SrasV1, Pmc, Curation, bibRecord, 001028

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

Identifieur interne : 001028 ( Pmc/Curation ); précédent : 001027; suivant : 001029

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

Source :

Genomics, Proteomics & Bioinformatics [ 1672-0229 ] ; 2003.

RBID : PMC:5172421

Abstract

The Coronaviridae family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172421

DOI: 10.1016/S1672-0229(03)01018-0
PubMed: 15626344
PubMed Central: 5172421

Links toward previous steps (curation, corpus...)

to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001028

Links to Exploration step

PMC:5172421

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV</title>
<author><name sortKey="Wang, Jingqiang" sort="Wang, Jingqiang" uniqKey="Wang J" first="Jingqiang" last="Wang">Jingqiang Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ji, Jia" sort="Ji, Jia" uniqKey="Ji J" first="Jia" last="Ji">Jia Ji</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ye, Jia" sort="Ye, Jia" uniqKey="Ye J" first="Jia" last="Ye">Jia Ye</name>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhao, Xiaoqian" sort="Zhao, Xiaoqian" uniqKey="Zhao X" first="Xiaoqian" last="Zhao">Xiaoqian Zhao</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hu, Jianfei" sort="Hu, Jianfei" uniqKey="Hu J" first="Jianfei" last="Hu">Jianfei Hu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0015">College of Life Sciences, Peking University, Beijing 100871, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sun, Min" sort="Sun, Min" uniqKey="Sun M" first="Min" last="Sun">Min Sun</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zeng, Haipan" sort="Zeng, Haipan" uniqKey="Zeng H" first="Haipan" last="Zeng">Haipan Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tan, Xuehai" sort="Tan, Xuehai" uniqKey="Tan X" first="Xuehai" last="Tan">Xuehai Tan</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bi, Shengli" sort="Bi, Shengli" uniqKey="Bi S" first="Shengli" last="Bi">Shengli Bi</name>
<affiliation wicri:level="1"><nlm:aff id="aff0020">Center of Disease Control and Prevention, Beijing 100050, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center of Disease Control and Prevention, Beijing 100050</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">15626344</idno>
<idno type="pmc">5172421</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172421</idno>
<idno type="RBID">PMC:5172421</idno>
<idno type="doi">10.1016/S1672-0229(03)01018-0</idno>
<date when="2003">2003</date>
<idno type="wicri:Area/Pmc/Corpus">001028</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001028</idno>
<idno type="wicri:Area/Pmc/Curation">001028</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001028</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV</title>
<author><name sortKey="Wang, Jingqiang" sort="Wang, Jingqiang" uniqKey="Wang J" first="Jingqiang" last="Wang">Jingqiang Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ji, Jia" sort="Ji, Jia" uniqKey="Ji J" first="Jia" last="Ji">Jia Ji</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ye, Jia" sort="Ye, Jia" uniqKey="Ye J" first="Jia" last="Ye">Jia Ye</name>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhao, Xiaoqian" sort="Zhao, Xiaoqian" uniqKey="Zhao X" first="Xiaoqian" last="Zhao">Xiaoqian Zhao</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hu, Jianfei" sort="Hu, Jianfei" uniqKey="Hu J" first="Jianfei" last="Hu">Jianfei Hu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0015">College of Life Sciences, Peking University, Beijing 100871, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sun, Min" sort="Sun, Min" uniqKey="Sun M" first="Min" last="Sun">Min Sun</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zeng, Haipan" sort="Zeng, Haipan" uniqKey="Zeng H" first="Haipan" last="Zeng">Haipan Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tan, Xuehai" sort="Tan, Xuehai" uniqKey="Tan X" first="Xuehai" last="Tan">Xuehai Tan</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bi, Shengli" sort="Bi, Shengli" uniqKey="Bi S" first="Shengli" last="Bi">Shengli Bi</name>
<affiliation wicri:level="1"><nlm:aff id="aff0020">Center of Disease Control and Prevention, Beijing 100050, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center of Disease Control and Prevention, Beijing 100050</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Genomics, Proteomics & Bioinformatics</title>
<idno type="ISSN">1672-0229</idno>
<idno type="eISSN">2210-3244</idno>
<imprint><date when="2003">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The <italic>Coronaviridae</italic>
 family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hiscox, J A" uniqKey="Hiscox J">J.A. Hiscox</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wootton, S K" uniqKey="Wootton S">S.K. Wootton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laity, J H" uniqKey="Laity J">J.H. Laity</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R. Molenkamp</name>
</author>
<author><name sortKey="Spaan, W J M" uniqKey="Spaan W">W.J.M. Spaan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ding, P" uniqKey="Ding P">P. Ding</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Narayahan, K" uniqKey="Narayahan K">K. Narayahan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caceres, J F" uniqKey="Caceres J">J.F. Cáceres</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furuyama, S" uniqKey="Furuyama S">S. Furuyama</name>
</author>
<author><name sortKey="Bruzik, J P" uniqKey="Bruzik J">J.P. Bruzik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wurm, T" uniqKey="Wurm T">T. Wurm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kolaskar, A S" uniqKey="Kolaskar A">A.S. Kolaskar</name>
</author>
<author><name sortKey="Tongaonkar, P C" uniqKey="Tongaonkar P">P.C. Tongaonkar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Daginakatte, C G" uniqKey="Daginakatte C">C.G. Daginakatte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhu, Q Y" uniqKey="Zhu Q">Q.Y. Zhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E.D. Qin</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Genomics Proteomics Bioinformatics</journal-id>
<journal-id journal-id-type="iso-abbrev">Genomics Proteomics Bioinformatics</journal-id>
<journal-title-group><journal-title>Genomics, Proteomics & Bioinformatics</journal-title>
</journal-title-group>
<issn pub-type="ppub">1672-0229</issn>
<issn pub-type="epub">2210-3244</issn>
<publisher><publisher-name>Elsevier</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15626344</article-id>
<article-id pub-id-type="pmc">5172421</article-id>
<article-id pub-id-type="publisher-id">S1672-0229(03)01018-0</article-id>
<article-id pub-id-type="doi">10.1016/S1672-0229(03)01018-0</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Invited Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Jingqiang</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="aff0010" ref-type="aff">2</xref>
<xref rid="fn1" ref-type="fn">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ji</surname>
<given-names>Jia</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="fn1" ref-type="fn">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ye</surname>
<given-names>Jia</given-names>
</name>
<xref rid="aff0010" ref-type="aff">2</xref>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="fn1" ref-type="fn">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhao</surname>
<given-names>Xiaoqian</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="fn1" ref-type="fn">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wen</surname>
<given-names>Jie</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Wei</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>Jianfei</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="aff0015" ref-type="aff">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Dawei</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Min</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zeng</surname>
<given-names>Haipan</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>Yongwu</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tian</surname>
<given-names>Xiangjun</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="aff0010" ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tan</surname>
<given-names>Xuehai</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xu</surname>
<given-names>Ningzhi</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zeng</surname>
<given-names>Changqing</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Jian</given-names>
</name>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="aff0010" ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bi</surname>
<given-names>Shengli</given-names>
</name>
<xref rid="aff0020" ref-type="aff">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Huanming</given-names>
</name>
<email>yanghm@genomics.org.cn</email>
<xref rid="aff0005" ref-type="aff">1</xref>
<xref rid="aff0010" ref-type="aff">2</xref>
<xref rid="cor1" ref-type="corresp">#</xref>
</contrib>
</contrib-group>
<aff id="aff0005"><label>1</label>
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</aff>
<aff id="aff0010"><label>2</label>
James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</aff>
<aff id="aff0015"><label>3</label>
College of Life Sciences, Peking University, Beijing 100871, China</aff>
<aff id="aff0020"><label>4</label>
Center of Disease Control and Prevention, Beijing 100050, China</aff>
<author-notes><corresp id="cor1"><label>#</label>
Corresponding author. <email>yanghm@genomics.org.cn</email>
</corresp>
<fn id="fn1"><label>*</label>
<p id="ntp0035">These authors contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="pmc-release"><day>28</day>
<month>11</month>
<year>2016</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
      <pub-date pub-type="ppub"><month>5</month>
<year>2003</year>
</pub-date>
<pub-date pub-type="epub"><day>28</day>
<month>11</month>
<year>2016</year>
</pub-date>
<volume>1</volume>
<issue>2</issue>
<fpage>145</fpage>
<lpage>154</lpage>
<permissions><copyright-statement>.</copyright-statement>
<copyright-year>2003</copyright-year>
<copyright-holder>Beijing Institute of Genomics, the Chinese Academy of Sciences and the Genetics Society of China</copyright-holder>
<license license-type="CC BY-NC-ND" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/"><license-p>This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).</license-p>
</license>
</permissions>
<abstract id="ab0005"><p>The <italic>Coronaviridae</italic>
 family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.</p>
</abstract>
<kwd-group id="keys0005"><title>Key words</title>
<kwd>SARS-CoV</kwd>
<kwd>nucleoprotein</kwd>
<kwd>phosphorylation</kwd>
<kwd>SR-rich region</kwd>
<kwd>antigenic sites</kwd>
</kwd-group>
</article-meta>
</front>
<floats-group><fig id="f0005"><label>Fig. 1</label>
<caption><p>The predicted distributions of GC content (A), electric charge (B), hydrophobicity (C) and secondary structure (D) in the N protein of SARS-CoV.</p>
</caption>
<alt-text id="at0005">Fig. 1</alt-text>
<graphic xlink:href="gr1"></graphic>
</fig>
<fig id="f0010"><label>Fig. 2</label>
<caption><p>The predicted phosphorylation sites on the N protein. We identified 33 potential phosphorylation sites in the N protein, including 22 serines, 8 threonines and 3 tyrosines. The average score of serines are significantly higher than that of the other two. The phosphorylation sites concentrate in the middle of the N protein.</p>
</caption>
<alt-text id="at0010">Fig. 2</alt-text>
<graphic xlink:href="gr2"></graphic>
</fig>
<fig id="f0015"><label>Fig. 3</label>
<caption><p>The similarity chart of the N protein. Based on multi-alignment of totally nineteen coronavirus N proteins, two conserved regions were found around a.a. 81-140 and a.a. 270-320 (amino acid positions are all referred to the N protein of SARS-CoV, Isolate BJ01). The arrow indicates the most conserved domain, and its sequence and amino acid position are given. In contrast, the two termini of the N protein are more variable, particularly the C- terminal. The figure was generated by Plotcon in the EMBOSS package (<ext-link ext-link-type="uri" xlink:href="http://www.hgmp.mrc.ac.uk/Software/EMBOSS/" id="ir0005">http://www.hgmp.mrc.ac.uk/Software/EMBOSS/</ext-link>
).</p>
</caption>
<alt-text id="at0015">Fig. 3</alt-text>
<graphic xlink:href="gr3"></graphic>
</fig>
<fig id="f0020"><label>Fig. 4</label>
<caption><p>The possible antigenic sites of the N protein. We have predicted sixteen antigenic sites of the N protein, and found they are clustering in the middle and the C-terminal. There is a strong antigenic site (TALALLLLDR) located around Codons 218-227. In addition, another three strong antigenic sites are detected around Codons 156-166 (AATVLQLPQGT), Codons 347-363 (FKDNVILLNKHIDAYKT) and Codons 389-398 (KQPTVTLLPA).</p>
</caption>
<alt-text id="at0020">Fig. 4</alt-text>
<graphic xlink:href="gr4"></graphic>
</fig>
<fig id="f0025"><label>Fig. 5</label>
<caption><p>The phylogenetic tree based on nineteen coronavirus N proteins. It reveals that SARS-CoV is closer to Group 2 than to Groups 1 and 3. Abbreviations: AIBV: avian infectious bronchitis virus; BCoV: bovine coronavirus; CCoV: canine coronavirus; CECoV: canine enteric coronavirus; ECoV: equine coronavirus; FCoV: feline coronavirus; FIPV: feline infectious peritonitis virus; HCoV-OC43: human coronavirus strain OC43; HCoV-229E: human coronavirus strain 229E; MHV: murine hepatitis virus; PEDV: porcine epidemic diarrhea virus; PHEV: porcine hemagglutmating encephalomyelitis virus; PRCoV: porcine respiratory coronavirus; PTGV: transmissible gastroenteritis virus; PV: puffinosis virus; RCoV: rat coronavirus; RSCoV: Rat sialodacryoadenitis coronavirus; SARS-CoV: human severe acute respiratory syndrome-associated coronavirus isolate BJ01; TCoV: turkey coronavirus.</p>
</caption>
<alt-text id="at0025">Fig. 5</alt-text>
<graphic xlink:href="gr5"></graphic>
</fig>
<table-wrap id="t0005" position="float"><label>Table 1</label>
<caption><p>The Amino Acid Composition of the SARS-CoV N Protein</p>
</caption>
<alt-text id="at0030">Table 1</alt-text>
<table frame="hsides" rules="groups"><thead><tr><th></th>
<th align="right">Number</th>
<th align="right">Percentage (%)</th>
</tr>
</thead>
<tbody><tr><td align="center"><bold>Non-polar, Neutral</bold>
</td>
<td></td>
<td></td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center">Ala, A</td>
<td align="right">34</td>
<td align="char">8.06</td>
</tr>
<tr><td align="center">Phe, F</td>
<td align="right">13</td>
<td align="char">3.08</td>
</tr>
<tr><td align="center">Gly, G</td>
<td align="right">45</td>
<td align="char">10.66</td>
</tr>
<tr><td align="center">Ile, I</td>
<td align="right">11</td>
<td align="char">2.61</td>
</tr>
<tr><td align="center">Leu, L</td>
<td align="right">26</td>
<td align="char">6.16</td>
</tr>
<tr><td align="center">Met, M</td>
<td align="right">7</td>
<td align="char">1.66</td>
</tr>
<tr><td align="center">Pro, P</td>
<td align="right">31</td>
<td align="char">7.35</td>
</tr>
<tr><td align="center">Val, V</td>
<td align="right">11</td>
<td align="char">2.61</td>
</tr>
<tr><td align="center">Trp, W</td>
<td align="right">5</td>
<td align="char">1.18</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Total</bold>
</td>
<td align="right">183</td>
<td align="char">43.36</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Polar, Neutral</bold>
</td>
<td></td>
<td></td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center">Cys, C</td>
<td align="right">0</td>
<td align="char">0.00</td>
</tr>
<tr><td align="center">Asn. N</td>
<td align="right">25</td>
<td align="char">5.92</td>
</tr>
<tr><td align="center">Gln, Q</td>
<td align="right">34</td>
<td align="char">8.06</td>
</tr>
<tr><td align="center">Ser, S</td>
<td align="right">35</td>
<td align="char">8.29</td>
</tr>
<tr><td align="center">Thr, T</td>
<td align="right">33</td>
<td align="char">7.82</td>
</tr>
<tr><td align="center">Tyr, Y</td>
<td align="right">11</td>
<td align="char">2.61</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Total</bold>
</td>
<td align="right">138</td>
<td align="char">32.70</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Polar, Positive</bold>
</td>
<td></td>
<td></td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center">His, H</td>
<td align="right">5</td>
<td align="char">1.18</td>
</tr>
<tr><td align="center">Lys, K</td>
<td align="right">29</td>
<td align="char">6.87</td>
</tr>
<tr><td align="center">Arg, R</td>
<td align="right">31</td>
<td align="char">7.35</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Total</bold>
</td>
<td align="right">65</td>
<td align="char">15.40</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Polar, Negative</bold>
</td>
<td></td>
<td></td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center">Asp, D</td>
<td align="right">22</td>
<td align="char">5.21</td>
</tr>
<tr><td align="center">Glu, E</td>
<td align="right">14</td>
<td align="char">3.32</td>
</tr>
<tr><td colspan="3"><hr></hr>
</td>
</tr>
<tr><td align="center"><bold>Total</bold>
</td>
<td align="right">36</td>
<td align="char">8.53</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t0010" position="float"><label>Table 2</label>
<caption><p>The Core Motif of SR-rich Region in the Coronavirus N Protein</p>
</caption>
<alt-text id="at0035">Table 2</alt-text>
<table frame="hsides" rules="groups"><thead><tr><th>Coronavirus</th>
<th>Core motif of SR-rich region in the N protein</th>
</tr>
</thead>
<tbody><tr><td>SARS Coronavirus BJ01</td>
<td><bold>SR</bold>
GGSQAS<bold>SR</bold>
SS<bold>SRSR</bold>
GN<bold>SR</bold>
NSTPGS <bold>SR</bold>
<xref rid="tbl2fnStar" ref-type="table-fn">*</xref>
</td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Murine Hepatitis Virus</td>
<td></td>
</tr>
<tr><td>Puffinosis Virus</td>
<td></td>
</tr>
<tr><td>Rat Sialodacryoadenitis Coronavirus</td>
<td><bold>SR</bold>
SG<bold>SR</bold>
SQ<bold>SR</bold>
</td>
</tr>
<tr><td>Rat Coronavirus</td>
<td></td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Equine Coronavirus</td>
<td></td>
</tr>
<tr><td>Bovine Coronavirus</td>
<td></td>
</tr>
<tr><td>Porcine Hemagglutinating Encephalomyelitis Virus</td>
<td><bold>SR</bold>
ST<bold>SR</bold>
[A/T]<xref rid="tbl2fn1" ref-type="table-fn">#</xref>
[S/P][<bold>S/N</bold>
]<bold>R</bold>
A[S/P]SAG<bold>SR</bold>
(<bold>SR</bold>
)<xref rid="tbl2fn2" ref-type="table-fn">†</xref>
</td>
</tr>
<tr><td>HCoV-OC43</td>
<td></td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Turkey Coronavirus</td>
<td></td>
</tr>
<tr><td>Avian Infectious Bronchitis Virus</td>
<td><bold>S</bold>
(T)<bold>R</bold>
AP<bold>SR</bold>
EG <bold>SR</bold>
{Xn}<xref rid="tbl2fn3" ref-type="table-fn">‡</xref>
<bold>SR</bold>
</td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Porcine Respiratory Coronavirus</td>
<td></td>
</tr>
<tr><td>Transmissible Gastroenteritis Virus</td>
<td></td>
</tr>
<tr><td>Canine Enteric Coronavirus</td>
<td><bold>SR</bold>
DN<bold>SRS</bold>
[<bold>R/P</bold>
] SQ<bold>SRS</bold>
[<bold>R/Q</bold>
]<bold>SR</bold>
NRSQ<bold>SR</bold>
{Xn}<bold>SR</bold>
</td>
</tr>
<tr><td>Canine Coronavirus</td>
<td></td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Feline Infectious Peritonitis Virus</td>
<td><bold>SR</bold>
NN <bold>SR</bold>
SGSQ<bold>SR</bold>
SV<bold>SR</bold>
 NRSQ{Xn}<bold>SR</bold>
{Xn}<bold>SR</bold>
</td>
</tr>
<tr><td>Feline Coronavirus</td>
<td></td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Human Coronavirus 229E</td>
<td><bold>SR</bold>
AP<bold>SR</bold>
SQ<bold>SR</bold>
SQ <bold>SR</bold>
{Xn}<bold>SR</bold>
{Xn}<bold>SR</bold>
</td>
</tr>
<tr><td colspan="2"><hr></hr>
</td>
</tr>
<tr><td>Porcine Epidemic Diarrhea Virus</td>
<td><bold>SR</bold>
AN<bold>SRSRSR</bold>
{Xn} <bold>SR</bold>
{Xn}<bold>SR</bold>
{Xn}<bold>SR</bold>
{Xn}<bold>SR</bold>
</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="tbl2fnStar"><label>*</label>
<p id="ntp0005">Bold letter indicates the marker SR. Normal letter indicates the amino acid between two SRs.</p>
</fn>
</table-wrap-foot>
<table-wrap-foot><fn id="tbl2fn1"><label>#</label>
<p id="ntp0010">Square brackets indicate this position may be occupied by one of the amino acids in them.</p>
</fn>
</table-wrap-foot>
<table-wrap-foot><fn id="tbl2fn2"><label>†</label>
<p id="ntp0015">Round brackets indicate the amino acids in them may occur in some viruses while may not in other viruses.</p>
</fn>
</table-wrap-foot>
<table-wrap-foot><fn id="tbl2fn3"><label>‡</label>
<p id="ntp0020">Curly brackets indicate amino acids between SRs, X indicates any amino acid, and subscript letter n indicates the number of amino acids between SRs.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<table-wrap id="t0015" position="float"><label>Table 3</label>
<caption><p>The Predicted Antigenic Sites on the SARS-CoV N Protein</p>
</caption>
<alt-text id="at0040">Table 3</alt-text>
<table frame="hsides" rules="groups"><thead><tr><th align="center">No.</th>
<th align="center">Start Position<xref rid="tbl3fnStar" ref-type="table-fn">*</xref>
</th>
<th align="center">Sequence</th>
<th align="center">End Position</th>
</tr>
</thead>
<tbody><tr><td align="center">1</td>
<td align="center">52</td>
<td align="center">SWFTALTQ</td>
<td align="center">59</td>
</tr>
<tr><td align="center">2</td>
<td align="center">69</td>
<td align="center">RGQGVPI</td>
<td align="center">75</td>
</tr>
<tr><td align="center">3</td>
<td align="center">83</td>
<td align="center">DQIGYYR</td>
<td align="center">89</td>
</tr>
<tr><td align="center">4</td>
<td align="center">106</td>
<td align="center">SPRWYFYYLG</td>
<td align="center">115</td>
</tr>
<tr><td align="center">5</td>
<td align="center">118</td>
<td align="center">PEASLPY</td>
<td align="center">124</td>
</tr>
<tr><td align="center">6</td>
<td align="center">130</td>
<td align="center">GIVWVAT</td>
<td align="center">136</td>
</tr>
<tr><td align="center">7</td>
<td align="center">156</td>
<td align="center">AATVLQLPQGT</td>
<td align="center">166</td>
</tr>
<tr><td align="center">8</td>
<td align="center">218</td>
<td align="center">TALALLLLDR</td>
<td align="center">227</td>
</tr>
<tr><td align="center">9</td>
<td align="center">229</td>
<td align="center">NQLESKVSG</td>
<td align="center">237</td>
</tr>
<tr><td align="center">10</td>
<td align="center">243</td>
<td align="center">QGQTVTK</td>
<td align="center">249</td>
</tr>
<tr><td align="center">11</td>
<td align="center">267</td>
<td align="center">KQYNVTQ</td>
<td align="center">273</td>
</tr>
<tr><td align="center">12</td>
<td align="center">299</td>
<td align="center">YKHWPQIAQFAPSASAF</td>
<td align="center">315</td>
</tr>
<tr><td align="center">13</td>
<td align="center">323</td>
<td align="center">MEVTPSGTWLTYHGAIK</td>
<td align="center">339</td>
</tr>
<tr><td align="center">14</td>
<td align="center">347</td>
<td align="center">FKDNVILLNKHIDAYKT</td>
<td align="center">363</td>
</tr>
<tr><td align="center">15</td>
<td align="center">379</td>
<td align="center">EAQPLPQ</td>
<td align="center">385</td>
</tr>
<tr><td align="center">16</td>
<td align="center">389</td>
<td align="center">KQPTVTLLPA</td>
<td align="center">398</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="tbl3fnStar"><label>*</label>
<p id="ntp0025">amino acid position in the SARS-CoV N protein (BJ01)</p>
</fn>
</table-wrap-foot>
</table-wrap>
<table-wrap id="t0020" position="float"><label>Table 4</label>
<caption><p>The Synthesized Peptides Representing the N Protein of SARS-CoV and Their ELISA Result</p>
</caption>
<alt-text id="at0045">Table 4</alt-text>
<table frame="hsides" rules="groups"><thead><tr><th align="center">Peptides Number</th>
<th align="center">Start Position<xref rid="tbl4fnStar" ref-type="table-fn">*</xref>
</th>
<th align="center">Sequence</th>
<th align="center">End Position</th>
<th align="center">ELISA Result</th>
</tr>
</thead>
<tbody><tr><td align="center">N1</td>
<td align="center">1</td>
<td>MSDNGPQSNQRSAPRITFGGPTD</td>
<td align="center">23</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N21</td>
<td align="center">21</td>
<td>PTDSTDNNQNGGRNGARPKQRR</td>
<td align="center">42</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N35</td>
<td align="center">35</td>
<td>GARPKQRRPQGLPNNTASWFTA</td>
<td align="center">56</td>
<td align="center">+</td>
</tr>
<tr><td align="center">N99</td>
<td align="center">99</td>
<td>DGKMKELSPRWYFYYLGTGPEA</td>
<td align="center">120</td>
<td align="center">-</td>
</tr>
<tr><td align="center">N161</td>
<td align="center">161</td>
<td>QLPQGTTLPKGFYAEGSRGGSQ</td>
<td align="center">182</td>
<td align="center">+++</td>
</tr>
<tr><td align="center">N177</td>
<td align="center">177</td>
<td>SRGGSQASSRSSSRSRGNSRNS</td>
<td align="center">198</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N196</td>
<td align="center">196</td>
<td>RNSTPGSSRGNSPARMASGGGE</td>
<td align="center">217</td>
<td align="center">-</td>
</tr>
<tr><td align="center">N215</td>
<td align="center">215</td>
<td>GGETALALLLLDRLNQLESKVSGKG</td>
<td align="center">239</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N245</td>
<td align="center">245</td>
<td>QTVTKKSAAEASKKPRQKRTATKQ</td>
<td align="center">268</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N258</td>
<td align="center">258</td>
<td>KPRQKRTATKQYNVTQAFGRRG</td>
<td align="center">279</td>
<td align="center">+</td>
</tr>
<tr><td align="center">N355</td>
<td align="center">355</td>
<td>NKHIDAYKTFPPTEPKKDKKKK</td>
<td align="center">376</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N371</td>
<td align="center">371</td>
<td>KDKKKKTDEAQPLPQRQKKQ</td>
<td align="center">390</td>
<td align="center">+++</td>
</tr>
<tr><td align="center">N385</td>
<td align="center">385</td>
<td>QRQKKQPTVTLLPAADMDDFSRQ</td>
<td align="center">407</td>
<td align="center">++</td>
</tr>
<tr><td align="center">N401</td>
<td align="center">401</td>
<td>MDDFSRQLQNSMSGASADSTQA</td>
<td align="center">422</td>
<td align="center">-</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="tbl4fnStar"><label>*</label>
<p id="ntp0030">amino acid position in the SARS-CoV N protein (BJ01)</p>
</fn>
</table-wrap-foot>
</table-wrap>
<table-wrap id="t0025" position="float"><label>Table 5</label>
<caption><p>The Four Substitutions in the N Protein of SARS-CoV</p>
</caption>
<alt-text id="at0050">Table 5</alt-text>
<table frame="hsides" rules="groups"><thead><tr><th align="center">Nt Position in BJ01</th>
<th align="center">a.a. Position in the ORF</th>
<th align="center">a.a. (Ratio)</th>
<th align="center">Synonymous Substitution</th>
</tr>
</thead>
<tbody><tr><td align="center">28,519</td>
<td align="center">140</td>
<td align="center">L(16)/W(1)</td>
<td align="center">No</td>
</tr>
<tr><td align="center">28,520</td>
<td align="center">140</td>
<td align="center">L(17)</td>
<td align="center">Yes</td>
</tr>
<tr><td align="center">28,560</td>
<td align="center">154</td>
<td align="center">N(16)/Y(1)</td>
<td align="center">No</td>
</tr>
<tr><td align="center">28,677</td>
<td align="center">193</td>
<td align="center">G(16)/C(1)</td>
<td align="center">No</td>
</tr>
</tbody>
</table>
</table-wrap>
</floats-group>
</pmc>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001028 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 001028 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Pmc
   |étape=   Curation
   |type=    RBID
   |clé=     PMC:5172421
   |texte=   The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i   -Sk "pubmed:15626344" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki