A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers.
Identifieur interne : 000E70 ( PubMed/Checkpoint ); précédent : 000E69; suivant : 000E71A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers.
Auteurs : Yan Du [États-Unis] ; Randall A. Hughes [États-Unis] ; Sanchita Bhadra [États-Unis] ; Yu Sherry Jiang [États-Unis] ; Andrew D. Ellington [États-Unis] ; Bingling Li [États-Unis]Source :
- Scientific reports [ 2045-2322 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Glucose.
- génétique : Ebolavirus, Virus du SRAS.
- Ebolavirus, Protéines bactériennes, Techniques d'amplification d'acides nucléiques, Virus du SRAS, beta-Fructofuranosidase.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Glucose.
- chemical , chemistry : Bacterial Proteins, beta-Fructofuranosidase.
- chemistry : Ebolavirus, SARS Virus.
- genetics : Ebolavirus, SARS Virus.
- Nucleic Acid Amplification Techniques.
Abstract
Strand exchange nucleic acid circuitry can be used to transduce isothermal nucleic acid amplification products into signals that can be readable on an off-the-shelf glucometer. Loop-mediated isothermal amplification (LAMP) is limited by the accumulation of non-specific products, but nucleic acid circuitry can be used to probe and distinguish specific amplicons. By combining this high temperature isothermal amplification method with a thermostable invertase, we can directly transduce Middle-East respiratory syndrome coronavirus and Zaire Ebolavirus templates into glucose signals, with a sensitivity as low as 20-100 copies/μl, equating to atto-molar (or low zepto-mole). Virus from cell lysates and synthetic templates could be readily amplified and detected even in sputum or saliva. An OR gate that coordinately triggered on viral amplicons further guaranteed fail-safe virus detection. The method describes has potential for accelerating point-of-care applications, in that biological samples could be applied to a transducer that would then directly interface with an off-the-shelf, approved medical device.
DOI: 10.1038/srep11039
PubMed: 26050646
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:26050646Le document en format XML
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Ellington</name><affiliation wicri:level="4"><nlm:affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712</wicri:regionArea><placeName><region type="state">Texas</region><settlement type="city">Austin (Texas)</settlement></placeName><orgName type="university">Université du Texas à Austin</orgName></affiliation></author><author><name sortKey="Li, Bingling" sort="Li, Bingling" uniqKey="Li B" first="Bingling" last="Li">Bingling Li</name><affiliation wicri:level="4"><nlm:affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712</wicri:regionArea><placeName><region type="state">Texas</region><settlement type="city">Austin (Texas)</settlement></placeName><orgName type="university">Université du Texas à Austin</orgName></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (chemistry)</term><term>Ebolavirus (chemistry)</term><term>Ebolavirus (genetics)</term><term>Glucose (analysis)</term><term>Nucleic Acid Amplification Techniques</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>beta-Fructofuranosidase (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ebolavirus ()</term><term>Ebolavirus (génétique)</term><term>Glucose (analyse)</term><term>Protéines bactériennes ()</term><term>Techniques d'amplification d'acides nucléiques</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>beta-Fructofuranosidase ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bacterial Proteins</term><term>beta-Fructofuranosidase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucose</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ebolavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ebolavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ebolavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Ebolavirus</term><term>Protéines bactériennes</term><term>Techniques d'amplification d'acides nucléiques</term><term>Virus du SRAS</term><term>beta-Fructofuranosidase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Strand exchange nucleic acid circuitry can be used to transduce isothermal nucleic acid amplification products into signals that can be readable on an off-the-shelf glucometer. Loop-mediated isothermal amplification (LAMP) is limited by the accumulation of non-specific products, but nucleic acid circuitry can be used to probe and distinguish specific amplicons. By combining this high temperature isothermal amplification method with a thermostable invertase, we can directly transduce Middle-East respiratory syndrome coronavirus and Zaire Ebolavirus templates into glucose signals, with a sensitivity as low as 20-100 copies/μl, equating to atto-molar (or low zepto-mole). Virus from cell lysates and synthetic templates could be readily amplified and detected even in sputum or saliva. An OR gate that coordinately triggered on viral amplicons further guaranteed fail-safe virus detection. The method describes has potential for accelerating point-of-care applications, in that biological samples could be applied to a transducer that would then directly interface with an off-the-shelf, approved medical device. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26050646</PMID><DateCompleted><Year>2016</Year><Month>06</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><PubDate><Year>2015</Year><Month>Jun</Month><Day>08</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers.</ArticleTitle><Pagination><MedlinePgn>11039</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep11039</ELocationID><Abstract><AbstractText>Strand exchange nucleic acid circuitry can be used to transduce isothermal nucleic acid amplification products into signals that can be readable on an off-the-shelf glucometer. Loop-mediated isothermal amplification (LAMP) is limited by the accumulation of non-specific products, but nucleic acid circuitry can be used to probe and distinguish specific amplicons. By combining this high temperature isothermal amplification method with a thermostable invertase, we can directly transduce Middle-East respiratory syndrome coronavirus and Zaire Ebolavirus templates into glucose signals, with a sensitivity as low as 20-100 copies/μl, equating to atto-molar (or low zepto-mole). Virus from cell lysates and synthetic templates could be readily amplified and detected even in sputum or saliva. An OR gate that coordinately triggered on viral amplicons further guaranteed fail-safe virus detection. The method describes has potential for accelerating point-of-care applications, in that biological samples could be applied to a transducer that would then directly interface with an off-the-shelf, approved medical device. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hughes</LastName><ForeName>Randall A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Applied Research Laboratories, The University of Texas at Austin, Austin, TX 78758, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bhadra</LastName><ForeName>Sanchita</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Yu Sherry</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Department of Chemsitry, The University of Texas at Austin, Austin, TX 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ellington</LastName><ForeName>Andrew D</ForeName><Initials>AD</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Bingling</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX 78712, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>U54 EB015403</GrantID><Acronym>EB</Acronym><Agency>NIBIB NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>5U54EB015403-4500001623</GrantID><Acronym>EB</Acronym><Agency>NIBIB NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.26</RegistryNumber><NameOfSubstance UI="D043324">beta-Fructofuranosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029043" MajorTopicYN="N">Ebolavirus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="Y">Nucleic Acid Amplification 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IdType="pubmed">23842446</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region><settlement><li>Austin (Texas)</li></settlement><orgName><li>Université du Texas à Austin</li></orgName></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Du, Yan" sort="Du, Yan" uniqKey="Du Y" first="Yan" last="Du">Yan Du</name></region><name sortKey="Bhadra, Sanchita" sort="Bhadra, Sanchita" uniqKey="Bhadra S" first="Sanchita" last="Bhadra">Sanchita Bhadra</name><name sortKey="Ellington, Andrew D" sort="Ellington, Andrew D" uniqKey="Ellington A" first="Andrew D" last="Ellington">Andrew D. Ellington</name><name sortKey="Hughes, Randall A" sort="Hughes, Randall A" uniqKey="Hughes R" first="Randall A" last="Hughes">Randall A. Hughes</name><name sortKey="Jiang, Yu Sherry" sort="Jiang, Yu Sherry" uniqKey="Jiang Y" first="Yu Sherry" last="Jiang">Yu Sherry Jiang</name><name sortKey="Li, Bingling" sort="Li, Bingling" uniqKey="Li B" first="Bingling" last="Li">Bingling Li</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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