SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Identifieur interne : 000222 ( PubMed/Checkpoint ); précédent : 000221; suivant : 000223SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Auteurs : Soeren Lukassen [Allemagne] ; Robert Lorenz Chua [Allemagne] ; Timo Trefzer [Allemagne] ; Nicolas C. Kahn [Allemagne] ; Marc A. Schneider [Allemagne] ; Thomas Muley [Allemagne] ; Hauke Winter [Allemagne] ; Michael Meister [Allemagne] ; Carmen Veith [Allemagne] ; Agnes W. Boots [Pays-Bas] ; Bianca P. Hennig [Allemagne] ; Michael Kreuter [Allemagne] ; Christian Conrad [Allemagne] ; Roland Eils [Allemagne]Source :
- The EMBO journal [ 1460-2075 ] ; 2020.
Abstract
The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.
DOI: 10.15252/embj.20105114
PubMed: 32246845
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:32246845Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.</title><author><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht</wicri:regionArea><wicri:noRegion>Maastricht</wicri:noRegion></affiliation></author><author><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32246845</idno><idno type="pmid">32246845</idno><idno type="doi">10.15252/embj.20105114</idno><idno type="wicri:Area/PubMed/Corpus">000355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000355</idno><idno type="wicri:Area/PubMed/Curation">000355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000355</idno><idno type="wicri:Area/PubMed/Checkpoint">000222</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000222</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.</title><author><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht</wicri:regionArea><wicri:noRegion>Maastricht</wicri:noRegion></affiliation></author><author><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">The EMBO journal</title><idno type="eISSN">1460-2075</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32246845</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2075</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Apr</Month><Day>04</Day></PubDate></JournalIssue><Title>The EMBO journal</Title><ISOAbbreviation>EMBO J.</ISOAbbreviation></Journal><ArticleTitle>SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.</ArticleTitle><Pagination><MedlinePgn>e105114</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.15252/embj.20105114</ELocationID><Abstract><AbstractText>The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.</AbstractText><CopyrightInformation>© 2020 The Authors. Published under the terms of the CC BY 4.0 license.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Lukassen</LastName><ForeName>Soeren</ForeName><Initials>S</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-7045-6327</Identifier><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Chua</LastName><ForeName>Robert Lorenz</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Trefzer</LastName><ForeName>Timo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Kahn</LastName><ForeName>Nicolas C</ForeName><Initials>NC</Initials><AffiliationInfo><Affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Schneider</LastName><ForeName>Marc A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Translational Research Unit, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muley</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Translational Research Unit, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Winter</LastName><ForeName>Hauke</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Thoracic Surgery, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meister</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Translational Research Unit, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Veith</LastName><ForeName>Carmen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boots</LastName><ForeName>Agnes W</ForeName><Initials>AW</Initials><AffiliationInfo><Affiliation>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hennig</LastName><ForeName>Bianca P</ForeName><Initials>BP</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Kreuter</LastName><ForeName>Michael</ForeName><Initials>M</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-4402-2159</Identifier><AffiliationInfo><Affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Conrad</LastName><ForeName>Christian</ForeName><Initials>C</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-7036-342X</Identifier><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Eils</LastName><ForeName>Roland</ForeName><Initials>R</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-0034-4036</Identifier><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Digital Health, Berlin Institute of Health (BIH), Berlin, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Health Data Science Unit, Heidelberg University Hospital and BioQuant, Heidelberg, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>874710</GrantID><Agency>EC|Horizon 2020 Framework Programme (H2020)</Agency><Country></Country></Grant><Grant><GrantID>82DZL00402</GrantID><Agency>German Center for Lung Research (DLZ)</Agency><Country></Country></Grant><Grant><Agency>European Respiratory Society (STRTF - 201804-00377) - fellowship CV</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>EMBO J</MedlineTA><NlmUniqueID>8208664</NlmUniqueID><ISSNLinking>0261-4189</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">FURIN
</Keyword><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">Human Cell Atlas</Keyword><Keyword MajorTopicYN="N">epithelial differentiation</Keyword><Keyword MajorTopicYN="N">respiratory tract</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32246845</ArticleId><ArticleId IdType="doi">10.15252/embj.20105114</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Bakken TE, Hodge RD, Miller JA, Yao Z, Nguyen TN, Aevermann B, Barkan E, Bertagnolli D, Casper T, Dee N et al (2018) Single-nucleus and single-cell transcriptomes compared in matched cortical cell types. PLoS One 13: e0209648</Citation></Reference><Reference><Citation>Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc 57: 289-300</Citation></Reference><Reference><Citation>Bi Q, Wu Y, Mei S, Ye C, Zou X, Zhang Z, Liu X, Wei L, Truelove SA, Zhang T et al (2020) Epidemiology and transmission of COVID-19 in shenzhen china: analysis of 391 cases and 1,286 of their close contacts. medRxiv https://doi.org/10.1101/2020.03.03.20028423 [PREPRINT]</Citation></Reference><Reference><Citation>Brussow H (2020) The novel coronavirus - a snapshot of current knowledge. Microb Biotechnol 13: 607-612</Citation></Reference><Reference><Citation>Burkard C, Verheije MH, Wicht O, van Kasteren SI, van Kuppeveld FJ, Haagmans BL, Pelkmans L, Rottier PJ, Bosch BJ, de Haan CA (2014) Coronavirus cell entry occurs through the endo-/lysosomal pathway in a proteolysis-dependent manner. PLoS Pathog 10: e1004502</Citation></Reference><Reference><Citation>Cao J, Spielmann M, Qiu X, Huang X, Ibrahim DM, Hill AJ, Zhang F, Mundlos S, Christiansen L, Steemers FJ et al (2019) The single-cell transcriptional landscape of mammalian organogenesis. Nature 566: 496-502</Citation></Reference><Reference><Citation>Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, Xing F, Liu J, Yip CC, Poon RW et al (2020) A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet 395: 514-523</Citation></Reference><Reference><Citation>Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, Qiu Y, Wang J, Liu Y, Wei Y et al (2020) Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet 395: 507-513</Citation></Reference><Reference><Citation>Coutard B, Valle C, de Lamballerie X, Canard B, Seidah NG, Decroly E (2020) The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. Antiviral Res 176: 104742</Citation></Reference><Reference><Citation>Dumm RE, Fiege JK, Waring BM, Kuo CT, Langlois RA, Heaton NS (2019) Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function. Nat Commun 10: 779</Citation></Reference><Reference><Citation>Dunn OJ (1964) Multiple comparisons using rank sums. Technometrics 6: 241-252</Citation></Reference><Reference><Citation>Gallagher TM, Buchmeier MJ (2001) Coronavirus spike proteins in viral entry and pathogenesis. Virology 279: 371-374</Citation></Reference><Reference><Citation>Gorbalenya AE, Baker SC, Baric RS, de Groot RJ, Drosten C, Gulyaeva AA, Haagmans BL, Lauber C, Leontovich AM, Neuman BW et al (2020) The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nat Microbiol 5: 536-544</Citation></Reference><Reference><Citation>Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, Liu L, Shan H, Lei CL, Hui DSC et al (2020) Clinical characteristics of Coronavirus Disease 2019 in China. N Engl J Med https://doi.org/10.1056/NEJMoa2002032</Citation></Reference><Reference><Citation>Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G, van Goor H (2004) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol 203: 631-637</Citation></Reference><Reference><Citation>Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens TS, Herrler G, Wu NH, Nitsche A et al (2020) SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell https://doi.org/10.1016/j.cell.2020.02.052</Citation></Reference><Reference><Citation>Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, Zhang L, Fan G, Xu J, Gu X et al (2020) Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 395: 497-506</Citation></Reference><Reference><Citation>Jia HP, Look DC, Shi L, Hickey M, Pewe L, Netland J, Farzan M, Wohlford-Lenane C, Perlman S, McCray PB Jr (2005) ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epithelia. J Virol 79: 14614-14621</Citation></Reference><Reference><Citation>Kahn N, Kuner R, Eberhardt R, Meister M, Muley T, Winteroll S, Schnabel PA, Ishizaka A, Herth FJ, Poustka A et al (2009) Gene expression analysis of endobronchial epithelial lining fluid in the evaluation of indeterminate pulmonary nodules. J Thorac Cardiovasc Surg 138: 474-479</Citation></Reference><Reference><Citation>Kawase M, Shirato K, van der Hoek L, Taguchi F, Matsuyama S (2012) Simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry. J Virol 86: 6537-6545</Citation></Reference><Reference><Citation>Kido H, Okumura Y, Takahashi E, Pan HY, Wang S, Yao D, Yao M, Chida J, Yano M (2012) Role of host cellular proteases in the pathogenesis of influenza and influenza-induced multiple organ failure. Biochim Biophys Acta 1824: 186-194</Citation></Reference><Reference><Citation>Kirchdoerfer RN, Cottrell CA, Wang N, Pallesen J, Yassine HM, Turner HL, Corbett KS, Graham BS, McLellan JS, Ward AB (2016) Pre-fusion structure of a human coronavirus spike protein. Nature 531: 118-121</Citation></Reference><Reference><Citation>Kruskal WH, Wallis WA (1952) Use of ranks in one-criterion variance analysis. J Am Stat Assoc 47: 583-621</Citation></Reference><Reference><Citation>Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC et al (2003) Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 426: 450-454</Citation></Reference><Reference><Citation>Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N et al (2020) Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395: 565-574</Citation></Reference><Reference><Citation>Luecken MD, Theis FJ (2019) Current best practices in single-cell RNA-seq analysis: a tutorial. Mol Syst Biol 15: e8746</Citation></Reference><Reference><Citation>Matsuyama S, Ujike M, Morikawa S, Tashiro M, Taguchi F (2005) Protease-mediated enhancement of severe acute respiratory syndrome coronavirus infection. Proc Natl Acad Sci USA 102: 12543-12547</Citation></Reference><Reference><Citation>Millet JK, Whittaker GR (2014) Host cell entry of Middle East respiratory syndrome coronavirus after two-step, furin-mediated activation of the spike protein. Proc Natl Acad Sci USA 111: 15214-15219</Citation></Reference><Reference><Citation>Muley TR, Herth FJ, Schnabel PA, Dienemann H, Meister M (2012) From tissue to molecular phenotyping: pre-analytical requirements heidelberg experience. Transl Lung Cancer Res 1: 111-121</Citation></Reference><Reference><Citation>Qiu X, Mao Q, Tang Y, Wang L, Chawla R, Pliner HA, Trapnell C (2017) Reversed graph embedding resolves complex single-cell trajectories. Nat Methods 14: 979-982</Citation></Reference><Reference><Citation>Ren X, Glende J, Al-Falah M, de Vries V, Schwegmann-Wessels C, Qu X, Tan L, Tschernig T, Deng H, Naim HY et al (2006) Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus. J Gen Virol 87: 1691-1695</Citation></Reference><Reference><Citation>Simmons G, Reeves JD, Rennekamp AJ, Amberg SM, Piefer AJ, Bates P (2004) Characterization of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike glycoprotein-mediated viral entry. Proc Natl Acad Sci USA 101: 4240-4245</Citation></Reference><Reference><Citation>Simmons G, Zmora P, Gierer S, Heurich A, Pohlmann S (2013) Proteolytic activation of the SARS-coronavirus spike protein: cutting enzymes at the cutting edge of antiviral research. Antiviral Res 100: 605-614</Citation></Reference><Reference><Citation>Sims AC, Burkett SE, Yount B, Pickles RJ (2008) SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium. Virus Res 133: 33-44</Citation></Reference><Reference><Citation>Sungnak W, Huang N, Bécavin C, Berg M, Network HLB (2020) SARS-CoV-2 entry genes are most highly expressed in nasal goblet and ciliated cells within human airways. arXiv arXiv:2003.06122 [PREPRINT]</Citation></Reference><Reference><Citation>Tosti L, Hang Y, Trefzer T, Steiger K, Ten FW, Lukassen S, Ballke S, Kuehl AA, Spieckermann S, Bottino R et al (2019) Single nucleus RNA sequencing maps acinar cell states in a human pancreas cell atlas. bioRxiv https://doi.org/10.1101/733964 [PREPRINT]</Citation></Reference><Reference><Citation>Van den Broeke C, Jacob T, Favoreel HW (2014) Rho'ing in and out of cells: viral interactions with Rho GTPase signaling. Small GTPases 5: e28318</Citation></Reference><Reference><Citation>Vidricaire G, Denault JB, Leduc R (1993) Characterization of a secreted form of human furin endoprotease. Biochem Biophys Res Commun 195: 1011-1018</Citation></Reference><Reference><Citation>Walls AC, Tortorici MA, Bosch BJ, Frenz B, Rottier PJM, DiMaio F, Rey FA, Veesler D (2016) Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer. Nature 531: 114-117</Citation></Reference><Reference><Citation>Walls AC, Park Y-J, Tortorici MA, Wall A, McGuire AT, Veesler D (2020) Structure, function and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell https://doi.org/10.1016/j.cell.2020.02.058</Citation></Reference><Reference><Citation>Wang H, Yang P, Liu K, Guo F, Zhang Y, Zhang G, Jiang C (2008) SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway. Cell Res 18: 290-301</Citation></Reference><Reference><Citation>Wang C, Horby PW, Hayden FG, Gao GF (2020a) A novel coronavirus outbreak of global health concern. Lancet 395: 470-473</Citation></Reference><Reference><Citation>Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J, Wang B, Xiang H, Cheng Z, Xiong Y et al (2020b) Clinical characteristics of 138 hospitalized patients with 2019 novel Coronavirus-infected pneumonia in Wuhan, China. JAMA 323: 1061-1069</Citation></Reference><Reference><Citation>White JM, Whittaker GR (2016) Fusion of enveloped viruses in endosomes. Traffic 17: 593-614</Citation></Reference><Reference><Citation>WHO (2016) Middle East respiratory syndrome coronavirus (MERS-CoV) - Saudi Arabia. www.hoint/csr/don/26-april-2016-mers-saudi-arabia/en/</Citation></Reference><Reference><Citation>de Wit E, van Doremalen N, Falzarano D, Munster VJ (2016) SARS and MERS: recent insights into emerging coronaviruses. Nat Rev Microbiol 14: 523-534</Citation></Reference><Reference><Citation>Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh CL, Abiona O, Graham BS, McLellan JS (2020) Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 367: 1260-1263</Citation></Reference><Reference><Citation>Wu Z, McGoogan JM (2020) Characteristics of and important lessons from the coronavirus disease 2019 (COVID-19) outbreak in China: summary of a Report of 72314 cases from the Chinese Center for disease control and prevention. JAMA https://doi.org/10.1001/jama.2020.2648</Citation></Reference><Reference><Citation>Wu A, Peng Y, Huang B, Ding X, Wang X, Niu P, Meng J, Zhu Z, Zhang Z, Wang J et al (2020a) Genome composition and divergence of the novel coronavirus (2019-nCoV) originating in China. Cell Host Microbe 27: 325-328</Citation></Reference><Reference><Citation>Wu C, Yang Y, Liu Y, Zhang P, Wang Y, Wang Q, Xu Y, Li M, Zheng M, Chen L et al (2020b) Furin, a potential therapeutic target for COVID-19. chinaRxiv https://doi.org/10.12074/202002.00062 [PREPRINT]</Citation></Reference><Reference><Citation>Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, Tao ZW, Tian JH, Pei YY et al (2020c) A new coronavirus associated with human respiratory disease in China. Nature 579: 265-269</Citation></Reference><Reference><Citation>Xu H, Zhong L, Deng J, Peng J, Dan H, Zeng X, Li T, Chen Q (2020) High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa. Int J Oral Sci 12: 8</Citation></Reference><Reference><Citation>Yan R, Zhang Y, Li Y, Xia L, Guo Y, Zhou Q (2020) Structural basis for the recognition of the SARS-CoV-2 by full-length human ACE2. Science 367: 1444-1448</Citation></Reference><Reference><Citation>Zhang JJ, Dong X, Cao YY, Yuan YD, Yang YB, Yan YQ, Akdis CA, Gao YD (2020a) Clinical characteristics of 140 patients infected with SARS-CoV-2 in Wuhan, China. Allergy https://doi.org/10.1111/all.14238</Citation></Reference><Reference><Citation>Zhang Y-Z, Koopmans M, Yuen KY (2020b) The novel coronavirus outbreak: what we know and what we don't. Cell 180: 1034-1036</Citation></Reference><Reference><Citation>Zhao Y, Zaho Z, Wand Y, Zhou Y, Ma Y, Zuo W (2020) Single-cell RNA expression profiling of ACE2, the putative receptor of Wuhan 2019-nCov. BioRxiv https://doi.org/10.1101/2020.01.26.919985 [PREPRINT]</Citation></Reference><Reference><Citation>Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi AH, Tanaseichuk O, Benner C, Chanda SK (2019) Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat Commun 10: 1523</Citation></Reference><Reference><Citation>Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL et al (2020) A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579: 270-273</Citation></Reference><Reference><Citation>Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R et al (2020) A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med 382: 727-733</Citation></Reference><Reference><Citation>Zou X, Chen K, Zou J, Han P, Hao J, Han Z (2020) The single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to Wuhan 2019-nCoV infection. Front Med https://doi.org/10.1007/s11684-020-0754-0</Citation></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country><region><li>Bade-Wurtemberg</li><li>Berlin</li><li>District de Karlsruhe</li></region><settlement><li>Berlin</li><li>Heidelberg</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name></region><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name></country><country name="Pays-Bas"><noRegion><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000222 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000222 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:32246845
|texte= SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:32246845" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |