Mosaic Structure of Human Coronavirus NL63, One Thousand Years of Evolution
Identifieur interne : 001199 ( Pmc/Curation ); précédent : 001198; suivant : 001200Mosaic Structure of Human Coronavirus NL63, One Thousand Years of Evolution
Auteurs : Krzysztof Pyrc [Pays-Bas] ; Ronald Dijkman [Pays-Bas] ; Lea Deng [Nouvelle-Zélande] ; Maarten F. Jebbink [Pays-Bas] ; Howard A. Ross [Nouvelle-Zélande] ; Ben Berkhout [Pays-Bas] ; Lia Van Der Hoek [Pays-Bas]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2006.
Abstract
Before the SARS outbreak only two human coronaviruses (HCoV) were known: HCoV-OC43 and HCoV-229E. With the discovery of SARS-CoV in 2003, a third family member was identified. Soon thereafter, we described the fourth human coronavirus (HCoV-NL63), a virus that has spread worldwide and is associated with croup in children. We report here the complete genome sequence of two HCoV-NL63 clinical isolates, designated Amsterdam 57 and Amsterdam 496. The genomes are 27,538 and 27,550 nucleotides long, respectively, and share the same genome organization. We identified two variable regions, one within the 1a and one within the S gene, whereas the 1b and N genes were most conserved. Phylogenetic analysis revealed that HCoV-NL63 genomes have a mosaic structure with multiple recombination sites. Additionally, employing three different algorithms, we assessed the evolutionary rate for the S gene of group Ib coronaviruses to be ∼ 3 × 10− 4 substitutions per site per year. Using this evolutionary rate we determined that HCoV-NL63 diverged in the 11th century from its closest relative HCoV-229E.
Url:
DOI: 10.1016/j.jmb.2006.09.074
PubMed: 17054987
PubMed Central: 7094706
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Jebbink</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam</wicri:regionArea></affiliation></author><author><name sortKey="Ross, Howard A" sort="Ross, Howard A" uniqKey="Ross H" first="Howard A." last="Ross">Howard A. Ross</name><affiliation wicri:level="1"><nlm:aff id="aff2">School of Biological Sciences and Bioinformatics Institute, University of Auckland, Private Bag 92019, Auckland, New Zealand</nlm:aff><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Biological Sciences and Bioinformatics Institute, University of Auckland, Private Bag 92019, Auckland</wicri:regionArea></affiliation></author><author><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam</wicri:regionArea></affiliation></author><author><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Molecular Biology</title><idno type="ISSN">0022-2836</idno><idno type="eISSN">1089-8638</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Before the SARS outbreak only two human coronaviruses (HCoV) were known: HCoV-OC43 and HCoV-229E. With the discovery of SARS-CoV in 2003, a third family member was identified. Soon thereafter, we described the fourth human coronavirus (HCoV-NL63), a virus that has spread worldwide and is associated with croup in children. We report here the complete genome sequence of two HCoV-NL63 clinical isolates, designated Amsterdam 57 and Amsterdam 496. The genomes are 27,538 and 27,550 nucleotides long, respectively, and share the same genome organization. We identified two variable regions, one within the 1a and one within the S gene, whereas the 1b and N genes were most conserved. Phylogenetic analysis revealed that HCoV-NL63 genomes have a mosaic structure with multiple recombination sites. Additionally, employing three different algorithms, we assessed the evolutionary rate for the S gene of group Ib coronaviruses to be ∼ 3 × 10<sup>− 4</sup> substitutions per site per year. Using this evolutionary rate we determined that HCoV-NL63 diverged in the 11th century from its closest relative HCoV-229E.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Guy, J S" uniqKey="Guy J">J.S. Guy</name></author><author><name sortKey="Breslin, J J" uniqKey="Breslin J">J.J. Breslin</name></author><author><name sortKey="Breuhaus, B" uniqKey="Breuhaus B">B. Breuhaus</name></author><author><name sortKey="Vivrette, S" uniqKey="Vivrette S">S. Vivrette</name></author><author><name sortKey="Smith, L G" uniqKey="Smith L">L.G. Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, M M C" uniqKey="Lai M">M.M.C. Lai</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name></author><author><name sortKey="Brian, D A" uniqKey="Brian D">D.A. Brian</name></author><author><name sortKey="Brinton, M A" uniqKey="Brinton M">M.A. Brinton</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author><author><name sortKey="Horzinek, M C" uniqKey="Horzinek M">M.C. Horzinek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author><author><name sortKey="Vermeulen Oost, W" uniqKey="Vermeulen Oost W">W. Vermeulen-Oost</name></author><author><name sortKey="Berkhout, R J" uniqKey="Berkhout R">R.J. Berkhout</name></author><author><name sortKey="Wolthers, K C" uniqKey="Wolthers K">K.C. Wolthers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B. Berkhout</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B. Berkhout</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author><author><name sortKey="Rottier, P" uniqKey="Rottier P">P. Rottier</name></author><author><name sortKey="Van Der, H L" uniqKey="Van Der H">H.L. van der</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Sure, K" uniqKey="Sure K">K. Sure</name></author><author><name sortKey="Ihorst, G" uniqKey="Ihorst G">G. Ihorst</name></author><author><name sortKey="Stang, A" uniqKey="Stang A">A. Stang</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ebihara, T" uniqKey="Ebihara T">T. Ebihara</name></author><author><name sortKey="Endo, R" uniqKey="Endo R">R. Endo</name></author><author><name sortKey="Ma, X" uniqKey="Ma X">X. Ma</name></author><author><name sortKey="Ishiguro, N" uniqKey="Ishiguro N">N. Ishiguro</name></author><author><name sortKey="Kikuta, H" uniqKey="Kikuta H">H. Kikuta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chiu, S S" uniqKey="Chiu S">S.S. Chiu</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Chu, K W" uniqKey="Chu K">K.W. Chu</name></author><author><name sortKey="Kwan, S W" uniqKey="Kwan S">S.W. Kwan</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arden, K E" uniqKey="Arden K">K.E. Arden</name></author><author><name sortKey="Nissen, M D" uniqKey="Nissen M">M.D. Nissen</name></author><author><name sortKey="Sloots, T P" uniqKey="Sloots T">T.P. Sloots</name></author><author><name sortKey="Mackay, I M" uniqKey="Mackay I">I.M. Mackay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Esper, F" uniqKey="Esper F">F. Esper</name></author><author><name sortKey="Shapiro, E D" uniqKey="Shapiro E">E.D. Shapiro</name></author><author><name sortKey="Weibel, C" uniqKey="Weibel C">C. Weibel</name></author><author><name sortKey="Ferguson, D" uniqKey="Ferguson D">D. Ferguson</name></author><author><name sortKey="Landry, M L" uniqKey="Landry M">M.L. Landry</name></author><author><name sortKey="Kahn, J S" uniqKey="Kahn J">J.S. Kahn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ebihara, T" uniqKey="Ebihara T">T. Ebihara</name></author><author><name sortKey="Endo, R" uniqKey="Endo R">R. Endo</name></author><author><name sortKey="Ma, X" uniqKey="Ma X">X. Ma</name></author><author><name sortKey="Ishiguro, N" uniqKey="Ishiguro N">N. Ishiguro</name></author><author><name sortKey="Kikuta, H" uniqKey="Kikuta H">H. Kikuta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shimizu, C" uniqKey="Shimizu C">C. Shimizu</name></author><author><name sortKey="Shike, H" uniqKey="Shike H">H. Shike</name></author><author><name sortKey="Baker, S C" uniqKey="Baker S">S.C. Baker</name></author><author><name sortKey="Garcia, F" uniqKey="Garcia F">F. Garcia</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Kuijpers, T W" uniqKey="Kuijpers T">T.W. Kuijpers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, L Y" uniqKey="Chang L">L.Y. Chang</name></author><author><name sortKey="Chiang, B L" uniqKey="Chiang B">B.L. Chiang</name></author><author><name sortKey="Kao, C L" uniqKey="Kao C">C.L. Kao</name></author><author><name sortKey="Wu, M H" uniqKey="Wu M">M.H. Wu</name></author><author><name sortKey="Chen, P J" uniqKey="Chen P">P.J. Chen</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B. Berkhout</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bastien, N" uniqKey="Bastien N">N. Bastien</name></author><author><name sortKey="Anderson, K" uniqKey="Anderson K">K. Anderson</name></author><author><name sortKey="Hart, L" uniqKey="Hart L">L. Hart</name></author><author><name sortKey="Van Caeseele, P" uniqKey="Van Caeseele P">P. Van Caeseele</name></author><author><name sortKey="Brandt, K" uniqKey="Brandt K">K. Brandt</name></author><author><name sortKey="Milley, D" uniqKey="Milley D">D. Milley</name></author><author><name sortKey="Hatchette, T" uniqKey="Hatchette T">T. Hatchette</name></author><author><name sortKey="Weiss, E C" uniqKey="Weiss E">E.C. Weiss</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moes, E" uniqKey="Moes E">E. Moes</name></author><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L. Vijgen</name></author><author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E. Keyaerts</name></author><author><name sortKey="Zlateva, K" uniqKey="Zlateva K">K. Zlateva</name></author><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author><author><name sortKey="Maes, P" uniqKey="Maes P">P. Maes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Hartwig, N G" uniqKey="Hartwig N">N.G. Hartwig</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Niemeyer, B" uniqKey="Niemeyer B">B. Niemeyer</name></author><author><name sortKey="De Jong, J C" uniqKey="De Jong J">J.C. de Jong</name></author><author><name sortKey="Simon, J H" uniqKey="Simon J">J.H. Simon</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sharp, P M" uniqKey="Sharp P">P.M. Sharp</name></author><author><name sortKey="Rogers, M S" uniqKey="Rogers M">M.S. Rogers</name></author><author><name sortKey="Mcconnell, D J" uniqKey="Mcconnell D">D.J. McConnell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="Lavazza, A" uniqKey="Lavazza A">A. Lavazza</name></author><author><name sortKey="Matiz, K" uniqKey="Matiz K">K. Matiz</name></author><author><name sortKey="Horzinek, M C" uniqKey="Horzinek M">M.C. Horzinek</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, A J" uniqKey="Drummond A">A.J. Drummond</name></author><author><name sortKey="Nicholls, G K" uniqKey="Nicholls G">G.K. Nicholls</name></author><author><name sortKey="Rodrigo, A G" uniqKey="Rodrigo A">A.G. Rodrigo</name></author><author><name sortKey="Solomon, W" uniqKey="Solomon W">W. Solomon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goode, M" uniqKey="Goode M">M. Goode</name></author><author><name sortKey="Rodrigo, A G" uniqKey="Rodrigo A">A.G. Rodrigo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, A" uniqKey="Drummond A">A. Drummond</name></author><author><name sortKey="Forsberg, R" uniqKey="Forsberg R">R. Forsberg</name></author><author><name sortKey="Rodrigo, A G" uniqKey="Rodrigo A">A.G. Rodrigo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, A" uniqKey="Drummond A">A. Drummond</name></author><author><name sortKey="Rodrigo, A G" uniqKey="Rodrigo A">A.G. Rodrigo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author><author><name sortKey="Keck, J G" uniqKey="Keck J">J.G. Keck</name></author><author><name sortKey="Egbert, J" uniqKey="Egbert J">J. Egbert</name></author><author><name sortKey="Leibowitz, J L" uniqKey="Leibowitz J">J.L. Leibowitz</name></author><author><name sortKey="Stohlman, S A" uniqKey="Stohlman S">S.A. Stohlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author><author><name sortKey="Keck, J G" uniqKey="Keck J">J.G. Keck</name></author><author><name sortKey="Stohlman, S A" uniqKey="Stohlman S">S.A. Stohlman</name></author><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Stohlman, S A" uniqKey="Stohlman S">S.A. Stohlman</name></author><author><name sortKey="Razavi, M K" uniqKey="Razavi M">M.K. Razavi</name></author><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keck, J G" uniqKey="Keck J">J.G. Keck</name></author><author><name sortKey="Matsushima, G K" uniqKey="Matsushima G">G.K. Matsushima</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author><author><name sortKey="Fleming, J O" uniqKey="Fleming J">J.O. Fleming</name></author><author><name sortKey="Vannier, D M" uniqKey="Vannier D">D.M. Vannier</name></author><author><name sortKey="Stohlman, S A" uniqKey="Stohlman S">S.A. Stohlman</name></author><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kottier, S A" uniqKey="Kottier S">S.A. Kottier</name></author><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name></author><author><name sortKey="Britton, P" uniqKey="Britton P">P. Britton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jia, W" uniqKey="Jia W">W. Jia</name></author><author><name sortKey="Karaca, K" uniqKey="Karaca K">K. Karaca</name></author><author><name sortKey="Parrish, C R" uniqKey="Parrish C">C.R. Parrish</name></author><author><name sortKey="Naqi, S A" uniqKey="Naqi S">S.A. Naqi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kusters, J G" uniqKey="Kusters J">J.G. Kusters</name></author><author><name sortKey="Jager, E J" uniqKey="Jager E">E.J. Jager</name></author><author><name sortKey="Niesters, H G" uniqKey="Niesters H">H.G. Niesters</name></author><author><name sortKey="Van Der Zeijst, B A" uniqKey="Van Der Zeijst B">B.A. van der Zeijst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Junker, D" uniqKey="Junker D">D. Junker</name></author><author><name sortKey="Collisson, E W" uniqKey="Collisson E">E.W. Collisson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herrewegh, A A" uniqKey="Herrewegh A">A.A. Herrewegh</name></author><author><name sortKey="Smeenk, I" uniqKey="Smeenk I">I. Smeenk</name></author><author><name sortKey="Horzinek, M C" uniqKey="Horzinek M">M.C. Horzinek</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L. Kaiser</name></author><author><name sortKey="Regamey, N" uniqKey="Regamey N">N. Regamey</name></author><author><name sortKey="Roiha, H" uniqKey="Roiha H">H. Roiha</name></author><author><name sortKey="Deffernez, C" uniqKey="Deffernez C">C. Deffernez</name></author><author><name sortKey="Frey, U" uniqKey="Frey U">U. Frey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bastien, N" uniqKey="Bastien N">N. Bastien</name></author><author><name sortKey="Robinson, J L" uniqKey="Robinson J">J.L. Robinson</name></author><author><name sortKey="Tse, A" uniqKey="Tse A">A. Tse</name></author><author><name sortKey="Lee, B E" uniqKey="Lee B">B.E. Lee</name></author><author><name sortKey="Hart, L" uniqKey="Hart L">L. Hart</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vabret, A" uniqKey="Vabret A">A. Vabret</name></author><author><name sortKey="Mourez, T" uniqKey="Mourez T">T. Mourez</name></author><author><name sortKey="Dina, J" uniqKey="Dina J">J. Dina</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Gouarin, S" uniqKey="Gouarin S">S. Gouarin</name></author><author><name sortKey="Petitjean, J" uniqKey="Petitjean J">J. Petitjean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suzuki, A" uniqKey="Suzuki A">A. Suzuki</name></author><author><name sortKey="Okamoto, M" uniqKey="Okamoto M">M. Okamoto</name></author><author><name sortKey="Ohmi, A" uniqKey="Ohmi A">A. Ohmi</name></author><author><name sortKey="Watanabe, O" uniqKey="Watanabe O">O. Watanabe</name></author><author><name sortKey="Miyabayashi, S" uniqKey="Miyabayashi S">S. Miyabayashi</name></author><author><name sortKey="Nishimura, H" uniqKey="Nishimura H">H. Nishimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Risco, C" uniqKey="Risco C">C. Risco</name></author><author><name sortKey="Anton, I M" uniqKey="Anton I">I.M. Anton</name></author><author><name sortKey="Sune, C" uniqKey="Sune C">C. Sune</name></author><author><name sortKey="Pedregosa, A M" uniqKey="Pedregosa A">A.M. Pedregosa</name></author><author><name sortKey="Martin Alonso, J M" uniqKey="Martin Alonso J">J.M. Martin-Alonso</name></author><author><name sortKey="Parra, F" uniqKey="Parra F">F. Parra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanchez, C M" uniqKey="Sanchez C">C.M. Sanchez</name></author><author><name sortKey="Gebauer, F" uniqKey="Gebauer F">F. Gebauer</name></author><author><name sortKey="Sune, C" uniqKey="Sune C">C. Sune</name></author><author><name sortKey="Mendez, A" uniqKey="Mendez A">A. Mendez</name></author><author><name sortKey="Dopazo, J" uniqKey="Dopazo J">J. Dopazo</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L. Vijgen</name></author><author><name sortKey="Lemey, P" uniqKey="Lemey P">P. Lemey</name></author><author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E. Keyaerts</name></author><author><name sortKey="Van Ranst, M" uniqKey="Van Ranst M">M. Van Ranst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Chu, D K" uniqKey="Chu D">D.K. Chu</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Wong, O K" uniqKey="Wong O">O.K. Wong</name></author><author><name sortKey="Ellis, T M" uniqKey="Ellis T">T.M. Ellis</name></author><author><name sortKey="Leung, Y H" uniqKey="Leung Y">Y.H. Leung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L. Vijgen</name></author><author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E. Keyaerts</name></author><author><name sortKey="Moes, E" uniqKey="Moes E">E. Moes</name></author><author><name sortKey="Thoelen, I" uniqKey="Thoelen I">I. Thoelen</name></author><author><name sortKey="Wollants, E" uniqKey="Wollants E">E. Wollants</name></author><author><name sortKey="Lemey, P" uniqKey="Lemey P">P. Lemey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L. Vijgen</name></author><author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E. Keyaerts</name></author><author><name sortKey="Lemey, P" uniqKey="Lemey P">P. Lemey</name></author><author><name sortKey="Maes, P" uniqKey="Maes P">P. Maes</name></author><author><name sortKey="Van Reeth, K" uniqKey="Van Reeth K">K. Van Reeth</name></author><author><name sortKey="Nauwynck, H" uniqKey="Nauwynck H">H. Nauwynck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M. Chu</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Tsoi, H W" uniqKey="Tsoi H">H.W. Tsoi</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Yip, C C" uniqKey="Yip C">C.C. Yip</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author><author><name sortKey="Tsoi, H W" uniqKey="Tsoi H">H.W. Tsoi</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brockway, S M" uniqKey="Brockway S">S.M. Brockway</name></author><author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Graham, R L" uniqKey="Graham R">R.L. Graham</name></author><author><name sortKey="Sims, A C" uniqKey="Sims A">A.C. Sims</name></author><author><name sortKey="Brockway, S M" uniqKey="Brockway S">S.M. Brockway</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shi, S T" uniqKey="Shi S">S.T. Shi</name></author><author><name sortKey="Schiller, J J" uniqKey="Schiller J">J.J. Schiller</name></author><author><name sortKey="Kanjanahaluethai, A" uniqKey="Kanjanahaluethai A">A. Kanjanahaluethai</name></author><author><name sortKey="Baker, S C" uniqKey="Baker S">S.C. Baker</name></author><author><name sortKey="Oh, J W" uniqKey="Oh J">J.W. Oh</name></author><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tijms, M A" uniqKey="Tijms M">M.A. Tijms</name></author><author><name sortKey="Van Dinten, L C" uniqKey="Van Dinten L">L.C. van Dinten</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hofmann, H" uniqKey="Hofmann H">H. Hofmann</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Geier, M" uniqKey="Geier M">M. Geier</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B. Berkhout</name></author><author><name sortKey="Pohlmann, S" uniqKey="Pohlmann S">S. Pohlmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lole, K S" uniqKey="Lole K">K.S. Lole</name></author><author><name sortKey="Bollinger, R C" uniqKey="Bollinger R">R.C. Bollinger</name></author><author><name sortKey="Paranjape, R S" uniqKey="Paranjape R">R.S. Paranjape</name></author><author><name sortKey="Gadkari, D" uniqKey="Gadkari D">D. Gadkari</name></author><author><name sortKey="Kulkarni, S S" uniqKey="Kulkarni S">S.S. Kulkarni</name></author><author><name sortKey="Novak, N G" uniqKey="Novak N">N.G. Novak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nielsen, R" uniqKey="Nielsen R">R. Nielsen</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Mol Biol</journal-id><journal-id journal-id-type="iso-abbrev">J. Mol. Biol</journal-id><journal-title-group><journal-title>Journal of Molecular Biology</journal-title></journal-title-group><issn pub-type="ppub">0022-2836</issn><issn pub-type="epub">1089-8638</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">17054987</article-id><article-id pub-id-type="pmc">7094706</article-id><article-id pub-id-type="publisher-id">S0022-2836(06)01298-8</article-id><article-id pub-id-type="doi">10.1016/j.jmb.2006.09.074</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Mosaic Structure of Human Coronavirus NL63, One Thousand Years of Evolution</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pyrc</surname><given-names>Krzysztof</given-names></name><email>k.a.pyrc@amc.uva.nl</email><xref rid="aff1" ref-type="aff">1</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Dijkman</surname><given-names>Ronald</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Lea</given-names></name><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Jebbink</surname><given-names>Maarten F.</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Ross</surname><given-names>Howard A.</given-names></name><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Berkhout</surname><given-names>Ben</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>van der Hoek</surname><given-names>Lia</given-names></name><email>c.m.vanderhoek@amc.uva.nl</email><xref rid="aff1" ref-type="aff">1</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands</aff><aff id="aff2"><label>2</label>School of Biological Sciences and Bioinformatics Institute, University of Auckland, Private Bag 92019, Auckland, New Zealand</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding authors. <email>k.a.pyrc@amc.uva.nl</email><email>c.m.vanderhoek@amc.uva.nl</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>3</day><month>10</month><year>2006</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>15</day><month>12</month><year>2006</year></pub-date><pub-date pub-type="epub"><day>3</day><month>10</month><year>2006</year></pub-date><volume>364</volume><issue>5</issue><fpage>964</fpage><lpage>973</lpage><history><date date-type="received"><day>25</day><month>8</month><year>2006</year></date><date date-type="rev-recd"><day>24</day><month>9</month><year>2006</year></date><date date-type="accepted"><day>25</day><month>9</month><year>2006</year></date></history><permissions><copyright-statement>Copyright © 2006 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2006</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Before the SARS outbreak only two human coronaviruses (HCoV) were known: HCoV-OC43 and HCoV-229E. With the discovery of SARS-CoV in 2003, a third family member was identified. Soon thereafter, we described the fourth human coronavirus (HCoV-NL63), a virus that has spread worldwide and is associated with croup in children. We report here the complete genome sequence of two HCoV-NL63 clinical isolates, designated Amsterdam 57 and Amsterdam 496. The genomes are 27,538 and 27,550 nucleotides long, respectively, and share the same genome organization. We identified two variable regions, one within the 1a and one within the S gene, whereas the 1b and N genes were most conserved. Phylogenetic analysis revealed that HCoV-NL63 genomes have a mosaic structure with multiple recombination sites. Additionally, employing three different algorithms, we assessed the evolutionary rate for the S gene of group Ib coronaviruses to be ∼ 3 × 10<sup>− 4</sup> substitutions per site per year. Using this evolutionary rate we determined that HCoV-NL63 diverged in the 11th century from its closest relative HCoV-229E.</p></abstract><kwd-group><title>Abbreviations used</title><kwd>aa, amino acid residue(s)</kwd><kwd>RdRp, RNA-dependent RNA polymerase</kwd><kwd>ACE, angiotensin converting enzyme</kwd></kwd-group><kwd-group><title>Keywords</title><kwd>coronavirus</kwd><kwd>HCoV-NL63</kwd><kwd>recombination</kwd><kwd>evolution</kwd><kwd>molecular clock</kwd></kwd-group></article-meta><notes><p>Edited by J. Karn</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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