Profiling of substrate specificities of 3C-like proteases from group 1, 2a, 2b, and 3 coronaviruses.
Identifieur interne : 001451 ( PubMed/Checkpoint ); précédent : 001450; suivant : 001452Profiling of substrate specificities of 3C-like proteases from group 1, 2a, 2b, and 3 coronaviruses.
Auteurs : Chi-Pang Chuck [République populaire de Chine] ; Hak-Fun Chow ; David Chi-Cheong Wan ; Kam-Bo WongSource :
- PloS one [ 1932-6203 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Coronavirus.
- génétique : Peptide hydrolases.
- métabolisme : Peptide hydrolases.
- Clonage moléculaire, Coronavirus, Données de séquences moléculaires, Modèles moléculaires, Spécificité du substrat.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Peptide Hydrolases.
- classification : Coronavirus.
- enzymology : Coronavirus.
- chemical , metabolism : Peptide Hydrolases.
- Cloning, Molecular, Models, Molecular, Molecular Sequence Data, Substrate Specificity.
Abstract
Coronaviruses (CoVs) can be classified into alphacoronavirus (group 1), betacoronavirus (group 2), and gammacoronavirus (group 3) based on diversity of the protein sequences. Their 3C-like protease (3CL(pro)), which catalyzes the proteolytic processing of the polyproteins for viral replication, is a potential target for anti-coronaviral infection.
DOI: 10.1371/journal.pone.0027228
PubMed: 22073294
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:22073294Le document en format XML
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<term>Molecular Sequence Data</term>
<term>Peptide Hydrolases (genetics)</term>
<term>Peptide Hydrolases (metabolism)</term>
<term>Substrate Specificity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire</term>
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<term>Modèles moléculaires</term>
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<term>Peptide hydrolases (métabolisme)</term>
<term>Spécificité du substrat</term>
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<front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) can be classified into alphacoronavirus (group 1), betacoronavirus (group 2), and gammacoronavirus (group 3) based on diversity of the protein sequences. Their 3C-like protease (3CL(pro)), which catalyzes the proteolytic processing of the polyproteins for viral replication, is a potential target for anti-coronaviral infection.</div>
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<Title>PloS one</Title>
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<ArticleTitle>Profiling of substrate specificities of 3C-like proteases from group 1, 2a, 2b, and 3 coronaviruses.</ArticleTitle>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Coronaviruses (CoVs) can be classified into alphacoronavirus (group 1), betacoronavirus (group 2), and gammacoronavirus (group 3) based on diversity of the protein sequences. Their 3C-like protease (3CL(pro)), which catalyzes the proteolytic processing of the polyproteins for viral replication, is a potential target for anti-coronaviral infection.</AbstractText>
<AbstractText Label="METHODOLOGY/PRINCIPAL FINDINGS" NlmCategory="RESULTS">Here, we profiled the substrate specificities of 3CL(pro) from human CoV NL63 (group 1), human CoV OC43 (group 2a), severe acute respiratory syndrome coronavirus (SARS-CoV) (group 2b) and infectious bronchitis virus (IBV) (group 3), by measuring their activity against a substrate library of 19 × 8 of variants with single substitutions at P5 to P3' positions. The results were correlated with structural properties like side chain volume, hydrophobicity, and secondary structure propensities of substituting residues. All 3CL(pro) prefer Gln at P1 position, Leu at P2 position, basic residues at P3 position, small hydrophobic residues at P4 position, and small residues at P1' and P2' positions. Despite 3CL(pro) from different groups of CoVs share many similarities in substrate specificities, differences in substrate specificities were observed at P4 positions, with IBV 3CL(pro) prefers P4-Pro and SARS-CoV 3CL(pro) prefers P4-Val. By combining the most favorable residues at P3 to P5 positions, we identified super-active substrate sequences 'VARLQ↓SGF' that can be cleaved efficiently by all 3CL(pro) with relative activity of 1.7 to 3.2, and 'VPRLQ↓SGF' that can be cleaved specifically by IBV 3CL(pro) with relative activity of 4.3.</AbstractText>
<AbstractText Label="CONCLUSIONS/SIGNIFICANCE" NlmCategory="CONCLUSIONS">The comprehensive substrate specificities of 3CL(pro) from each of the group 1, 2a, 2b, and 3 CoVs have been profiled in this study, which may provide insights into a rational design of broad-spectrum peptidomimetic inhibitors targeting the proteases.</AbstractText>
</Abstract>
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<ForeName>Chi-Pang</ForeName>
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<ReferenceList><Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1403-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690906</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Feb;79(4):2001-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15681402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Jun;79(11):7095-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890949</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Virol. 2009 Sep;81(9):1597-604</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19626607</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(10):e13197</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20949131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biophys Chem. 2008 May;134(3):185-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18342425</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1982 May 5;157(1):105-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7108955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2005 Oct;3(10):e324</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16128623</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Jan;79(2):884-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15613317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2005 Jul;67(1):18-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15885816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Res. 2007 Mar-Apr;38(2):281-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17296157</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Jul 26;362(9380):263-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12892955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 1949 Sep;90(3):181-210</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18137294</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Rev. 1992 Mar;56(1):61-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1579113</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Soc Exp Biol Med. 1966 Jan;121(1):190-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4285768</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2007 Jul 31;46(30):8744-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17605471</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2004 Sep 10;574(1-3):131-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15358553</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Annu Meet U S Anim Health Assoc. 1974;(78):359-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4377955</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Rev Sci Tech. 2000 Aug;19(2):493-508</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10935276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Aug;78(15):7863-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15254158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Evol. 2002 Feb;54(2):156-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11821909</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Jun 13;300(5626):1763-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12746549</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Biol Med (Maywood). 2009 Oct;234(10):1117-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19546349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 1966 Jan 8;1(7428):76-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4158999</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2005 Apr 15;329(3):934-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15752746</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2004 Apr;10(4):368-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15034574</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2002 Mar;83(Pt 3):595-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11842254</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Vet Med Assoc. 1946 Apr;108:257-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21020443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Sep;82(17):8647-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18562531</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148</Citation>
<ArticleIdList><ArticleId IdType="pubmed">364941</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2008 Apr;9(4):267-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18319742</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2011 Nov;92(2):204-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21854807</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10012-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15226499</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Mar;82(5):2515-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18094151</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2010 Aug;48(8):2940-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20554810</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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