Programmed ribosomal frameshifting in decoding the SARS-CoV genome
Identifieur interne : 001544 ( Pmc/Curation ); précédent : 001543; suivant : 001545Programmed ribosomal frameshifting in decoding the SARS-CoV genome
Auteurs : Pavel V. Baranov [États-Unis, Irlande (pays)] ; Clark M. Henderson [États-Unis] ; Christine B. Anderson [États-Unis] ; Raymond F. Gesteland [États-Unis] ; John F. Atkins [États-Unis, Irlande (pays)] ; Michael T. Howard [États-Unis]Source :
- Virology [ 0042-6822 ] ; 2005.
Abstract
Programmed ribosomal frameshifting is an essential mechanism used for the expression of
Url:
DOI: 10.1016/j.virol.2004.11.038
PubMed: 15680415
PubMed Central: 7111862
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PMC:7111862Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Programmed ribosomal frameshifting in decoding the SARS-CoV genome</title>
<author><name sortKey="Baranov, Pavel V" sort="Baranov, Pavel V" uniqKey="Baranov P" first="Pavel V." last="Baranov">Pavel V. Baranov</name>
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<series><title level="j">Virology</title>
<idno type="ISSN">0042-6822</idno>
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<front><div type="abstract" xml:lang="en"><p>Programmed ribosomal frameshifting is an essential mechanism used for the expression of <italic>orf1b</italic>
in coronaviruses. Comparative analysis of the frameshift region reveals a universal shift site U_UUA_AAC, followed by a predicted downstream RNA structure in the form of either a pseudoknot or kissing stem loops. Frameshifting in SARS-CoV has been characterized in cultured mammalian cells using a dual luciferase reporter system and mass spectrometry. Mutagenic analysis of the SARS-CoV shift site and mass spectrometry of an affinity tagged frameshift product confirmed tandem tRNA slippage on the sequence U_UUA_AAC. Analysis of the downstream pseudoknot stimulator of frameshifting in SARS-CoV shows that a proposed RNA secondary structure in loop II and two unpaired nucleotides at the stem I–stem II junction in SARS-CoV are important for frameshift stimulation. These results demonstrate key sequences required for efficient frameshifting, and the utility of mass spectrometry to study ribosomal frameshifting.</p>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virology</journal-id>
<journal-id journal-id-type="iso-abbrev">Virology</journal-id>
<journal-title-group><journal-title>Virology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0042-6822</issn>
<issn pub-type="epub">1096-0341</issn>
<publisher><publisher-name>Elsevier Inc.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15680415</article-id>
<article-id pub-id-type="pmc">7111862</article-id>
<article-id pub-id-type="publisher-id">S0042-6822(04)00813-X</article-id>
<article-id pub-id-type="doi">10.1016/j.virol.2004.11.038</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Programmed ribosomal frameshifting in decoding the SARS-CoV genome</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Baranov</surname>
<given-names>Pavel V.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="aff2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Henderson</surname>
<given-names>Clark M.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Anderson</surname>
<given-names>Christine B.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gesteland</surname>
<given-names>Raymond F.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Atkins</surname>
<given-names>John F.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="aff2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Howard</surname>
<given-names>Michael T.</given-names>
</name>
<email>mhoward@howard.genetics.utah.edu</email>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">*</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Department of Human Genetics, University of Utah, 15 N 2030 E, Room 7410, Salt Lake City, 84112-5330 UT, USA</aff>
<aff id="aff2"><label>b</label>
Bioscience Institute, University College Cork, Cork, Ireland</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Fax: +1 801 585 3910. <email>mhoward@howard.genetics.utah.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>5</day>
<month>1</month>
<year>2005</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>20</day>
<month>2</month>
<year>2005</year>
</pub-date>
<pub-date pub-type="epub"><day>5</day>
<month>1</month>
<year>2005</year>
</pub-date>
<volume>332</volume>
<issue>2</issue>
<fpage>498</fpage>
<lpage>510</lpage>
<history><date date-type="received"><day>4</day>
<month>8</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>7</day>
<month>9</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>30</day>
<month>11</month>
<year>2004</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2004 Elsevier Inc. All rights reserved.</copyright-statement>
<copyright-year>2004</copyright-year>
<copyright-holder>Elsevier Inc.</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract><p>Programmed ribosomal frameshifting is an essential mechanism used for the expression of <italic>orf1b</italic>
in coronaviruses. Comparative analysis of the frameshift region reveals a universal shift site U_UUA_AAC, followed by a predicted downstream RNA structure in the form of either a pseudoknot or kissing stem loops. Frameshifting in SARS-CoV has been characterized in cultured mammalian cells using a dual luciferase reporter system and mass spectrometry. Mutagenic analysis of the SARS-CoV shift site and mass spectrometry of an affinity tagged frameshift product confirmed tandem tRNA slippage on the sequence U_UUA_AAC. Analysis of the downstream pseudoknot stimulator of frameshifting in SARS-CoV shows that a proposed RNA secondary structure in loop II and two unpaired nucleotides at the stem I–stem II junction in SARS-CoV are important for frameshift stimulation. These results demonstrate key sequences required for efficient frameshifting, and the utility of mass spectrometry to study ribosomal frameshifting.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd>Pseudoknot</kwd>
<kwd>Recoding</kwd>
<kwd>Translation</kwd>
<kwd>Alternative decoding</kwd>
<kwd>SARS</kwd>
<kwd>Tandem slippage</kwd>
<kwd>Mass spectrometry</kwd>
<kwd>Frameshift</kwd>
<kwd>Coronavirus</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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