Coronavirus reverse genetic systems: infectious clones and replicons.
Identifieur interne : 001026 ( PubMed/Checkpoint ); précédent : 001025; suivant : 001027Coronavirus reverse genetic systems: infectious clones and replicons.
Auteurs : Fernando Almazán [Espagne] ; Isabel Sola [Espagne] ; Sonia Zu Iga [Espagne] ; Silvia Marquez-Jurado [Espagne] ; Lucia Morales [Espagne] ; Martina Becares [Espagne] ; Luis Enjuanes [Espagne]Source :
- Virus research [ 1872-7492 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Coronavirus.
- Clones cellulaires, Génétique inverse, Réplicon, Virologie.
English descriptors
- KwdEn :
- MESH :
- genetics : Coronavirus.
- methods : Reverse Genetics, Virology.
- Clone Cells, Replicon.
Abstract
Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV).
DOI: 10.1016/j.virusres.2014.05.026
PubMed: 24930446
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:24930446Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Coronavirus reverse genetic systems: infectious clones and replicons.</title>
<author><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Marquez-Jurado">Silvia Marquez-Jurado</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Morales, Lucia" sort="Morales, Lucia" uniqKey="Morales L" first="Lucia" last="Morales">Lucia Morales</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Becares, Martina" sort="Becares, Martina" uniqKey="Becares M" first="Martina" last="Becares">Martina Becares</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain. Electronic address: L.Enjuanes@cnb.csic.es.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:24930446</idno>
<idno type="pmid">24930446</idno>
<idno type="doi">10.1016/j.virusres.2014.05.026</idno>
<idno type="wicri:Area/PubMed/Corpus">000F81</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000F81</idno>
<idno type="wicri:Area/PubMed/Curation">000F81</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000F81</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001026</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001026</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Coronavirus reverse genetic systems: infectious clones and replicons.</title>
<author><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Marquez-Jurado">Silvia Marquez-Jurado</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Morales, Lucia" sort="Morales, Lucia" uniqKey="Morales L" first="Lucia" last="Morales">Lucia Morales</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Becares, Martina" sort="Becares, Martina" uniqKey="Becares M" first="Martina" last="Becares">Martina Becares</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain. Electronic address: L.Enjuanes@cnb.csic.es.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
<settlement type="city">Madrid</settlement>
</placeName>
<orgName type="university">Université autonome de Madrid</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">Virus research</title>
<idno type="eISSN">1872-7492</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clone Cells</term>
<term>Coronavirus (genetics)</term>
<term>Replicon</term>
<term>Reverse Genetics (methods)</term>
<term>Virology (methods)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Clones cellulaires</term>
<term>Coronavirus (génétique)</term>
<term>Génétique inverse ()</term>
<term>Réplicon</term>
<term>Virologie ()</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Genetics</term>
<term>Virology</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term>
<term>Replicon</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Clones cellulaires</term>
<term>Génétique inverse</term>
<term>Réplicon</term>
<term>Virologie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV). </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24930446</PMID>
<DateCompleted><Year>2015</Year>
<Month>04</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7492</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>189</Volume>
<PubDate><Year>2014</Year>
<Month>Aug</Month>
<Day>30</Day>
</PubDate>
</JournalIssue>
<Title>Virus research</Title>
<ISOAbbreviation>Virus Res.</ISOAbbreviation>
</Journal>
<ArticleTitle>Coronavirus reverse genetic systems: infectious clones and replicons.</ArticleTitle>
<Pagination><MedlinePgn>262-70</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.virusres.2014.05.026</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S0168-1702(14)00240-8</ELocationID>
<Abstract><AbstractText>Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some CoV replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome CoV (SARS-CoV). </AbstractText>
<CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Almazán</LastName>
<ForeName>Fernando</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sola</LastName>
<ForeName>Isabel</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zuñiga</LastName>
<ForeName>Sonia</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Marquez-Jurado</LastName>
<ForeName>Silvia</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Morales</LastName>
<ForeName>Lucia</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Becares</LastName>
<ForeName>Martina</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Enjuanes</LastName>
<ForeName>Luis</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology. Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, C/ Darwin 3, Cantoblanco, 28049 Madrid, Spain. Electronic address: L.Enjuanes@cnb.csic.es.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>P01 AI060699</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D016454">Review</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>06</Month>
<Day>12</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Virus Res</MedlineTA>
<NlmUniqueID>8410979</NlmUniqueID>
<ISSNLinking>0168-1702</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList><CommentsCorrections RefType="ReprintIn"><RefSource>Virus Res. 2014 Dec 19;194:67-75</RefSource>
<PMID Version="1">25261606</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D002999" MajorTopicYN="N">Clone Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012093" MajorTopicYN="N">Replicon</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059386" MajorTopicYN="N">Reverse Genetics</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014773" MajorTopicYN="N">Virology</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Coronavirus</Keyword>
<Keyword MajorTopicYN="N">Infectious clones</Keyword>
<Keyword MajorTopicYN="N">Replicons</Keyword>
<Keyword MajorTopicYN="N">Reverse genetics</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>04</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2014</Year>
<Month>05</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2014</Year>
<Month>05</Month>
<Day>28</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>6</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>6</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>4</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">24930446</ArticleId>
<ArticleId IdType="pii">S0168-1702(14)00240-8</ArticleId>
<ArticleId IdType="doi">10.1016/j.virusres.2014.05.026</ArticleId>
<ArticleId IdType="pmc">PMC4727449</ArticleId>
<ArticleId IdType="mid">NIHMS751470</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nucleic Acids Res. 2006;34(17):4816-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16971454</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Jun;87(12):6551-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23576515</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2009 Aug 24;6:131</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19698190</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2008 Aug;82(15):7721-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18463152</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Future Virol. 2011 May;6(5):615-631</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21765859</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Microbiol. 2003 Dec;1(3):209-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15035025</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2001 Jun;82(Pt 6):1273-1281</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11369870</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2010 Mar 30;399(1):120-128</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20110095</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Virus Res. 1997;48:1-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9233431</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Can J Microbiol. 2009 Mar;55(3):254-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19370068</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 1996 May;77 ( Pt 5):963-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8609493</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Jul;81(13):7086-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17428870</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8794-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1528894</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antimicrob Agents Chemother. 2012 Sep;56(9):4718-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22733076</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol Methods. 2005 Feb;123(2):203-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15620403</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2008 Apr;82(8):3882-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18272586</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2008 Oct 01;3(10):e3299</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18827877</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2008 Dec;82(23):11948-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18818320</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2010 Oct;84(19):10148-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20668085</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 May;76(9):4655-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11932433</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2012 Jun;86(12):6994-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22491466</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antiviral Res. 2008 Nov;80(2):107-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18584889</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2008 Sep 12;374(1):138-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18619943</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Oct;75(19):9059-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11533169</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>mBio. 2013 Sep 10;4(5):e00650-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24023385</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biotechniques. 2006 Feb;40(2):191-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16526409</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2000 May 9;97(10):5516-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10805807</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1994 Nov;68(11):7458-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7933129</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Nov;76(21):11065-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12368349</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Jun;79(11):6620-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890900</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1996 Jan;70(1):508-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8523564</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Gene. 1983 Nov;25(1):21-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6229451</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Top Microbiol Immunol. 2005;287:199-227</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15609513</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Oct;73(10):8349-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10482585</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1998 Oct;72(10):7926-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9733830</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Top Microbiol Immunol. 2005;287:229-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15609514</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2007 Mar 30;360(1):150-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17098272</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1987 Jun;61(6):1788-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3573150</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Feb;76(3):1422-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11773416</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Annu Rev Microbiol. 2006;60:211-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16712436</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antiviral Res. 2014 Jan;101:105-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24269477</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2012;7(3):e32857</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22412934</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2012 Jun;86(11):6258-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22438554</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2010 Jun;84(12):6050-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20392858</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19944-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19036930</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Virus Res. 2006;66:193-292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16877062</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):9977-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1438247</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Feb;81(4):1701-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17108030</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2004 Nov;78(22):12683-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15507657</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2013 Apr 08;8(4):e61166</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23593419</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1986 Mar;57(3):729-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3005623</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Dec;75(24):12359-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11711626</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Drug Discov. 2002 Nov;1(11):911-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12415250</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Nov;76(22):11518-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12388713</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2007 Feb 5;358(1):136-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16979681</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antiviral Res. 2011 Jul;91(1):1-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21549154</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2009 Apr;83(8):3743-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19176619</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Genomics. 2001 Apr 1;73(1):56-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11352566</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Keio J Med. 2008 Jun;57(2):75-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18677087</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Methods Mol Biol. 2008;454:237-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19057873</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12995-3000</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14569023</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12546-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16891412</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2008 Feb;82(4):1851-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18077720</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2012 Oct;86(20):11128-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22855488</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24043791</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Opin Mol Ther. 2000 Oct;2(5):555-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11249758</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Vaccine. 1990 Dec;8(6):605-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1708184</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Med. 2006 Dec;3(12):e525</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17194199</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2005 Feb 5;332(1):206-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15661153</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Viruses. 2010 Feb;2(2):413-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21994644</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Jul;79(13):8065-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15956552</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 Nov;74(22):10600-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11044104</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Feb;73(2):1535-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9882359</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3484-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19208801</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Jul;75(14):6676-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11413334</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Med. 2004 Apr;10(4):368-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15034574</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1988 Jun;62(6):1849-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3130492</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1990 Aug;177(2):646-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2164728</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Jan;79(2):884-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15613317</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Methods Mol Biol. 2008;454:255-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19057872</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Genet. 1998 Oct;20(2):123-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9771703</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2009 Sep 1;391(2):304-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19580983</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1994 Dec;68(12):8169-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7966608</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2006 Apr;80(7):3670-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16537637</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biotechniques. 1997 Dec;23(6):992-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9421622</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Mar;79(5):3097-106</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15709029</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11371-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8876142</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol Sin. 2010 Oct;25(5):361-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20960182</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1998 Feb 1;241(1):112-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9454722</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2010 Feb;84(4):2169-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19955314</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1992 Jul;189(1):88-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1318616</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Genomics. 2003 Jul;82(1):68-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12809677</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Rev Med Virol. 2003 Mar-Apr;13(2):111-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12627394</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2004 Jun;85(Pt 6):1717-1725</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15166457</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1992 Sep;190(1):522-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1529553</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2008 Apr;133(1):45-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17416434</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2006 Nov;80(21):10900-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16928748</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Virus Res. 1999;53:245-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10582102</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Jun;79(11):7095-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890949</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1990 Jun;64(6):3108-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2159565</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Viral Hepat. 2007 Nov;14 Suppl 1:64-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17958645</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1991 Feb;180(2):625-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1989387</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Espagne</li>
</country>
<region><li>Communauté de Madrid</li>
</region>
<settlement><li>Madrid</li>
</settlement>
<orgName><li>Université autonome de Madrid</li>
</orgName>
</list>
<tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name>
</region>
<name sortKey="Becares, Martina" sort="Becares, Martina" uniqKey="Becares M" first="Martina" last="Becares">Martina Becares</name>
<name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Marquez-Jurado">Silvia Marquez-Jurado</name>
<name sortKey="Morales, Lucia" sort="Morales, Lucia" uniqKey="Morales L" first="Lucia" last="Morales">Lucia Morales</name>
<name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name>
<name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001026 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 001026 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:24930446 |texte= Coronavirus reverse genetic systems: infectious clones and replicons. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:24930446" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |