The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8+ T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848
Identifieur interne : 001535 ( Pmc/Curation ); précédent : 001534; suivant : 001536The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8+ T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848
Auteurs : Kai Zhao ; Hui Wang ; Changyou WuSource :
- Vaccine [ 0264-410X ] ; 2011.
Abstract
The induction of antigen specific memory CD8+ T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8+ T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8+ T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8+ T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8+ T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ+CD8+ T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8+ T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB+ and CD62L− and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.
Url:
DOI: 10.1016/j.vaccine.2011.06.100
PubMed: 21745520
PubMed Central: 7115397
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001535
Links to Exploration step
PMC:7115397Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup>
T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</title>
<author><name sortKey="Zhao, Kai" sort="Zhao, Kai" uniqKey="Zhao K" first="Kai" last="Zhao">Kai Zhao</name>
</author>
<author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name>
</author>
<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21745520</idno>
<idno type="pmc">7115397</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115397</idno>
<idno type="RBID">PMC:7115397</idno>
<idno type="doi">10.1016/j.vaccine.2011.06.100</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">001535</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001535</idno>
<idno type="wicri:Area/Pmc/Curation">001535</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001535</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup>
T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</title>
<author><name sortKey="Zhao, Kai" sort="Zhao, Kai" uniqKey="Zhao K" first="Kai" last="Zhao">Kai Zhao</name>
</author>
<author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name>
</author>
<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
</author>
</analytic>
<series><title level="j">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<idno type="eISSN">1873-2518</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The induction of antigen specific memory CD8<sup>+</sup>
T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8<sup>+</sup>
T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8<sup>+</sup>
T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8<sup>+</sup>
T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8<sup>+</sup>
T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ<sup>+</sup>
CD8<sup>+</sup>
T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8<sup>+</sup>
T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB<sup>+</sup>
and CD62L<sup>−</sup>
and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name>
</author>
<author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name>
</author>
<author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. Brodt</name>
</author>
<author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name>
</author>
<author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. Lai</name>
</author>
<author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name>
</author>
<author><name sortKey="Yam, L Y" uniqKey="Yam L">L.Y. Yam</name>
</author>
<author><name sortKey="Lim, W" uniqKey="Lim W">W. Lim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Epidemiology Consortium Csm" uniqKey="Epidemiology Consortium Csm">Epidemiology Consortium, CSM</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Shi, Z" uniqKey="Shi Z">Z. Shi</name>
</author>
<author><name sortKey="Yu, M" uniqKey="Yu M">M. Yu</name>
</author>
<author><name sortKey="Ren, W" uniqKey="Ren W">W. Ren</name>
</author>
<author><name sortKey="Smith, C" uniqKey="Smith C">C. Smith</name>
</author>
<author><name sortKey="Epstein, J H" uniqKey="Epstein J">J.H. Epstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Orellana, C" uniqKey="Orellana C">C. Orellana</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cao, W C" uniqKey="Cao W">W.C. Cao</name>
</author>
<author><name sortKey="Liu, W" uniqKey="Liu W">W. Liu</name>
</author>
<author><name sortKey="Zhang, P H" uniqKey="Zhang P">P.H. Zhang</name>
</author>
<author><name sortKey="Zhang, F" uniqKey="Zhang F">F. Zhang</name>
</author>
<author><name sortKey="Richardus, J H" uniqKey="Richardus J">J.H. Richardus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, W" uniqKey="Liu W">W. Liu</name>
</author>
<author><name sortKey="Fontanet, A" uniqKey="Fontanet A">A. Fontanet</name>
</author>
<author><name sortKey="Zhang, P H" uniqKey="Zhang P">P.H. Zhang</name>
</author>
<author><name sortKey="Zhan, L" uniqKey="Zhan L">L. Zhan</name>
</author>
<author><name sortKey="Xin, Z T" uniqKey="Xin Z">Z.T. Xin</name>
</author>
<author><name sortKey="Baril, L" uniqKey="Baril L">L. Baril</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fan, Y Y" uniqKey="Fan Y">Y.Y. Fan</name>
</author>
<author><name sortKey="Huang, Z T" uniqKey="Huang Z">Z.T. Huang</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L. Li</name>
</author>
<author><name sortKey="Wu, M H" uniqKey="Wu M">M.H. Wu</name>
</author>
<author><name sortKey="Yu, T" uniqKey="Yu T">T. Yu</name>
</author>
<author><name sortKey="Koup, R A" uniqKey="Koup R">R.A. Koup</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Herd, K A" uniqKey="Herd K">K.A. Herd</name>
</author>
<author><name sortKey="Mahalingam, S" uniqKey="Mahalingam S">S. Mahalingam</name>
</author>
<author><name sortKey="Mackay, I M" uniqKey="Mackay I">I.M. Mackay</name>
</author>
<author><name sortKey="Nissen, M" uniqKey="Nissen M">M. Nissen</name>
</author>
<author><name sortKey="Sloots, T P" uniqKey="Sloots T">T.P. Sloots</name>
</author>
<author><name sortKey="Tindle, R W" uniqKey="Tindle R">R.W. Tindle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tanabe, S" uniqKey="Tanabe S">S. Tanabe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hanke, T" uniqKey="Hanke T">T. Hanke</name>
</author>
<author><name sortKey="Neumann, V C" uniqKey="Neumann V">V.C. Neumann</name>
</author>
<author><name sortKey="Blanchard, T J" uniqKey="Blanchard T">T.J. Blanchard</name>
</author>
<author><name sortKey="Sweeney, P" uniqKey="Sweeney P">P. Sweeney</name>
</author>
<author><name sortKey="Hill, A V" uniqKey="Hill A">A.V. Hill</name>
</author>
<author><name sortKey="Smith, G L" uniqKey="Smith G">G.L. Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olsen, A W" uniqKey="Olsen A">A.W. Olsen</name>
</author>
<author><name sortKey="Hansen, P R" uniqKey="Hansen P">P.R. Hansen</name>
</author>
<author><name sortKey="Holm, A" uniqKey="Holm A">A. Holm</name>
</author>
<author><name sortKey="Andersen, P" uniqKey="Andersen P">P. Andersen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhao, K" uniqKey="Zhao K">K. Zhao</name>
</author>
<author><name sortKey="Yang, B" uniqKey="Yang B">B. Yang</name>
</author>
<author><name sortKey="Xu, Y" uniqKey="Xu Y">Y. Xu</name>
</author>
<author><name sortKey="Wu, C" uniqKey="Wu C">C. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lv, Y" uniqKey="Lv Y">Y. Lv</name>
</author>
<author><name sortKey="Ruan, Z" uniqKey="Ruan Z">Z. Ruan</name>
</author>
<author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name>
</author>
<author><name sortKey="Ni, B" uniqKey="Ni B">B. Ni</name>
</author>
<author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, J" uniqKey="Huang J">J. Huang</name>
</author>
<author><name sortKey="Cao, Y" uniqKey="Cao Y">Y. Cao</name>
</author>
<author><name sortKey="Du, J" uniqKey="Du J">J. Du</name>
</author>
<author><name sortKey="Bu, X" uniqKey="Bu X">X. Bu</name>
</author>
<author><name sortKey="Ma, R" uniqKey="Ma R">R. Ma</name>
</author>
<author><name sortKey="Wu, C" uniqKey="Wu C">C. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tsao, Y P" uniqKey="Tsao Y">Y.P. Tsao</name>
</author>
<author><name sortKey="Lin, J Y" uniqKey="Lin J">J.Y. Lin</name>
</author>
<author><name sortKey="Jan, J T" uniqKey="Jan J">J.T. Jan</name>
</author>
<author><name sortKey="Leng, C H" uniqKey="Leng C">C.H. Leng</name>
</author>
<author><name sortKey="Chu, C C" uniqKey="Chu C">C.C. Chu</name>
</author>
<author><name sortKey="Yang, Y C" uniqKey="Yang Y">Y.C. Yang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhou, M" uniqKey="Zhou M">M. Zhou</name>
</author>
<author><name sortKey="Xu, D" uniqKey="Xu D">D. Xu</name>
</author>
<author><name sortKey="Li, X" uniqKey="Li X">X. Li</name>
</author>
<author><name sortKey="Li, H" uniqKey="Li H">H. Li</name>
</author>
<author><name sortKey="Shan, M" uniqKey="Shan M">M. Shan</name>
</author>
<author><name sortKey="Tang, J" uniqKey="Tang J">J. Tang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, B" uniqKey="Wang B">B. Wang</name>
</author>
<author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name>
</author>
<author><name sortKey="Jiang, X" uniqKey="Jiang X">X. Jiang</name>
</author>
<author><name sortKey="Zhang, M" uniqKey="Zhang M">M. Zhang</name>
</author>
<author><name sortKey="Wan, T" uniqKey="Wan T">T. Wan</name>
</author>
<author><name sortKey="Li, N" uniqKey="Li N">N. Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ma, R" uniqKey="Ma R">R. Ma</name>
</author>
<author><name sortKey="Du, J L" uniqKey="Du J">J.L. Du</name>
</author>
<author><name sortKey="Huang, J" uniqKey="Huang J">J. Huang</name>
</author>
<author><name sortKey="Wu, C Y" uniqKey="Wu C">C.Y. Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shen, E X" uniqKey="Shen E">E.X. Shen</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L. Li</name>
</author>
<author><name sortKey="Li, L T" uniqKey="Li L">L.T. Li</name>
</author>
<author><name sortKey="Feng, L Q" uniqKey="Feng L">L.Q. Feng</name>
</author>
<author><name sortKey="Lu, L" uniqKey="Lu L">L. Lu</name>
</author>
<author><name sortKey="Yao, Z L" uniqKey="Yao Z">Z.L. Yao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Valensi, J P" uniqKey="Valensi J">J.P. Valensi</name>
</author>
<author><name sortKey="Carlson, J R" uniqKey="Carlson J">J.R. Carlson</name>
</author>
<author><name sortKey="Van Nest, G A" uniqKey="Van Nest G">G.A. Van Nest</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, Y" uniqKey="He Y">Y. He</name>
</author>
<author><name sortKey="Barker, S J" uniqKey="Barker S">S.J. Barker</name>
</author>
<author><name sortKey="Macdonald, A J" uniqKey="Macdonald A">A.J. MacDonald</name>
</author>
<author><name sortKey="Yu, Y" uniqKey="Yu Y">Y. Yu</name>
</author>
<author><name sortKey="Cao, L" uniqKey="Cao L">L. Cao</name>
</author>
<author><name sortKey="Li, J" uniqKey="Li J">J. Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matsumoto, M" uniqKey="Matsumoto M">M. Matsumoto</name>
</author>
<author><name sortKey="Seya, T" uniqKey="Seya T">T. Seya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Majewska, M" uniqKey="Majewska M">M. Majewska</name>
</author>
<author><name sortKey="Szczepanik, M" uniqKey="Szczepanik M">M. Szczepanik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Akira, S" uniqKey="Akira S">S. Akira</name>
</author>
<author><name sortKey="Takeda, K" uniqKey="Takeda K">K. Takeda</name>
</author>
<author><name sortKey="Kaisho, T" uniqKey="Kaisho T">T. Kaisho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gai, W W" uniqKey="Gai W">W.W. Gai</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name>
</author>
<author><name sortKey="Zhou, D H" uniqKey="Zhou D">D.H. Zhou</name>
</author>
<author><name sortKey="Chen, Y Q" uniqKey="Chen Y">Y.Q. Chen</name>
</author>
<author><name sortKey="Yang, J Y" uniqKey="Yang J">J.Y. Yang</name>
</author>
<author><name sortKey="Yan, H M" uniqKey="Yan H">H.M. Yan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krug, A" uniqKey="Krug A">A. Krug</name>
</author>
<author><name sortKey="Towarowski, A" uniqKey="Towarowski A">A. Towarowski</name>
</author>
<author><name sortKey="Britsch, S" uniqKey="Britsch S">S. Britsch</name>
</author>
<author><name sortKey="Rothenfusser, S" uniqKey="Rothenfusser S">S. Rothenfusser</name>
</author>
<author><name sortKey="Hornung, V" uniqKey="Hornung V">V. Hornung</name>
</author>
<author><name sortKey="Bals, R" uniqKey="Bals R">R. Bals</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mccluskie, M J" uniqKey="Mccluskie M">M.J. McCluskie</name>
</author>
<author><name sortKey="Cartier, J L" uniqKey="Cartier J">J.L. Cartier</name>
</author>
<author><name sortKey="Patrick, A J" uniqKey="Patrick A">A.J. Patrick</name>
</author>
<author><name sortKey="Sajic, D" uniqKey="Sajic D">D. Sajic</name>
</author>
<author><name sortKey="Weeratna, R D" uniqKey="Weeratna R">R.D. Weeratna</name>
</author>
<author><name sortKey="Rosenthal, K L" uniqKey="Rosenthal K">K.L. Rosenthal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rothenfusser, S" uniqKey="Rothenfusser S">S. Rothenfusser</name>
</author>
<author><name sortKey="Hornung, V" uniqKey="Hornung V">V. Hornung</name>
</author>
<author><name sortKey="Ayyoub, M" uniqKey="Ayyoub M">M. Ayyoub</name>
</author>
<author><name sortKey="Britsch, S" uniqKey="Britsch S">S. Britsch</name>
</author>
<author><name sortKey="Towarowski, A" uniqKey="Towarowski A">A. Towarowski</name>
</author>
<author><name sortKey="Krug, A" uniqKey="Krug A">A. Krug</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reis, E S C" uniqKey="Reis E">E.S.C. Reis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Edwards, A D" uniqKey="Edwards A">A.D. Edwards</name>
</author>
<author><name sortKey="Diebold, S S" uniqKey="Diebold S">S.S. Diebold</name>
</author>
<author><name sortKey="Slack, E M" uniqKey="Slack E">E.M. Slack</name>
</author>
<author><name sortKey="Tomizawa, H" uniqKey="Tomizawa H">H. Tomizawa</name>
</author>
<author><name sortKey="Hemmi, H" uniqKey="Hemmi H">H. Hemmi</name>
</author>
<author><name sortKey="Kaisho, T" uniqKey="Kaisho T">T. Kaisho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shortman, K" uniqKey="Shortman K">K. Shortman</name>
</author>
<author><name sortKey="Liu, Y J" uniqKey="Liu Y">Y.J. Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schlosser, E" uniqKey="Schlosser E">E. Schlosser</name>
</author>
<author><name sortKey="Mueller, M" uniqKey="Mueller M">M. Mueller</name>
</author>
<author><name sortKey="Fischer, S" uniqKey="Fischer S">S. Fischer</name>
</author>
<author><name sortKey="Basta, S" uniqKey="Basta S">S. Basta</name>
</author>
<author><name sortKey="Busch, D H" uniqKey="Busch D">D.H. Busch</name>
</author>
<author><name sortKey="Gander, B" uniqKey="Gander B">B. Gander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Salerno Goncalves, R" uniqKey="Salerno Goncalves R">R. Salerno-Goncalves</name>
</author>
<author><name sortKey="Sztein, M B" uniqKey="Sztein M">M.B. Sztein</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id>
<journal-id journal-id-type="iso-abbrev">Vaccine</journal-id>
<journal-title-group><journal-title>Vaccine</journal-title>
</journal-title-group>
<issn pub-type="ppub">0264-410X</issn>
<issn pub-type="epub">1873-2518</issn>
<publisher><publisher-name>Elsevier Ltd.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21745520</article-id>
<article-id pub-id-type="pmc">7115397</article-id>
<article-id pub-id-type="publisher-id">S0264-410X(11)00994-7</article-id>
<article-id pub-id-type="doi">10.1016/j.vaccine.2011.06.100</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8<sup>+</sup>
T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Zhao</surname>
<given-names>Kai</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Hui</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Wu</surname>
<given-names>Changyou</given-names>
</name>
<email>changyou_wu@yahoo.com</email>
<xref rid="cor0005" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff>Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, Guangdong Province 510080, People’ s Republic of China</aff>
<author-notes><corresp id="cor0005"><label>⁎</label>
Corresponding author. Tel.: +86 20 87332448; fax: +86 20 87332448. <email>changyou_wu@yahoo.com</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>13</day>
<month>7</month>
<year>2011</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>2</day>
<month>9</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>13</day>
<month>7</month>
<year>2011</year>
</pub-date>
<volume>29</volume>
<issue>38</issue>
<fpage>6670</fpage>
<lpage>6678</lpage>
<history><date date-type="received"><day>6</day>
<month>1</month>
<year>2011</year>
</date>
<date date-type="rev-recd"><day>13</day>
<month>6</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>25</day>
<month>6</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2011 Elsevier Ltd. All rights reserved.</copyright-statement>
<copyright-year>2011</copyright-year>
<copyright-holder>Elsevier Ltd</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract><p>The induction of antigen specific memory CD8<sup>+</sup>
T cells in vivo is very important to new vaccines against infectious diseases. In the present study, we aimed to evaluate the immune responses of peptide-specific CD8<sup>+</sup>
T cells induced by HLA-A*0201 restricted severe acute respiratory syndrome-associated coronavirus (SARS-CoV) S epitopes plus CpG oligodeoxynucleotide (CpG ODN), PolyI:C and R848 as adjuvants. Furthermore, the generation, distribution and phenotype of long-lasting peptide-specific memory CD8<sup>+</sup>
T cells were assessed by ELISA, ELISPOT and flow cytometry. Our results showed that antigen specific CD8<sup>+</sup>
T cells were elicited by HLA-A*0201 restricted SARS-CoV S epitopes. Furthermore, the frequency of peptide-specific CD8<sup>+</sup>
T cells was dramatically increased after both prime and boost immunization with peptides plus CpG ODN, whereas slight enhancements were induced following boost vaccination with peptides plus PolyI:C or R848. SARS-CoV S peptide-specific IFN-γ<sup>+</sup>
CD8<sup>+</sup>
T cells were distributed throughout the lymphoid and non-lymphoid tissues. Results also demonstrated that the HLA-A*0201 restricted peptide-specific CD8<sup>+</sup>
T cells induced by peptides plus CpG ODN carried a memory cell phenotype with CD45RB<sup>+</sup>
and CD62L<sup>−</sup>
and possessed long-term survival ability in vivo. Taken together, our results implied that HLA-A*0201 restricted SARS-CoV S epitopes plus CpG ODN might be the superior candidates for SARS vaccine.</p>
</abstract>
<kwd-group><title>Abbreviations</title>
<kwd>SARS, severe acute respiratory syndrome</kwd>
<kwd>SARS-CoV, SARS-associated coronavirus</kwd>
<kwd>CpG ODN, CpG oligodeoxynucleotides</kwd>
<kwd>TLR, toll-like receptor</kwd>
</kwd-group>
<kwd-group><title>Keywords</title>
<kwd>SARS-CoV</kwd>
<kwd>Epitope</kwd>
<kwd>Adjuvant</kwd>
<kwd>T cell</kwd>
<kwd>Vaccine</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001535 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 001535 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:7115397 |texte= The immune responses of HLA-A*0201 restricted SARS-CoV S peptide-specific CD8+ T cells are augmented in varying degrees by CpG ODN, PolyI:C and R848 }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:21745520" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |