First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood
Identifieur interne : 000301 ( Pmc/Curation ); précédent : 000300; suivant : 000302First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood
Auteurs : Weimin Zhou ; Wen Wang ; Huijuan Wang ; Roujian Lu ; Wenjie TanSource :
- BMC Infectious Diseases [ 1471-2334 ] ; 2013.
Abstract
Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknown.
To measure antibodies to the spike (S) protein of the four common non-SARS HCoVs, recombinant S proteins of the four HCoVs were expressed and characterised in 293 T cell. An S-protein-based indirect immunofluorescence assay (IFA) was then developed to detect anti-S IgG and IgM for the four individual HCoVs and applied to serum samples from a general asymptomatic population (218 children and 576 adults) in Beijing.
Of 794 blood samples tested, only 29 (3.65%) were negative for anti-S IgG. The seropositivity of the four anti-S IgG antibodies was >70% within the general population. The majority of seroconversions to four-HCoV positivity first occurred in children. Both S-IgG and S-IgM antibodies were detectable among children and increased with age, reaching a plateau at 6 years of age. However, no anti-S IgM was detected in healthy adults.
Large proportions of children and adults in Beijing have evidence of anti-S IgG against four the HCoVs, and first infections by all four non-SARS HCoVs takes place during childhood.
The online version of this article (doi:10.1186/1471-2334-13-433) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2334-13-433
PubMed: 24040960
PubMed Central: 3848659
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood</title>
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<author><name sortKey="Lu, Roujian" sort="Lu, Roujian" uniqKey="Lu R" first="Roujian" last="Lu">Roujian Lu</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknown.</p>
</sec>
<sec><title>Methods</title>
<p>To measure antibodies to the spike (S) protein of the four common non-SARS HCoVs, recombinant S proteins of the four HCoVs were expressed and characterised in 293 T cell. An S-protein-based indirect immunofluorescence assay (IFA) was then developed to detect anti-S IgG and IgM for the four individual HCoVs and applied to serum samples from a general asymptomatic population (218 children and 576 adults) in Beijing.</p>
</sec>
<sec><title>Results</title>
<p>Of 794 blood samples tested, only 29 (3.65%) were negative for anti-S IgG. The seropositivity of the four anti-S IgG antibodies was >70% within the general population. The majority of seroconversions to four-HCoV positivity first occurred in children. Both S-IgG and S-IgM antibodies were detectable among children and increased with age, reaching a plateau at 6 years of age. However, no anti-S IgM was detected in healthy adults.</p>
</sec>
<sec><title>Conclusion</title>
<p>Large proportions of children and adults in Beijing have evidence of anti-S IgG against four the HCoVs, and first infections by all four non-SARS HCoVs takes place during childhood.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/1471-2334-13-433) contains supplementary material, which is available to authorized users.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Infect Dis</journal-id>
<journal-id journal-id-type="iso-abbrev">BMC Infect. Dis</journal-id>
<journal-title-group><journal-title>BMC Infectious Diseases</journal-title>
</journal-title-group>
<issn pub-type="epub">1471-2334</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24040960</article-id>
<article-id pub-id-type="pmc">3848659</article-id>
<article-id pub-id-type="publisher-id">2629</article-id>
<article-id pub-id-type="doi">10.1186/1471-2334-13-433</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Zhou</surname>
<given-names>Weimin</given-names>
</name>
<address><email>doglet44@sina.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Wen</given-names>
</name>
<address><email>wangwen435@sohu.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Huijuan</given-names>
</name>
<address><email>wanghuijuan439@sina.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lu</surname>
<given-names>Roujian</given-names>
</name>
<address><email>xiaolu017@sina.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Tan</surname>
<given-names>Wenjie</given-names>
</name>
<address><email>tanwj28@yahoo.cn</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.198530.6</institution-id>
<institution-id institution-id-type="ISNI">0000000088032373</institution-id>
<institution>Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention,</institution>
<institution>China Centers for Disease Control and Prevention,</institution>
</institution-wrap>
Beijing, 102206 China</aff>
</contrib-group>
<pub-date pub-type="epub"><day>16</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>16</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="collection"><year>2013</year>
</pub-date>
<volume>13</volume>
<elocation-id>433</elocation-id>
<history><date date-type="received"><day>4</day>
<month>12</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>27</day>
<month>8</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>© Zhou et al.; licensee BioMed Central Ltd. 2013</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknown.</p>
</sec>
<sec><title>Methods</title>
<p>To measure antibodies to the spike (S) protein of the four common non-SARS HCoVs, recombinant S proteins of the four HCoVs were expressed and characterised in 293 T cell. An S-protein-based indirect immunofluorescence assay (IFA) was then developed to detect anti-S IgG and IgM for the four individual HCoVs and applied to serum samples from a general asymptomatic population (218 children and 576 adults) in Beijing.</p>
</sec>
<sec><title>Results</title>
<p>Of 794 blood samples tested, only 29 (3.65%) were negative for anti-S IgG. The seropositivity of the four anti-S IgG antibodies was >70% within the general population. The majority of seroconversions to four-HCoV positivity first occurred in children. Both S-IgG and S-IgM antibodies were detectable among children and increased with age, reaching a plateau at 6 years of age. However, no anti-S IgM was detected in healthy adults.</p>
</sec>
<sec><title>Conclusion</title>
<p>Large proportions of children and adults in Beijing have evidence of anti-S IgG against four the HCoVs, and first infections by all four non-SARS HCoVs takes place during childhood.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/1471-2334-13-433) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Human coronavirus</kwd>
<kwd>Spike protein</kwd>
<kwd>Indirect immunofluorescence assay</kwd>
<kwd>Antibody</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2013</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
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