Ribavirin and interferon-β synergistically inhibit SARS-associated coronavirus replication in animal and human cell lines
Identifieur interne : 000E25 ( Pmc/Curation ); précédent : 000E24; suivant : 000E26Ribavirin and interferon-β synergistically inhibit SARS-associated coronavirus replication in animal and human cell lines
Auteurs : Birgit Morgenstern [Allemagne] ; Martin Michaelis [Allemagne] ; Patrick C. Baer [Allemagne] ; Hans W. Doerr [Allemagne] ; Jindrich Cinatl [Allemagne]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2004.
Abstract
Initial in vitro investigations demonstrated type I interferons (IFNs: IFN-α, IFN-β) to inhibit replication of SARS coronavirus (SARS-CoV), but found the nucleoside analogue ribavirin ineffective in Vero cells. In this report, ribavirin was shown to inhibit SARS-CoV replication in five different cell types of animal or human origin at therapeutically achievable concentrations. Since clinical anti-SARS-CoV activity of type I interferons or ribavirin is limited, we investigated the combination of IFN-β and ribavirin. Determination of the virus yield indicated highly synergistic anti-SARS-CoV action of the combination suggesting the consideration of ribavirin plus IFN-β for the treatment of SARS.
Url:
DOI: 10.1016/j.bbrc.2004.11.128
PubMed: 15607755
PubMed Central: 7092851
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Baer</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Internal Medicine IV, Institute of Nephrologie, Johann Wolfgang Goethe University Frankfurt, 60596 Frankfurt, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Internal Medicine IV, Institute of Nephrologie, Johann Wolfgang Goethe University Frankfurt, 60596 Frankfurt</wicri:regionArea></affiliation></author><author><name sortKey="Doerr, Hans W" sort="Doerr, Hans W" uniqKey="Doerr H" first="Hans W." last="Doerr">Hans W. 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Baer</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Internal Medicine IV, Institute of Nephrologie, Johann Wolfgang Goethe University Frankfurt, 60596 Frankfurt, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Internal Medicine IV, Institute of Nephrologie, Johann Wolfgang Goethe University Frankfurt, 60596 Frankfurt</wicri:regionArea></affiliation></author><author><name sortKey="Doerr, Hans W" sort="Doerr, Hans W" uniqKey="Doerr H" first="Hans W." last="Doerr">Hans W. Doerr</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt</wicri:regionArea></affiliation></author><author><name sortKey="Cinatl, Jindrich" sort="Cinatl, Jindrich" uniqKey="Cinatl J" first="Jindrich" last="Cinatl">Jindrich Cinatl</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biochemical and Biophysical Research Communications</title><idno type="ISSN">0006-291X</idno><idno type="eISSN">1090-2104</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Initial in vitro investigations demonstrated type I interferons (IFNs: IFN-α, IFN-β) to inhibit replication of SARS coronavirus (SARS-CoV), but found the nucleoside analogue ribavirin ineffective in Vero cells. In this report, ribavirin was shown to inhibit SARS-CoV replication in five different cell types of animal or human origin at therapeutically achievable concentrations. Since clinical anti-SARS-CoV activity of type I interferons or ribavirin is limited, we investigated the combination of IFN-β and ribavirin. Determination of the virus yield indicated highly synergistic anti-SARS-CoV action of the combination suggesting the consideration of ribavirin plus IFN-β for the treatment of SARS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Vander Werf, S" uniqKey="Vander Werf S">S. vander Werf</name></author><author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. 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Sheng</name></author><author><name sortKey="Hsueh, P R" uniqKey="Hsueh P">P.R. Hsueh</name></author><author><name sortKey="Yang, C F" uniqKey="Yang C">C.F. Yang</name></author><author><name sortKey="Yang, P C" uniqKey="Yang P">P.C. Yang</name></author><author><name sortKey="Chang, S C" uniqKey="Chang S">S.C. Chang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biochem Biophys Res Commun</journal-id><journal-id journal-id-type="iso-abbrev">Biochem. Biophys. Res. Commun</journal-id><journal-title-group><journal-title>Biochemical and Biophysical Research Communications</journal-title></journal-title-group><issn pub-type="ppub">0006-291X</issn><issn pub-type="epub">1090-2104</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15607755</article-id><article-id pub-id-type="pmc">7092851</article-id><article-id pub-id-type="publisher-id">S0006-291X(04)02729-9</article-id><article-id pub-id-type="doi">10.1016/j.bbrc.2004.11.128</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Ribavirin and interferon-β synergistically inhibit SARS-associated coronavirus replication in animal and human cell lines</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Morgenstern</surname><given-names>Birgit</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Michaelis</surname><given-names>Martin</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Baer</surname><given-names>Patrick C.</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Doerr</surname><given-names>Hans W.</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Cinatl</surname><given-names>Jindrich</given-names><suffix>Jr.</suffix></name><email>cinatl@em.uni-frankfurt.de</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">*</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Institute of Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany</aff><aff id="aff2"><label>b</label>Department of Internal Medicine IV, Institute of Nephrologie, Johann Wolfgang Goethe University Frankfurt, 60596 Frankfurt, Germany</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Fax: +49 69 6301 4302 <email>cinatl@em.uni-frankfurt.de</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>8</day><month>12</month><year>2004</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>28</day><month>1</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>8</day><month>12</month><year>2004</year></pub-date><volume>326</volume><issue>4</issue><fpage>905</fpage><lpage>908</lpage><history><date date-type="received"><day>12</day><month>11</month><year>2004</year></date></history><permissions><copyright-statement>Copyright © 2004 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2004</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Initial in vitro investigations demonstrated type I interferons (IFNs: IFN-α, IFN-β) to inhibit replication of SARS coronavirus (SARS-CoV), but found the nucleoside analogue ribavirin ineffective in Vero cells. In this report, ribavirin was shown to inhibit SARS-CoV replication in five different cell types of animal or human origin at therapeutically achievable concentrations. Since clinical anti-SARS-CoV activity of type I interferons or ribavirin is limited, we investigated the combination of IFN-β and ribavirin. Determination of the virus yield indicated highly synergistic anti-SARS-CoV action of the combination suggesting the consideration of ribavirin plus IFN-β for the treatment of SARS.</p></abstract><kwd-group><title>Keywords</title><kwd>Human primary epithelial kidney cells</kwd><kwd>Caco2</kwd><kwd>CL14</kwd><kwd>MA104</kwd><kwd>Vero</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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