The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8+ T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848
Identifieur interne : 001535 ( Pmc/Curation ); précédent : 001534; suivant : 001536The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8+ T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848
Auteurs : Kai Zhao ; Hui Wang ; Changyou WuSource :
- Vaccine [ 0264-410X ] ; 2011.
Abstract
The induction of antigen specific memory CD8+ T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8+ T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8+ T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8+ T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8+ T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ+CD8+ T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8+ T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB+ and CD62L− and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.
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DOI: 10.1016/j.vaccine.2011.06.100
PubMed: 21745520
PubMed Central: 7115397
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup> T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</title><author><name sortKey="Zhao, Kai" sort="Zhao, Kai" uniqKey="Zhao K" first="Kai" last="Zhao">Kai Zhao</name></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21745520</idno><idno type="pmc">7115397</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115397</idno><idno type="RBID">PMC:7115397</idno><idno type="doi">10.1016/j.vaccine.2011.06.100</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">001535</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001535</idno><idno type="wicri:Area/Pmc/Curation">001535</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001535</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup> T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</title><author><name sortKey="Zhao, Kai" sort="Zhao, Kai" uniqKey="Zhao K" first="Kai" last="Zhao">Kai Zhao</name></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The induction of antigen specific memory CD8<sup>+</sup> T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8<sup>+</sup> T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8<sup>+</sup> T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8<sup>+</sup> T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8<sup>+</sup> T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ<sup>+</sup>CD8<sup>+</sup> T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8<sup>+</sup> T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB<sup>+</sup> and CD62L<sup>−</sup> and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name></author><author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S. 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Basta</name></author><author><name sortKey="Busch, D H" uniqKey="Busch D">D.H. Busch</name></author><author><name sortKey="Gander, B" uniqKey="Gander B">B. Gander</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salerno Goncalves, R" uniqKey="Salerno Goncalves R">R. Salerno-Goncalves</name></author><author><name sortKey="Sztein, M B" uniqKey="Sztein M">M.B. Sztein</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id><journal-id journal-id-type="iso-abbrev">Vaccine</journal-id><journal-title-group><journal-title>Vaccine</journal-title></journal-title-group><issn pub-type="ppub">0264-410X</issn><issn pub-type="epub">1873-2518</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21745520</article-id><article-id pub-id-type="pmc">7115397</article-id><article-id pub-id-type="publisher-id">S0264-410X(11)00994-7</article-id><article-id pub-id-type="doi">10.1016/j.vaccine.2011.06.100</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup> T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Kai</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hui</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Changyou</given-names></name><email>changyou_wu@yahoo.com</email><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff>Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, Guangdong Province 510080, People’ s Republic of China</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author. Tel.: +86 20 87332448; fax: +86 20 87332448. <email>changyou_wu@yahoo.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>13</day><month>7</month><year>2011</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>2</day><month>9</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>13</day><month>7</month><year>2011</year></pub-date><volume>29</volume><issue>38</issue><fpage>6670</fpage><lpage>6678</lpage><history><date date-type="received"><day>6</day><month>1</month><year>2011</year></date><date date-type="rev-recd"><day>13</day><month>6</month><year>2011</year></date><date date-type="accepted"><day>25</day><month>6</month><year>2011</year></date></history><permissions><copyright-statement>Copyright © 2011 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2011</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>The induction of antigen specific memory CD8<sup>+</sup> T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8<sup>+</sup> T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8<sup>+</sup> T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8<sup>+</sup> T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8<sup>+</sup> T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ<sup>+</sup>CD8<sup>+</sup> T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8<sup>+</sup> T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB<sup>+</sup> and CD62L<sup>−</sup> and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.</p></abstract><kwd-group><title>Abbreviations</title><kwd>SARS, severe acute respiratory syndrome</kwd><kwd>SARS-CoV, SARS-associated coronavirus</kwd><kwd>CpG ODN, CpG oligodeoxynucleotides</kwd><kwd>TLR, toll-like receptor</kwd></kwd-group><kwd-group><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Epitope</kwd><kwd>Adjuvant</kwd><kwd>T cell</kwd><kwd>Vaccine</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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