Reconstitution of the receptor-binding motif of the SARS coronavirus
Identifieur interne : 001442 ( Pmc/Curation ); précédent : 001441; suivant : 001443Reconstitution of the receptor-binding motif of the SARS coronavirus
Auteurs : Natalia T. Freund [Israël, États-Unis] ; Anna Roitburd-Berman [Israël] ; Jianhua Sui [États-Unis, République populaire de Chine] ; Wayne A. Marasco [États-Unis] ; Jonathan M. Gershoni [Israël]Source :
- Protein Engineering, Design and Selection [ 1741-0126 ] ; 2015.
Abstract
The severe acute respiratory syndrome (SARS) coronavirus (CoV) identified in 2003 has infected ∼8000 people worldwide, killing nearly 10% of them. The infection of target cells by the SARS CoV is mediated through the interaction of the viral Spike (S) protein (1255 amino acids) and its cellular receptor, angiotensin-converting enzyme 2 (ACE2). The SARS CoV receptor-binding domain (amino acids N318–T509 of S protein) harbors an extended excursion along its periphery that contacts ACE2 and is designated the receptor-binding motif (RBM, amino acids S432–T486). In addition, the RBM is a major antigenic determinant, able to elicit production of neutralizing antibodies. Hence, the role of the RBM is a bi-functional bioactive surface that can be demonstrated by antibodies such as the neutralizing human anti-SARS monoclonal antibody (mAb) 80R which targets the RBM and competes with the ACE2 receptor for binding. Here, we employ phage-display peptide-libraries to reconstitute a functional RBM. This is achieved by generating a vast collection of candidate RBM peptides that present a diversity of conformations. Screening such ‘Conformer Libraries’ with corresponding ligands has produced short RBM constructs (ca. 40 amino acids) that can bind both the ACE2 receptor and the neutralizing mAb 80R.
Url:
DOI: 10.1093/protein/gzv052
PubMed: 26487711
PubMed Central: 7107155
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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>The severe acute respiratory syndrome (SARS) coronavirus (CoV) identified in 2003 has infected ∼8000 people worldwide, killing nearly 10% of them. The infection of target cells by the SARS CoV is mediated through the interaction of the viral Spike (S) protein (1255 amino acids) and its cellular receptor, angiotensin-converting enzyme 2 (ACE2). The SARS CoV receptor-binding domain (amino acids N318–T509 of S protein) harbors an extended excursion along its periphery that contacts ACE2 and is designated the receptor-binding motif (RBM, amino acids S432–T486). In addition, the RBM is a major antigenic determinant, able to elicit production of neutralizing antibodies. Hence, the role of the RBM is a bi-functional bioactive surface that can be demonstrated by antibodies such as the neutralizing human anti-SARS monoclonal antibody (mAb) 80R which targets the RBM and competes with the ACE2 receptor for binding. Here, we employ phage-display peptide-libraries to reconstitute a functional RBM. This is achieved by generating a vast collection of candidate RBM peptides that present a diversity of conformations. Screening such ‘Conformer Libraries’ with corresponding ligands has produced short RBM constructs (ca. 40 amino acids) that can bind both the ACE2 receptor and the neutralizing mAb 80R.</p>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Babcock, G J" uniqKey="Babcock G">G.J. Babcock</name>
</author>
<author><name sortKey="Esshaki, D J" uniqKey="Esshaki D">D.J. Esshaki</name>
</author>
<author><name sortKey="Thomas, W D" uniqKey="Thomas W">W.D. Thomas</name>
</author>
<author><name sortKey="Ambrosino, D M" uniqKey="Ambrosino D">D.M. Ambrosino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bailey, T L" uniqKey="Bailey T">T.L. Bailey</name>
</author>
<author><name sortKey="Boden, M" uniqKey="Boden M">M. Boden</name>
</author>
<author><name sortKey="Buske, F A" uniqKey="Buske F">F.A. Buske</name>
</author>
<author><name sortKey="Frith, M" uniqKey="Frith M">M. Frith</name>
</author>
<author><name sortKey="Grant, C E" uniqKey="Grant C">C.E. Grant</name>
</author>
<author><name sortKey="Clementi, L" uniqKey="Clementi L">L. Clementi</name>
</author>
<author><name sortKey="Ren, J" uniqKey="Ren J">J. Ren</name>
</author>
<author><name sortKey="Li, W W" uniqKey="Li W">W.W. Li</name>
</author>
<author><name sortKey="Noble, W S" uniqKey="Noble W">W.S. Noble</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bonavia, A" uniqKey="Bonavia A">A. Bonavia</name>
</author>
<author><name sortKey="Zelus, B D" uniqKey="Zelus B">B.D. Zelus</name>
</author>
<author><name sortKey="Wentworth, D E" uniqKey="Wentworth D">D.E. Wentworth</name>
</author>
<author><name sortKey="Talbot, P J" uniqKey="Talbot P">P.J. Talbot</name>
</author>
<author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Breslin, J J" uniqKey="Breslin J">J.J. Breslin</name>
</author>
<author><name sortKey="Mork, I" uniqKey="Mork I">I. Mork</name>
</author>
<author><name sortKey="Smith, M K" uniqKey="Smith M">M.K. Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bublil, E M" uniqKey="Bublil E">E.M. Bublil</name>
</author>
<author><name sortKey="Yeger Azuz, S" uniqKey="Yeger Azuz S">S. Yeger-Azuz</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bublil, E M" uniqKey="Bublil E">E.M. Bublil</name>
</author>
<author><name sortKey="Freund, N T" uniqKey="Freund N">N.T. Freund</name>
</author>
<author><name sortKey="Mayrose, I" uniqKey="Mayrose I">I. Mayrose</name>
</author>
<author><name sortKey="Penn, O" uniqKey="Penn O">O. Penn</name>
</author>
<author><name sortKey="Roitburd Berman, A" uniqKey="Roitburd Berman A">A. Roitburd-Berman</name>
</author>
<author><name sortKey="Rubinstein, N D" uniqKey="Rubinstein N">N.D. Rubinstein</name>
</author>
<author><name sortKey="Pupko, T" uniqKey="Pupko T">T. Pupko</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chang, H H" uniqKey="Chang H">H.H. Chang</name>
</author>
<author><name sortKey="Chen, P K" uniqKey="Chen P">P.K. Chen</name>
</author>
<author><name sortKey="Lin, G L" uniqKey="Lin G">G.L. Lin</name>
</author>
<author><name sortKey="Wang, C J" uniqKey="Wang C">C.J. Wang</name>
</author>
<author><name sortKey="Liao, C H" uniqKey="Liao C">C.H. Liao</name>
</author>
<author><name sortKey="Hsiao, Y C" uniqKey="Hsiao Y">Y.C. Hsiao</name>
</author>
<author><name sortKey="Dong, J H" uniqKey="Dong J">J.H. Dong</name>
</author>
<author><name sortKey="Sun, D S" uniqKey="Sun D">D.S. Sun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Coughlin, M M" uniqKey="Coughlin M">M.M. Coughlin</name>
</author>
<author><name sortKey="Prabhakar, B S" uniqKey="Prabhakar B">B.S. Prabhakar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name>
</author>
<author><name sortKey="He, Y" uniqKey="He Y">Y. He</name>
</author>
<author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name>
</author>
<author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name>
</author>
<author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J. Zheng</name>
</author>
<author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dveksler, G S" uniqKey="Dveksler G">G.S. Dveksler</name>
</author>
<author><name sortKey="Pensiero, M N" uniqKey="Pensiero M">M.N. Pensiero</name>
</author>
<author><name sortKey="Cardellichio, C B" uniqKey="Cardellichio C">C.B. Cardellichio</name>
</author>
<author><name sortKey="Williams, R K" uniqKey="Williams R">R.K. Williams</name>
</author>
<author><name sortKey="Jiang, G S" uniqKey="Jiang G">G.S. Jiang</name>
</author>
<author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name>
</author>
<author><name sortKey="Dieffenbach, C W" uniqKey="Dieffenbach C">C.W. Dieffenbach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dveksler, G S" uniqKey="Dveksler G">G.S. Dveksler</name>
</author>
<author><name sortKey="Dieffenbach, C W" uniqKey="Dieffenbach C">C.W. Dieffenbach</name>
</author>
<author><name sortKey="Cardellichio, C B" uniqKey="Cardellichio C">C.B. Cardellichio</name>
</author>
<author><name sortKey="Mccuaig, K" uniqKey="Mccuaig K">K. McCuaig</name>
</author>
<author><name sortKey="Pensiero, M N" uniqKey="Pensiero M">M.N. Pensiero</name>
</author>
<author><name sortKey="Jiang, G S" uniqKey="Jiang G">G.S. Jiang</name>
</author>
<author><name sortKey="Beauchemin, N" uniqKey="Beauchemin N">N. Beauchemin</name>
</author>
<author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Enshell Seijffers, D" uniqKey="Enshell Seijffers D">D. Enshell-Seijffers</name>
</author>
<author><name sortKey="Smelyanski, L" uniqKey="Smelyanski L">L. Smelyanski</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fellouse, F A" uniqKey="Fellouse F">F.A. Fellouse</name>
</author>
<author><name sortKey="Wiesmann, C" uniqKey="Wiesmann C">C. Wiesmann</name>
</author>
<author><name sortKey="Sidhu, S S" uniqKey="Sidhu S">S.S. Sidhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fellouse, F A" uniqKey="Fellouse F">F.A. Fellouse</name>
</author>
<author><name sortKey="Li, B" uniqKey="Li B">B. Li</name>
</author>
<author><name sortKey="Compaan, D M" uniqKey="Compaan D">D.M. Compaan</name>
</author>
<author><name sortKey="Peden, A A" uniqKey="Peden A">A.A. Peden</name>
</author>
<author><name sortKey="Hymowitz, S G" uniqKey="Hymowitz S">S.G. Hymowitz</name>
</author>
<author><name sortKey="Sidhu, S S" uniqKey="Sidhu S">S.S. Sidhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Freund, N T" uniqKey="Freund N">N.T. Freund</name>
</author>
<author><name sortKey="Enshell Seijffers, D" uniqKey="Enshell Seijffers D">D. Enshell-Seijffers</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
<author><name sortKey="Roitburd Berman, A" uniqKey="Roitburd Berman A">A. Roitburd-Berman</name>
</author>
<author><name sortKey="Siman Tov, D D" uniqKey="Siman Tov D">D.D. Siman-Tov</name>
</author>
<author><name sortKey="Tarnovitski Freund, N" uniqKey="Tarnovitski Freund N">N. Tarnovitski Freund</name>
</author>
<author><name sortKey="Weiss, Y" uniqKey="Weiss Y">Y. Weiss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ho, T Y" uniqKey="Ho T">T.Y. Ho</name>
</author>
<author><name sortKey="Wu, S L" uniqKey="Wu S">S.L. Wu</name>
</author>
<author><name sortKey="Chen, J C" uniqKey="Chen J">J.C. Chen</name>
</author>
<author><name sortKey="Wei, Y C" uniqKey="Wei Y">Y.C. Wei</name>
</author>
<author><name sortKey="Cheng, S E" uniqKey="Cheng S">S.E. Cheng</name>
</author>
<author><name sortKey="Chang, Y H" uniqKey="Chang Y">Y.H. Chang</name>
</author>
<author><name sortKey="Liu, H J" uniqKey="Liu H">H.J. Liu</name>
</author>
<author><name sortKey="Hsiang, C Y" uniqKey="Hsiang C">C.Y. Hsiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hu, H" uniqKey="Hu H">H. Hu</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L. Li</name>
</author>
<author><name sortKey="Kao, R Y" uniqKey="Kao R">R.Y. Kao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hwang, W C" uniqKey="Hwang W">W.C. Hwang</name>
</author>
<author><name sortKey="Lin, Y" uniqKey="Lin Y">Y. Lin</name>
</author>
<author><name sortKey="Santelli, E" uniqKey="Santelli E">E. Santelli</name>
</author>
<author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
<author><name sortKey="Jaroszewski, L" uniqKey="Jaroszewski L">L. Jaroszewski</name>
</author>
<author><name sortKey="Stec, B" uniqKey="Stec B">B. Stec</name>
</author>
<author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name>
</author>
<author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name>
</author>
<author><name sortKey="Liddington, R C" uniqKey="Liddington R">R.C. Liddington</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaplan, G" uniqKey="Kaplan G">G. Kaplan</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kubo, H" uniqKey="Kubo H">H. Kubo</name>
</author>
<author><name sortKey="Yamada, Y K" uniqKey="Yamada Y">Y.K. Yamada</name>
</author>
<author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name>
</author>
<author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N. Vasilieva</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name>
</author>
<author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name>
</author>
<author><name sortKey="Jones, S J" uniqKey="Jones S">S.J. Jones</name>
</author>
<author><name sortKey="Astell, C R" uniqKey="Astell C">C.R. Astell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mayrose, I" uniqKey="Mayrose I">I. Mayrose</name>
</author>
<author><name sortKey="Shlomi, T" uniqKey="Shlomi T">T. Shlomi</name>
</author>
<author><name sortKey="Rubinstein, N D" uniqKey="Rubinstein N">N.D. Rubinstein</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
<author><name sortKey="Ruppin, E" uniqKey="Ruppin E">E. Ruppin</name>
</author>
<author><name sortKey="Sharan, R" uniqKey="Sharan R">R. Sharan</name>
</author>
<author><name sortKey="Pupko, T" uniqKey="Pupko T">T. Pupko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mayrose, I" uniqKey="Mayrose I">I. Mayrose</name>
</author>
<author><name sortKey="Penn, O" uniqKey="Penn O">O. Penn</name>
</author>
<author><name sortKey="Erez, E" uniqKey="Erez E">E. Erez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name>
</author>
<author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Prabakaran, P" uniqKey="Prabakaran P">P. Prabakaran</name>
</author>
<author><name sortKey="Gan, J" uniqKey="Gan J">J. Gan</name>
</author>
<author><name sortKey="Feng, Y" uniqKey="Feng Y">Y. Feng</name>
</author>
<author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z. Zhu</name>
</author>
<author><name sortKey="Choudhry, V" uniqKey="Choudhry V">V. Choudhry</name>
</author>
<author><name sortKey="Xiao, X" uniqKey="Xiao X">X. Xiao</name>
</author>
<author><name sortKey="Ji, X" uniqKey="Ji X">X. Ji</name>
</author>
<author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rani, M" uniqKey="Rani M">M. Rani</name>
</author>
<author><name sortKey="Bolles, M" uniqKey="Bolles M">M. Bolles</name>
</author>
<author><name sortKey="Donaldson, E F" uniqKey="Donaldson E">E.F. Donaldson</name>
</author>
<author><name sortKey="Van Blarcom, T" uniqKey="Van Blarcom T">T. Van Blarcom</name>
</author>
<author><name sortKey="Baric, R" uniqKey="Baric R">R. Baric</name>
</author>
<author><name sortKey="Iverson, B" uniqKey="Iverson B">B. Iverson</name>
</author>
<author><name sortKey="Georgiou, G" uniqKey="Georgiou G">G. Georgiou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rockx, B" uniqKey="Rockx B">B. Rockx</name>
</author>
<author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name>
</author>
<author><name sortKey="Donaldson, E" uniqKey="Donaldson E">E. Donaldson</name>
</author>
<author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T. Sheahan</name>
</author>
<author><name sortKey="Stadler, K" uniqKey="Stadler K">K. Stadler</name>
</author>
<author><name sortKey="Lanzavecchia, A" uniqKey="Lanzavecchia A">A. Lanzavecchia</name>
</author>
<author><name sortKey="Baric, R" uniqKey="Baric R">R. Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rockx, B" uniqKey="Rockx B">B. Rockx</name>
</author>
<author><name sortKey="Donaldson, E" uniqKey="Donaldson E">E. Donaldson</name>
</author>
<author><name sortKey="Frieman, M" uniqKey="Frieman M">M. Frieman</name>
</author>
<author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T. Sheahan</name>
</author>
<author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name>
</author>
<author><name sortKey="Lanzavecchia, A" uniqKey="Lanzavecchia A">A. Lanzavecchia</name>
</author>
<author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name>
</author>
<author><name sortKey="Oberste, M S" uniqKey="Oberste M">M.S. Oberste</name>
</author>
<author><name sortKey="Monroe, S S" uniqKey="Monroe S">S.S. Monroe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rubinstein, N D" uniqKey="Rubinstein N">N.D. Rubinstein</name>
</author>
<author><name sortKey="Mayrose, I" uniqKey="Mayrose I">I. Mayrose</name>
</author>
<author><name sortKey="Halperin, D" uniqKey="Halperin D">D. Halperin</name>
</author>
<author><name sortKey="Yekutieli, D" uniqKey="Yekutieli D">D. Yekutieli</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
<author><name sortKey="Pupko, T" uniqKey="Pupko T">T. Pupko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Siman Tov, D D" uniqKey="Siman Tov D">D.D. Siman-Tov</name>
</author>
<author><name sortKey="Zemel, R" uniqKey="Zemel R">R. Zemel</name>
</author>
<author><name sortKey="Tur Kaspa, R" uniqKey="Tur Kaspa R">R. Tur Kaspa</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Struck, A W" uniqKey="Struck A">A.W. Struck</name>
</author>
<author><name sortKey="Axmann, M" uniqKey="Axmann M">M. Axmann</name>
</author>
<author><name sortKey="Pfefferle, S" uniqKey="Pfefferle S">S. Pfefferle</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Murakami, A" uniqKey="Murakami A">A. Murakami</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Roberts, A" uniqKey="Roberts A">A. Roberts</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
<author><name sortKey="Aird, D R" uniqKey="Aird D">D.R. Aird</name>
</author>
<author><name sortKey="Tamin, A" uniqKey="Tamin A">A. Tamin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tarnovitski, N" uniqKey="Tarnovitski N">N. Tarnovitski</name>
</author>
<author><name sortKey="Matthews, L J" uniqKey="Matthews L">L.J. Matthews</name>
</author>
<author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name>
</author>
<author><name sortKey="Gershoni, J M" uniqKey="Gershoni J">J.M. Gershoni</name>
</author>
<author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K. Wong</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name>
</author>
<author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name>
</author>
<author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xiao, X" uniqKey="Xiao X">X. Xiao</name>
</author>
<author><name sortKey="Chakraborti, S" uniqKey="Chakraborti S">S. Chakraborti</name>
</author>
<author><name sortKey="Dimitrov, A S" uniqKey="Dimitrov A">A.S. Dimitrov</name>
</author>
<author><name sortKey="Gramatikoff, K" uniqKey="Gramatikoff K">K. Gramatikoff</name>
</author>
<author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yeager, C L" uniqKey="Yeager C">C.L. Yeager</name>
</author>
<author><name sortKey="Ashmun, R A" uniqKey="Ashmun R">R.A. Ashmun</name>
</author>
<author><name sortKey="Williams, R K" uniqKey="Williams R">R.K. Williams</name>
</author>
<author><name sortKey="Cardellichio, C B" uniqKey="Cardellichio C">C.B. Cardellichio</name>
</author>
<author><name sortKey="Shapiro, L H" uniqKey="Shapiro L">L.H. Shapiro</name>
</author>
<author><name sortKey="Look, A T" uniqKey="Look A">A.T. Look</name>
</author>
<author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z. Zhu</name>
</author>
<author><name sortKey="Chakraborti, S" uniqKey="Chakraborti S">S. Chakraborti</name>
</author>
<author><name sortKey="He, Y" uniqKey="He Y">Y. He</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Protein Eng Des Sel</journal-id>
<journal-id journal-id-type="iso-abbrev">Protein Eng. Des. Sel</journal-id>
<journal-id journal-id-type="publisher-id">proeng</journal-id>
<journal-id journal-id-type="hwp">proeng</journal-id>
<journal-title-group><journal-title>Protein Engineering, Design and Selection</journal-title>
</journal-title-group>
<issn pub-type="ppub">1741-0126</issn>
<issn pub-type="epub">1741-0134</issn>
<publisher><publisher-name>Oxford University Press</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26487711</article-id>
<article-id pub-id-type="pmc">7107155</article-id>
<article-id pub-id-type="doi">10.1093/protein/gzv052</article-id>
<article-id pub-id-type="publisher-id">gzv052</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Reconstitution of the receptor-binding motif of the SARS coronavirus</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Freund</surname>
<given-names>Natalia T.</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="aff" rid="af4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Roitburd-Berman</surname>
<given-names>Anna</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sui</surname>
<given-names>Jianhua</given-names>
</name>
<xref ref-type="aff" rid="af2">2</xref>
<xref ref-type="aff" rid="af3">3</xref>
<xref ref-type="aff" rid="af5">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Marasco</surname>
<given-names>Wayne A.</given-names>
</name>
<xref ref-type="aff" rid="af2">2</xref>
<xref ref-type="aff" rid="af3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gershoni</surname>
<given-names>Jonathan M.</given-names>
</name>
<xref ref-type="aff" rid="af1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
<pmc-comment>gershoni@tauex.tau.ac.il </pmc-comment>
</contrib>
</contrib-group>
<aff id="af1"><label>1</label>
<addr-line>Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences</addr-line>
,<institution>Tel Aviv University</institution>
,<addr-line>Tel Aviv 69978</addr-line>
,<country>Israel</country>
</aff>
<aff id="af2"><label>2</label>
<addr-line>Department of Cancer Immunology and AIDS</addr-line>
,<institution>Dana Farber Cancer Institute</institution>
</aff>
<aff id="af3"><label>3</label>
<addr-line>Department of Medicine</addr-line>
,<institution>Harvard Medical School</institution>
,<addr-line>Boston, MA 02115</addr-line>
,<country>USA</country>
</aff>
<aff id="af4"><label>4</label>
<addr-line>Present address: Laboratory of Molecular Immunology</addr-line>
,<institution>The Rockefeller University</institution>
,<addr-line>New York, NY 10065</addr-line>
,<country>USA</country>
</aff>
<aff id="af5"><label>5</label>
<institution>Present address: National Institute of Biological Sciences</institution>
,<addr-line>Beijing 102206</addr-line>
,<country>China</country>
</aff>
<author-notes><corresp id="cor1"><label>*</label>
To whom correspondence should be addressed. E-mail: <email>gershoni@tauex.tau.ac.il</email>
</corresp>
<fn id="AN1" fn-type="con"><p>Edited by Dennis Burton</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>12</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub" iso-8601-date="2015-10-20"><day>20</day>
<month>10</month>
<year>2015</year>
</pub-date>
<volume>28</volume>
<issue>12</issue>
<fpage>567</fpage>
<lpage>575</lpage>
<history><date date-type="received"><day>27</day>
<month>7</month>
<year>2015</year>
</date>
<date date-type="rev-recd"><day>27</day>
<month>7</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>8</day>
<month>9</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com</copyright-statement>
<copyright-year>2015</copyright-year>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.</license-p>
</license>
</permissions>
<self-uri xlink:href="gzv052.pdf"></self-uri>
<abstract><title>Abstract</title>
<p>The severe acute respiratory syndrome (SARS) coronavirus (CoV) identified in 2003 has infected ∼8000 people worldwide, killing nearly 10% of them. The infection of target cells by the SARS CoV is mediated through the interaction of the viral Spike (S) protein (1255 amino acids) and its cellular receptor, angiotensin-converting enzyme 2 (ACE2). The SARS CoV receptor-binding domain (amino acids N318–T509 of S protein) harbors an extended excursion along its periphery that contacts ACE2 and is designated the receptor-binding motif (RBM, amino acids S432–T486). In addition, the RBM is a major antigenic determinant, able to elicit production of neutralizing antibodies. Hence, the role of the RBM is a bi-functional bioactive surface that can be demonstrated by antibodies such as the neutralizing human anti-SARS monoclonal antibody (mAb) 80R which targets the RBM and competes with the ACE2 receptor for binding. Here, we employ phage-display peptide-libraries to reconstitute a functional RBM. This is achieved by generating a vast collection of candidate RBM peptides that present a diversity of conformations. Screening such ‘Conformer Libraries’ with corresponding ligands has produced short RBM constructs (ca. 40 amino acids) that can bind both the ACE2 receptor and the neutralizing mAb 80R.</p>
</abstract>
<kwd-group><kwd>bioactive peptide</kwd>
<kwd>conformational library</kwd>
<kwd>phage-display</kwd>
<kwd>virus spike</kwd>
</kwd-group>
<funding-group><award-group><funding-source><named-content content-type="funder-name">US-Israel Binational Science</named-content>
</funding-source>
</award-group>
<award-group><funding-source><named-content content-type="funder-name">Foundation</named-content>
</funding-source>
<award-id>2005290 to J.M.G. and W.A.M</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
</record>
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