Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals.
Identifieur interne : 001792 ( PubMed/Checkpoint ); précédent : 001791; suivant : 001793Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals.
Auteurs : Junbao Yang [États-Unis] ; Eddie James ; Michelle Roti ; Laurie Huston ; John A. Gebe ; William W. KwokSource :
- International immunology [ 1460-2377 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Antigènes d'histocompatibilité de classe II (immunologie), Antigènes d'histocompatibilité de classe II (métabolisme), Cartographie épitopique, Cytokines (biosynthèse), Cytokines (immunologie), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Lymphocytes T CD4+ (immunologie), Lymphocytes T CD4+ (métabolisme), Lymphocytes T CD4+ (virologie), Prolifération cellulaire, Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Souris, Souris transgéniques, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Séquence d'acides aminés, Virus du SRAS (immunologie), Virus du SRAS (métabolisme), Épitopes immunodominants (immunologie), Épitopes immunodominants (métabolisme).
- MESH :
- biosynthèse : Cytokines.
- immunologie : Antigènes d'histocompatibilité de classe II, Cytokines, Glycoprotéines membranaires, Lymphocytes T CD4+, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Virus du SRAS, Épitopes immunodominants.
- métabolisme : Antigènes d'histocompatibilité de classe II, Glycoprotéines membranaires, Lymphocytes T CD4+, Protéines de l'enveloppe virale, Virus du SRAS, Épitopes immunodominants.
- virologie : Lymphocytes T CD4+, Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Animaux, Cartographie épitopique, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Prolifération cellulaire, Souris, Souris transgéniques, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, CD4-Positive T-Lymphocytes (immunology), CD4-Positive T-Lymphocytes (metabolism), CD4-Positive T-Lymphocytes (virology), Cell Proliferation, Cytokines (biosynthesis), Cytokines (immunology), Epitope Mapping, Histocompatibility Antigens Class II (immunology), Histocompatibility Antigens Class II (metabolism), Humans, Immunodominant Epitopes (immunology), Immunodominant Epitopes (metabolism), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Transgenic, Molecular Sequence Data, SARS Virus (immunology), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , biosynthesis : Cytokines.
- epidemiology : Severe Acute Respiratory Syndrome.
- immunology : CD4-Positive T-Lymphocytes, Cytokines, Histocompatibility Antigens Class II, Immunodominant Epitopes, Membrane Glycoproteins, SARS Virus, Severe Acute Respiratory Syndrome, Viral Envelope Proteins.
- metabolism : CD4-Positive T-Lymphocytes, Histocompatibility Antigens Class II, Immunodominant Epitopes, Membrane Glycoproteins, SARS Virus, Viral Envelope Proteins.
- virology : CD4-Positive T-Lymphocytes, Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Cell Proliferation, Epitope Mapping, Humans, Mice, Mice, Transgenic, Molecular Sequence Data, Spike Glycoprotein, Coronavirus.
Abstract
Identification of dominant T cell epitopes within newly emerging and re-emerging infectious organisms is valuable in understanding pathogenic immune responses and potential vaccine designs. However, difficulties in obtaining samples from patients or convalescent subjects have hampered research in this direction. We demonstrated a strategy, tetramer-guided epitope mapping, that specific CD4+ T cell epitopes can be identified by using PBMC from subjects that have not been exposed to the infectious organism. Sixteen HLA-DR0401- and 14 HLA-DR0701-restricted epitopes within spike protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) were identified. Among these, spike protein residues 159-171, 166-178, 449-461 and 1083-1097 were identified to contain naturally processed immunodominant epitopes based on strong in vitro T cell responses of PBMC (as assayed by tetramer staining) to intact spike protein stimulation. These immunodominant epitopes were confirmed in vivo in HLA-DR0401 transgenic mice by immunizing with spike protein. Furthermore, the epitope-specific T cells from naive donors secreted IFN-gamma and IL-13 upon re-stimulation with corresponding tetramers. Our study demonstrates a strategy to determine potential immunodominant epitopes for emerging infectious pathogens prior to their epidemic circulation.
DOI: 10.1093/intimm/dxn124
PubMed: 19050106
Affiliations:
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pubmed:19050106Le document en format XML
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Sequence</term><term>Animals</term><term>Cell Proliferation</term><term>Epitope Mapping</term><term>Humans</term><term>Mice</term><term>Mice, Transgenic</term><term>Molecular Sequence Data</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie épitopique</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Identification of dominant T cell epitopes within newly emerging and re-emerging infectious organisms is valuable in understanding pathogenic immune responses and potential vaccine designs. However, difficulties in obtaining samples from patients or convalescent subjects have hampered research in this direction. We demonstrated a strategy, tetramer-guided epitope mapping, that specific CD4+ T cell epitopes can be identified by using PBMC from subjects that have not been exposed to the infectious organism. Sixteen HLA-DR0401- and 14 HLA-DR0701-restricted epitopes within spike protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) were identified. Among these, spike protein residues 159-171, 166-178, 449-461 and 1083-1097 were identified to contain naturally processed immunodominant epitopes based on strong in vitro T cell responses of PBMC (as assayed by tetramer staining) to intact spike protein stimulation. These immunodominant epitopes were confirmed in vivo in HLA-DR0401 transgenic mice by immunizing with spike protein. Furthermore, the epitope-specific T cells from naive donors secreted IFN-gamma and IL-13 upon re-stimulation with corresponding tetramers. Our study demonstrates a strategy to determine potential immunodominant epitopes for emerging infectious pathogens prior to their epidemic circulation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19050106</PMID><DateCompleted><Year>2009</Year><Month>08</Month><Day>31</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2377</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>International immunology</Title><ISOAbbreviation>Int. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals.</ArticleTitle><Pagination><MedlinePgn>63-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/intimm/dxn124</ELocationID><Abstract><AbstractText>Identification of dominant T cell epitopes within newly emerging and re-emerging infectious organisms is valuable in understanding pathogenic immune responses and potential vaccine designs. However, difficulties in obtaining samples from patients or convalescent subjects have hampered research in this direction. We demonstrated a strategy, tetramer-guided epitope mapping, that specific CD4+ T cell epitopes can be identified by using PBMC from subjects that have not been exposed to the infectious organism. Sixteen HLA-DR0401- and 14 HLA-DR0701-restricted epitopes within spike protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) were identified. Among these, spike protein residues 159-171, 166-178, 449-461 and 1083-1097 were identified to contain naturally processed immunodominant epitopes based on strong in vitro T cell responses of PBMC (as assayed by tetramer staining) to intact spike protein stimulation. These immunodominant epitopes were confirmed in vivo in HLA-DR0401 transgenic mice by immunizing with spike protein. Furthermore, the epitope-specific T cells from naive donors secreted IFN-gamma and IL-13 upon re-stimulation with corresponding tetramers. Our study demonstrates a strategy to determine potential immunodominant epitopes for emerging infectious pathogens prior to their epidemic circulation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Junbao</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>James</LastName><ForeName>Eddie</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Roti</LastName><ForeName>Michelle</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Huston</LastName><ForeName>Laurie</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Gebe</LastName><ForeName>John A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Kwok</LastName><ForeName>William W</ForeName><Initials>WW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>12</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Int Immunol</MedlineTA><NlmUniqueID>8916182</NlmUniqueID><ISSNLinking>0953-8178</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000949">Histocompatibility Antigens Class II</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016056">Immunodominant Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018604" MajorTopicYN="N">Epitope Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000949" MajorTopicYN="N">Histocompatibility Antigens Class II</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016056" MajorTopicYN="N">Immunodominant Epitopes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008822" MajorTopicYN="N">Mice, Transgenic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>12</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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(État)</li></region></list><tree><noCountry><name sortKey="Gebe, John A" sort="Gebe, John A" uniqKey="Gebe J" first="John A" last="Gebe">John A. Gebe</name><name sortKey="Huston, Laurie" sort="Huston, Laurie" uniqKey="Huston L" first="Laurie" last="Huston">Laurie Huston</name><name sortKey="James, Eddie" sort="James, Eddie" uniqKey="James E" first="Eddie" last="James">Eddie James</name><name sortKey="Kwok, William W" sort="Kwok, William W" uniqKey="Kwok W" first="William W" last="Kwok">William W. Kwok</name><name sortKey="Roti, Michelle" sort="Roti, Michelle" uniqKey="Roti M" first="Michelle" last="Roti">Michelle Roti</name></noCountry><country name="États-Unis"><region name="Washington (État)"><name sortKey="Yang, Junbao" sort="Yang, Junbao" uniqKey="Yang J" first="Junbao" last="Yang">Junbao Yang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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