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Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Identifieur interne : 000654 ( Pmc/Curation ); précédent : 000653; suivant : 000655

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Auteurs : Bin Wang ; Simon J. Potter ; Yiguang Lin ; Anthony L. Cunningham ; Dominic E. Dwyer ; Yuelong Su ; Xuejun Ma ; Yunde Hou ; Nitin K. Saksena

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2005.

RBID : PMC:1153787

Abstract

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153787

DOI: 10.1128/JCM.43.5.2339-2344.2005
PubMed: 15872263
PubMed Central: 1153787

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PMC:1153787

Le document en format XML

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<author><name sortKey="Hou, Yunde" sort="Hou, Yunde" uniqKey="Hou Y" first="Yunde" last="Hou">Yunde Hou</name>
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<series><title level="j">Journal of Clinical Microbiology</title>
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<front><div type="abstract" xml:lang="en"><p>The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (<italic>n</italic>
 = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</p>
</div>
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  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">jcm</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
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<article-meta><article-id pub-id-type="pmid">15872263</article-id>
<article-id pub-id-type="pmc">1153787</article-id>
<article-id pub-id-type="publisher-id">1985-04</article-id>
<article-id pub-id-type="doi">10.1128/JCM.43.5.2339-2344.2005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Virology</subject>
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</article-categories>
<title-group><article-title>Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Bin</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Potter</surname>
<given-names>Simon J.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lin</surname>
<given-names>Yiguang</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cunningham</surname>
<given-names>Anthony L.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Dwyer</surname>
<given-names>Dominic E.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Su</surname>
<given-names>Yuelong</given-names>
</name>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ma</surname>
<given-names>Xuejun</given-names>
</name>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hou</surname>
<given-names>Yunde</given-names>
</name>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Saksena</surname>
<given-names>Nitin K.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
</contrib-group>
<aff id="aff1">Centre for Virus Research, Westmead Millennium Institute, The University of Sydney,<label>1</label>
 Department of Health Sciences, University of Technology,<label>2</label>
 CIDMLS, ICPMR, Westmead Hospital, Sydney, New South Wales, Australia,<label>3</label>
 Beijing JDK Bio-technology Institute, National Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China<label>4</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address: Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Darcy Rd., Westmead, Sydney, NSW 2145, Australia. Phone: 61-2-98459107. Fax: 61-2-98459103. E-mail: <email>bin_wang@wmi.usyd.edu.au</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>5</month>
<year>2005</year>
</pub-date>
<volume>43</volume>
<issue>5</issue>
<fpage>2339</fpage>
<lpage>2344</lpage>
<history><date date-type="received"><day>28</day>
<month>10</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>17</day>
<month>11</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>5</day>
<month>1</month>
<year>2005</year>
</date>
</history>
<copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement>
<copyright-year>2005</copyright-year>
<abstract><p>The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (<italic>n</italic>
 = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</p>
</abstract>
</article-meta>
</front>
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</record>

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Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

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Abstract

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