Accessory proteins of SARS-CoV and other coronaviruses
Identifieur interne : 001690 ( Pmc/Corpus ); précédent : 001689; suivant : 001691Accessory proteins of SARS-CoV and other coronaviruses
Auteurs : Ding Xiang Liu ; To Sing Fung ; Kelvin Kian-Long Chong ; Aditi Shukla ; Rolf HilgenfeldSource :
- Antiviral Research [ 0166-3542 ] ; 2014.
Abstract
The SARS-CoV genome encodes eight accessory proteins; none is essential for RNA replication. Several appear to be involved in virus–host interactions and to influence the pathogenicity of the virus. Some of these accessory proteins modulate the interferon pathway and the production of pro-inflammatory cytokines. Five of the accessory proteins are incorporated into virions as minor structural proteins. Other coronaviruses including MERS-CoV also encode accessory proteins.
Url:
DOI: 10.1016/j.antiviral.2014.06.013
PubMed: 24995382
PubMed Central: 7113789
Links to Exploration step
PMC:7113789Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Accessory proteins of SARS-CoV and other coronaviruses</title><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Fung, To Sing" sort="Fung, To Sing" uniqKey="Fung T" first="To Sing" last="Fung">To Sing Fung</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Chong, Kelvin Kian Long" sort="Chong, Kelvin Kian Long" uniqKey="Chong K" first="Kelvin Kian-Long" last="Chong">Kelvin Kian-Long Chong</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Shukla, Aditi" sort="Shukla, Aditi" uniqKey="Shukla A" first="Aditi" last="Shukla">Aditi Shukla</name><affiliation><nlm:aff id="af010">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="af015">German Center for Infection Research (DZIF), University of Lübeck, Germany</nlm:aff></affiliation></author><author><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><affiliation><nlm:aff id="af010">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="af015">German Center for Infection Research (DZIF), University of Lübeck, Germany</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24995382</idno><idno type="pmc">7113789</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113789</idno><idno type="RBID">PMC:7113789</idno><idno type="doi">10.1016/j.antiviral.2014.06.013</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">001690</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001690</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Accessory proteins of SARS-CoV and other coronaviruses</title><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Fung, To Sing" sort="Fung, To Sing" uniqKey="Fung T" first="To Sing" last="Fung">To Sing Fung</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Chong, Kelvin Kian Long" sort="Chong, Kelvin Kian Long" uniqKey="Chong K" first="Kelvin Kian-Long" last="Chong">Kelvin Kian-Long Chong</name><affiliation><nlm:aff id="af005">School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</nlm:aff></affiliation></author><author><name sortKey="Shukla, Aditi" sort="Shukla, Aditi" uniqKey="Shukla A" first="Aditi" last="Shukla">Aditi Shukla</name><affiliation><nlm:aff id="af010">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="af015">German Center for Infection Research (DZIF), University of Lübeck, Germany</nlm:aff></affiliation></author><author><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><affiliation><nlm:aff id="af010">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="af015">German Center for Infection Research (DZIF), University of Lübeck, Germany</nlm:aff></affiliation></author></analytic><series><title level="j">Antiviral Research</title><idno type="ISSN">0166-3542</idno><idno type="eISSN">1872-9096</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0255">The SARS-CoV genome encodes eight accessory proteins; none is essential for RNA replication.</p></list-item><list-item id="o0010"><label>•</label><p id="p0260">Several appear to be involved in virus–host interactions and to influence the pathogenicity of the virus.</p></list-item><list-item id="o0015"><label>•</label><p id="p0265">Some of these accessory proteins modulate the interferon pathway and the production of pro-inflammatory cytokines.</p></list-item><list-item id="o0020"><label>•</label><p id="p0270">Five of the accessory proteins are incorporated into virions as minor structural proteins.</p></list-item><list-item id="o0025"><label>•</label><p id="p0275">Other coronaviruses including MERS-CoV also encode accessory proteins.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey=" Kerstrom, S" uniqKey=" Kerstrom S">S. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Antiviral Res</journal-id><journal-id journal-id-type="iso-abbrev">Antiviral Res</journal-id><journal-title-group><journal-title>Antiviral Research</journal-title></journal-title-group><issn pub-type="ppub">0166-3542</issn><issn pub-type="epub">1872-9096</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24995382</article-id><article-id pub-id-type="pmc">7113789</article-id><article-id pub-id-type="publisher-id">S0166-3542(14)00175-2</article-id><article-id pub-id-type="doi">10.1016/j.antiviral.2014.06.013</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Accessory proteins of SARS-CoV and other coronaviruses</article-title></title-group><contrib-group><contrib contrib-type="author" id="au005"><name><surname>Liu</surname><given-names>Ding Xiang</given-names></name><email>dxliu@ntu.edu.sg</email><xref rid="af005" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="au010"><name><surname>Fung</surname><given-names>To Sing</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au015"><name><surname>Chong</surname><given-names>Kelvin Kian-Long</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au020"><name><surname>Shukla</surname><given-names>Aditi</given-names></name><xref rid="af010" ref-type="aff">b</xref><xref rid="af015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="au025"><name><surname>Hilgenfeld</surname><given-names>Rolf</given-names></name><xref rid="af010" ref-type="aff">b</xref><xref rid="af015" ref-type="aff">c</xref></contrib></contrib-group><aff id="af005"><label>a</label>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore</aff><aff id="af010"><label>b</label>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</aff><aff id="af015"><label>c</label>German Center for Infection Research (DZIF), University of Lübeck, Germany</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author. Tel.: +65 63162862; fax: +65 67936828. <email>dxliu@ntu.edu.sg</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>1</day><month>7</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>1</day><month>7</month><year>2014</year></pub-date><volume>109</volume><fpage>97</fpage><lpage>109</lpage><history><date date-type="received"><day>13</day><month>2</month><year>2014</year></date><date date-type="rev-recd"><day>17</day><month>6</month><year>2014</year></date><date date-type="accepted"><day>23</day><month>6</month><year>2014</year></date></history><permissions><copyright-statement>Copyright © 2014 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="ab005"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0255">The SARS-CoV genome encodes eight accessory proteins; none is essential for RNA replication.</p></list-item><list-item id="o0010"><label>•</label><p id="p0260">Several appear to be involved in virus–host interactions and to influence the pathogenicity of the virus.</p></list-item><list-item id="o0015"><label>•</label><p id="p0265">Some of these accessory proteins modulate the interferon pathway and the production of pro-inflammatory cytokines.</p></list-item><list-item id="o0020"><label>•</label><p id="p0270">Five of the accessory proteins are incorporated into virions as minor structural proteins.</p></list-item><list-item id="o0025"><label>•</label><p id="p0275">Other coronaviruses including MERS-CoV also encode accessory proteins.</p></list-item></list></p></abstract><abstract id="ab010"><p>The huge RNA genome of SARS coronavirus comprises a number of open reading frames that code for a total of eight accessory proteins. Although none of these are essential for virus replication, some appear to have a role in virus pathogenesis. Notably, some SARS-CoV accessory proteins have been shown to modulate the interferon signaling pathways and the production of pro-inflammatory cytokines. The structural information on these proteins is also limited, with only two (p7a and p9b) having their structures determined by X-ray crystallography. This review makes an attempt to summarize the published knowledge on SARS-CoV accessory proteins, with an emphasis on their involvement in virus–host interaction. The accessory proteins of other coronaviruses are also briefly discussed. This paper forms part of a series of invited articles in <italic>Antiviral Research</italic> on “From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses” (see Introduction by <xref rid="b0795" ref-type="bibr">Hilgenfeld and Peiris (2013)</xref>).</p></abstract><kwd-group id="kg005"><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Other coronaviruses</kwd><kwd>Accessory proteins</kwd><kwd>Structure and function</kwd></kwd-group></article-meta></front><body><sec id="s0005"><label>1</label><title>Introduction</title><p id="p0005">The severe acute respiratory syndrome coronavirus (SARS-CoV) was identified as the etiological agent of severe acute respiratory syndrome during the 2002–2003 outbreak (<xref rid="b0005" ref-type="bibr">Peiris et al., 2003</xref>). SARS-CoV belongs to the genus <italic>Betacoronavirus</italic> of the family <italic>Coronaviridae</italic> (<xref rid="b0010" ref-type="bibr">King et al., 2011</xref>). Coronaviruses are enveloped viruses with positive-sense, non-segmented, single-stranded RNA genomes, characterized by club-like projections on the virus particles (<xref rid="b0015" ref-type="bibr">Masters, 2006</xref>). The first two thirds of the coronavirus genome encode the replicase genes, which are translated into two large polyproteins, pp1a and pp1ab, that are processed into 15 or 16 non-structural proteins (nsp) via proteolytic cleavage (<xref rid="b0020" ref-type="bibr">Thiel et al., 2003</xref>). The remaining one third of the genome contains ORFs for the structural proteins, namely the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins.</p><p id="p0010">In addition to these genomic elements shared by other coronaviruses, the SARS-CoV genome also contains eight ORFs coding for accessory proteins, namely ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b (<xref rid="f0005" ref-type="fig">Fig. 1</xref>). These proteins are specific for SARS-CoV and do not show significant homology to accessory proteins of other coronaviruses, with the exception of the SARS-like coronavirus SL-CoV-WIV1 that was recently discovered in bats (<xref rid="b0790" ref-type="bibr">Ge et al., 2013</xref>) and has the same set of accessory proteins as SARS-CoV. The potential connection between these accessory proteins and the high virulence of SARS-CoV has led to detailed structural and functional studies.<fig id="f0005"><label>Fig. 1</label><caption><p>Schematic diagram showing the genome organization of the severe acute respiratory syndrome coronavirus (SARS-CoV). The eight accessory proteins (3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b) are shown as colored boxes. The 5′ leader sequence (black box), open reading frames (ORFs 1a, 1b) encoding components of the replication/transcription complex, the structural genes spike (S), membrane (M), envelope (E), and nucleo-capsid (N) are also indicated (not drawn to scale).</p></caption><graphic xlink:href="gr1"></graphic></fig></p><p id="p0015">Coronavirus accessory proteins have been generally considered to be dispensable for viral replication <italic>in vitro</italic> (<xref rid="b0030" ref-type="bibr">de Haan et al., 2002</xref>, <xref rid="b0035" ref-type="bibr">Haijema et al., 2004</xref>, <xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>, <xref rid="b0045" ref-type="bibr">Ontiveros et al., 2001</xref>, <xref rid="b0050" ref-type="bibr">Shen et al., 2003</xref>, <xref rid="b0055" ref-type="bibr">Hodgson et al., 2006</xref>, <xref rid="b0060" ref-type="bibr">Casais et al., 2005</xref>). However, several accessory proteins have been shown to exhibit functions in virus-host interactions during coronavirus infection <italic>in vivo</italic>. For example, deletion of ORF2a (NS2), HE, ORF4, and ORF5a in the mouse hepatitis virus (MHV) led to a significant attenuation of the virus in its natural host (<xref rid="b0030" ref-type="bibr">de Haan et al., 2002</xref>). On the other hand, continuous passage of infectious bronchitis virus (IBV) in cell culture results in a mutation in the 3b gene coding for a C-terminally truncated 3b protein. Interestingly, compared with wild-type virus, the mutant virus has a growth advantage and increases virulence in the chicken embryo (<xref rid="b0050" ref-type="bibr">Shen et al., 2003</xref>). Similarly, recent studies have suggested that SARS-CoV accessory proteins may confer biological advantages to the virus in the natural host, and contribute to the pathogenesis of SARS (<xref rid="b0065" ref-type="bibr">Narayanan et al., 2008</xref>). Extensive functional studies in cell culture and animal models have shown that SARS-CoV accessory proteins are involved in a wide variety of cellular processes, such as cell proliferation, programmed cell death, activation of stress response pathways and cytokine production, to name just a few. Notably, some coronaviral accessory proteins have also been shown to modulate the interferon signaling, which is of paramount importance for host antiviral immunity (<xref rid="b0070" ref-type="bibr">Frieman et al., 2007</xref>, <xref rid="b0075" ref-type="bibr">Dedeurwaerder et al., 2014</xref>, <xref rid="b0080" ref-type="bibr">Zhang et al., 2013</xref>, <xref rid="b0085" ref-type="bibr">Cruz et al., 2013</xref>).</p><p id="p0020">As part of a series of invited articles in <italic><italic>Antiviral Research</italic></italic> on “From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses” (<xref rid="b0795" ref-type="bibr">Hilgenfeld and Peiris, 2013</xref>), this review summerizes our current knowledge on SARS-CoV accessory proteins, including an update on structural and functional studies, with a particular emphasis on the involvement of accessory proteins in virus-host interactions. In addition, we will briefly summarize the current knowledge on accessory proteins of other coronaviruses.</p></sec><sec id="s0010"><label>2</label><title>Accessory proteins of the SARS-CoV</title><sec id="s0015"><label>2.1</label><title>Orf3a</title><p id="p0560">Proteins 3a and 3b (p3a and p3b, previously also known as X1 and X2), comprise 274 and 154 amino-acid residues, respectively (<xref rid="b0090" ref-type="bibr">Marra et al., 2003</xref>, <xref rid="b0095" ref-type="bibr">Rota et al., 2003</xref>). They are encoded by ORF3a (commonly known as ORF3 or U274) and ORF3b and both make up the second largest sub-genomic RNA in the SARS-CoV genome (<xref rid="b0100" ref-type="bibr">Tan et al., 2006</xref>). So far, the structures of the p3a and p3b are still not known (<xref rid="b0105" ref-type="bibr">Bartlam et al., 2005</xref>) (see <xref rid="t0005" ref-type="table">Table 1</xref>).<table-wrap position="float" id="t0005"><label>Table 1</label><caption><p>SARS-CoV ORF3a.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p3a</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0010"><list-item id="o0030"><label>•</label><p id="p0285">Subcellular localization: punctate pattern in the cytoplasm, Golgi, plasma membrane and intracellularly</p></list-item><list-item id="o0035"><label>•</label><p id="p0290">Minor structural protein</p></list-item><list-item id="o0040"><label>•</label><p id="p0295">Not required for SARS-CoV replication</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0015"><list-item id="o0045"><label>■</label><p id="p0300">C-terminus (aa 209–264) induces cell-cycle arrest</p></list-item><list-item id="o0050"><label>■</label><p id="p0305">The YxxΦ motif (aa 160–163): internalizing proteins from the plasma membrane into endosomes; also functions in immune evasion</p></list-item><list-item id="o0055"><label>■</label><p id="p0310">The diacidic motif (aa 171–173): ER export</p></list-item><list-item id="o0060"><label>■</label><p id="p0315">Full length p3a: interacts with caveolin-1 during virus uptake and release</p></list-item><list-item id="o0065"><label>■</label><p id="p0320">Full length p3a: activates PERK pathway in the UPR and triggers apoptosis</p></list-item><list-item id="o0070"><label>■</label><p id="p0325">Full length p3a: activates p38 kinase to induce cytochrome-c mediated apoptosis</p></list-item><list-item id="o0075"><label>■</label><p id="p0330">Full length p3a: activates NF-κB and JNK, induces RANTES and IL-8 production</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0030">Protein 3a was detected <italic>in vivo</italic> or <italic>in vitro</italic> when anti-p3a antibodies were used in SARS-infected specimens or patients (<xref rid="b0110" ref-type="bibr">Yu et al., 2004</xref>). Hence, the protein can be used as a diagnostic marker for patients infected with SARS-CoV. <italic>In vivo</italic>, the presence of p3a was found in autopsy sections from lung and intestinal tissues of SARS patients (<xref rid="b0115" ref-type="bibr">Law et al., 2005</xref>). Uniquely, frameshift mutations do not necessarily cause protein 3a to be non-functional (<xref rid="b0120" ref-type="bibr">Tan et al., 2005</xref>) and even the virus can deliberately make use of frameshift mutations to code for 3a variants by exploiting hepta- and octa-uridine sites (<xref rid="b0125" ref-type="bibr">Wang et al., 2006</xref>).</p><p id="p0035">It has been shown that SARS-CoV protein 3a is a structural component by using electron microscopy (<xref rid="b0130" ref-type="bibr">Ito et al., 2005</xref>). Shen et al. supported this finding by demonstrating that the protein can be incorporated into virus-like particles (VLPs) using recombinant baculoviruses expressing E, M, and p3a (<xref rid="b0135" ref-type="bibr">Shen et al., 2005</xref>). However, for the formation of these VLPs, p3a of SARS-CoV is dispensible (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>). Being efficiently expressed on the cell surface, the protein was easily detected in a majority of SARS patients due to the triggering of a humoral and cellular immune response in these patients (<xref rid="b0140" ref-type="bibr">Lu et al., 2009</xref>). In light of immunity, p3a can cause the activation of NF-κB and JNK leading to the upregulation of RANTES and IL-8 in A549 and HEK293T cells (<xref rid="b0145" ref-type="bibr">Kanzawa et al., 2006</xref>). It has also been demonstrated to up-regulate fibrinogen mRNA and protein levels in A549 cells (<xref rid="b0150" ref-type="bibr">Tan et al., 2005</xref>).</p><p id="p0040">Protein 3a has been shown to be present in both the plasma membrane as well as intracellularly (<xref rid="b0130" ref-type="bibr">Ito et al., 2005</xref>, <xref rid="b0155" ref-type="bibr">Oostra et al., 2006</xref>, <xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>, <xref rid="b0165" ref-type="bibr">Yuan et al., 2005</xref>). It can also be detected by Western blot in SARS-CoV particles purified by sucrose gradient centrifugation. Yu et al. used confocal microscopy and fractionation to show that p3a is distributed in a “punctate pattern” in the cytoplasm while the majority of its expression is concentrated at the Golgi apparatus (<xref rid="b0110" ref-type="bibr">Yu et al., 2004</xref>). On top of all these findings, Tan et al. have shown that this protein can also be transported to the cell surface and enter the cell through endocytosis (<xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>). Additionally, through immunoprecipitation, this protein was shown to interact with SARS-CoV M, S, E, and 7a proteins (<xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>, <xref rid="b0170" ref-type="bibr">von Brunn et al., 2007</xref>).</p><p id="p0045">Being the largest accessory protein (<xref rid="b0065" ref-type="bibr">Narayanan et al., 2008</xref>), p3a has an extracellular N-terminus and an intracellular C-terminus (<xref rid="f0010" ref-type="fig">Fig. 2</xref>) <xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>, <xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>. Another feature of the protein is the presence of a cysteine-rich domain (aa 81–160) <xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>.<fig id="f0010"><label>Fig. 2</label><caption><p>Schematic diagram showing motifs and domains within SARS-CoV accessory proteins p3a and p6. TM, transmembrane domain.</p></caption><graphic xlink:href="gr2"></graphic></fig></p><p id="p0050">The N-terminal region consists of three transmembrane domains (TMDs) (<xref rid="b0180" ref-type="bibr">Tan et al., 2004</xref>) (<xref rid="f0015" ref-type="fig">Fig. 3</xref>). Recently, through computational modeling, Kruger and Fischer proposed a theoretical model of the protein being an ion channel through the use of its transmembrane domains (<xref rid="b0185" ref-type="bibr">Kruger and Fischer, 2009</xref>). Chien et al. expressed the full-length p3a and reconstituted it into lipid bilayers to characterize the ion-channel activity and suggested that either TMD 2 or 3 lines the pore of the channel (<xref rid="b0780" ref-type="bibr">Chien et al., 2013</xref>). The central region comprising residues 125–200 of p3a has been shown to be bound to the 5’ UTR of the SARS-CoV genome (<xref rid="b0195" ref-type="bibr">Sharma et al., 2007</xref>), characterized by Sharma et al. by using yeast-three-hybrid screen, electrophoretic mobility shift assay, and ultraviolet crosslinking (<xref rid="b0195" ref-type="bibr">Sharma et al., 2007</xref>). Intriguingly, just the N-terminal portion of p3a is able to evoke a strong humoral immune response (<xref rid="b0200" ref-type="bibr">Zhong et al., 2006</xref>).<fig id="f0015"><label>Fig. 3</label><caption><p>Schematic diagram showing the known topological and structural features of SARS-CoV accessory proteins. N-terminus (N) and C-terminus (C) are indicated. α-Helices are represented by blue columns and β-strands are represented by red arrows. The topological or structural features of p3b, p8a, and p8b are not well understood. Ribbon diagrams for p7a and p9b are adapted from <xref rid="b0440" ref-type="bibr">Nelson et al. (2005)</xref> and <xref rid="b0590" ref-type="bibr">Meier et al., 2006</xref>, respectively.</p></caption><graphic xlink:href="gr3"></graphic></fig></p><p id="p0055">The cysteine-rich domain of p3a serves a multitude of functions and some have suggested its role in the p3a ion channel. The p3a is able to interact with the SARS-CoV S protein by formation of disulfide linkages through its cysteine-rich regions, mainly the CWLCWKC region (aa 127–133) (<xref rid="b0205" ref-type="bibr">Zeng et al., 2004</xref>). Another important feature is the oligomerization of p3a as it is able to form homodimers and homotetramers (<xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>). Through inducing point mutations, Lu et al. showed that Cys133 is important for oligomerization of the protein and confirmed the formation of dimers and tetramers through FRET (<xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>). Utilizing <italic>Xenopus</italic> oocytes, their results were suggestive of the tetramers being able to form a potassium-permeable ion channel and modulating release of SARS-CoV particles (<xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>, <xref rid="b0210" ref-type="bibr">Shi et al., 2006</xref>). Chan et al. further validated that blocking these ion channels suppressed SARS-CoV-mediated apoptosis (<xref rid="b0215" ref-type="bibr">Chan et al., 2009</xref>).</p><p id="p0060">The C-terminal domain is hydrophilic in nature and contains both the YxxΦ (where x represents any aa and Φ is an amino acid with a hydrophobic, bulky side-chain) and the diacidic motifs (ExD, Asp-x-Glu, where x represents any aa). This domain can play a role in G1 cell-cycle arrest by depletion of cyclin D3 (<xref rid="b0220" ref-type="bibr">Yuan et al., 2007</xref>). Interestingly, p3a seems to share a similar topology with the M protein (<xref rid="b0090" ref-type="bibr">Marra et al., 2003</xref>). The YxxΦ motif plays a role in internalization of proteins into various intracellular components, including proteins from the plasma membrane into the endosomes (<xref rid="b0225" ref-type="bibr">Bonifacino and Traub, 2003</xref>, <xref rid="b0230" ref-type="bibr">Trowbridge et al., 1993</xref>). The diacidic motif is required for ER export (<xref rid="b0235" ref-type="bibr">Nishimura and Balch, 1997</xref>). Tan et al. demonstrated that deletion of the C-terminal domain would prevent the protein from being expressed on the cell surface (<xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>). Furthermore, they also hypothesized that the YxxΦ motif presented in p3a would allow the internalization of the S protein and prevent its presentation on the cell surface (<xref rid="b0240" ref-type="bibr">Tan, 2005</xref>). This could be involved in evading host immunity systems or even facilitate the assembly of SARS-CoV.</p><p id="p0565">p3a can be detected in the supernatant in two different forms from infected Vero E6 cells or Caco2 cells (<xref rid="b0175" ref-type="bibr">Lu et al., 2006</xref>, <xref rid="b0245" ref-type="bibr">Huang et al., 2006</xref>). Huang et al. demonstrated by sucrose-gradient centrifugation and densitometric scans the presence of a 37-kDa form (protein 3a-1) and a 31-kDa form (protein 3a-2). The 37-kDa form was detected in fractions with similar densities (1.18–1.20 g/ml) to the SARS-CoV particles, as opposed to fractions containing the 31-kDa form (1.13–1.15 g/ml) <xref rid="b0150" ref-type="bibr">Tan et al., 2005</xref>, <xref rid="b0220" ref-type="bibr">Yuan et al., 2007</xref>, suggesting that the 31-kDa form may be present as extracellular membrane structures while the 37-kDa form may be assembled into the mature virons. The presence of these two forms is due to the protein 3a being able to be modified post-translationally. This protein can be O-glycosylated as demonstrated by Oostra et al. through electrophoretic mobility-shift assays, transforming the unmodified 31-kDa form into a 33-kDa form (<xref rid="b0155" ref-type="bibr">Oostra et al., 2006</xref>). p3a resembles the M protein of other coronaviruses, such as MHV, in terms of (1) the subcellular localization pattern of M protein (<xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>, <xref rid="b0250" ref-type="bibr">Klumperman et al., 1994</xref>, <xref rid="b0255" ref-type="bibr">Yuan et al., 2005</xref>), (2) having an extracellular N-terminus and a C-terminal endodomain, (3) spanning the membrane three times (<xref rid="b0260" ref-type="bibr">Rottier et al., 1984</xref>, <xref rid="b0265" ref-type="bibr">Rottier et al., 1986</xref>, <xref rid="b0270" ref-type="bibr">Braakman and Van Anken, 2000</xref>), and (4) being considered a structural protein (<xref rid="b0130" ref-type="bibr">Ito et al., 2005</xref>, <xref rid="b0135" ref-type="bibr">Shen et al., 2005</xref>). Despite both M and 3a proteins being glycosylated, p3a in Ost-7 cells is neuraminidase-sensitive but not O-glycosidase sensitive, in contrast to the M protein where it is sensitive to both (<xref rid="b0155" ref-type="bibr">Oostra et al., 2006</xref>).</p><p id="p0065">Recombinant viruses with ORF3a deleted (i.e., SARS-CoV Δ3a) showed significant reduction in virus titres after infecting Vero, MA104, and Caco2 cells (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>). Interestingly, Yount et al. demonstrated that deletion of ORF3 and ORF6 decreased the overall virus titres as compared to just deleting ORF 3a alone, suggesting that ORF3a is non-essential (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>). In contrast, there is conflicting evidence that ORF3a may be essential, because of the reduction of viral titres in SARS-CoV-infected cells transfected with a siRNA targeting p3a (<xref rid="b0275" ref-type="bibr">Åkerström et al., 2007</xref>). We propose that this may be due to the subsequent knockdown of p3b as well, resulting in the lower virus production.</p><p id="p0070">Using transmission electron microscopy (TEM), Freundt et al. observed in Vero cells that only in the presence of p3a, intracellular vesicles will be formed, as observed in the pathology in SARS patients (<xref rid="b0280" ref-type="bibr">Freundt et al., 2010</xref>). The presence of Lamp-1 through immunofluorescence can also be detected in these vesicles (<xref rid="b0280" ref-type="bibr">Freundt et al., 2010</xref>), indicating the formation of late endosomes. Expression of p3a is also required for the Golgi fragmentation for its acquisition of a viral envelope. Through yeast-2-hybrid and FRET assays, Padhan et al. have demonstrated caveolin-1 binding to p3a, which may play a role in virus uptake and release (<xref rid="b0285" ref-type="bibr">Padhan et al., 2007</xref>).p3a has also been associated with ER stress through the activation of the PERK pathway but not the IRE-1 and ATF6 pathway (<xref rid="b0290" ref-type="bibr">Minakshi et al., 2009</xref>). Minakshi et al. analyzed this by transfecting Huh7 cells with p3a and luciferase-tagged ER stress-related molecules, showing that it can enhance protein folding, but not activate Endoplasmic Reticulum Associated Degradation (ERAD) <xref rid="b0290" ref-type="bibr">Minakshi et al., 2009</xref>. The long-term triggering of the PERK pathway can also induce virus-related apoptosis through the expression of its downstream mediators of ATF4 and CHOP, similar to IBV infection (<xref rid="b0800" ref-type="bibr">Liao et al., 2013</xref>). Padhan et al. have shown that protein 3a can lead to increased activation of the p38 MAP kinase pathway and induce the mitochondria to leak cytochrome c to induce apoptosis (<xref rid="b0300" ref-type="bibr">Padhan et al., 2008</xref>).</p></sec><sec id="s0020"><label>2.2</label><title>Orf3b</title><p id="p0075">Protein 3b (p3b) is translated from ORF3 using an IRES (<xref rid="b0095" ref-type="bibr">Rota et al., 2003</xref>), overlapping the SARS-CoV E gene as well as the ORF3a. Similarly to the SARS-CoV p3a, p3b does not share any homology with other known proteins (<xref rid="b0090" ref-type="bibr">Marra et al., 2003</xref>). Moreover, p3b is not expressed in SARS-CoV isolates from bats due to the presence of a stop codon (<xref rid="b0305" ref-type="bibr">Ren et al., 2006</xref>). Anti-p3b antibodies can also be detected in the sera of infected SARS patients (<xref rid="b0310" ref-type="bibr">Guo et al., 2004</xref>). Yuan et al. have shown using immunofluorescence that the SARS-CoV p3b is localized in the nucleolus of infected Vero E6 cells (<xref rid="b0165" ref-type="bibr">Yuan et al., 2005</xref>) as well as in the mitochondria (<xref rid="b0315" ref-type="bibr">Yuan et al., 2006</xref>, <xref rid="b0320" ref-type="bibr">Freundt et al., 2009</xref>). The shuttling behavior may be due to the presence of a nuclear export sequence and determined by a leptomycin B-sensitive mechanism. Furthermore, involvement of p3b has also been demonstrated in the induction of necrosis and apoptosis independent of its subcellular localization (<xref rid="b0325" ref-type="bibr">Khan et al., 2006</xref>, <xref rid="b0330" ref-type="bibr">Yuan et al., 2005</xref>). p3b is also able to inhibit the antiviral response by down-regulating type-I interferon (IFN-β) as well as the mitochondrial antiviral response (<xref rid="b0320" ref-type="bibr">Freundt et al., 2009</xref>, <xref rid="b0335" ref-type="bibr">Spiegel et al., 2005</xref>). Using flow cytometry, Yuan et al., showed that COS-7 cells transfected with p3b are arrested at the G0/G1 phase (<xref rid="b0330" ref-type="bibr">Yuan et al., 2005</xref>). The protein may play a role in immunomodulation through binding to RUNX1b, as seen from yeast-two-hybrid and co-immunoprecipitation by Varshney et al. <xref rid="b0340" ref-type="bibr">Varshney et al. (2012)</xref>. Additionally, p3b can act as an interferon antagonist through inhibition of IRF3 (<xref rid="b0345" ref-type="bibr">Kopecky-Bromberg et al., 2007</xref>) and activation of AP-1 through upregulating the expression levels of JNK and ERK (<xref rid="b0350" ref-type="bibr">Varshney and Lal, 2011</xref>) (see <xref rid="t0010" ref-type="table">Table 2</xref>).<table-wrap position="float" id="t0010"><label>Table 2</label><caption><p>SARS-CoV ORF3b.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p3b</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0020"><list-item id="o0080"><label>•</label><p id="p0335">Subcellular localization: nucleolus, mitochondria</p></list-item><list-item id="o0085"><label>•</label><p id="p0340">Minor structural protein</p></list-item><list-item id="o0090"><label>•</label><p id="p0345">Not required for SARS-CoV replication</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0025"><list-item id="o0095"><label>■</label><p id="p0350">Full length p3b: inhibits type I interferon response and mitochondrial antiviral response</p></list-item><list-item id="o0100"><label>■</label><p id="p0355">Full length p3b: G0/G1 cell cycle arrest</p></list-item><list-item id="o0105"><label>■</label><p id="p0360">Full length p3b: interacts with RUNX1b, immunomodulation</p></list-item><list-item id="o0110"><label>■</label><p id="p0365">Full length p3b: activate AP-1 through JNK/ERK pathway</p></list-item><list-item id="o0115"><label>■</label><p id="p0370">Full length p3b: induces necrosis and apoptosis</p></list-item></list></td></tr></tbody></table></table-wrap></p></sec><sec id="s0025"><label>2.3</label><title>Orf6</title><p id="p0080">Protein 6 (p6) is detected in lung and ileum tissues of patients and in SARS-CoV-infected Vero E6 cells (<xref rid="b0355" ref-type="bibr">Geng et al., 2005</xref>). The protein is found to associate with cellular membranes and is mainly localized in the ER and Golgi apparatus, either when expressed via a recombinant murine coronavirus or when it is transfected as an EGFP-fusion protein (<xref rid="b0355" ref-type="bibr">Geng et al., 2005</xref>, <xref rid="b0360" ref-type="bibr">Pewe et al., 2005</xref>). By sucrose gradient centrifugation and a virus-capture assay, Huang et al. have demonstrated that p6 is incorporated into mature virions (<xref rid="b0365" ref-type="bibr">Huang et al., 2007</xref>). Moreover, when co-expressed with SARS-CoV S, M, and E proteins, p6 is incorporated into VLPs. However, p6 is not detected in the virions when expressed via a recombinant murine coronavirus (MHV) (<xref rid="b0360" ref-type="bibr">Pewe et al., 2005)</xref>. These results indicate that specific physical interactions between p6 and other SARS-CoV structural proteins may be required for recruiting p6 into the virus particles (see <xref rid="t0015" ref-type="table">Table 3</xref>).<table-wrap position="float" id="t0015"><label>Table 3</label><caption><p>SARS-CoV ORF6.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p6</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0030"><list-item id="o0120"><label>•</label><p id="p0375">Subcellular localization: ER and Golgi</p></list-item><list-item id="o0125"><label>•</label><p id="p0380">Minor structural protein</p></list-item><list-item id="o0130"><label>•</label><p id="p0385">Not required for SARS-CoV replication, but contribute to virulence</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0035"><list-item id="o0135"><label>■</label><p id="p0390">Amphipathic N-terminal (aa 2–37): induces membrane rearrangement and the formation of double membrane vesicles</p></list-item><list-item id="o0140"><label>■</label><p id="p0395">C-terminal (aa 54–63): critical for KPNA2 binding and inhibition of STAT1 nuclear import in response to interferon signaling</p></list-item><list-item id="o0145"><label>■</label><p id="p0400">Entire p6 protein: stimulates DNA synthesis</p></list-item><list-item id="o0150"><label>■</label><p id="p0405">Entire p6 protein: suppresses the expression of co-transfected plasmids</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0085">Initial reverse genetic studies by Yount et al. have shown that recombinant SARS-CoV with ORF6 deleted (rSARS-CoV-Δ6) replicates similarly to wild-type virus in cell cultures as well as in infected BALB/c mice (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>). However, a later study by Zhao et al. has demonstrated that, at a low MOI (0.01), rSARS-CoV-Δ6 replicates slower and to lower titres in cell cultures (<xref rid="b0370" ref-type="bibr">Zhao et al., 2009</xref>). Compared with wild-type virus, lower viral titres as well as lower levels of viral RNA and protein were observed for rSARS-CoV-Δ6 at the early stage of infection, but the differences gradually diminished as the infection progressed. Importantly, when transgenic mice expressing the SARS-CoV receptor hACE2 were used, rSARS-CoV-Δ6-infected mice demonstrated a lower morbidity and mortality, with slightly lower virus titres in lung and brain tissue compared with wild-type virus (<xref rid="b0370" ref-type="bibr">Zhao et al., 2009</xref>). Moreover, when ORF6 was introduced into an otherwise sub-lethal strain of MHV, the recombinant virus displayed enhanced growth in cell cultures and resulted in lethal encephalitis in infected mice (<xref rid="b0360" ref-type="bibr">Pewe et al., 2005</xref>, <xref rid="b0375" ref-type="bibr">Tangudu et al., 2007</xref>). Taken together, these results indicate that SARS-CoV p6 enhances viral replication <italic>in vitro</italic> and <italic>in vivo</italic>, and it may serve an important role in the pathogenesis during SARS-CoV infection.</p><p id="p0090">SARS-CoV protein 6 is a 63-aa polypeptide with an amphipathic N-terminal portion (aa 1–40) and a highly polar C-terminal portion. An initial study using GFP-fusion protein and digitonin permeabilization supported an N-endo, C-endo membrane topology (<xref rid="b0380" ref-type="bibr">Netland et al., 2007</xref>) (<xref rid="f0015" ref-type="fig">Fig. 3</xref>). Later, it was confirmed that residues 2–37 in the N-terminal region form an α-helix and are embedded in the cellular membrane (<xref rid="b0370" ref-type="bibr">Zhao et al., 2009</xref>). On the other hand, the C-terminal part contains two signal sequences. The sequence YSEL (aa 49–52) is known to target proteins for internalization into endosomes, while the acidic tail signals ER export (<xref rid="f0010" ref-type="fig">Fig. 2</xref>) (<xref rid="b0380" ref-type="bibr">Netland et al., 2007</xref>).</p><p id="p0095">Utilizing the recombinant MHV expressing SARS-CoV p6 as mentioned above, Netland et al. demonstrated that the enhancement effect of p6 is dependent on the N-terminal amphipathic region but not the C-terminal polar region (<xref rid="b0380" ref-type="bibr">Netland et al., 2007</xref>). Using transmission electron microscopy, Zhou et al. showed that full-length p6 or the N-terminal domain modified the cellular membrane, producing perinuclear vesicles that resemble the double-membrane vesicles (DMVs) known to be associated with coronavirus replication (<xref rid="b0385" ref-type="bibr">Zhou et al., 2010</xref>). The ability to modify intracellular membranes may also explain the observation that SARS-CoV p6 induces ER stress in transfected cells (<xref rid="b0390" ref-type="bibr">Ye et al., 2008</xref>). The p6-induced perinuclear vesicles, similar to the virus-induced DMVs, may derive and reorganize the membrane from the ER, thus leading to perturbation of ER function and induction of ER stress (<xref rid="b0390" ref-type="bibr">Ye et al., 2008</xref>, <xref rid="b0395" ref-type="bibr">Knoops et al., 2008</xref>). Notably, p6 is found to partially co-localize with nonstructural protein 3 (nsp3), which contains the papain-like viral protease and is a marker of the replication complex, as well as nonstructural protein 8 (nsp8), the primer-independent, non-canonical RNA polymerase (<xref rid="b0375" ref-type="bibr">Tangudu et al., 2007</xref>, <xref rid="b0385" ref-type="bibr">Zhou et al., 2010</xref>, <xref rid="b0400" ref-type="bibr">Kumar et al., 2007</xref>). Moreover, physical interaction between p6 and nsp8 in SARS-CoV-infected Vero E6 cells has been confirmed by Kumar et al. using yeast two-hybrid and co-immunoprecipitation (<xref rid="b0400" ref-type="bibr">Kumar et al., 2007</xref>). These results indicate that the N-terminal domain of p6 may play a role in the formation of the replication complex in SARS-CoV-infected cells and serve auxiliary or modulatory functions during SARS-CoV genome replication.</p><p id="p0100">Protein 6 was identified as a β-interferon antagonist by Kopecky-Bromberg et al. with the intriguing finding that overexpression of p6 inhibits nuclear import of STAT1 in cells treated with IFN-β (<xref rid="b0345" ref-type="bibr">Kopecky-Bromberg et al., 2007</xref>). Further studies by the same group revealed that p6 can physically interact with karyopherin alpha 2 (KPNA2) and tether it to the rough ER (<xref rid="b0070" ref-type="bibr">Frieman et al., 2007</xref>). The immobilized KPNA2 interacts with karyopherin beta 1 (KPNB1) and prevents it from forming a complex with STAT1 to facilitate nuclear import (<xref rid="b0370" ref-type="bibr">Zhao et al., 2009</xref>). The KPNA2-interacting region in p6 is mapped to 10 aa in the C-terminal region (<xref rid="b0070" ref-type="bibr">Frieman et al., 2007</xref>). Later, Hussain et al. confirmed that p6 but not its C-terminally truncated mutant (p6ΔC) impeded the nuclear import of proteins harboring a classical nuclear localization signal (NLS) (<xref rid="b0410" ref-type="bibr">Hussain et al., 2008</xref>). Further studies also demonstrated that the C-terminal tail is necessary but not sufficient to impede nuclear import, and a lipophilic N-terminus context is required (<xref rid="b0415" ref-type="bibr">Hussain and Gallagher, 2010</xref>). Taken together, these results point to a role of the C-terminal domain of p6 in modulating host protein nuclear transport and type-I interferon signaling, which may be important for immune evasion during SARS-CoV infection.</p><p id="p0105">One recent study has demonstrated a physical interaction and co-localization between SARS-CoV p6 and another accessory protein, 9b (<xref rid="b0420" ref-type="bibr">Calvo et al., 2012</xref>). Other studies have also shown that when overexpressed, SARS-CoV p6 can stimulate DNA synthesis in cell culture and suppress the expression of co-transfected constructs (<xref rid="b0355" ref-type="bibr">Geng et al., 2005</xref>, <xref rid="b0410" ref-type="bibr">Hussain et al., 2008</xref>, <xref rid="b0425" ref-type="bibr">Gunalan et al., 2011</xref>). The physiological significances of these findings in SARS-CoV replication and pathogenesis are currently unknown.</p></sec><sec id="s0030"><label>2.4</label><title>Orf7a</title><p id="p0110">SARS-CoV accessory protein 7a (p7a, also known as ORF8, U122, or X4) is detected in SARS-CoV-infected Vero E6 cells and in lung specimens from SARS patients (<xref rid="b0430" ref-type="bibr">Chen et al., 2005</xref>, <xref rid="b0435" ref-type="bibr">Fielding et al., 2004</xref>, <xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>). The protein is localized in the ER and ER-Golgi intermediate compartment (ERGIC) in SARS-CoV-infected cells and in transfected cells (<xref rid="b0435" ref-type="bibr">Fielding et al., 2004</xref>, <xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>). Using sucrose gradient centrifugation and a virus capture assay, Huang et al. have shown that SARS-CoV p7a is a structural protein incorporated into mature virions (<xref rid="b0445" ref-type="bibr">Huang et al., 2006</xref>). Although p7a physically interacts with the SARS-CoV S protein and accessory protein 3a, neither S nor p3a is required for recruiting p7a into the virus particle; since p7a-containing VLPs can be produced in cells expressing only SARS-CoV M, E, and 7a (<xref rid="b0160" ref-type="bibr">Tan et al., 2004</xref>, <xref rid="b0445" ref-type="bibr">Huang et al., 2006</xref>) (see <xref rid="t0020" ref-type="table">Table 4</xref>).<table-wrap position="float" id="t0020"><label>Table 4</label><caption><p>SARS-CoV ORF7a.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p7a</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0040"><list-item id="o0155"><label>•</label><p id="p0410">Subcellular localization: ER and ER-Golgi intermediate compartment</p></list-item><list-item id="o0160"><label>•</label><p id="p0415">Minor structural protein</p></list-item><list-item id="o0165"><label>•</label><p id="p0420">Not required for SARS-CoV replication</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0045"><list-item id="o0170"><label>■</label><p id="p0425">N-terminal (aa 1–15): signal peptide</p></list-item><list-item id="o0175"><label>■</label><p id="p0430">Luminal domain (aa 16–96): resembles ICAM-1-fold, bind to LFA-1</p></list-item><list-item id="o0180"><label>■</label><p id="p0435">Ser44 to Val82: essential for cytoplasmic localization and cell cycle arrest</p></list-item><list-item id="o0185"><label>■</label><p id="p0440">Transmembrane domain (aa 98–116): required for interaction with Bcl-X<sub>L</sub> and the induction of apoptosis</p></list-item><list-item id="o0190"><label>■</label><p id="p0445">C-terminal tail (aa 118–122): ER retention signal</p></list-item><list-item id="o0195"><label>■</label><p id="p0450">Entire p7a protein: activates NF-κB and JNK for IL-8 and RANTES production</p></list-item><list-item id="o0200"><label>■</label><p id="p0455">Entire p7a protein: activates p38 and inhibits translation of cellular proteins</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0115">An early study based on reverse genetics has shown that deletion of ORF7a from the SARS-CoV genome does not significantly affect viral RNA synthesis and replication efficiency in cell culture (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>). Schaecher et al. have also found that deletion of both ORF7a and 7b does not significantly affect SARS-CoV replication in transformed cells and in infected golden Syrian hamsters (<xref rid="b0450" ref-type="bibr">Schaecher et al., 2007</xref>, <xref rid="b0455" ref-type="bibr">Pekosz et al., 2006</xref>). Later, Dediego et al. confirmed that recombinant SARS-CoV with ORF6, 7a, 7b, 8a, 8b, and 9b deleted produced viral particles with similar morphology as wild-type SARS-CoV and replicated similarly in transgenic mice expressing the SARS-CoV receptor (<xref rid="b0460" ref-type="bibr">DeDiego et al., 2008</xref>). These results indicate that p7a is not essential for SARS-CoV replication <italic>in vitro</italic> and <italic>in vivo</italic>.</p><p id="p0120">SARS-CoV p7a is a type-I transmembrane protein with 122 amino-acid residues in length (<xref rid="f0015" ref-type="fig">Fig.3</xref>). The 15 N-terminal residues constitute a signal peptide, which is cleaved by signal peptidase in both SARS-CoV-infected cells and in transfected cells (<xref rid="b0435" ref-type="bibr">Fielding et al., 2004</xref>, <xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>). The aa residues 16–96 comprise the luminal domain, which folds into a compact seven-stranded β sandwich that resembles members of the Ig superfamily (<xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>). The sequence and structural homology between SARS-CoV p7a and ICAM-1 suggested that p7a may interact with the lymphocyte function-associated antigen 1 (LFA-1), which was later confirmed by direct <italic>in vitro</italic> binding experiments in Jurkat cells (<xref rid="b0465" ref-type="bibr">Hänel et al., 2006</xref>, <xref rid="b0470" ref-type="bibr">Hänel and Willbold, 2007</xref>). These data suggest LFA-1 to be an attachment factor or even receptor for SARS-CoV on human leukocytes, although a majority of p7a is found to remain intracellular in SARS-CoV-infected cells (<xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>, <xref rid="b0470" ref-type="bibr">Hänel and Willbold, 2007</xref>). Residues 97–117 of SARS-CoV p7a are highly hydrophobic and transverse the cellular membrane. The last 5 residues of the C-terminal tail (KRKTE) form a typical ER retention motif (KKXX or KXKXX, where X is any amino acid). When this motif is mutated from KRKTE to ERETE, p7a fails to be retro-transported to the ER and accumulates in the Golgi, where it undergoes rapid proteolytic processing (<xref rid="b0435" ref-type="bibr">Fielding et al., 2004</xref>). Moreover, when the transmembrane domain and the short C-terminal tail of SARS-CoV p7a are fused with the cell-surface protein CD4, the chimera protein (CD4/orf7a-TM-tail) is found to be partially retained in the Golgi (<xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>). Fielding et al. have demonstrated physical interaction between SARS-CoV p7a and the host protein small glutamine-rich tetratricopeptide repeat-containing protein (SGT) (<xref rid="b0475" ref-type="bibr">Fielding et al., 2006</xref>). Previous studies have shown that SGT interacts with the HIV-1 accessory protein vpu, which is essential in modulating viral particle release (<xref rid="b0480" ref-type="bibr">Bour and Strebel, 2003</xref>). These results, together with the fact that SARS-CoV p7a is localized in the ER and ERGIC where coronaviruses generally assemble, and that p7a physically interacts with SARS-CoV M and E proteins, indicate that p7a may serve a function in the assembly stage during SARS-CoV replication (<xref rid="b0475" ref-type="bibr">Fielding et al., 2006</xref>).</p><p id="p0125">When transfected in mammalian cells, SARS-CoV p7a has been shown to induce caspase-dependent apoptosis as evidenced by the cleavage of poly(ADP-ribose) polymerase (PARP), a well-characterized apoptotic marker (<xref rid="b0485" ref-type="bibr">Tan et al., 2004</xref>). This is consolidated by the finding that recombinant SARS-CoV with 7a and 7b deleted is not as efficient as wild-type virus in inducing DNA fragmentation, although the induction of early apoptotic markers such as annexin-V binding and caspase-3 activation are similar (<xref rid="b0450" ref-type="bibr">Schaecher et al., 2007</xref>). These results indicate that SARS-CoV-induced apoptosis is only partially contributed by p7a, and other viral factors may be involved in the process. Later, Tan et al. demonstrated that the pro-apoptotic property of SARS-CoV p7a depends on its transmembrane domain, which is also required for its interaction with Bcl-X<sub>L</sub>, an anti-apoptotic protein belonging to the Bcl-2 family (<xref rid="b0490" ref-type="bibr">Tan et al., 2007</xref>). Interestingly, SARS-CoV p7a interacts strongly with the pro-survival members of the Bcl-2 family (such as Bcl-X<sub>L</sub>, Bcl-w, Mcl-1, and A1), but not the pro-apoptotic Bcl-2 family proteins (such as BAD, BID, BAX and BAK) (<xref rid="b0490" ref-type="bibr">Tan et al., 2007</xref>). The selective interactions may hinder the pro-survival functions of these proteins and result in apoptosis induction in SARS-CoV-infected cells.</p><p id="p0130">When overexpressed, protein 7a can inhibit the growth of Balb/c 3T3 cells in a dose-dependent and time-dependent manner (<xref rid="b0430" ref-type="bibr">Chen et al., 2005</xref>). A later study by Yuan et al. confirmed that overexpression of 7a led to cell cycle arrest at the G0/G1 phase, possibly by inhibiting the phosphorylation of retinoblastoma (Rb) protein (<xref rid="b0495" ref-type="bibr">Yuan et al., 2006</xref>). Deletion-mutant experiments narrowed down the domain spanning aa 44–82 to be essential for cytoplasmic localization and cell-cycle arrest (<xref rid="b0495" ref-type="bibr">Yuan et al., 2006</xref>). Overexpression of 7a has also been shown to activate nuclear factor kappa B (NF-κB) and c-Jun N-terminal kinase (JNK), which in turn induce the production of pro-inflammatory cytokines such as interleukin 8 (IL-8) and RANTES (<xref rid="b0145" ref-type="bibr">Kanzawa et al., 2006</xref>). Moreover, using EGFP fusion protein, Kopecky-Bromberg et al. showed that overexpression of 7a activates the p38 mitogen-activated protein kinase (MAPK) and inhibits cellular protein synthesis at the translation level (<xref rid="b0500" ref-type="bibr">Kopecky-Bromberg et al., 2006</xref>). However, p7a-induced apoptosis is not inhibited in the presence of a p38 inhibitor, indicating that p7a induces apoptosis via mechanisms independent of the MAPK pathways (<xref rid="b0500" ref-type="bibr">Kopecky-Bromberg et al., 2006</xref>).</p><p id="p0135">Taken together, although SARS-CoV p7a is not essential for viral replication, it is a minor structural protein and may serve certain functions during viral assembly. Notably, p7a is actively involved in virus–host interaction. By inhibiting cellular translation, activating stress-induced MAP kinases, suppressing cell-cycle progression and inducing caspase-dependent apoptosis, p7a may be an important player in SARS-CoV pathogenesis.</p></sec><sec id="s0035"><label>2.5</label><title>Orf7b</title><p id="p0140">Accessory protein 7b was predicted to be translated from a second ORF of SARS-CoV sgRNA7 (<xref rid="b0505" ref-type="bibr">Snijder et al., 2003</xref>). The expression of 7b was later confirmed in Vero cells infected with SARS-CoV (<xref rid="b0510" ref-type="bibr">Schaecher et al., 2007</xref>). No experiments have been performed to detect the expression of 7b in tissue samples from SARS-CoV patients, although the presence of anti-p7b antibodies in SARS-convalescent patient sera indicates that the 7b protein is likely expressed <italic>in vivo</italic> (<xref rid="b0310" ref-type="bibr">Guo et al., 2004</xref>). Using sucrose gradient fractionation and immune-gold labeling, Schaecher et al. demonstrated that p7b is associated with intracellular viruses and incorporated into purified virions, although more stringent virus-capture assays have not been performed (<xref rid="b0510" ref-type="bibr">Schaecher et al., 2007</xref>) (see <xref rid="t0025" ref-type="table">Table 5</xref>).<table-wrap position="float" id="t0025"><label>Table 5</label><caption><p>SARS-CoV ORF7b.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p7b</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0050"><list-item id="o0205"><label>•</label><p id="p0460">Subcellular localization: Golgi compartment</p></list-item><list-item id="o0210"><label>•</label><p id="p0465">Minor structural protein</p></list-item><list-item id="o0215"><label>•</label><p id="p0470">Not required for SARS-CoV replication. But may be an attenuating factor <italic>in vivo</italic></p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0055"><list-item id="o0220"><label>■</label><p id="p0475">Transmembrane domain (aa 9–29): required for Golgi localization</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0145">SARS-CoV 7b is proposed to be translated via a ribosome leaky scanning mechanism, because the expression of 7b is significantly reduced when the upstream 7a start codon is mutated to a strong Kozak sequence or when an additional AUG is introduced upstream of the 7b start codon (<xref rid="b0510" ref-type="bibr">Schaecher et al., 2007</xref>). Protein 7b is a 44-aa, highly hydrophobic polypeptide (<xref rid="b0510" ref-type="bibr">Schaecher et al., 2007</xref>). It is an integral transmembrane protein with a luminal N-terminus and a cytoplasmic C-terminus (<xref rid="f0015" ref-type="fig">Fig. 3</xref>). Similar to p7a, the 7b protein is localized throughout the Golgi compartment in both SARS-CoV-infected cells and in cells transfected with 7b cDNA (<xref rid="b0510" ref-type="bibr">Schaecher et al., 2007</xref>). The Golgi-restricted localization of p7b was attributed to the transmembrane domain, specifically aa 21–23 and 27–30 of p7b (<xref rid="b0515" ref-type="bibr">Schaecher et al., 2008</xref>). Notably, when the Golgi-targeting sequence of SARS-CoV p7b is used to replace the transmembrane domain of the cell-surface protein CD4, the chimeric protein is retained in the Golgi complex (<xref rid="b0515" ref-type="bibr">Schaecher et al., 2008</xref>). Thus, the transmembrane domain of p7b is both necessary and sufficient for its Golgi localization.</p><p id="p0150">Various reverse genetic experiments have shown that the SARS-CoV ORF7b is not essential for viral replication <italic>in vitro</italic> and <italic>in vivo</italic> (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>, <xref rid="b0450" ref-type="bibr">Schaecher et al., 2007</xref>, <xref rid="b0460" ref-type="bibr">DeDiego et al., 2008</xref>). Interestingly, a prototype virus (strain Frankfurt-I) isolated during the 2003 SARS outbreak has a 45-nt deletion in the transmembrane domain of ORF7b upon propagation in cell culture for 3 passages (<xref rid="b0020" ref-type="bibr">Thiel et al., 2003</xref>). Pfefferle et al. have performed extensive investigations on the consequence of this deletion using reverse genetics (<xref rid="b0520" ref-type="bibr">Pfefferle et al., 2009</xref>). It was found that compared with the parental virus, the recombinant SARS-CoV with ORF7b deleted had a replicative advantage in Caco-2 and Huh7 cells, but not in Vero cells (<xref rid="b0520" ref-type="bibr">Pfefferle et al., 2009</xref>). Moreover, the virus titre and viral RNA levels were significantly higher in Syrian Golden Hamsters infected with the 7b-deleted virus, indicating that SARS-CoV p7b may be an attenuating factor <italic>in vivo</italic> (<xref rid="b0520" ref-type="bibr">Pfefferle et al., 2009</xref>). In a different approach, Åkerström et al. showed that siRNA specific for SARS-CoV sgRNA7 can inhibit sgRNA7 and sgRNA8 (thus silencing the expression of 7a, 7b, 8a, 8b) without affecting the full-length genomic mRNA and other sgRNAs (<xref rid="b0275" ref-type="bibr">Åkerström et al., 2007</xref>). In Vero E6 cells expressing this siRNA, the production of progeny viruses was significantly reduced, indicating that p7a/p7b (and p8a/p8b) may play certain roles during the replication cycle of SARS-CoV. The discrepancies regarding the functions of p7b during SARS-CoV replication may be due to the different systems and animal models used. Further studies are required to validate these results and characterize the detailed mechanisms.</p></sec><sec id="s0040"><label>2.6</label><title>ORF8a and ORF8b</title><p id="p0155">In SARS-CoV isolated from animals and early human isolates, sgRNA8 encodes a single protein 8ab. Interestingly, in SARS-CoV isolated from humans during the peak of the epidemic, there is a 29-nt deletion in the middle of ORF8, resulting in the splitting of ORF8 into two smaller ORFs, namely ORF8a and ORF8b (<xref rid="b0525" ref-type="bibr">He et al., 2004</xref>, <xref rid="b0530" ref-type="bibr">Guan et al., 2003</xref>). During the late stage of the SARS epidemic, larger deletions (82-nt and 415-nt deletion) were also identified in clusters of viruses from human isolates (<xref rid="b0525" ref-type="bibr">He et al., 2004</xref>, <xref rid="b0785" ref-type="bibr">Chiu et al., 2005</xref>). Initially, this dramatic genomic change in ORF8 has been proposed to contribute to the zoonotic transition of SARS-CoV from palm civets to humans and thus has attracted particular attention. However, it has been shown that palm civets are equally susceptible to human SARS-CoV isolates with or without the 29-nt deletion (<xref rid="b0540" ref-type="bibr">Wu et al., 2005</xref>). Moreover, further studies using reverse genetics showed that the ORF8 of SARS-CoV is not essential for virus replication <italic>in vitro</italic> and <italic>in vivo</italic> (<xref rid="b0040" ref-type="bibr">Yount et al., 2005</xref>, <xref rid="b0460" ref-type="bibr">DeDiego et al., 2008</xref>). Thus, whether the mutations in ORF8 are due to genomic instability or adaptive evolution remains to be investigated (see <xref rid="t0030" ref-type="table">Table 6</xref>).<table-wrap position="float" id="t0030"><label>Table 6</label><caption><p>SARS-CoV ORF8a and ORF8b.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p8a, p8b, and p8ab</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0060"><list-item id="o0225"><label>•</label><p id="p0480">Subcellular localization<list list-type="simple" id="l0065"><list-item id="o0230"><label>■</label><p id="p0485">p8a: cytoplasmic punctuated vesicle-like structures, reticular patterns in the ER</p></list-item><list-item id="o0235"><label>■</label><p id="p0490">p8b: cytoplasmic punctuated vesicle-like structures, diffusely in both the cytosol and the nucleus, diffusely in the cytoplasm</p></list-item><list-item id="o0240"><label>■</label><p id="p0495">p8ab: diffusely in the cytoplasm, reticular patterns in the ER</p></list-item></list></p></list-item><list-item id="o0245"><label>•</label><p id="p0500">Not required for SARS-CoV replication</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0070"><list-item id="o0250"><label>■</label><p id="p0505">N-terminal (aa 1–15) in p8a and p8ab: signal peptide</p></list-item><list-item id="o0255"><label>■</label><p id="p0510">Asn81 in p8ab: N-glycosylated, protects p8ab from proteasomal degradation</p></list-item><list-item id="o0260"><label>■</label><p id="p0515">Entire p8a protein: induces caspase-dependent apoptosis</p></list-item><list-item id="o0265"><label>■</label><p id="p0520">Entire p8b protein: stimulates cellular DNA synthesis</p></list-item><list-item id="o0270"><label>■</label><p id="p0525">Entire p8b and p8ab protein: undergo ubiquitination. Interact with both monoubiquitin and polyubiquitin</p></list-item><list-item id="o0275"><label>■</label><p id="p0530">Entire p8ab protein: activates the ATF6 branch of unfolded protein response</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0160">The undeleted ORF8 (or ORF8ab) encodes a 122-amino-acid protein with an N-terminal hydrophobic signal sequence. ORF8a encodes a 39-amino-acid polypeptide, and residues 1–35 are identical to the N-terminal of 8ab. ORF8b encodes an 84-aa polypeptide and residues 9–84 are identical to the C-terminal of 8ab (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>). Whereas Keng et al. have demonstrated by indirect immunofluorescence that 8a and 8b are expressed in SARS-CoV-infected Vero E6 cells, Oostra et al. were unable to detect 8b in SARS-CoV-infected Vero E6 cells (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>, <xref rid="b0550" ref-type="bibr">Keng et al., 2006</xref>). Although there is no data regarding the expression of 8a or 8b <italic>in vivo</italic>, Chen et al. have identified anti-p8a antibodies in two out of 37 patients with SARS (<xref rid="b0555" ref-type="bibr">Chen et al., 2007</xref>). Also, different subcellular localizations have been reported for p8a, p8b, and p8ab. Using an EGFP fusion protein, Keng et al. have shown that p8a and p8b are found in cytoplasmic punctuated vesicle-like structures, whereas p8ab localizes diffusely to the cytoplasm (<xref rid="b0550" ref-type="bibr">Keng et al., 2006</xref>). On the other hand, Law et al. have observed EGFP-fused p8b to localize diffusely in both the cytosol and the nucleus (<xref rid="b0560" ref-type="bibr">Law et al., 2006</xref>). Finally, using a T7 promoter-driven expression system, Oostra et al. have demonstrated a reticular pattern and ER localization for p8a and p8ab, but a diffuse cytoplasmic localization for p8b (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>). These discrepancies should be addressed, possibly by using more specific monoclonal antibodies, or expressing 8a and 8b fusion proteins under a more physiologically relevant background, such as using recombinant coronaviruses.</p><p id="p0165">Oostra et al. have characterized the biochemical properties of p8a, p8b, and p8ab (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>). Owing to its N-terminal signal sequence, SARS-CoV p8ab enters the ER, where it is N-glycosylated at asparagine residue 81. The mature p8ab remains associated with the ER and possibly undergoes multimerization, but it is not transported to the Golgi for secretion (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>). Inside the ER, the protein up-regulates the synthesis of ER chaperons and activates the ATF6 branch of unfolded-protein response (UPR), but shows no effect on the PERK and IRE1 branches of the UPR (<xref rid="b0565" ref-type="bibr">Sung et al., 2009</xref>). The 29-nt deletion disrupts the functional expression of ORF8. Although ORF8a contains the signal sequence, the protein is too short to be transported into the ER. For ORF8b, due to the lack of a signal sequence, it cannot enter the ER and thus is not glycosylated at the same asparagine residue as in 8ab (<xref rid="b0545" ref-type="bibr">Oostra et al., 2007</xref>).</p><p id="p0170">Proteins 8b and 8ab have been shown to undergo ubiquitination when expressed in both <italic>in vitro</italic> expression systems and in cell culture (<xref rid="b0570" ref-type="bibr">Le et al., 2007</xref>). Moreover, p8b and p8ab physically interact with monoubiquitin as well as polyubiquitin, suggesting potential involvement of these proteins in the host ubiquitin–proteasome system. Interestingly, the N-linked glycosylation on residue Asn81 in p8ab stabilizes it and protects it from degradation by the proteasome. In sharp contrast, in the absence of the p8a region, p8b, which is not N-glycosylated at the same asparagine residue, undergoes rapid proteasomal degradation (<xref rid="b0570" ref-type="bibr">Le et al., 2007</xref>).</p><p id="p0175">Chen et al. have shown that SARS-CoV replication is enhanced in cells stably expressing 8a and reduced in cells transfected with siRNA targeting 8a (<xref rid="b0555" ref-type="bibr">Chen et al., 2007</xref>). However, the results were based on detection of virus RNA and assessment of virus-induced CPE only, and a direct measure of infective virus titre should have been performed. Moreover, overexpression of 8a also induces caspase-dependent apoptosis (<xref rid="b0555" ref-type="bibr">Chen et al., 2007</xref>). Ectopic expression of 8b reduces the level of co-transfected SARS-CoV E protein, but not that of other structural proteins. Strikingly, in SARS-CoV-infected cells, the expression of 8b and E are found to be mutually exclusive, indicating a functional antagonism between the two proteins (<xref rid="b0550" ref-type="bibr">Keng et al., 2006</xref>). Similar to SARS-CoV p6, overexpression of 8b has been shown to stimulate DNA synthesis, although co-expression of p6 and 8b does not produce a synergistic effect (<xref rid="b0560" ref-type="bibr">Law et al., 2006</xref>). The significance of these findings in SARS-CoV pathogenesis remains to be determined.</p></sec><sec id="s0045"><label>2.7</label><title>Orf9b</title><p id="p0180">Accessory protein 9b is translated from a second ORF of SARS-CoV sgRNA9 via a ribosomal leaky scanning mechanism (<xref rid="b0575" ref-type="bibr">Xu et al., 2009</xref>). The sgRNA9 also encodes the SARS-CoV N protein, and in fact ORF9b is a complete internal ORF within the N gene in an alternate reading frame (<xref rid="b0575" ref-type="bibr">Xu et al., 2009</xref>). The 9b protein is detected in SARS-CoV-infected cells and the lung and ileum tissues from SARS patients (<xref rid="b0580" ref-type="bibr">Nal et al., 2005</xref>). Moreover, anti-p9b antibodies are also detected in the serum of SARS patients (<xref rid="b0585" ref-type="bibr">Qiu et al., 2005</xref>). These results indicate that SARS-CoV 9b is expressed <italic>in vitro</italic> and <italic>in vivo</italic>. Using sucrose gradient fractionation, Xu et al. have shown that p9b is incorporated into mature virions and packaged into VLPs when co-expressed with E and M proteins (<xref rid="b0575" ref-type="bibr">Xu et al., 2009</xref>). These data indicate that p9b may be a structural component of SARS-CoV virions, but it is also possible that p9b is included during virus assembly due to interaction with E protein, as demonstrated by von Brunn et al. <xref rid="b0170" ref-type="bibr">von Brunn et al. (2007)</xref>. The SARS-CoV 9b protein is also shown to self-interact and interact with nsp5, nsp14, and the accessory protein p6, although the functional significances are not fully understood (<xref rid="b0170" ref-type="bibr">von Brunn et al., 2007</xref>, <xref rid="b0420" ref-type="bibr">Calvo et al., 2012</xref>). Several reverse genetic studies have demonstrated that p9b is not essential for SARS-CoV replication <italic>in vitro</italic> and <italic>in vivo</italic> (<xref rid="b0170" ref-type="bibr">von Brunn et al., 2007</xref>, <xref rid="b0460" ref-type="bibr">DeDiego et al., 2008</xref>) (see <xref rid="t0035" ref-type="table">Table 7</xref>).<table-wrap position="float" id="t0035"><label>Table 7</label><caption><p>SARS Co-V ORF9b.</p></caption><table frame="hsides" rules="groups"><thead><tr><th>SARS-CoV p9b</th></tr></thead><tbody><tr><td><list list-type="simple" id="l0075"><list-item id="o0280"><label>•</label><p id="p0535">Subcellular localization: associated with cytoplasmic vesicular structures</p></list-item><list-item id="o0285"><label>•</label><p id="p0540">Minor structural protein</p></list-item><list-item id="o0290"><label>•</label><p id="p0545">Not required for SARS-CoV replication</p></list-item></list></td></tr><tr><td>
</td></tr><tr><td>Domains and/or regions with characterized functions<list list-type="simple" id="l0080"><list-item id="o0295"><label>■</label><p id="p0550">Amino acids 46–54: nuclear export signal recognized by exportin 1. Nuclear export of 9b facilitates its degradation in the cytoplasm</p></list-item><list-item id="o0300"><label>■</label><p id="p0555">Entire 9b protein: induces caspase-dependent apoptosis</p></list-item></list></td></tr></tbody></table></table-wrap></p><p id="p0185">Protein 9b is a 98-amino-acid polypeptide with no sequence homology to any known proteins. Meier et al. have determined the crystal structure of p9b and shown that the protein has a novel fold with seven β-strands (<xref rid="b0590" ref-type="bibr">Meier et al., 2006</xref>). The β-strands from two molecules form two adjacent twisted β-sheets, resulting in a highly interlocked handshake structure (<xref rid="b0590" ref-type="bibr">Meier et al., 2006</xref>) (<xref rid="f0015" ref-type="fig">Fig. 3</xref>). Interestingly, the structure contains a hydrophobic central cavity, which binds lipid and stabilizes the molecule. Together with its membrane-association nature, this suggested that p9b may have a function during virus assembly (<xref rid="b0590" ref-type="bibr">Meier et al., 2006</xref>).</p><p id="p0190">Protein 9b contains no known NLS and enters the nucleus by passive diffusion (<xref rid="b0595" ref-type="bibr">Sharma et al., 2011</xref>). However, amino-acid residues 46 to 54 (LRLGSQLSL) of p9b belong to a nuclear export signal (NES) motif LX<sub>1-3</sub>LX<sub>2-4</sub>LXL known to be transported by exportin 1 via an energy-dependent mechanism (<xref rid="b0600" ref-type="bibr">Moshynskyy et al., 2007</xref>). Indeed, SARS-CoV p9b has been shown to physically interact with exportin 1 and moreover, mutation of the leucine-rich NES or inhibition of exportin 1 by leptomycin B have been shown to inhibit nuclear export of the 9b protein (<xref rid="b0595" ref-type="bibr">Sharma et al., 2011</xref>, <xref rid="b0600" ref-type="bibr">Moshynskyy et al., 2007</xref>). The nuclear export of p9b facilitates its degradation in the cytoplasm, whereas accumulation of p9b in the nucleus can induce caspase-dependent apoptosis (<xref rid="b0595" ref-type="bibr">Sharma et al., 2011</xref>).</p></sec></sec><sec id="s0050"><label>3</label><title>Accessory proteins of other betacoronaviruses</title><p id="p0195">SARS coronavirus and its likely precursor, bat coronavirus SL-CoV WIV1 (<xref rid="b0790" ref-type="bibr">Ge et al., 2013</xref>), as well as bat coronavirus HKU3 belong to lineage b of the genus <italic>Betacoronavirus (</italic><xref rid="b0605" ref-type="bibr">Lau et al., 2005</xref>). Lineage a (see <xref rid="f0020" ref-type="fig">Fig. 4</xref>for a phylogenetic tree) comprises a number of viruses that probably did not originate from bat coronavirus precursors, such as Bovine Coronavirus (BCoV), Murine Coronavirus (Mouse Hepatitis Virus, MHV), Human Coronavirus HKU1, and Human Coronavirus OC43. The genomes of BCoV, MHV, and Human Coronavirus OC43 encode the accessory protein NS2 (See <xref rid="f0025" ref-type="fig">Fig. 5</xref>), which, at least in case of MHV, has been shown to have phosphodiesterase activity specific for 2′,5′-oligoadenylate (2-5A) (<xref rid="b0610" ref-type="bibr">Zhao et al., 2011</xref>, <xref rid="b0615" ref-type="bibr">Zhao et al., 2012</xref>). Synthesis of the latter by the host enzyme oligo-2′,5′-A synthetase (OAS) is induced by double-stranded RNA intermediates occurring during viral replication, and leads to activation of the host’s RNase L, which in turn degrades viral and cellular mRNA (<xref rid="b0620" ref-type="bibr">Silverman, 2007</xref>). As a result, viral replication and protein synthesis are interrupted. Thus, reduction of 2-5A levels by NS2 constitutes a mechanism of subversion of the interferon-inducible OAS – RNase L pathway. Interestingly, this process is organ- and cell-type specific; NS2 is essential for replication of MHV A59 only in the liver, but not in the brain (<xref rid="b0625" ref-type="bibr">Zhao et al., 2013</xref>). This seems to depend on the level of the IFN-induced expression of the OAS gene, which differs between cell types. The NS2 protein belongs to the superfamily of 2H phosphodiesterases that is characterized by the presence of a pair of conserved His-X-Thr/Ser motifs (<xref rid="b0630" ref-type="bibr">Mazumder et al., 2002</xref>).<fig id="f0020"><label>Fig. 4</label><caption><p>Phylogenetic analysis of coronaviruses based on pp1ab using Kalign multiple sequence alignment and TreeDyn tree viewer (<xref rid="b0770" ref-type="bibr">Chevenet et al., 2006</xref>, <xref rid="b0775" ref-type="bibr">Lassmann and Sonnhammer, 2005</xref>). Next to the branch, the virus name is indicated, along with the previously defined viral lineages, i.e. group 1 for <italic>Alphacoronavirus</italic> members, 2a, 2b, 2c, 2d for <italic>Betacoronavirus</italic> members, and 3 for the member of the genus <italic>Gammacoronavirus</italic>. Scale bar indicates nucleotide substitutions per site.</p></caption><graphic xlink:href="gr4"></graphic></fig><fig id="f0025"><label>Fig. 5</label><caption><p>Genome organization of members of Betacoronavirus lineages a–d, as well as of the genera Alphacoronavirus and Gammacoronavirus, showing structural and accessory genes downstream of the orf1a/1b gene. The 5′ leader sequence (black box), open reading frames (ORFs 1a, 1b) encoding components of the replication/transcription complex, the genes encoding the structural proteins spike (S), membrane (M), envelope (E), and nucleocapsid (N) are also indicated in gray. Interspersed between (or within) them are the genes coding for putative accessory proteins (not drawn to scale). The following coronavirus genomes are illustrated (GenBank accession numbers in brackets): (1) Bovine coronavirus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_003045" id="ir0005">NC_003045</ext-link>), (2) Mouse Hepatitis Virus (AC_000192), (3) Human coronavirus HKU1 (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_006577" id="ir0010">NC_006577</ext-link>), (4) Human coronavirus OC43 (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_005147" id="ir0015">NC_005147</ext-link>), (5) Severe Acute Respiratory Syndrome coronavirus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_004718" id="ir0020">NC_004718</ext-link>), (6) Tylonycteris bat coronavirus HKU4 (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_009019" id="ir0025">NC_009019</ext-link>), (7) Pipistrellus bat coronavirus HKU5 (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_009020" id="ir0030">NC_009020</ext-link>), (8) MERS coronavirus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_019843" id="ir0035">NC_019843</ext-link>), (9) Rousettus bat coronavirus HKU9 (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_009021" id="ir0040">NC_009021</ext-link>). (10) Feline infectious peritonitis virus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_002306" id="ir0045">NC_002306</ext-link>), (11) Porcine respiratory coronavirus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:DQ811787" id="ir0050">DQ811787</ext-link>), (12) Transmissible gastroenteritis virus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:DQ811788" id="ir0055">DQ811788</ext-link>), (13) Human coronavirus 229E (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_002645" id="ir0060">NC_002645</ext-link>), (14) Infectious Bronchitis Virus (<ext-link ext-link-type="uri" xlink:href="ncbi-n:NC_001451" id="ir0065">NC_001451</ext-link>).</p></caption><graphic xlink:href="gr5"></graphic></fig></p><p id="p0200">Betacoronaviruses of lineage a also include a haemagglutinin esterase (HE) gene between orf1ab and the S gene (<xref rid="f0025" ref-type="fig">Fig. 5</xref>). The hemagglutinin-esterases (HEs) are a family of viral envelope glycoproteins that mediate reversible attachment to O-acetylated sialic acids by acting both as receptor-binding (“lectins”) and as receptor-destroying enzymes (RDEs) with esterase activity (<xref rid="b0635" ref-type="bibr">Woo et al., 2010</xref>, <xref rid="b0640" ref-type="bibr">Zhang et al., 1992</xref>, <xref rid="b0645" ref-type="bibr">Vlasak et al., 1988</xref>, <xref rid="b0650" ref-type="bibr">Schultze et al., 1991</xref>). In coronaviruses, this gene is exclusively present in betacoronaviruses of clade a and has probably been acquired after diverging from the ancestors of other betacoronavirus lineages (<xref rid="b0655" ref-type="bibr">Zeng et al., 2008</xref>), possibly via horizontal gene transfer from Influenza C virus (<xref rid="b0660" ref-type="bibr">Langereis et al., 2012</xref>). Crystal structures have been determined for the hemagglutinin esterases of BCoV and MHV (<xref rid="b0645" ref-type="bibr">Vlasak et al., 1988</xref>, <xref rid="b0650" ref-type="bibr">Schultze et al., 1991</xref>). The E domain (with esterase activity) is found to be highly conserved but the RBD (receptor binding domain) is found to be flexible (<xref rid="b0645" ref-type="bibr">Vlasak et al., 1988</xref>). HCoV OC43 and BCoV use 9-O-acetylated sialic acids as receptor to initiate infection, and correspondingly possess sialate-9-O-acetylesterases as RDE. Most betacoronaviruses of lineage a attach to 9-O-acetylated sialic acid, but one group of murine coronaviruses has switched to using 4-O-acetylated sialic acid (<xref rid="b0650" ref-type="bibr">Schultze et al., 1991</xref>). The available crystal structures reveal the adaptations of the RBD to differences in receptor usage (<xref rid="b0645" ref-type="bibr">Vlasak et al., 1988</xref>, <xref rid="b0650" ref-type="bibr">Schultze et al., 1991</xref>).</p><p id="p0205">Betacoronaviruses of lineage a also encode an internal ORF within the nucleocapsid gene (<xref rid="f0025" ref-type="fig">Fig. 5</xref>). This ORF shares with SARS-CoV ORF9b the property of being completely overlapping with the nucleocapsid gene, but the encoded protein appears to have little in common with protein 9b of the SARS virus. A study performed on the internal ORF within the nucleocapsid gene of MHV suggests that this gene encodes a structural protein that is, however, found to be non-essential for virion formation and viral replication (<xref rid="b0665" ref-type="bibr">Fischer et al., 1997</xref>).</p><p id="p0210">In the genomes of betacoronaviruses of lineage c, for example in the bat viruses (BtCoV) HKU4, HKU5, and the recently characterized Middle-East Respiratory Syndrome (MERS) coronavirus, there are four ORFs between the S and E genes, which encode proteins p3 (also known as p3a), p4a (also known as p3b), p4b (also known as p3c), and p5 (also known as p3d) (<xref rid="f0025" ref-type="fig">Fig. 5</xref>). p3 and p4b have been predicted to each contain one transmembrane helix, whereas p5 has been proposed to comprise three such helices (<xref rid="b0670" ref-type="bibr">Siu et al., 2014</xref>). p3 and p5 have been found in the ERGIC, whereas p4a and p4b display a diffuse cytoplasmic distribution and are also partly localized to the nucleus (<xref rid="b0670" ref-type="bibr">Siu et al., 2014</xref>), although in another study, p4b was found to localize exclusively to the nucleus (<xref rid="b0675" ref-type="bibr">Matthews et al., 2014</xref>). Both the p4a and the p4b protein of MERS coronavirus have been shown to be a type-I interferon antagonists (<xref rid="b0670" ref-type="bibr">Siu et al., 2014</xref>, <xref rid="b0675" ref-type="bibr">Matthews et al., 2014</xref>, <xref rid="b0680" ref-type="bibr">Niemeyer et al., 2013</xref>). The p4a is particularly interesting in this context, as it is a double-stranded (ds) RNA-binding protein that prevents dsRNA (which occurs as an intermediate of viral RNA replication) from binding to the cellular dsRNA-binding protein PACT, which, as a consequence, cannot activate the cellular dsRNA sensors RIG-I and MDA5 (<xref rid="b0670" ref-type="bibr">Siu et al., 2014</xref>, <xref rid="b0680" ref-type="bibr">Niemeyer et al., 2013</xref>). In another study, it has been shown that p4a, p4b and p5 are all potent interferon antagonists with p4a being the most powerful in counteracting the antiviral effects of IFN via the inhibition of both interferon production and ISRE (Interferon-Stimulated Response Element) promoter element signaling pathways (<xref rid="b0685" ref-type="bibr">Yang et al., 2013</xref>). MERS coronavirus also contains an internal ORF within the nucleocapsid gene, which has not yet been characterized (<xref rid="b0690" ref-type="bibr">van Boheemen et al., 2012</xref>). This ORF was not previously described for BtCoV-HKU4 and BtCoV-HKU5 but is conserved in the genome sequences of both viruses.</p><p id="p0215">In betacoronavirus lineage d, for example in bat coronavirus HKU9, two ORFs, both of which are connected with a transcription regulatory sequence (TRS), are found downstream of the N gene (<xref rid="f0025" ref-type="fig">Fig. 5</xref>). The TRSs are located at the 5′ end of coronavirus genes and include a highly conserved AU-rich core sequence that is essential for mediating a 100- to 1000-fold increase in mRNA synthesis when it is located in the appropriate context (<xref rid="b0695" ref-type="bibr">Sawicki et al., 2007</xref>). Thus, the identification of TRSs before a predicted ORF helps identify new genes. The two accessory ORFs in bat-coronavirus HKU9 encode two putative non-structural proteins, NS7a and NS7b (<xref rid="b0700" ref-type="bibr">Woo et al., 2007</xref>, <xref rid="b0705" ref-type="bibr">Lau et al., 2010</xref>). This is in fact the first time that ORFs downstream of the N gene have been observed in a betacoronavirus.</p></sec><sec id="s0055"><label>4</label><title>Accessory proteins in <italic>Alphacoronavirus</italic></title><p id="p0220">In the genome of Feline Infectious Peritonitis Virus (FIPV), there are five ORFs coding for five accessory proteins (<xref rid="f0025" ref-type="fig">Fig. 5</xref>). These are the ORF3 proteins (comprising p3a, p3b, and p3c) and the ORF7 proteins, interestingly encoded by two ORFs (7a and 7b) downstream of the N gene. It has been demonstrated that the ORF7 proteins are essential for efficient replication <italic>in vitro</italic> and for virulence <italic>in vivo (</italic><xref rid="b0035" ref-type="bibr">Haijema et al., 2004</xref>). The ORF3 proteins were found to have only supportive roles during infection of the target cell by FIPV (<xref rid="b0710" ref-type="bibr">Dedeurwaerder et al., 2013</xref>). It has also been shown that the FIPV p7a protein is a type-I IFN antagonist but needs the presence of ORF3 proteins to fully exert its antagonistic function (<xref rid="b0075" ref-type="bibr">Dedeurwaerder et al., 2014</xref>).</p><p id="p0225">The ORF3 and ORF7 proteins of Porcine Respiratory coronavirus (PRCoV) and Transmissible Gastroenteritis Virus (TGEV) are known to play potential roles in determining virulence (<xref rid="b0805" ref-type="bibr">Paul et al., 1997</xref>, <xref rid="b0720" ref-type="bibr">McGoldrick et al., 1999</xref>, <xref rid="b0725" ref-type="bibr">Tung et al., 1992</xref>).</p><p id="p0230">Another notable accessory protein of an alphacoronavirus is the protein encoded by ORF4 in Human Coronavirus 229E (HCoV 229E; <xref rid="f0025" ref-type="fig">Fig. 5</xref>). It has been previously shown that the HCoV-229E genome codes for two accessory proteins, p4a and p4b. However, recent sequencing of clinical isolates revealed the presence of a full-length p4, whereas laboratory strains show the presence of a truncated p4 (<xref rid="b0730" ref-type="bibr">Farsani et al., 2012</xref>). It has been suggested that extensive culturing of HCoV 229E might have resulted in this truncation (<xref rid="b0735" ref-type="bibr">Dijkman et al., 2006</xref>). The high degree of conservation of the amino-acid sequence of p4 among clinical isolates suggests that the protein plays an important role <italic>in vivo</italic>.</p></sec><sec id="s0060"><label>5</label><title>Accessory proteins in <italic>Gammacoronavirus</italic></title><p id="p0240">The most studied gammacoronavirus to date is IBV. The genomic organization of a classic gammacoronavirus is 5′-UTR-Pol-S-3a-3b-E-M-5a-5b-N-UTR-3′ (UTR = untranslated region). Hence, it contains four accessory genes. The IBV sub-genomic mRNA 3 (sgRNA3) and sgRNA5 are known to be polycistronic (<xref rid="b0740" ref-type="bibr">Liu et al., 1991</xref>, <xref rid="b0745" ref-type="bibr">Liu and Inglis, 1992a</xref>, <xref rid="b0755" ref-type="bibr">Liu and Inglis, 1992b</xref>). The sgRNA3 has been shown to be tricistronic and encodes 3a, 3b and E protein (previously known as 3c protein) (<xref rid="b0740" ref-type="bibr">Liu et al., 1991</xref>, <xref rid="b0750" ref-type="bibr">Liu and Inglis, 1991</xref>, <xref rid="b0745" ref-type="bibr">Liu and Inglis, 1992a</xref>, <xref rid="b0755" ref-type="bibr">Liu and Inglis, 1992b</xref>), whereas sgRNA5 is bi-cistronic and encodes 5a and 5b (<xref rid="b0745" ref-type="bibr">Liu and Inglis, 1992a</xref>, <xref rid="b0755" ref-type="bibr">Liu and Inglis, 1992b</xref>). Serial passages of IBV in Vero cells led to the emergence of a mutant IBV with a truncated 3b gene (<xref rid="b0050" ref-type="bibr">Shen et al., 2003</xref>). The mutant virus is more virulent in chicken embryos, suggesting that the 3b protein may play a certain function in viral pathogenesis (<xref rid="b0050" ref-type="bibr">Shen et al., 2003</xref>). Reverse genetics studies have shown that proteins 3a, 3b. 5a, and 5b are dispensable for viral replication (<xref rid="b0055" ref-type="bibr">Hodgson et al., 2006</xref>, <xref rid="b0060" ref-type="bibr">Casais et al., 2005</xref>). In non-classical Australian strains of IBV, accessory genes 3a and 3b were found to be replaced by the unrelated X1 gene, and 5a was lacking (<xref rid="b0760" ref-type="bibr">Mardani et al., 2008</xref>). More recently, it has been demonstrated that a potential ORF situated between the M gene and gene 5a/b of IBV is transcribed through template-switching involving a non-canonical TRS, resulting in a 11-kD-protein of unknown function (<xref rid="b0765" ref-type="bibr">Bentley et al., 2013</xref>).</p></sec><sec id="s0065"><label>6</label><title>Conclusions</title><p id="p0245">SARS-CoV encodes eight accessory proteins, namely p3a, p3b, p6, p7a, p7b, p8a, p8b, and p9b. Reverse genetic studies have demonstrated that, whereas none of these proteins is essential for SARS-CoV replication, some of them, in particular p6 and p7b, may contribute to the virulence of SARS-CoV (<xref rid="b0360" ref-type="bibr">Pewe et al., 2005</xref>, <xref rid="b0520" ref-type="bibr">Pfefferle et al., 2009</xref>). Five of the SARS-CoV accessory proteins (p3a, p6, p7a, p7b, and p9b) have been shown to be incorporated into mature virions and are thus minor structural proteins. Most SARS-CoV accessory proteins are involved in more than one cellular process, such as interfering with the MAP-kinase pathway, DNA synthesis, induction of pro-inflammatory cytokines, or induction of caspase-dependent apoptosis, to name just a few. The crystal structures of SARS-CoV accessory proteins 7a and 9b have been determined, providing a structural basis for their biological functions (<xref rid="b0440" ref-type="bibr">Nelson et al., 2005</xref>, <xref rid="b0590" ref-type="bibr">Meier et al., 2006</xref>). However, the pathophysiological significances of these accessory proteins in the context of SARS-CoV infection are not completely understood, and further structural and functional studies are required.</p><p id="p0250">Knowledge on the accessory proteins of other coronaviruses is even more limited than for SARS-CoV. The best characterized member of betacoronaviruses of lineage a, murine coronavirus (MHV), as well as some related viruses encode a hemagglutinin esterase and a 2’,5’-oligoadenylate phosphodiesterase, the latter of which is involved in reducing the activities of RNase L. Lineage-a betacoronaviruses also encode an “internal” protein through a gene completely overlapping with the nucleocapsid gene. Betacoronaviruses of lineage c, the most prominent example for which is the newly emerging Middle East Respiratory Syndrome coronavirus (MERS-CoV), feature a similar gene “within” the nucleocapsid gene. The bat coronavirus HKU9, a member of betacoronavirus lineage d, and some alphacoronaviruses such as Feline Infectious Peritonitis Virus (FIPV) contain open reading frames downstream of the nucleocapsid gene. Proteins 7a and 7b of FIPV are involved in counteracting the antiviral response exerted by type-I interferons, with support from proteins 3a and 3b. 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