Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.
Identifieur interne : 001A17 ( PubMed/Checkpoint ); précédent : 001A16; suivant : 001A18Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.
Auteurs : Chenxiang Lin [États-Unis] ; Jeanette K. Nangreave ; Zhe Li ; Yan Liu ; Hao YanSource :
- Nanomedicine (London, England) [ 1748-6963 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN, ADN viral, Adénosine triphosphate.
- génétique : ADN, ADN viral, Virus du SRAS.
- Nanotechnologie, Reproductibilité des résultats, Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Adenosine Triphosphate, DNA, DNA, Viral.
- chemical , genetics : DNA, DNA, Viral.
- genetics : SARS Virus.
- methods : Biosensing Techniques, Nanotechnology.
- Reproducibility of Results.
Abstract
Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.
DOI: 10.2217/17435889.3.4.521
PubMed: 18694314
Affiliations:
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pubmed:18694314Le document en format XML
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<author><name sortKey="Lin, Chenxiang" sort="Lin, Chenxiang" uniqKey="Lin C" first="Chenxiang" last="Lin">Chenxiang Lin</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287</wicri:regionArea>
<placeName><region type="state">Arizona</region>
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<author><name sortKey="Nangreave, Jeanette K" sort="Nangreave, Jeanette K" uniqKey="Nangreave J" first="Jeanette K" last="Nangreave">Jeanette K. Nangreave</name>
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<author><name sortKey="Li, Zhe" sort="Li, Zhe" uniqKey="Li Z" first="Zhe" last="Li">Zhe Li</name>
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<author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name>
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<author><name sortKey="Yan, Hao" sort="Yan, Hao" uniqKey="Yan H" first="Hao" last="Yan">Hao Yan</name>
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<author><name sortKey="Li, Zhe" sort="Li, Zhe" uniqKey="Li Z" first="Zhe" last="Li">Zhe Li</name>
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<series><title level="j">Nanomedicine (London, England)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (analysis)</term>
<term>Biosensing Techniques (methods)</term>
<term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>DNA, Viral (analysis)</term>
<term>DNA, Viral (genetics)</term>
<term>Nanotechnology (methods)</term>
<term>Reproducibility of Results</term>
<term>SARS Virus (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (génétique)</term>
<term>ADN viral (analyse)</term>
<term>ADN viral (génétique)</term>
<term>Adénosine triphosphate (analyse)</term>
<term>Nanotechnologie ()</term>
<term>Reproductibilité des résultats</term>
<term>Techniques de biocapteur ()</term>
<term>Virus du SRAS (génétique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Adenosine Triphosphate</term>
<term>DNA</term>
<term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
<term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
<term>ADN viral</term>
<term>Adénosine triphosphate</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term>
<term>ADN viral</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
<term>Nanotechnology</term>
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<keywords scheme="MESH" xml:lang="en"><term>Reproducibility of Results</term>
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<front><div type="abstract" xml:lang="en">Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.</div>
</front>
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<DateCompleted><Year>2009</Year>
<Month>01</Month>
<Day>06</Day>
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<DateRevised><Year>2014</Year>
<Month>07</Month>
<Day>30</Day>
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<Issue>4</Issue>
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<Title>Nanomedicine (London, England)</Title>
<ISOAbbreviation>Nanomedicine (Lond)</ISOAbbreviation>
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<ArticleTitle>Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.</ArticleTitle>
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<Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes.</AbstractText>
<AbstractText Label="MATERIALS & METHODS" NlmCategory="METHODS">The fluorescence enhancement on the addition of specific detection targets was observed directly by confocal fluorescence microscopy.</AbstractText>
<AbstractText Label="RESULTS & DISCUSSION" NlmCategory="CONCLUSIONS">The versatility of the system was demonstrated by successful detection of SARS viral DNA and ATP. Improvement of sensitivity by two orders of magnitude was achieved for both targets, compared with our previously reported system without signal amplification.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">In summary, this work provides proof-of-concept that HCR can occur efficiently on DNA-tile-based nanoarrays, thus facilitating more sensitive detection. The merging of HCR with combinatorial encoding systems to realize highly sensitive and multiplexed biosensing may provide new tools for nanomedical applications.</AbstractText>
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<Author ValidYN="Y"><LastName>Yan</LastName>
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