Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.
Identifieur interne : 001A17 ( PubMed/Checkpoint ); précédent : 001A16; suivant : 001A18Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.
Auteurs : Chenxiang Lin [États-Unis] ; Jeanette K. Nangreave ; Zhe Li ; Yan Liu ; Hao YanSource :
- Nanomedicine (London, England) [ 1748-6963 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN, ADN viral, Adénosine triphosphate.
- génétique : ADN, ADN viral, Virus du SRAS.
- Nanotechnologie, Reproductibilité des résultats, Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Adenosine Triphosphate, DNA, DNA, Viral.
- chemical , genetics : DNA, DNA, Viral.
- genetics : SARS Virus.
- methods : Biosensing Techniques, Nanotechnology.
- Reproducibility of Results.
Abstract
Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.
DOI: 10.2217/17435889.3.4.521
PubMed: 18694314
Affiliations:
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Nangreave</name></author><author><name sortKey="Li, Zhe" sort="Li, Zhe" uniqKey="Li Z" first="Zhe" last="Li">Zhe Li</name></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name></author><author><name sortKey="Yan, Hao" sort="Yan, Hao" uniqKey="Yan H" first="Hao" last="Yan">Hao Yan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18694314</idno><idno type="pmid">18694314</idno><idno type="doi">10.2217/17435889.3.4.521</idno><idno type="wicri:Area/PubMed/Corpus">001A88</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A88</idno><idno type="wicri:Area/PubMed/Curation">001A88</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A88</idno><idno type="wicri:Area/PubMed/Checkpoint">001A17</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.</title><author><name sortKey="Lin, Chenxiang" sort="Lin, Chenxiang" uniqKey="Lin C" first="Chenxiang" last="Lin">Chenxiang Lin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Nangreave, Jeanette K" sort="Nangreave, Jeanette K" uniqKey="Nangreave J" first="Jeanette K" last="Nangreave">Jeanette K. Nangreave</name></author><author><name sortKey="Li, Zhe" sort="Li, Zhe" uniqKey="Li Z" first="Zhe" last="Li">Zhe Li</name></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name></author><author><name sortKey="Yan, Hao" sort="Yan, Hao" uniqKey="Yan H" first="Hao" last="Yan">Hao Yan</name></author></analytic><series><title level="j">Nanomedicine (London, England)</title><idno type="eISSN">1748-6963</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (analysis)</term><term>Biosensing Techniques (methods)</term><term>DNA (analysis)</term><term>DNA (genetics)</term><term>DNA, Viral (analysis)</term><term>DNA, Viral (genetics)</term><term>Nanotechnology (methods)</term><term>Reproducibility of Results</term><term>SARS Virus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>ADN (génétique)</term><term>ADN viral (analyse)</term><term>ADN viral (génétique)</term><term>Adénosine triphosphate (analyse)</term><term>Nanotechnologie ()</term><term>Reproductibilité des résultats</term><term>Techniques de biocapteur ()</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Adenosine Triphosphate</term><term>DNA</term><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term><term>ADN viral</term><term>Adénosine triphosphate</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>ADN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Nanotechnology</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Nanotechnologie</term><term>Reproductibilité des résultats</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18694314</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>06</Day></DateCompleted><DateRevised><Year>2014</Year><Month>07</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1748-6963</ISSN><JournalIssue CitedMedium="Internet"><Volume>3</Volume><Issue>4</Issue><PubDate><Year>2008</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Nanomedicine (London, England)</Title><ISOAbbreviation>Nanomedicine (Lond)</ISOAbbreviation></Journal><ArticleTitle>Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.</ArticleTitle><Pagination><MedlinePgn>521-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2217/17435889.3.4.521</ELocationID><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.</AbstractText><AbstractText Label="MATERIALS & METHODS" NlmCategory="METHODS">The fluorescence enhancement on the addition of specific detection targets was observed directly by confocal fluorescence microscopy.</AbstractText><AbstractText Label="RESULTS & DISCUSSION" NlmCategory="CONCLUSIONS">The versatility of the system was demonstrated by successful detection of SARS viral DNA and ATP. Improvement of sensitivity by two orders of magnitude was achieved for both targets, compared with our previously reported system without signal amplification.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">In summary, this work provides proof-of-concept that HCR can occur efficiently on DNA-tile-based nanoarrays, thus facilitating more sensitive detection. The merging of HCR with combinatorial encoding systems to realize highly sensitive and multiplexed biosensing may provide new tools for nanomedical applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Chenxiang</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nangreave</LastName><ForeName>Jeanette K</ForeName><Initials>JK</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhe</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Hao</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1R21 EB0005844-01</GrantID><Acronym>EB</Acronym><Agency>NIBIB NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanomedicine (Lond)</MedlineTA><NlmUniqueID>101278111</NlmUniqueID><ISSNLinking>1743-5889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>8L70Q75FXE</RegistryNumber><NameOfSubstance UI="D000255">Adenosine Triphosphate</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000255" MajorTopicYN="N">Adenosine Triphosphate</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D036103" MajorTopicYN="N">Nanotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>8</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>8</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18694314</ArticleId><ArticleId IdType="doi">10.2217/17435889.3.4.521</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Arizona</li></region></list><tree><noCountry><name sortKey="Li, Zhe" sort="Li, Zhe" uniqKey="Li Z" first="Zhe" last="Li">Zhe Li</name><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><name sortKey="Nangreave, Jeanette K" sort="Nangreave, Jeanette K" uniqKey="Nangreave J" first="Jeanette K" last="Nangreave">Jeanette K. Nangreave</name><name sortKey="Yan, Hao" sort="Yan, Hao" uniqKey="Yan H" first="Hao" last="Yan">Hao Yan</name></noCountry><country name="États-Unis"><region name="Arizona"><name sortKey="Lin, Chenxiang" sort="Lin, Chenxiang" uniqKey="Lin C" first="Chenxiang" last="Lin">Chenxiang Lin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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