Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV
Identifieur interne : 001227 ( Pmc/Curation ); précédent : 001226; suivant : 001228Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV
Auteurs : Miriam E. R. Darnell [États-Unis] ; Kanta Subbarao [États-Unis] ; Stephen M. Feinstone [États-Unis] ; Deborah R. Taylor [États-Unis]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2004.
Abstract
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.
Url:
DOI: 10.1016/j.jviromet.2004.06.006
PubMed: 15350737
PubMed Central: 7112912
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Taylor</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15350737</idno><idno type="pmc">7112912</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112912</idno><idno type="RBID">PMC:7112912</idno><idno type="doi">10.1016/j.jviromet.2004.06.006</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">001227</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001227</idno><idno type="wicri:Area/Pmc/Curation">001227</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001227</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV</title><author><name sortKey="Darnell, Miriam E R" sort="Darnell, Miriam E R" uniqKey="Darnell M" first="Miriam E. R." last="Darnell">Miriam E. R. Darnell</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892</wicri:regionArea></affiliation></author><author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><affiliation wicri:level="1"><nlm:aff id="aff2">Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892</wicri:regionArea></affiliation></author><author><name sortKey="Feinstone, Stephen M" sort="Feinstone, Stephen M" uniqKey="Feinstone S" first="Stephen M." last="Feinstone">Stephen M. Feinstone</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892</wicri:regionArea></affiliation></author><author><name sortKey="Taylor, Deborah R" sort="Taylor, Deborah R" uniqKey="Taylor D" first="Deborah R." last="Taylor">Deborah R. Taylor</name><affiliation wicri:level="1"><nlm:aff id="aff1">Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol Methods</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol. Methods</journal-id><journal-title-group><journal-title>Journal of Virological Methods</journal-title></journal-title-group><issn pub-type="ppub">0166-0934</issn><issn pub-type="epub">1879-0984</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15350737</article-id><article-id pub-id-type="pmc">7112912</article-id><article-id pub-id-type="publisher-id">S0166-0934(04)00179-X</article-id><article-id pub-id-type="doi">10.1016/j.jviromet.2004.06.006</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Darnell</surname><given-names>Miriam E.R.</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Subbarao</surname><given-names>Kanta</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Feinstone</surname><given-names>Stephen M.</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Taylor</surname><given-names>Deborah R.</given-names></name><email>taylord@cber.fda.gov</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">*</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA</aff><aff id="aff2"><label>b</label>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Tel.: +1 301 827 1878; fax: +1 301 496 1810. <email>taylord@cber.fda.gov</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>3</day><month>8</month><year>2004</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>10</month><year>2004</year></pub-date><pub-date pub-type="epub"><day>3</day><month>8</month><year>2004</year></pub-date><volume>121</volume><issue>1</issue><fpage>85</fpage><lpage>91</lpage><history><date date-type="received"><day>27</day><month>5</month><year>2004</year></date><date date-type="accepted"><day>15</day><month>6</month><year>2004</year></date></history><permissions><copyright-statement>Copyright © 2004 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2004</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>Coronavirus</kwd><kwd>Virus inactivation</kwd><kwd>Tissue culture</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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