Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential
Identifieur interne : 000878 ( Pmc/Curation ); précédent : 000877; suivant : 000879Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential
Auteurs : Wei Lu [République populaire de Chine] ; Xiao-Dong Wu [République populaire de Chine] ; Mu De Shi [République populaire de Chine] ; Rui Fu Yang ; You Yu He ; Chao Bian [République populaire de Chine] ; Tie Liu Shi ; Sheng Yang ; Xue-Liang Zhu [République populaire de Chine] ; Wei-Hong Jiang ; Yi Xue Li ; Lin-Chen Yan [République populaire de Chine] ; Yong Yong Ji [République populaire de Chine] ; Ying Lin [République populaire de Chine] ; Guo-Mei Lin [République populaire de Chine] ; Lin Tian [République populaire de Chine] ; Jin Wang ; Hong Xia Wang ; You Hua Xie [République populaire de Chine] ; Gang Pei [République populaire de Chine] ; Jia Rui Wu [République populaire de Chine] ; Bing Sun [République populaire de Chine]Source :
- Febs Letters [ 0014-5793 ] ; 2005.
Abstract
The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.
Url:
DOI: 10.1016/j.febslet.2005.02.070
PubMed: 15811330
PubMed Central: 7094314
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Division, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>E-institutes of Shanghai Universities Immunology Division</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15811330</idno><idno type="pmc">7094314</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094314</idno><idno type="RBID">PMC:7094314</idno><idno type="doi">10.1016/j.febslet.2005.02.070</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000878</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000878</idno><idno type="wicri:Area/Pmc/Curation">000878</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000878</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential</title><author><name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Xiao Dong" sort="Wu, Xiao Dong" uniqKey="Wu X" first="Xiao-Dong" last="Wu">Xiao-Dong Wu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Mu De" sort="Shi, Mu De" uniqKey="Shi M" first="Mu De" last="Shi">Mu De Shi</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Rui Fu" sort="Yang, Rui Fu" uniqKey="Yang R" first="Rui Fu" last="Yang">Rui Fu Yang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="He, You Yu" sort="He, You Yu" uniqKey="He Y" first="You Yu" last="He">You Yu He</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Bian, Chao" sort="Bian, Chao" uniqKey="Bian C" first="Chao" last="Bian">Chao Bian</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Tie Liu" sort="Shi, Tie Liu" uniqKey="Shi T" first="Tie Liu" last="Shi">Tie Liu Shi</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Yang, Sheng" sort="Yang, Sheng" uniqKey="Yang S" first="Sheng" last="Yang">Sheng Yang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zhu, Xue Liang" sort="Zhu, Xue Liang" uniqKey="Zhu X" first="Xue-Liang" last="Zhu">Xue-Liang Zhu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Wei Hong" sort="Jiang, Wei Hong" uniqKey="Jiang W" first="Wei-Hong" last="Jiang">Wei-Hong Jiang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Li, Yi Xue" sort="Li, Yi Xue" uniqKey="Li Y" first="Yi Xue" last="Li">Yi Xue Li</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Yan, Lin Chen" sort="Yan, Lin Chen" uniqKey="Yan L" first="Lin-Chen" last="Yan">Lin-Chen Yan</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Ji, Yong Yong" sort="Ji, Yong Yong" uniqKey="Ji Y" first="Yong Yong" last="Ji">Yong Yong Ji</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Guo Mei" sort="Lin, Guo Mei" uniqKey="Lin G" first="Guo-Mei" last="Lin">Guo-Mei Lin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Tian, Lin" sort="Tian, Lin" uniqKey="Tian L" first="Lin" last="Tian">Lin Tian</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Wang, Hong Xia" sort="Wang, Hong Xia" uniqKey="Wang H" first="Hong Xia" last="Wang">Hong Xia Wang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Xie, You Hua" sort="Xie, You Hua" uniqKey="Xie Y" first="You Hua" last="Xie">You Hua Xie</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Pei, Gang" sort="Pei, Gang" uniqKey="Pei G" first="Gang" last="Pei">Gang Pei</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia Rui" last="Wu">Jia Rui Wu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><affiliation wicri:level="1"><nlm:aff id="aff1">Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">E-institutes of Shanghai Universities Immunology Division, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>E-institutes of Shanghai Universities Immunology Division</wicri:regionArea></affiliation></author></analytic><series><title level="j">Febs Letters</title><idno type="ISSN">0014-5793</idno><idno type="eISSN">1873-3468</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">FEBS Lett</journal-id><journal-id journal-id-type="iso-abbrev">FEBS Lett</journal-id><journal-title-group><journal-title>Febs Letters</journal-title></journal-title-group><issn pub-type="ppub">0014-5793</issn><issn pub-type="epub">1873-3468</issn><publisher><publisher-name>Federation of European Biochemical Societies. Published by Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15811330</article-id><article-id pub-id-type="pmc">7094314</article-id><article-id pub-id-type="publisher-id">S0014-5793(05)00308-X</article-id><article-id pub-id-type="doi">10.1016/j.febslet.2005.02.070</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Wei</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Xiao-Dong</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Shi</surname><given-names>Mu De</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Rui Fu</given-names></name><xref rid="aff4" ref-type="aff">d</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>He</surname><given-names>You Yu</given-names></name><xref rid="aff2" ref-type="aff">b</xref><xref rid="fn1" ref-type="fn">1</xref></contrib><contrib contrib-type="author"><name><surname>Bian</surname><given-names>Chao</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Shi</surname><given-names>Tie Liu</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Sheng</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Zhu</surname><given-names>Xue-Liang</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Wei-Hong</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Yi Xue</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Yan</surname><given-names>Lin-Chen</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Ji</surname><given-names>Yong Yong</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Ying</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Guo-Mei</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Tian</surname><given-names>Lin</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Jin</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hong Xia</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Xie</surname><given-names>You Hua</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Pei</surname><given-names>Gang</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Jia Rui</given-names></name><email>wujr@sibs.ac.cn</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Sun</surname><given-names>Bing</given-names></name><email>bsun@sibs.ac.cn</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff5" ref-type="aff">e</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</aff><aff id="aff3"><label>b</label>Bioinformation Center, Shanghai 200031, China</aff><aff id="aff4"><label>c</label>Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China</aff><aff id="aff2"><label>d</label>Institute of Microbiology and Epidemiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100071, China</aff><aff id="aff5"><label>e</label>E-institutes of Shanghai Universities Immunology Division, China</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding authors. Fax: +86 21 54921011 <email>wujr@sibs.ac.cn</email><email>bsun@sibs.ac.cn</email></corresp><fn id="fn1"><label>1</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="pmc-release"><day>13</day><month>3</month><year>2005</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>11</day><month>4</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>13</day><month>3</month><year>2005</year></pub-date><volume>579</volume><issue>10</issue><fpage>2130</fpage><lpage>2136</lpage><history><date date-type="received"><day>13</day><month>1</month><year>2005</year></date><date date-type="rev-recd"><day>12</day><month>2</month><year>2005</year></date><date date-type="accepted"><day>25</day><month>2</month><year>2005</year></date></history><permissions><copyright-statement>Copyright © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2005</copyright-year><copyright-holder>Federation of European Biochemical Societies</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.</p></abstract><kwd-group><title>Abbreviations</title><kwd>SARS-CoV, severe acute respiratory syndrome-associated coronavirus</kwd><kwd>Abs, antibodies</kwd></kwd-group><kwd-group><title>Keywords</title><kwd>Severe acute respiratory syndrome coronavirus</kwd><kwd>Spike protein</kwd><kwd>Synthetic peptide</kwd><kwd>Syncytia formation and disease prevention</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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