Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer
Identifieur interne : 000451 ( Pmc/Curation ); précédent : 000450; suivant : 000452Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer
Auteurs : Alexandra C. Walls ; M. Alejandra Tortorici ; Berend-Jan Bosch [Pays-Bas] ; Brandon Frenz ; Peter J. M. Rottier [Pays-Bas] ; Frank Dimaio ; Félix A. Rey ; David VeeslerSource :
- Nature [ 0028-0836 ] ; 2016.
Abstract
The high-resolution cryo-electron microscopy structure of a pre-fusion coronavirus spike trimer from mouse hepatitis virus is presented; the structure reveals architectural similarities to paramyxovirus F proteins, suggesting that these fusion proteins may have evolved from a distant common ancestor.
The online version of this article (doi:10.1038/nature16988) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/nature16988
PubMed: 26855426
PubMed Central: 5018210
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Alexandra C. Walls<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</nlm:aff><wicri:noCountry code="subfield">98195 Washington USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution>Institut Pasteur, Unité de Virologie,</institution></institution-wrap>Structurale, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4444.0</institution-id><institution-id institution-id-type="ISNI">0000 0001 2112 9282</institution-id><institution>CNRS UMR,</institution></institution-wrap>3569 Virologie, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4444.0</institution-id><institution-id institution-id-type="ISNI">0000 0001 2112 9282</institution-id><institution>CNRS UMR,</institution></institution-wrap>3569 Virologie, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</nlm:aff><wicri:noCountry code="subfield">98195 Washington USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</nlm:aff><wicri:noCountry code="subfield">98195 Washington USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution>Institut Pasteur, Unité de Virologie,</institution></institution-wrap>Structurale, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4444.0</institution-id><institution-id institution-id-type="ISNI">0000 0001 2112 9282</institution-id><institution>CNRS UMR,</institution></institution-wrap>3569 Virologie, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4444.0</institution-id><institution-id institution-id-type="ISNI">0000 0001 2112 9282</institution-id><institution>CNRS UMR,</institution></institution-wrap>3569 Virologie, 75015 Paris France</nlm:aff><wicri:noCountry code="subfield">75015 Paris France</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</nlm:aff><wicri:noCountry code="subfield">98195 Washington USA</wicri:noCountry></affiliation>Le document en format XML
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id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</nlm:aff><wicri:noCountry code="subfield">98195 Washington USA</wicri:noCountry></affiliation></author></analytic><series><title level="j">Nature</title><idno type="ISSN">0028-0836</idno><idno type="eISSN">1476-4687</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The high-resolution cryo-electron microscopy structure of a pre-fusion coronavirus spike trimer from mouse hepatitis virus is presented; the structure reveals architectural similarities to paramyxovirus F proteins, suggesting that these fusion proteins may have evolved from a distant common ancestor.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/nature16988) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mclellan, Js" uniqKey="Mclellan J">JS McLellan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin, Hs" uniqKey="Yin H">HS Yin</name></author><author><name sortKey="Wen, X" uniqKey="Wen X">X Wen</name></author><author><name sortKey="Paterson, Rg" uniqKey="Paterson R">RG Paterson</name></author><author><name sortKey="Lamb, Ra" uniqKey="Lamb R">RA Lamb</name></author><author><name sortKey="Jardetzky, Ts" uniqKey="Jardetzky T">TS 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sortKey="Vigouroux, A" uniqKey="Vigouroux A">A Vigouroux</name></author><author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name></author><author><name sortKey="Rey, Fa" uniqKey="Rey F">FA Rey</name></author><author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, J" uniqKey="Gao J">J Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Supekar, Vm" uniqKey="Supekar V">VM Supekar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M Farzan</name></author><author><name sortKey="Harrison, Sc" uniqKey="Harrison S">SC 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Nature</journal-id><journal-id journal-id-type="iso-abbrev">Nature</journal-id><journal-title-group><journal-title>Nature</journal-title></journal-title-group><issn pub-type="ppub">0028-0836</issn><issn pub-type="epub">1476-4687</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26855426</article-id><article-id pub-id-type="pmc">5018210</article-id><article-id pub-id-type="publisher-id">BFnature16988</article-id><article-id pub-id-type="doi">10.1038/nature16988</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Walls</surname><given-names>Alexandra C.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Tortorici</surname><given-names>M. Alejandra</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Bosch</surname><given-names>Berend-Jan</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Frenz</surname><given-names>Brandon</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Rottier</surname><given-names>Peter J. M.</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>DiMaio</surname><given-names>Frank</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Rey</surname><given-names>Félix A.</given-names></name><address><email>dveesler@uw.edu</email></address><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Veesler</surname><given-names>David</given-names></name><address><email>felix.rey@pasteur.fr</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, 98195 Washington USA</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution>Institut Pasteur, Unité de Virologie,</institution></institution-wrap>Structurale, 75015 Paris France</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.4444.0</institution-id><institution-id institution-id-type="ISNI">0000 0001 2112 9282</institution-id><institution>CNRS UMR,</institution></institution-wrap>3569 Virologie, 75015 Paris France</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology,</institution><institution>Faculty of Veterinary Medicine, Utrecht University,</institution></institution-wrap>3584 CL, Utrecht, The Netherlands</aff></contrib-group><pub-date pub-type="epub"><day>8</day><month>2</month><year>2016</year></pub-date><pub-date pub-type="ppub"><year>2016</year></pub-date><volume>531</volume><issue>7592</issue><fpage>114</fpage><lpage>117</lpage><history><date date-type="received"><day>17</day><month>11</month><year>2015</year></date><date date-type="accepted"><day>13</day><month>1</month><year>2016</year></date></history><permissions><copyright-statement>© Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. 2016</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1" abstract-type="web-summary"><p id="Par1">The high-resolution cryo-electron microscopy structure of a pre-fusion coronavirus spike trimer from mouse hepatitis virus is presented; the structure reveals architectural similarities to paramyxovirus F proteins, suggesting that these fusion proteins may have evolved from a distant common ancestor.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/nature16988) contains supplementary material, which is available to authorized users.</p></sec></abstract><abstract id="Abs2" abstract-type="LongSummary"><title>Coronavirus spike structure</title><p id="Par2">Coronaviruses are responsible for respiratory infections worldwide, many of them mild, but also including severe pneumonia and the recent SARS and MERS outbreaks. The entry of coronaviruses into cells is mediated by the virus glycoprotein spike trimer, which contains the receptor-binding domain, as well as membrane fusion domains. Two papers published in this issue of <italic>Nature</italic> provide high-resolution (4Å) cryo-electron microscopy structures of pre-fusion coronavirus spike trimers. David Veesler and colleagues studied the trimer from murine hepatitis virus; Andrew Ward and colleagues used the human betacoronavirus HKU1, a cause of mild respiratory disease. The structures reveal mechanistic insights into the viral fusion process and architectural similarities to paramyxovirus F proteins, suggesting that these fusion proteins may have evolved from a distant common ancestor.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/nature16988) contains supplementary material, which is available to authorized users.</p></sec></abstract><abstract id="Abs3"><p id="Par3">The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. Entry of coronaviruses into cells is mediated by the transmembrane spike glycoprotein S, which forms a trimer carrying receptor-binding and membrane fusion functions<sup><xref ref-type="bibr" rid="CR1">1</xref></sup>. S also contains the principal antigenic determinants and is the target of neutralizing antibodies. Here we present the structure of a mouse coronavirus S trimer ectodomain determined at 4.0 Å resolution by single particle cryo-electron microscopy. It reveals the metastable pre-fusion architecture of S and highlights key interactions stabilizing it. The structure shares a common core with paramyxovirus F proteins<sup><xref ref-type="bibr" rid="CR2">2</xref>,<xref ref-type="bibr" rid="CR3">3</xref></sup>, implicating mechanistic similarities and an evolutionary connection between these viral fusion proteins. The accessibility of the highly conserved fusion peptide at the periphery of the trimer indicates potential vaccinology strategies to elicit broadly neutralizing antibodies against coronaviruses. Finally, comparison with crystal structures of human coronavirus S domains allows rationalization of the molecular basis for species specificity based on the use of spatially contiguous but distinct domains.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/nature16988) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>Viral membrane fusion</kwd><kwd>Cryoelectron microscopy</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Nature Limited 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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