Alisporivir inhibits MERS- and SARS-coronavirus replication in cell culture, but not SARS-coronavirus infection in a mouse model.
Identifieur interne : 000B58 ( PubMed/Checkpoint ); précédent : 000B57; suivant : 000B59Alisporivir inhibits MERS- and SARS-coronavirus replication in cell culture, but not SARS-coronavirus infection in a mouse model.
Auteurs : Adriaan H. De Wilde [Pays-Bas] ; Darryl Falzarano [États-Unis] ; Jessika C. Zevenhoven-Dobbe [Pays-Bas] ; Corrine Beugeling [Pays-Bas] ; Craig Fett [États-Unis] ; Cynthia Martellaro [États-Unis] ; Clara C. Posthuma [Pays-Bas] ; Heinz Feldmann [États-Unis] ; Stanley Perlman [États-Unis] ; Eric J. Snijder [Pays-Bas]Source :
- Virus research [ 1872-7492 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (pharmacologie), Cellules cultivées, Ciclosporine (pharmacologie), Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Effet cytopathogène viral (), Humains, Infections à coronavirus (traitement médicamenteux), Infections à coronavirus (virologie), Lignée cellulaire, Modèles animaux de maladie humaine, Relation dose-effet des médicaments, Réplication virale (), Souris, Syndrome respiratoire aigu sévère (traitement médicamenteux), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- pharmacologie : Antiviraux, Ciclosporine.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- traitement médicamenteux : Infections à coronavirus, Syndrome respiratoire aigu sévère.
- virologie : Infections à coronavirus, Syndrome respiratoire aigu sévère.
- Animaux, Cellules cultivées, Coronavirus du syndrome respiratoire du Moyen-Orient, Effet cytopathogène viral, Humains, Lignée cellulaire, Modèles animaux de maladie humaine, Relation dose-effet des médicaments, Réplication virale, Souris, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Cell Line, Cells, Cultured, Coronavirus Infections (drug therapy), Coronavirus Infections (virology), Cyclosporine (pharmacology), Cytopathogenic Effect, Viral (drug effects), Disease Models, Animal, Dose-Response Relationship, Drug, Humans, Mice, Middle East Respiratory Syndrome Coronavirus (drug effects), Middle East Respiratory Syndrome Coronavirus (physiology), SARS Virus (drug effects), SARS Virus (physiology), Severe Acute Respiratory Syndrome (drug therapy), Severe Acute Respiratory Syndrome (virology), Virus Replication (drug effects).
- MESH :
- chemical , pharmacology : Antiviral Agents, Cyclosporine.
- drug effects : Cytopathogenic Effect, Viral, Middle East Respiratory Syndrome Coronavirus, SARS Virus, Virus Replication.
- drug therapy : Coronavirus Infections, Severe Acute Respiratory Syndrome.
- physiology : Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- virology : Coronavirus Infections, Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Cells, Cultured, Disease Models, Animal, Dose-Response Relationship, Drug, Humans, Mice.
Abstract
Currently, there is no registered treatment for infections with emerging zoonotic coronaviruses like SARS- and MERS-coronavirus. We here report that in cultured cells low-micromolar concentrations of alisporivir, a non-immunosuppressive cyclosporin A-analog, inhibit the replication of four different coronaviruses, including MERS- and SARS-coronavirus. Ribavirin was found to further potentiate the antiviral effect of alisporivir in these cell culture-based infection models, but this combination treatment was unable to improve the outcome of SARS-CoV infection in a mouse model. Nevertheless, our data provide a basis to further explore the potential of Cyp inhibitors as host-directed, broad-spectrum inhibitors of coronavirus replication.
DOI: 10.1016/j.virusres.2016.11.011
PubMed: 27840112
Affiliations:
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pubmed:27840112Le document en format XML
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<front><div type="abstract" xml:lang="en">Currently, there is no registered treatment for infections with emerging zoonotic coronaviruses like SARS- and MERS-coronavirus. We here report that in cultured cells low-micromolar concentrations of alisporivir, a non-immunosuppressive cyclosporin A-analog, inhibit the replication of four different coronaviruses, including MERS- and SARS-coronavirus. Ribavirin was found to further potentiate the antiviral effect of alisporivir in these cell culture-based infection models, but this combination treatment was unable to improve the outcome of SARS-CoV infection in a mouse model. Nevertheless, our data provide a basis to further explore the potential of Cyp inhibitors as host-directed, broad-spectrum inhibitors of coronavirus replication.</div>
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<Abstract><AbstractText>Currently, there is no registered treatment for infections with emerging zoonotic coronaviruses like SARS- and MERS-coronavirus. We here report that in cultured cells low-micromolar concentrations of alisporivir, a non-immunosuppressive cyclosporin A-analog, inhibit the replication of four different coronaviruses, including MERS- and SARS-coronavirus. Ribavirin was found to further potentiate the antiviral effect of alisporivir in these cell culture-based infection models, but this combination treatment was unable to improve the outcome of SARS-CoV infection in a mouse model. Nevertheless, our data provide a basis to further explore the potential of Cyp inhibitors as host-directed, broad-spectrum inhibitors of coronavirus replication.</AbstractText>
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