe Acute Respiratory Syndrome.
- pathogenicity : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- chemical , therapeutic use : Recombinant Fusion Proteins.
- Animals, Cloning, Molecular, Humans, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.

Abstract

Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S(437-459) and M(1-20). A DNA prime-protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.

DOI: 10.1016/j.imlet.2008.04.005
PubMed: 18533276

Affiliations:

République populaire de Chine

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Le document en format XML

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<term>Membrane Glycoproteins (immunology)</term>
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<term>Recombinant Fusion Proteins (immunology)</term>
<term>Recombinant Fusion Proteins (therapeutic use)</term>
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<term>Mémoire immunologique (immunologie)</term>
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<term>Production d'anticorps (immunologie)</term>
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<front><div type="abstract" xml:lang="en">Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S(437-459) and M(1-20). A DNA prime-protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</div>
</front>
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<Abstract><AbstractText>Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S(437-459) and M(1-20). A DNA prime-protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</AbstractText>
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<Author ValidYN="Y"><LastName>Li</LastName>
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A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro.

A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro.

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