SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway
Identifieur interne : 000345 ( Pmc/Curation ); précédent : 000344; suivant : 000346SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway
Auteurs : Hongliang Wang [République populaire de Chine] ; Peng Yang [République populaire de Chine] ; Kangtai Liu [République populaire de Chine] ; Feng Guo [République populaire de Chine] ; Yanli Zhang [République populaire de Chine] ; Gongyi Zhang [États-Unis] ; Chengyu Jiang [République populaire de Chine]Source :
- Cell Research [ 1001-0602 ] ; 2008.
Abstract
While severe acute respiratory syndrome coronavirus (SARS-CoV) was initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that virus entry may also involve endocytosis. We have found that SARS-CoV enters cells via pH- and receptor-dependent endocytosis. Treatment of cells with either SARS-CoV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic pathway inhibitors and dominant-negative Eps15 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research.
The online version of this article (doi:10.1038/cr.2008.15) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/cr.2008.15
PubMed: 18227861
PubMed Central: 7091891
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000345
Links to Exploration step
PMC:7091891Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway</title>
<author><name sortKey="Wang, Hongliang" sort="Wang, Hongliang" uniqKey="Wang H" first="Hongliang" last="Wang">Hongliang Wang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Kangtai" sort="Liu, Kangtai" uniqKey="Liu K" first="Kangtai" last="Liu">Kangtai Liu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Gongyi" sort="Zhang, Gongyi" uniqKey="Zhang G" first="Gongyi" last="Zhang">Gongyi Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.240341.0</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 0396 0728</institution-id>
<institution>Department of Immunology,</institution>
<institution>National Jewish Medical and Research Center,</institution>
</institution-wrap>
Denver, 80206 CO USA</nlm:aff>
<country>États-Unis</country>
<placeName><region type="state">Colorado</region>
</placeName>
<wicri:regionArea>Denver</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">18227861</idno>
<idno type="pmc">7091891</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091891</idno>
<idno type="RBID">PMC:7091891</idno>
<idno type="doi">10.1038/cr.2008.15</idno>
<date when="2008">2008</date>
<idno type="wicri:Area/Pmc/Corpus">000345</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000345</idno>
<idno type="wicri:Area/Pmc/Curation">000345</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000345</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway</title>
<author><name sortKey="Wang, Hongliang" sort="Wang, Hongliang" uniqKey="Wang H" first="Hongliang" last="Wang">Hongliang Wang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Kangtai" sort="Liu, Kangtai" uniqKey="Liu K" first="Kangtai" last="Liu">Kangtai Liu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Gongyi" sort="Zhang, Gongyi" uniqKey="Zhang G" first="Gongyi" last="Zhang">Gongyi Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.240341.0</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 0396 0728</institution-id>
<institution>Department of Immunology,</institution>
<institution>National Jewish Medical and Research Center,</institution>
</institution-wrap>
Denver, 80206 CO USA</nlm:aff>
<country>États-Unis</country>
<placeName><region type="state">Colorado</region>
</placeName>
<wicri:regionArea>Denver</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Cell Research</title>
<idno type="ISSN">1001-0602</idno>
<idno type="eISSN">1748-7838</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">While severe acute respiratory syndrome coronavirus (SARS-CoV) was initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that virus entry may also involve endocytosis. We have found that SARS-CoV enters cells via pH- and receptor-dependent endocytosis. Treatment of cells with either SARS-CoV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic pathway inhibitors and dominant-negative Eps15 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research.</p>
<sec><title>Supplementary information</title>
<p>The online version of this article (doi:10.1038/cr.2008.15) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name>
</author>
<author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name>
</author>
<author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pelkmans, L" uniqKey="Pelkmans L">L Pelkmans</name>
</author>
<author><name sortKey="Helenius, A" uniqKey="Helenius A">A Helenius</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sieczkarski, Sb" uniqKey="Sieczkarski S">SB Sieczkarski</name>
</author>
<author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fackler, Ot" uniqKey="Fackler O">OT Fackler</name>
</author>
<author><name sortKey="Peterlin, Bm" uniqKey="Peterlin B">BM Peterlin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matlin, Ks" uniqKey="Matlin K">KS Matlin</name>
</author>
<author><name sortKey="Reggio, H" uniqKey="Reggio H">H Reggio</name>
</author>
<author><name sortKey="Helenius, A" uniqKey="Helenius A">A Helenius</name>
</author>
<author><name sortKey="Simons, K" uniqKey="Simons K">K Simons</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sieczkarski, Sb" uniqKey="Sieczkarski S">SB Sieczkarski</name>
</author>
<author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nunes Correia, I" uniqKey="Nunes Correia I">I Nunes-Correia</name>
</author>
<author><name sortKey="Eulalio, A" uniqKey="Eulalio A">A Eulalio</name>
</author>
<author><name sortKey="Nir, S" uniqKey="Nir S">S Nir</name>
</author>
<author><name sortKey="Pedroso De Lima, Mc" uniqKey="Pedroso De Lima M">MC Pedroso de Lima</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ng, Ml" uniqKey="Ng M">ML Ng</name>
</author>
<author><name sortKey="Tan, Sh" uniqKey="Tan S">SH Tan</name>
</author>
<author><name sortKey="See, Ee" uniqKey="See E">EE See</name>
</author>
<author><name sortKey="Ooi, Ee" uniqKey="Ooi E">EE Ooi</name>
</author>
<author><name sortKey="Ling, Ae" uniqKey="Ling A">AE Ling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Qinfen, Z" uniqKey="Qinfen Z">Z Qinfen</name>
</author>
<author><name sortKey="Jinming, C" uniqKey="Jinming C">C Jinming</name>
</author>
<author><name sortKey="Xiaojun, H" uniqKey="Xiaojun H">H Xiaojun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Simmons, G" uniqKey="Simmons G">G Simmons</name>
</author>
<author><name sortKey="Reeves, Jd" uniqKey="Reeves J">JD Reeves</name>
</author>
<author><name sortKey="Rennekamp, Aj" uniqKey="Rennekamp A">AJ Rennekamp</name>
</author>
<author><name sortKey="Amberg, Sm" uniqKey="Amberg S">SM Amberg</name>
</author>
<author><name sortKey="Piefer, Aj" uniqKey="Piefer A">AJ Piefer</name>
</author>
<author><name sortKey="Bates, P" uniqKey="Bates P">P Bates</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name>
</author>
<author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name>
</author>
<author><name sortKey="Ganesh, L" uniqKey="Ganesh L">L Ganesh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, Ic" uniqKey="Huang I">IC Huang</name>
</author>
<author><name sortKey="Bosch, Bj" uniqKey="Bosch B">BJ Bosch</name>
</author>
<author><name sortKey="Li, F" uniqKey="Li F">F Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Simmons, G" uniqKey="Simmons G">G Simmons</name>
</author>
<author><name sortKey="Gosalia, Dn" uniqKey="Gosalia D">DN Gosalia</name>
</author>
<author><name sortKey="Rennekamp, Aj" uniqKey="Rennekamp A">AJ Rennekamp</name>
</author>
<author><name sortKey="Reeves, Jd" uniqKey="Reeves J">JD Reeves</name>
</author>
<author><name sortKey="Diamond, Sl" uniqKey="Diamond S">SL Diamond</name>
</author>
<author><name sortKey="Bates, P" uniqKey="Bates P">P Bates</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moore, Mj" uniqKey="Moore M">MJ Moore</name>
</author>
<author><name sortKey="Dorfman, T" uniqKey="Dorfman T">T Dorfman</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuba, K" uniqKey="Kuba K">K Kuba</name>
</author>
<author><name sortKey="Imai, Y" uniqKey="Imai Y">Y Imai</name>
</author>
<author><name sortKey="Rao, S" uniqKey="Rao S">S Rao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ray, Rb" uniqKey="Ray R">RB Ray</name>
</author>
<author><name sortKey="Basu, A" uniqKey="Basu A">A Basu</name>
</author>
<author><name sortKey="Steele, R" uniqKey="Steele R">R Steele</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blanchard, E" uniqKey="Blanchard E">E Blanchard</name>
</author>
<author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S Belouzard</name>
</author>
<author><name sortKey="Goueslain, L" uniqKey="Goueslain L">L Goueslain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Codran, A" uniqKey="Codran A">A Codran</name>
</author>
<author><name sortKey="Royer, C" uniqKey="Royer C">C Royer</name>
</author>
<author><name sortKey="Jaeck, D" uniqKey="Jaeck D">D Jaeck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Empig, Cj" uniqKey="Empig C">CJ Empig</name>
</author>
<author><name sortKey="Goldsmith, Ma" uniqKey="Goldsmith M">MA Goldsmith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dautry Varsat, A" uniqKey="Dautry Varsat A">A Dautry-Varsat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dautry Varsat, A" uniqKey="Dautry Varsat A">A Dautry-Varsat</name>
</author>
<author><name sortKey="Ciechanover, A" uniqKey="Ciechanover A">A Ciechanover</name>
</author>
<author><name sortKey="Lodish, Hf" uniqKey="Lodish H">HF Lodish</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Harding, C" uniqKey="Harding C">C Harding</name>
</author>
<author><name sortKey="Heuser, J" uniqKey="Heuser J">J Heuser</name>
</author>
<author><name sortKey="Stahl, P" uniqKey="Stahl P">P Stahl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Lh" uniqKey="Wang L">LH Wang</name>
</author>
<author><name sortKey="Rothberg, Kg" uniqKey="Rothberg K">KG Rothberg</name>
</author>
<author><name sortKey="Anderson, Rg" uniqKey="Anderson R">RG Anderson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benmerah, A" uniqKey="Benmerah A">A Benmerah</name>
</author>
<author><name sortKey="Bayrou, M" uniqKey="Bayrou M">M Bayrou</name>
</author>
<author><name sortKey="Cerf Bensussan, N" uniqKey="Cerf Bensussan N">N Cerf-Bensussan</name>
</author>
<author><name sortKey="Dautry Varsat, A" uniqKey="Dautry Varsat A">A Dautry-Varsat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benmerah, A" uniqKey="Benmerah A">A Benmerah</name>
</author>
<author><name sortKey="Poupon, V" uniqKey="Poupon V">V Poupon</name>
</author>
<author><name sortKey="Cerf Bensussan, N" uniqKey="Cerf Bensussan N">N Cerf-Bensussan</name>
</author>
<author><name sortKey="Dautry Varsat, A" uniqKey="Dautry Varsat A">A Dautry-Varsat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stan, Rv" uniqKey="Stan R">RV Stan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Orlandi, Pa" uniqKey="Orlandi P">PA Orlandi</name>
</author>
<author><name sortKey="Fishman, Ph" uniqKey="Fishman P">PH Fishman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sanchez San Martin, C" uniqKey="Sanchez San Martin C">C Sanchez-San Martin</name>
</author>
<author><name sortKey="Lopez, T" uniqKey="Lopez T">T Lopez</name>
</author>
<author><name sortKey="Arias, Cf" uniqKey="Arias C">CF Arias</name>
</author>
<author><name sortKey="Lopez, S" uniqKey="Lopez S">S Lopez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lanzrein, M" uniqKey="Lanzrein M">M Lanzrein</name>
</author>
<author><name sortKey="Schlegel, A" uniqKey="Schlegel A">A Schlegel</name>
</author>
<author><name sortKey="Kempf, C" uniqKey="Kempf C">C Kempf</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cantin, C" uniqKey="Cantin C">C Cantin</name>
</author>
<author><name sortKey="Holguera, J" uniqKey="Holguera J">J Holguera</name>
</author>
<author><name sortKey="Ferreira, L" uniqKey="Ferreira L">L Ferreira</name>
</author>
<author><name sortKey="Villar, E" uniqKey="Villar E">E Villar</name>
</author>
<author><name sortKey="Munoz Barroso, I" uniqKey="Munoz Barroso I">I Munoz-Barroso</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Gm" uniqKey="Li G">GM Li</name>
</author>
<author><name sortKey="Li, Yg" uniqKey="Li Y">YG Li</name>
</author>
<author><name sortKey="Yamate, M" uniqKey="Yamate M">M Yamate</name>
</author>
<author><name sortKey="Li, Sm" uniqKey="Li S">SM Li</name>
</author>
<author><name sortKey="Ikuta, K" uniqKey="Ikuta K">K Ikuta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
<author><name sortKey="Hoever, G" uniqKey="Hoever G">G Hoever</name>
</author>
<author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mossel, Ec" uniqKey="Mossel E">EC Mossel</name>
</author>
<author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name>
</author>
<author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name>
</author>
<author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name>
</author>
<author><name sortKey="Tesh, Rb" uniqKey="Tesh R">RB Tesh</name>
</author>
<author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mirre, C" uniqKey="Mirre C">C Mirre</name>
</author>
<author><name sortKey="Monlauzeur, L" uniqKey="Monlauzeur L">L Monlauzeur</name>
</author>
<author><name sortKey="Garcia, M" uniqKey="Garcia M">M Garcia</name>
</author>
<author><name sortKey="Delgrossi, Mh" uniqKey="Delgrossi M">MH Delgrossi</name>
</author>
<author><name sortKey="Le Bivic, A" uniqKey="Le Bivic A">A Le Bivic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Inoue, Y" uniqKey="Inoue Y">Y Inoue</name>
</author>
<author><name sortKey="Tanaka, N" uniqKey="Tanaka N">N Tanaka</name>
</author>
<author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y Tanaka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hamming, I" uniqKey="Hamming I">I Hamming</name>
</author>
<author><name sortKey="Timens, W" uniqKey="Timens W">W Timens</name>
</author>
<author><name sortKey="Bulthuis, Ml" uniqKey="Bulthuis M">ML Bulthuis</name>
</author>
<author><name sortKey="Lely, At" uniqKey="Lely A">AT Lely</name>
</author>
<author><name sortKey="Navis, Gj" uniqKey="Navis G">GJ Navis</name>
</author>
<author><name sortKey="Van Goor, H" uniqKey="Van Goor H">H van Goor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Popik, W" uniqKey="Popik W">W Popik</name>
</author>
<author><name sortKey="Alce, Tm" uniqKey="Alce T">TM Alce</name>
</author>
<author><name sortKey="Au, Wc" uniqKey="Au W">WC Au</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manes, S" uniqKey="Manes S">S Manes</name>
</author>
<author><name sortKey="Del Real, G" uniqKey="Del Real G">G del Real</name>
</author>
<author><name sortKey="Martinez, Ac" uniqKey="Martinez A">AC Martinez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shigeta, S" uniqKey="Shigeta S">S Shigeta</name>
</author>
<author><name sortKey="Yamase, T" uniqKey="Yamase T">T Yamase</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kliger, Y" uniqKey="Kliger Y">Y Kliger</name>
</author>
<author><name sortKey="Levanon, Ey" uniqKey="Levanon E">EY Levanon</name>
</author>
<author><name sortKey="Gerber, D" uniqKey="Gerber D">D Gerber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Negre, D" uniqKey="Negre D">D Negre</name>
</author>
<author><name sortKey="Mangeot, Pe" uniqKey="Mangeot P">PE Mangeot</name>
</author>
<author><name sortKey="Duisit, G" uniqKey="Duisit G">G Duisit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kohler, G" uniqKey="Kohler G">G Kohler</name>
</author>
<author><name sortKey="Milstein, C" uniqKey="Milstein C">C Milstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kee, Sh" uniqKey="Kee S">SH Kee</name>
</author>
<author><name sortKey="Cho, Ej" uniqKey="Cho E">EJ Cho</name>
</author>
<author><name sortKey="Song, Jw" uniqKey="Song J">JW Song</name>
</author>
<author><name sortKey="Park, Ks" uniqKey="Park K">KS Park</name>
</author>
<author><name sortKey="Baek, Lj" uniqKey="Baek L">LJ Baek</name>
</author>
<author><name sortKey="Song, Kj" uniqKey="Song K">KJ Song</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meertens, L" uniqKey="Meertens L">L Meertens</name>
</author>
<author><name sortKey="Bertaux, C" uniqKey="Bertaux C">C Bertaux</name>
</author>
<author><name sortKey="Dragic, T" uniqKey="Dragic T">T Dragic</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cell Res</journal-id>
<journal-id journal-id-type="iso-abbrev">Cell Res</journal-id>
<journal-title-group><journal-title>Cell Research</journal-title>
</journal-title-group>
<issn pub-type="ppub">1001-0602</issn>
<issn pub-type="epub">1748-7838</issn>
<publisher><publisher-name>Nature Publishing Group UK</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18227861</article-id>
<article-id pub-id-type="pmc">7091891</article-id>
<article-id pub-id-type="publisher-id">BFcr200815</article-id>
<article-id pub-id-type="doi">10.1038/cr.2008.15</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Hongliang</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Peng</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Kangtai</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Guo</surname>
<given-names>Feng</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Yanli</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Gongyi</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Jiang</surname>
<given-names>Chengyu</given-names>
</name>
<address><phone>+86-10-65296908</phone>
<fax>+86-10-65276551</fax>
<email>jiang@pumc.edu.cn</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.12527.33</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id>
<institution>National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences,</institution>
</institution-wrap>
Beijing, 100005 China</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.240341.0</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 0396 0728</institution-id>
<institution>Department of Immunology,</institution>
<institution>National Jewish Medical and Research Center,</institution>
</institution-wrap>
Denver, 80206 CO USA</aff>
</contrib-group>
<pub-date pub-type="epub"><day>29</day>
<month>1</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><month>2</month>
<year>2008</year>
</pub-date>
<volume>18</volume>
<issue>2</issue>
<fpage>290</fpage>
<lpage>301</lpage>
<history><date date-type="received"><day>27</day>
<month>11</month>
<year>2007</year>
</date>
<date date-type="rev-recd"><day>29</day>
<month>11</month>
<year>2007</year>
</date>
<date date-type="accepted"><day>30</day>
<month>11</month>
<year>2007</year>
</date>
</history>
<permissions><copyright-statement>© Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2008</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">While severe acute respiratory syndrome coronavirus (SARS-CoV) was initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that virus entry may also involve endocytosis. We have found that SARS-CoV enters cells via pH- and receptor-dependent endocytosis. Treatment of cells with either SARS-CoV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic pathway inhibitors and dominant-negative Eps15 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research.</p>
<sec><title>Supplementary information</title>
<p>The online version of this article (doi:10.1038/cr.2008.15) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)</kwd>
<kwd>endocytosis</kwd>
<kwd>angiotensin-converting enzyme 2 (ACE2)</kwd>
<kwd>lipid rafts</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© IBCB, SIBS, CAS 2008</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000345 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000345 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:7091891 |texte= SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:18227861" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |