Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.
Identifieur interne : 001B01 ( PubMed/Checkpoint ); précédent : 001B00; suivant : 001B02Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.
Auteurs : Lanying Du [République populaire de Chine] ; Guangyu Zhao ; Yongping Lin ; Hongyan Sui ; Chris Chan ; Selene Ma ; Yuxian He ; Shibo Jiang ; Changyou Wu ; Kwok-Yung Yuen ; Dong-Yan Jin ; Yusen Zhou ; Bo-Jian ZhengSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 2008.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Animaux, Cellules BALB 3T3, Dependovirus (génétique), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (administration et posologie), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunité muqueuse, Immunoglobuline A (immunologie), Lymphocytes T cytotoxiques (immunologie), Poumon (immunologie), Production d'anticorps, Protéines de l'enveloppe virale (administration et posologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Rate (immunologie), Réplication virale (), Souris, Syndrome respiratoire aigu sévère (), Vaccination, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- administration et posologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux.
- génétique : Dependovirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux.
- immunologie : Glycoprotéines membranaires, Immunoglobuline A, Lymphocytes T cytotoxiques, Poumon, Protéines de l'enveloppe virale, Rate, Vaccins antiviraux.
- physiologie : Virus du SRAS.
- Administration par voie nasale, Animaux, Cellules BALB 3T3, Glycoprotéine de spicule des coronavirus, Humains, Immunité muqueuse, Production d'anticorps, Réplication virale, Souris, Syndrome respiratoire aigu sévère, Vaccination, Virus du SRAS.
English descriptors
- KwdEn :
- Administration, Intranasal, Animals, Antibody Formation, BALB 3T3 Cells, Dependovirus (genetics), Humans, Immunity, Mucosal, Immunoglobulin A (immunology), Lung (immunology), Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, SARS Virus (drug effects), SARS Virus (physiology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Spleen (immunology), T-Lymphocytes, Cytotoxic (immunology), Vaccination, Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology), Virus Replication (drug effects).
- MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Immunoglobulin A, Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- drug effects : SARS Virus, Virus Replication.
- genetics : Dependovirus.
- immunology : Lung, Spleen, T-Lymphocytes, Cytotoxic.
- physiology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Administration, Intranasal, Animals, Antibody Formation, BALB 3T3 Cells, Humans, Immunity, Mucosal, Mice, Spike Glycoprotein, Coronavirus, Vaccination.
Abstract
We have previously reported that a subunit protein vaccine based on the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and a recombinant adeno-associated virus (rAAV)-based RBD (RBD-rAAV) vaccine could induce highly potent neutralizing Ab responses in immunized animals. In this study, systemic, mucosal, and cellular immune responses and long-term protective immunity induced by RBD-rAAV were further characterized in a BALB/c mouse model, with comparison of the i.m. and intranasal (i.n.) routes of administration. Our results demonstrated that: 1) the i.n. vaccination induced a systemic humoral immune response of comparable strength and shorter duration than the i.m. vaccination, but the local humoral immune response was much stronger; 2) the i.n. vaccination elicited stronger systemic and local specific cytotoxic T cell responses than the i.m. vaccination, as evidenced by higher prevalence of IL-2 and/or IFN-gamma-producing CD3+/CD8+ T cells in both lungs and spleen; 3) the i.n. vaccination induced similar protection as the i.m. vaccination against SARS-CoV challenge in mice; 4) higher titers of mucosal IgA and serum-neutralizing Ab were associated with lower viral load and less pulmonary pathological damage, while no Ab-mediated disease enhancement effect was observed; and 5) the vaccination could provide long-term protection against SARS-CoV infection. Taken together, our findings suggest that RBD-rAAV can be further developed into a vaccine candidate for prevention of SARS and that i.n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile.
DOI: 10.4049/jimmunol.180.2.948
PubMed: 18178835
Affiliations:
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pubmed:18178835Le document en format XML
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<term>Humans</term>
<term>Immunity, Mucosal</term>
<term>Immunoglobulin A (immunology)</term>
<term>Lung (immunology)</term>
<term>Membrane Glycoproteins (administration & dosage)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
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<term>SARS Virus (physiology)</term>
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<term>Spike Glycoprotein, Coronavirus</term>
<term>Spleen (immunology)</term>
<term>T-Lymphocytes, Cytotoxic (immunology)</term>
<term>Vaccination</term>
<term>Viral Envelope Proteins (administration & dosage)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
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<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (administration et posologie)</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Humains</term>
<term>Immunité muqueuse</term>
<term>Immunoglobuline A (immunologie)</term>
<term>Lymphocytes T cytotoxiques (immunologie)</term>
<term>Poumon (immunologie)</term>
<term>Production d'anticorps</term>
<term>Protéines de l'enveloppe virale (administration et posologie)</term>
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<term>Vaccins antiviraux (immunologie)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Viral Vaccines</term>
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<term>Viral Vaccines</term>
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<term>Vaccins antiviraux</term>
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<term>Vaccins antiviraux</term>
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<term>Lymphocytes T cytotoxiques</term>
<term>Poumon</term>
<term>Protéines de l'enveloppe virale</term>
<term>Rate</term>
<term>Vaccins antiviraux</term>
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<term>Antibody Formation</term>
<term>BALB 3T3 Cells</term>
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<front><div type="abstract" xml:lang="en">We have previously reported that a subunit protein vaccine based on the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and a recombinant adeno-associated virus (rAAV)-based RBD (RBD-rAAV) vaccine could induce highly potent neutralizing Ab responses in immunized animals. In this study, systemic, mucosal, and cellular immune responses and long-term protective immunity induced by RBD-rAAV were further characterized in a BALB/c mouse model, with comparison of the i.m. and intranasal (i.n.) routes of administration. Our results demonstrated that: 1) the i.n. vaccination induced a systemic humoral immune response of comparable strength and shorter duration than the i.m. vaccination, but the local humoral immune response was much stronger; 2) the i.n. vaccination elicited stronger systemic and local specific cytotoxic T cell responses than the i.m. vaccination, as evidenced by higher prevalence of IL-2 and/or IFN-gamma-producing CD3+/CD8+ T cells in both lungs and spleen; 3) the i.n. vaccination induced similar protection as the i.m. vaccination against SARS-CoV challenge in mice; 4) higher titers of mucosal IgA and serum-neutralizing Ab were associated with lower viral load and less pulmonary pathological damage, while no Ab-mediated disease enhancement effect was observed; and 5) the vaccination could provide long-term protection against SARS-CoV infection. Taken together, our findings suggest that RBD-rAAV can be further developed into a vaccine candidate for prevention of SARS and that i.n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile.</div>
</front>
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<DateCompleted><Year>2008</Year>
<Month>03</Month>
<Day>10</Day>
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<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>16</Day>
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<JournalIssue CitedMedium="Print"><Volume>180</Volume>
<Issue>2</Issue>
<PubDate><Year>2008</Year>
<Month>Jan</Month>
<Day>15</Day>
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<Title>Journal of immunology (Baltimore, Md. : 1950)</Title>
<ISOAbbreviation>J. Immunol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.</ArticleTitle>
<Pagination><MedlinePgn>948-56</MedlinePgn>
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<Abstract><AbstractText>We have previously reported that a subunit protein vaccine based on the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and a recombinant adeno-associated virus (rAAV)-based RBD (RBD-rAAV) vaccine could induce highly potent neutralizing Ab responses in immunized animals. In this study, systemic, mucosal, and cellular immune responses and long-term protective immunity induced by RBD-rAAV were further characterized in a BALB/c mouse model, with comparison of the i.m. and intranasal (i.n.) routes of administration. Our results demonstrated that: 1) the i.n. vaccination induced a systemic humoral immune response of comparable strength and shorter duration than the i.m. vaccination, but the local humoral immune response was much stronger; 2) the i.n. vaccination elicited stronger systemic and local specific cytotoxic T cell responses than the i.m. vaccination, as evidenced by higher prevalence of IL-2 and/or IFN-gamma-producing CD3+/CD8+ T cells in both lungs and spleen; 3) the i.n. vaccination induced similar protection as the i.m. vaccination against SARS-CoV challenge in mice; 4) higher titers of mucosal IgA and serum-neutralizing Ab were associated with lower viral load and less pulmonary pathological damage, while no Ab-mediated disease enhancement effect was observed; and 5) the vaccination could provide long-term protection against SARS-CoV infection. Taken together, our findings suggest that RBD-rAAV can be further developed into a vaccine candidate for prevention of SARS and that i.n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName>
<ForeName>Lanying</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Guangyu</ForeName>
<Initials>G</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Yongping</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Sui</LastName>
<ForeName>Hongyan</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Chris</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ma</LastName>
<ForeName>Selene</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Yuxian</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Shibo</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Changyou</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yuen</LastName>
<ForeName>Kwok-Yung</ForeName>
<Initials>KY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jin</LastName>
<ForeName>Dong-Yan</ForeName>
<Initials>DY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Yusen</ForeName>
<Initials>Y</Initials>
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<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Bo-Jian</ForeName>
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