Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.
Identifieur interne : 000322 ( PubMed/Checkpoint ); précédent : 000321; suivant : 000323Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.
Auteurs : Yang Pan [République populaire de Chine] ; Luyao Long [République populaire de Chine] ; Daitao Zhang [République populaire de Chine] ; Tingting Yan [République populaire de Chine] ; Shujuan Cui [République populaire de Chine] ; Peng Yang [République populaire de Chine] ; Quanyi Wang [République populaire de Chine] ; Simei Ren [République populaire de Chine]Source :
- Clinical chemistry [ 1530-8561 ] ; 2020.
Abstract
Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests.
DOI: 10.1093/clinchem/hvaa091
PubMed: 32246822
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Daitao" sort="Zhang, Daitao" uniqKey="Zhang D" first="Daitao" last="Zhang">Daitao Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Tingting" sort="Yan, Tingting" uniqKey="Yan T" first="Tingting" last="Yan">Tingting Yan</name><affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cui, Shujuan" sort="Cui, Shujuan" uniqKey="Cui S" first="Shujuan" last="Cui">Shujuan Cui</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Quanyi" sort="Wang, Quanyi" uniqKey="Wang Q" first="Quanyi" last="Wang">Quanyi Wang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Simei" sort="Ren, Simei" uniqKey="Ren S" first="Simei" last="Ren">Simei Ren</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32246822</idno><idno type="pmid">32246822</idno><idno type="doi">10.1093/clinchem/hvaa091</idno><idno type="wicri:Area/PubMed/Corpus">000356</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000356</idno><idno type="wicri:Area/PubMed/Curation">000356</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000356</idno><idno type="wicri:Area/PubMed/Checkpoint">000322</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000322</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.</title><author><name sortKey="Pan, Yang" sort="Pan, Yang" uniqKey="Pan Y" first="Yang" last="Pan">Yang Pan</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Long, Luyao" sort="Long, Luyao" uniqKey="Long L" first="Luyao" last="Long">Luyao Long</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Daitao" sort="Zhang, Daitao" uniqKey="Zhang D" first="Daitao" last="Zhang">Daitao Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Tingting" sort="Yan, Tingting" uniqKey="Yan T" first="Tingting" last="Yan">Tingting Yan</name><affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cui, Shujuan" sort="Cui, Shujuan" uniqKey="Cui S" first="Shujuan" last="Cui">Shujuan Cui</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Quanyi" sort="Wang, Quanyi" uniqKey="Wang Q" first="Quanyi" last="Wang">Quanyi Wang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Simei" sort="Ren, Simei" uniqKey="Ren S" first="Simei" last="Ren">Simei Ren</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author></analytic><series><title level="j">Clinical chemistry</title><idno type="eISSN">1530-8561</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32246822</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1530-8561</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Apr</Month><Day>04</Day></PubDate></JournalIssue><Title>Clinical chemistry</Title><ISOAbbreviation>Clin. Chem.</ISOAbbreviation></Journal><ArticleTitle>Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">hvaa091</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/clinchem/hvaa091</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We investigated whether thermal inactivation could affect the results of viral NAT. We examined the effects of thermal inactivation on the quantitative RT-PCR results of SARS-CoV-2 particularly with regard to the rates of false-negative results for specimens carrying low viral loads. We additionally investigated the effects of different specimen types, sample preservation times and a chemical inactivation approach on NAT.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Our work showed increased Ct values in specimens from diagnosed COVID-19 patients in RT-PCR tests after thermal incubation. Moreover, about half of the weak-positive samples (7 of 15 samples, 46.7%) were RT-PCR negative after heat inactivation in at least one parallel testing. The use of guanidinium-based lysis for preservation of these specimens had a smaller impact on RT-PCR results with fewer false negatives (2 of 15 samples, 13.3%) and significantly less increase in Ct values than heat inactivation.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Thermal inactivation adversely affected the efficiency of RT-PCR for SARS-CoV-2 detection. Given the limited applicability associated with chemical inactivators, other approaches to ensure the overall protection of laboratory personnel need consideration.</AbstractText><CopyrightInformation>© 2020 American Association for Clinical Chemistry.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Centre for Preventive Medicine of Beijing, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Public Health, Capital Medical University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Luyao</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Graduate School, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Daitao</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Centre for Preventive Medicine of Beijing, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Public Health, Capital Medical University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Tingting</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cui</LastName><ForeName>Shujuan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Centre for Preventive Medicine of Beijing, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Public Health, Capital Medical University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Centre for Preventive Medicine of Beijing, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Public Health, Capital Medical University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Quanyi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Centre for Preventive Medicine of Beijing, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Public Health, Capital Medical University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Simei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Graduate School, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Clin Chem</MedlineTA><NlmUniqueID>9421549</NlmUniqueID><ISSNLinking>0009-9147</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">False negative</Keyword><Keyword MajorTopicYN="N">Nucleic Acid Test</Keyword><Keyword MajorTopicYN="N">RT-PCR</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">Thermal Inactivation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32246822</ArticleId><ArticleId IdType="pii">5815979</ArticleId><ArticleId IdType="doi">10.1093/clinchem/hvaa091</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Pan, Yang" sort="Pan, Yang" uniqKey="Pan Y" first="Yang" last="Pan">Yang Pan</name></noRegion><name sortKey="Cui, Shujuan" sort="Cui, Shujuan" uniqKey="Cui S" first="Shujuan" last="Cui">Shujuan Cui</name><name sortKey="Long, Luyao" sort="Long, Luyao" uniqKey="Long L" first="Luyao" last="Long">Luyao Long</name><name sortKey="Ren, Simei" sort="Ren, Simei" uniqKey="Ren S" first="Simei" last="Ren">Simei Ren</name><name sortKey="Wang, Quanyi" sort="Wang, Quanyi" uniqKey="Wang Q" first="Quanyi" last="Wang">Quanyi Wang</name><name sortKey="Yan, Tingting" sort="Yan, Tingting" uniqKey="Yan T" first="Tingting" last="Yan">Tingting Yan</name><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><name sortKey="Zhang, Daitao" sort="Zhang, Daitao" uniqKey="Zhang D" first="Daitao" last="Zhang">Daitao Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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