Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2.
Identifieur interne : 001B06 ( PubMed/Checkpoint ); précédent : 001B05; suivant : 001B07Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2.
Auteurs : Alison C. Mathewson [Royaume-Uni] ; Alexandra Bishop [Royaume-Uni] ; Yongxiu Yao [Royaume-Uni] ; Fred Kemp [Royaume-Uni] ; Junyuan Ren [Royaume-Uni] ; Hongying Chen [Royaume-Uni] ; Xiaodong Xu [Royaume-Uni] ; Ben Berkhout [Pays-Bas] ; Lia Van Der Hoek [Pays-Bas] ; Ian M. Jones [Royaume-Uni]Source :
- The Journal of general virology [ 0022-1317 ] ; 2008.
Descripteurs français
- KwdFr :
- Cinétique, Coronavirus (physiologie), Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Indice de gravité médicale, Infections à coronavirus (physiopathologie), Liaison aux protéines, Peptidyl-Dipeptidase A (métabolisme), Protéines de l'enveloppe virale (métabolisme), Solutions, Syndrome respiratoire aigu sévère (physiopathologie), Virus du SRAS (physiologie).
- MESH :
- métabolisme : Glycoprotéines membranaires, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale.
- physiologie : Coronavirus, Virus du SRAS.
- physiopathologie : Infections à coronavirus, Syndrome respiratoire aigu sévère.
- Cinétique, Cytométrie en flux, Glycoprotéine de spicule des coronavirus, Humains, Indice de gravité médicale, Liaison aux protéines, Solutions.
English descriptors
- KwdEn :
- Coronavirus (physiology), Coronavirus Infections (physiopathology), Flow Cytometry, Humans, Kinetics, Membrane Glycoproteins (metabolism), Peptidyl-Dipeptidase A (metabolism), Protein Binding, SARS Virus (physiology), Severe Acute Respiratory Syndrome (physiopathology), Severity of Illness Index, Solutions, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (metabolism).
- MESH :
- chemical , metabolism : Membrane Glycoproteins, Peptidyl-Dipeptidase A, Viral Envelope Proteins.
- physiology : Coronavirus, SARS Virus.
- physiopathology : Coronavirus Infections, Severe Acute Respiratory Syndrome.
- Flow Cytometry, Humans, Kinetics, Protein Binding, Severity of Illness Index, Solutions, Spike Glycoprotein, Coronavirus.
Abstract
Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63.
DOI: 10.1099/vir.0.2008/003962-0
PubMed: 18931070
Affiliations:
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Mathewson</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author><author><name sortKey="Bishop, Alexandra" sort="Bishop, Alexandra" uniqKey="Bishop A" first="Alexandra" last="Bishop">Alexandra Bishop</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author><author><name sortKey="Yao, Yongxiu" sort="Yao, Yongxiu" uniqKey="Yao Y" first="Yongxiu" last="Yao">Yongxiu 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wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Hongying" sort="Chen, Hongying" uniqKey="Chen H" first="Hongying" last="Chen">Hongying Chen</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiaodong" sort="Xu, Xiaodong" uniqKey="Xu X" first="Xiaodong" last="Xu">Xiaodong Xu</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author><author><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam</wicri:regionArea><orgName type="university">Université d'Amsterdam</orgName><placeName><settlement type="city">Amsterdam</settlement><region>Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam</wicri:regionArea><orgName type="university">Université d'Amsterdam</orgName><placeName><settlement type="city">Amsterdam</settlement><region>Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Jones, Ian M" sort="Jones, Ian M" uniqKey="Jones I" first="Ian M" last="Jones">Ian M. Jones</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Reading, Reading RG6 6AJ</wicri:regionArea><wicri:noRegion>Reading RG6 6AJ</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus (physiology)</term><term>Coronavirus Infections (physiopathology)</term><term>Flow Cytometry</term><term>Humans</term><term>Kinetics</term><term>Membrane Glycoproteins (metabolism)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Protein Binding</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (physiopathology)</term><term>Severity of Illness Index</term><term>Solutions</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cinétique</term><term>Coronavirus (physiologie)</term><term>Cytométrie en flux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Indice de gravité médicale</term><term>Infections à coronavirus (physiopathologie)</term><term>Liaison aux protéines</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Solutions</term><term>Syndrome respiratoire aigu sévère (physiopathologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptidyl-Dipeptidase A</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Coronavirus Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Flow Cytometry</term><term>Humans</term><term>Kinetics</term><term>Protein Binding</term><term>Severity of Illness Index</term><term>Solutions</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Cytométrie en flux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Indice de gravité médicale</term><term>Liaison aux protéines</term><term>Solutions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18931070</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>89</Volume><Issue>Pt 11</Issue><PubDate><Year>2008</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2.</ArticleTitle><Pagination><MedlinePgn>2741-2745</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.2008/003962-0</ELocationID><Abstract><AbstractText>Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using a wholly recombinant system, we have investigated the ability of each virus receptor-binding protein, spike or S protein, to bind to ACE-2 in solution and on the cell surface. In both assays, we find that the NL63 S protein has a weaker interaction with ACE-2 than the SARS-CoV S protein, particularly in solution binding, but the residues required for contact are similar. We also confirm that the ACE-2-binding site of NL63 S lies between residues 190 and 739. A lower-affinity interaction with ACE-2 might partly explain the different pathological consequences of infection by SARS-CoV and NL63.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mathewson</LastName><ForeName>Alison C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bishop</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Yongxiu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kemp</LastName><ForeName>Fred</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Junyuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Hongying</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Xiaodong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berkhout</LastName><ForeName>Ben</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van der Hoek</LastName><ForeName>Lia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, K3-110, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Ian M</ForeName><Initials>IM</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Reading, Reading RG6 6AJ, UK.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>G0500597</GrantID><Agency>Medical Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012996">Solutions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike 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PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18931070</ArticleId><ArticleId IdType="doi">10.1099/vir.0.2008/003962-0</ArticleId><ArticleId IdType="pmc">PMC2886958</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Lancet. 2003 Jul 26;362(9380):263-70</Citation><ArticleIdList><ArticleId IdType="pubmed">12892955</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Comp Immunol Microbiol Infect Dis. 2007 Sep;30(5-6):309-27</Citation><ArticleIdList><ArticleId IdType="pubmed">17640731</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Eur J Pediatr. 2008 Jan;167(1):75-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17297612</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>EMBO J. 2005 Apr 20;24(8):1634-43</Citation><ArticleIdList><ArticleId IdType="pubmed">15791205</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Nov;78(21):12090-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15479853</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2006 Sep;80(17):8639-52</Citation><ArticleIdList><ArticleId IdType="pubmed">16912312</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Virol. 2008 Jul;42(3):233-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18406664</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2005 Jul 7;436(7047):112-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16001071</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS J. 2005 Jul;272(14):3512-20</Citation><ArticleIdList><ArticleId IdType="pubmed">16008552</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2006 Feb 10;281(6):3198-203</Citation><ArticleIdList><ArticleId IdType="pubmed">16339146</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2005 Aug;11(8):875-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16007097</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2005 Aug 25;2:73</Citation><ArticleIdList><ArticleId IdType="pubmed">16122388</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Med. 2005 Aug;2(8):e240</Citation><ArticleIdList><ArticleId IdType="pubmed">16104827</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 May 31;102(22):7988-93</Citation><ArticleIdList><ArticleId IdType="pubmed">15897467</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biotechnol. 2002 May 23;95(3):269-75</Citation><ArticleIdList><ArticleId IdType="pubmed">12007867</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2005 Sep 16;309(5742):1864-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16166518</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2004 Apr;10(4):368-73</Citation><ArticleIdList><ArticleId IdType="pubmed">15034574</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2008 Apr;89(Pt 4):1015-1024</Citation><ArticleIdList><ArticleId IdType="pubmed">18343844</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2004 Apr 23;279(17):17996-8007</Citation><ArticleIdList><ArticleId IdType="pubmed">14754895</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antivir Ther. 2007;12(4 Pt B):651-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17944272</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2007 Oct 25;367(2):367-74</Citation><ArticleIdList><ArticleId IdType="pubmed">17631932</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2007 Apr;81(7):3051-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17079323</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Retrovirology. 2007 May 17;4:33</Citation><ArticleIdList><ArticleId IdType="pubmed">17509143</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li><li>Royaume-Uni</li></country><region><li>Hollande-Septentrionale</li></region><settlement><li>Amsterdam</li></settlement><orgName><li>Université d'Amsterdam</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Mathewson, Alison C" sort="Mathewson, Alison C" uniqKey="Mathewson A" first="Alison C" last="Mathewson">Alison C. Mathewson</name></noRegion><name sortKey="Bishop, Alexandra" sort="Bishop, Alexandra" uniqKey="Bishop A" first="Alexandra" last="Bishop">Alexandra Bishop</name><name sortKey="Chen, Hongying" sort="Chen, Hongying" uniqKey="Chen H" first="Hongying" last="Chen">Hongying Chen</name><name sortKey="Jones, Ian M" sort="Jones, Ian M" uniqKey="Jones I" first="Ian M" last="Jones">Ian M. Jones</name><name sortKey="Kemp, Fred" sort="Kemp, Fred" uniqKey="Kemp F" first="Fred" last="Kemp">Fred Kemp</name><name sortKey="Ren, Junyuan" sort="Ren, Junyuan" uniqKey="Ren J" first="Junyuan" last="Ren">Junyuan Ren</name><name sortKey="Xu, Xiaodong" sort="Xu, Xiaodong" uniqKey="Xu X" first="Xiaodong" last="Xu">Xiaodong Xu</name><name sortKey="Yao, Yongxiu" sort="Yao, Yongxiu" uniqKey="Yao Y" first="Yongxiu" last="Yao">Yongxiu Yao</name></country><country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name></region><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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