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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The coronavirus nucleocapsid protein is ADP-ribosylated</title>
<author><name sortKey="Grunewald, Matthew E" sort="Grunewald, Matthew E" uniqKey="Grunewald M" first="Matthew E." last="Grunewald">Matthew E. Grunewald</name>
</author>
<author><name sortKey="Fehr, Anthony R" sort="Fehr, Anthony R" uniqKey="Fehr A" first="Anthony R." last="Fehr">Anthony R. Fehr</name>
</author>
<author><name sortKey="Athmer, Jeremiah" sort="Athmer, Jeremiah" uniqKey="Athmer J" first="Jeremiah" last="Athmer">Jeremiah Athmer</name>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">29199039</idno>
<idno type="pmc">5871557</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871557</idno>
<idno type="RBID">PMC:5871557</idno>
<idno type="doi">10.1016/j.virol.2017.11.020</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">001578</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001578</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The coronavirus nucleocapsid protein is ADP-ribosylated</title>
<author><name sortKey="Grunewald, Matthew E" sort="Grunewald, Matthew E" uniqKey="Grunewald M" first="Matthew E." last="Grunewald">Matthew E. Grunewald</name>
</author>
<author><name sortKey="Fehr, Anthony R" sort="Fehr, Anthony R" uniqKey="Fehr A" first="Anthony R." last="Fehr">Anthony R. Fehr</name>
</author>
<author><name sortKey="Athmer, Jeremiah" sort="Athmer, Jeremiah" uniqKey="Athmer J" first="Jeremiah" last="Athmer">Jeremiah Athmer</name>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
</author>
</analytic>
<series><title level="j">Virology</title>
<idno type="ISSN">0042-6822</idno>
<idno type="eISSN">1096-0341</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated. ADP-ribosylation of N protein was also observed in cells exogenously expressing N protein by transduction using Venezuelan equine encephalitis virus replicon particles (VRPs). However, plasmid-derived N protein was not ADP-ribosylated following transient transfection but was ADP-ribosylated after MHV infection, indicating that this modification requires virus infection. In conclusion, we have identified a novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virology</journal-id>
<journal-id journal-id-type="iso-abbrev">Virology</journal-id>
<journal-title-group><journal-title>Virology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0042-6822</issn>
<issn pub-type="epub">1096-0341</issn>
<publisher><publisher-name>Elsevier Inc.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">29199039</article-id>
<article-id pub-id-type="pmc">5871557</article-id>
<article-id pub-id-type="publisher-id">S0042-6822(17)30399-9</article-id>
<article-id pub-id-type="doi">10.1016/j.virol.2017.11.020</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>The coronavirus nucleocapsid protein is ADP-ribosylated</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="au0005"><name><surname>Grunewald</surname>
<given-names>Matthew E.</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au0010"><name><surname>Fehr</surname>
<given-names>Anthony R.</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au0015"><name><surname>Athmer</surname>
<given-names>Jeremiah</given-names>
</name>
</contrib>
<contrib contrib-type="author" id="au0020"><name><surname>Perlman</surname>
<given-names>Stanley</given-names>
</name>
<email>Stanley-Perlman@uiowa.edu</email>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff id="aff0005">Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA 52242, United States</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author. <email>Stanley-Perlman@uiowa.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>1</day>
<month>12</month>
<year>2017</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>4</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub"><day>1</day>
<month>12</month>
<year>2017</year>
</pub-date>
<volume>517</volume>
<fpage>62</fpage>
<lpage>68</lpage>
<history><date date-type="received"><day>1</day>
<month>10</month>
<year>2017</year>
</date>
<date date-type="rev-recd"><day>20</day>
<month>11</month>
<year>2017</year>
</date>
<date date-type="accepted"><day>23</day>
<month>11</month>
<year>2017</year>
</date>
</history>
<permissions><copyright-statement>© 2017 Elsevier Inc.</copyright-statement>
<copyright-year>2017</copyright-year>
<copyright-holder>Elsevier Inc.</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract id="ab0010"><p>ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated. ADP-ribosylation of N protein was also observed in cells exogenously expressing N protein by transduction using Venezuelan equine encephalitis virus replicon particles (VRPs). However, plasmid-derived N protein was not ADP-ribosylated following transient transfection but was ADP-ribosylated after MHV infection, indicating that this modification requires virus infection. In conclusion, we have identified a novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein.</p>
</abstract>
<abstract abstract-type="author-highlights" id="ab0015"><title>Highlights</title>
<p><list list-type="simple" id="li0005"><list-item id="u0005"><label>•</label>
<p id="p0005">Mouse hepatitis virus nucleocapsid protein is ADP-ribosylated in virions and cells.</p>
</list-item>
<list-item id="u0010"><label>•</label>
<p id="p0010">ADP-ribosylation of N protein occurs during infection but not after transfection.</p>
</list-item>
<list-item id="u0015"><label>•</label>
<p id="p0015">ADP-ribosylation of N protein is conserved across many lineages of coronaviruses.</p>
</list-item>
</list>
</p>
</abstract>
<kwd-group id="keys0005"><title>Keywords</title>
<kwd>Coronavirus</kwd>
<kwd>Mouse hepatitis virus</kwd>
<kwd>Nucleocapsid</kwd>
<kwd>ADP-ribosylation</kwd>
<kwd>Macrodomain</kwd>
<kwd>PEDV</kwd>
<kwd>MERS-CoV</kwd>
<kwd>SARS-CoV</kwd>
</kwd-group>
</article-meta>
</front>
<body><sec id="s0005"><label>1</label>
<title>Introduction</title>
<p id="p0020">ADP-ribosylation is the covalent attachment of ADP-ribose (ADPr) moieties to a protein, resulting in either mono-ADPr (MAR) or poly-ADPr (PAR). ADP-ribosylation is catalyzed by poly-ADPr polymerases (PARPs), also known as ADP-ribosyltransferases (ARTDs). The PARP family consists of 17 proteins in humans and 16 in mice, which utilize NAD as the ADPr donor [reviewed in (<xref rid="bib6" ref-type="bibr">Bock and Chang, 2016</xref>
)]. Removal of ADPr from proteins (de-ADP-ribosylation) is catalyzed by different cellular proteins including PAR glycohydrolase (PARG) and macrodomain proteins (<xref rid="bib4" ref-type="bibr">Bernardi et al., 1997</xref>
, <xref rid="bib24" ref-type="bibr">Jankevicius et al., 2013</xref>
, <xref rid="bib40" ref-type="bibr">Rosenthal et al., 2013</xref>
, <xref rid="bib41" ref-type="bibr">Sharifi et al., 2013</xref>
). Detection of ADP-ribosylated proteins on a proteomic level is difficult due to the reactive nature and short half-life of the modification in cells (<xref rid="bib7" ref-type="bibr">Cervantes-Laurean et al., 1997</xref>
, <xref rid="bib45" ref-type="bibr">Wielckens et al., 1982</xref>
). Despite this, studies of individual PARPs and ADP-ribosylated proteins have elucidated several physiological roles for ADP-ribosylation, including DNA damage and repair, regulation of RNA transcription, cellular stress response, inflammation, differentiation, and apoptosis (<xref rid="bib6" ref-type="bibr">Bock and Chang, 2016</xref>
).</p>
<p id="p0025">PARPs are well-established to have both proviral and antiviral properties. PARP1 has been shown to facilitate Epstein-Barr virus replication and latency, simian virus 40 induction of cellular necrosis, and HIV integration (<xref rid="bib17" ref-type="bibr">Gordon-Shaag et al., 2003</xref>
, <xref rid="bib20" ref-type="bibr">Ha et al., 2001</xref>
, <xref rid="bib33" ref-type="bibr">Lupey-Green et al., 2017</xref>
, <xref rid="bib43" ref-type="bibr">Tempera et al., 2010</xref>
). TiPARP has been shown to inhibit interferon (IFN) production by ADP-ribosylation of TBK-1, leading to enhanced replication of several viruses (<xref rid="bib47" ref-type="bibr">Yamada et al., 2016</xref>
). Finally, ADP-ribosylation of the adenovirus core protein has been implicated in aiding viral replication and modulating stability of viral chromatin-like structures (<xref rid="bib10" ref-type="bibr">Dery et al., 1986</xref>
). Other data suggest that PARPs can also be antiviral. Many PARPs are expressed following IFN stimulation and several PARPs show evidence of rapid evolution, suggesting a microbial “arms race” between PARPs and cellular pathogens (<xref rid="bib1" ref-type="bibr">Atasheva et al., 2012</xref>
, <xref rid="bib9" ref-type="bibr">Daugherty et al., 2014</xref>
, <xref rid="bib34" ref-type="bibr">MacDonald et al., 2007</xref>
). For example, PARP7, PARP10, and PARP12 restrict Venezuelan equine encephalitis virus (VEEV) replication and can block cellular translation when overexpressed (<xref rid="bib1" ref-type="bibr">Atasheva et al., 2012</xref>
, <xref rid="bib2" ref-type="bibr">Atasheva et al., 2014</xref>
). One notable PARP, the Zinc-finger antiviral protein (ZAP) or PARP13, has been demonstrated to inhibit replication of several different viruses, potentially by binding to viral RNA and directing it to be degraded by the RNA exosome (<xref rid="bib5" ref-type="bibr">Bick et al., 2003</xref>
, <xref rid="bib15" ref-type="bibr">Gao et al., 2002</xref>
, <xref rid="bib18" ref-type="bibr">Guo et al., 2004</xref>
, <xref rid="bib19" ref-type="bibr">Guo et al., 2007</xref>
, <xref rid="bib32" ref-type="bibr">Liu et al., 2015</xref>
, <xref rid="bib36" ref-type="bibr">Muller et al., 2007</xref>
, <xref rid="bib52" ref-type="bibr">Zhu et al., 2011</xref>
). PARP13 is enzymatically inactive, and thus its antiviral activity is independent of ADP-ribosylation. In addition, Liu et al. have described a novel mechanism in which an unknown PARP ADP-ribosylates two subunits of the influenza RNA polymerase, allowing subsequent binding to ZAP and degradation of these subunits by the proteasome (<xref rid="bib32" ref-type="bibr">Liu et al., 2015</xref>
).</p>
<p id="p0030">Three different virus families, Hepeviridae, Togaviridae, and Coronaviridae, encode for a viral macrodomain, which has been shown to de-ADP-ribosylate proteins <italic>in vitro</italic>
(<xref rid="bib31" ref-type="bibr">Li et al., 2016</xref>
, <xref rid="bib40" ref-type="bibr">Rosenthal et al., 2013</xref>
). These macrodomains have been proposed to counter the antiviral effects of ADP-ribosylation during infection. Our previous work has focused on the coronavirus (CoVs) macrodomain. CoVs are large, positive-sense, single-stranded RNA viruses which include human pathogens such as the severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV as well as important veterinary pathogens such as bovine CoV and porcine epidemic disease virus (PEDV). All CoVs encode a macrodomain within non-structural protein 3 (nsp3) that can remove both MAR and PAR from proteins (<xref rid="bib31" ref-type="bibr">Li et al., 2016</xref>
). CoVs lacking this enzymatic activity generally replicate normally <italic>in vitro</italic>
but are highly attenuated <italic>in vivo</italic>
and elicit an enhanced innate immune response (<xref rid="bib12" ref-type="bibr">Eriksson et al., 2008</xref>
, <xref rid="bib13" ref-type="bibr">Fehr et al., 2015</xref>
, <xref rid="bib14" ref-type="bibr">Fehr et al., 2016</xref>
, <xref rid="bib29" ref-type="bibr">Kuri et al., 2011</xref>
).</p>
<p id="p0035">To identify potential targets of the CoV macrodomain, we analyzed infected cells for changes in ADP-ribosylation patterns utilizing antibodies specific for ADPr. We focused on cells infected with a murine CoV, mouse hepatitis virus (MHV). MHV causes acute and chronic encephalomyelitis, hepatitis and gastroenteritis (<xref rid="bib3" ref-type="bibr">Bailey et al., 1949</xref>
). Surprisingly, we found that the CoV nucleocapsid (N) protein was ADP-ribosylated in cells during infection with MHV as well as several other CoVs.</p>
</sec>
<sec id="s0010"><label>2</label>
<title>Materials and methods</title>
<sec id="s0015"><label>2.1</label>
<title>Cell culture, plasmids and reagents</title>
<p id="p0040">Delayed brain tumor (DBT) cells, 17Cl-1 cells, Vero cells, and HeLa cells expressing the MHV receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) (HeLa-MHVR) were grown in Dulbecco's modified Eagle medium with 10% fetal bovine serum as previously described (<xref rid="bib51" ref-type="bibr">Zhou and Perlman, 2007</xref>
). Codon-optimized MHV-A59 N protein was synthesized and cloned directly into pcDNA3 (GenScript). A tagged construct was synthesized by inserting a 3X-FLAG sequence to the C terminus of the N protein using overlapping primers and recombination by In-fusion (Clontech). Control plasmid pcDNA3-GFP was described previously (<xref rid="bib14" ref-type="bibr">Fehr et al., 2016</xref>
).</p>
</sec>
<sec id="s0020"><label>2.2</label>
<title>Virus Infection</title>
<p id="p0045">Recombinant mouse hepatitis virus (MHV) strains A59 (<xref rid="bib48" ref-type="bibr">Yount et al., 2002</xref>
) and JHMV (wild-type and N1347A) (<xref rid="bib13" ref-type="bibr">Fehr et al., 2015</xref>
) were propagated on 17Cl-1 cells, and titers were determined on HeLa-MHVR cells. SARS-CoV (MA15) was propagated and titered on Vero E6 cells, and MERS-CoV (EMC12) and PEDV (ISU13-19338E, a gift from Dr. Kyoung-Jin Yoon, Iowa State University) were propagated on Vero-81 cells. For virus infections, 17Cl-1, DBT, Calu-3, Vero E6, or Vero 81 cells were infected with virus at the indicated multiplicity of infection (MOI) and collected at the indicated hours post-infection (hpi). All work with SARS-CoV or MERS-CoV infectious virus was performed in a biosafety level 3 laboratory according to the guidelines set forth by the University of Iowa.</p>
</sec>
<sec id="s0025"><label>2.3</label>
<title>Proteinase K treatment of virions</title>
<p id="p0050">DBT cells were infected with MHV-A59 at an MOI of 0.5 PFU/cell, and supernatant was collected and filtered at 12 hpi. The filtrate was subjected to ultracentrifugation at 27,000 rpm for 4 h over a 30% sucrose cushion as described previously. Pellets were resuspended in 100 mM NaCl and 10 mM Tris-Cl (pH 7.2) and treated with or without Proteinase K (New England Biolabs) in the presence or absence of SDS. The reaction was stopped by incubation at 65 °C for 10 min.</p>
</sec>
<sec id="s0030"><label>2.4</label>
<title>Virus transfection and transduction</title>
<p id="p0055">Cells were transfected with PolyJet <italic>In Vitro</italic>
Transfection Reagent (SignaGen Labs) as per the manufacturer's instructions. 24 h after transfection, cells were either treated with or without 1000 U/ml of IFN-β (PBL) for 24 h or were infected with MHV-A59 at an MOI of 1 PFU/cell for 12 h before collection. VEEV replicon particles (VRPs) encoding either GFP or MERS nucleocapsid protein were created and titered as previously reported (<xref rid="bib49" ref-type="bibr">Zhao et al., 2014</xref>
, <xref rid="bib50" ref-type="bibr">Zhao et al., 2016</xref>
). The VRPs were transduced into Vero 81 cells at indicated MOIs and collected at 24 h post-transduction.</p>
</sec>
<sec id="s0035"><label>2.5</label>
<title>Immunoblotting</title>
<p id="p0060">Sample buffer containing SDS, β-mercaptoethanol, protease/phosphatase inhibitor cocktails (Roche), PMSF, PARP inhibitor 3-aminobenzamide (3-AB, Tocris Bioscience), PARG inhibitor adenosine 5ʹ-diphosphate (hydroxymethyl)pyrrolidinediol (ADP-HPD, Calbiochem) and universal nuclease (ThermoFisher Scientific) was used to collect cell lysates. Proteins were resolved on an SDS polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane. Following binding with a primary antibody, blots were then visualized by using a peroxidase-conjugated secondary antibody (Thermo Fisher Scientific) detected with a chemiluminescent substrate (Thermo Fisher Scientific) or by using an infrared (IR) dye-conjugated secondary antibody detected with a Li-COR Odyssey Imager (Li-COR, Lincoln, NE). IR secondary antibodies of different wavelengths were used to obtain different signals for antibody bound proteins. Images of α-ADPr- or α-N-stained immunoblots were merged using Image Studio software.</p>
<p id="p0065">Primary antibodies used for immunoblotting and immunoprecipitation included polyclonal (pAb) α-MHV rabbit serum (<xref rid="bib39" ref-type="bibr">Perlman et al., 1987</xref>
), monoclonal (mAb) α-MHV N (<xref rid="bib8" ref-type="bibr">Collins et al., 1982</xref>
) (mAb 5B188.2, a kind gift from Dr. M. Buchmeier, University of California, Irvine), pAb α-SARS-CoV N (Novus Biologicals), pAb α-nsp3 (gift from Mark Denison, Vanderbilt University), mAb α-PEDV N (gift from Dr. Kyoung-Jin Yoon, Iowa State University), pAb α-MERS-CoV N (<xref rid="bib50" ref-type="bibr">Zhao et al., 2016</xref>
), mouse mAb α-ADPr (10 H, Millipore Sigma), rabbit pAb α-ADPr (Trevigen), chicken pAb α-ADPr (Tulip BioLabs Inc.), α-FLAG (Sigma), α-GAPDH (Poly6314, BioLegend), and α-actin (AC15; Abcam, Inc.) antibodies. Secondary antibodies used included horseradish peroxidase-conjugated α-rabbit or α-mouse (Sigma #A0545/A0168) antibodies or IR-conjugated α-rabbit, α-mouse, or α-chicken (Li-COR, #926–68071/926–32210/925–32218) antibodies.</p>
</sec>
<sec id="s0040"><label>2.6</label>
<title>Immunoprecipitation</title>
<p id="p0070">DBT cells infected with MHV-A59 at an MOI of 1 PFU/cell were collected at 12 hpi and pelleted by low-speed centrifugation. Cell pellets were lysed with immunoprecipitation (IP) buffer (0.5% NP-40, 300 mM NaCl, 5% glycerol, and 50 mM Tris pH 8.0) containing protease/phosphatase inhibitor cocktails, PMSF, PARP inhibitor 3-AB, PARG inhibitor ADP-HPD, and a universal nuclease for 2 h at 4 °C. Nuclei were pelleted by centrifugation (16,000 g for 15 min at 4 °C). One aliquot of cell lysate was saved as the input control and boiled in SDS sample buffer described above. Protein G magnetic beads were conjugated to α-ADPr or α-N antibodies (described above) as per manufacturer's instructions (ThermoFisher Scientific). Protein G antibody-conjugated were mixed with cell lysates overnight at 4 °C. Beads were washed with PBS-Tween before elution by boiling in SDS sample buffer.</p>
</sec>
</sec>
<sec id="s0045"><label>3</label>
<title>Results</title>
<sec id="s0050"><label>3.1</label>
<title>The MHV nucleocapsid protein is ADP-ribosylated in cell culture</title>
<p id="p0075">To screen for changes in protein ADP-ribosylation during CoV infection, we infected DBT cells, an astrocytoma cell line, with the A59 strain of MHV. Cells were collected throughout the infection, and cell lysates were immunoblotted with a mouse mAb antibody to ADPr (mAb 10 H). The 10 H antibody has been described to bind preferentially to linear 20+-mers of PAR with no binding activity to DNA, RNA, or adenosine-monophosphate. However, more recent reports have demonstrated that mAb 10 H also binds to auto-MARylated proteins (<xref rid="bib11" ref-type="bibr">Eckei et al., 2017</xref>
, <xref rid="bib16" ref-type="bibr">Goenka et al., 2007</xref>
, <xref rid="bib25" ref-type="bibr">Kawamitsu et al., 1984</xref>
, <xref rid="bib26" ref-type="bibr">Kleine et al., 2008</xref>
). While the ADP-ribosylation status of most proteins did not change over the course of infection, we noted the appearance of a ~55 kDa band at 8 h post-infection (hpi) which increased in abundance up to 12 hpi (
<xref rid="f0005" ref-type="fig">Fig. 1</xref>
A). Immunoblotting with two other commercial α-ADPr antibodies raised in two other species also detected a similar protein band (<xref rid="f0005" ref-type="fig">Fig. 1</xref>
A). A negative control antibody (α-HA) did not bind to this protein, suggesting a specific interaction of the 55 kDa protein with ADPr antibodies. Based on to the size and abundance of this protein, we hypothesized that it was the nucleocapsid (N) protein. Staining with monoclonal α-N antibody produced a signal that completely overlapped the signal from the ADPr antibody (<xref rid="f0005" ref-type="fig">Fig. 1</xref>
B, left). To confirm that this overlap was consistent in other cell lines and MHV strains, we infected 17Cl-1 cells (a fibroblast cell line) with MHV-A59 or DBT cells with MHV-JHM (JHMV), a neurotropic strain of MHV, and immunoblotted with α-MHV serum or α-ADPr antibody. The results showed that in all cases the N protein completely overlapped with the ~55 kDa ADP-ribosylated protein, demonstrating that N protein ADP-ribosylation is not MHV strain or cell type specific (<xref rid="f0005" ref-type="fig">Fig. 1</xref>
B, middle and right). To confirm the identity of this protein as the N protein, we collected MHV-infected DBT cells at 12 hpi and immunoprecipitated proteins with either monoclonal α-ADPr or α-N antibodies and immunoblotted with the reciprocal antibodies. As expected, this ~55 kDa protein could be stained with the α-ADPr antibody after immunoprecipitation with α-N and stained with α-N after immunoprecipitation with α-ADPr (<xref rid="f0005" ref-type="fig">Fig. 1</xref>
C), confirming that the N protein is ADP-ribosylated.<fig id="f0005"><label>Fig. 1</label>
<caption><p>The MHV nucleocapsid protein is ADP-ribosylated. (A) Expression of the N protein coincides with the appearance of a ADP-ribosylated band during MHV-A59 infection. DBT cells were mock infected (M) or infected with MHV-A59 at an MOI of 1 PFU/cell and collected at 4, 8, or 12 hpi. Proteins were analyzed by immunoblotting with indicated antibodies. The ~55 kDa N protein band is denoted by an arrow. (B) ADP-ribosylation of the N protein is conserved amongst different MHV strains and in multiple cell types. Indicated cells were mock infected (M) or infected with A59 or JHMV at an MOI of 1 or 0.1 PFU/cell, respectively. Lysates were collected at 12 and 18 hpi, respectively, and immunoblotted with indicated antibodies. (C) Immunoprecipitation with α-ADPr antibody confirms N protein is ADP-ribosylated. DBT cells were mock infected (M) or infected with MHV-A59 at MOI of 1 PFU/cell. At 12 hpi, cells were collected and the lysates were subjected to immunoprecipitation with mouse α-ADPr or α-N (mAb) bound to Protein G beads. Cell lysates and eluted proteins were analyzed by immunoblotting with indicated antibodies. Asterisk indicates cellular ADP-ribosylated protein that is not immunoprecipitated with α-N antibody.</p>
</caption>
<alt-text id="at0005">Fig. 1</alt-text>
<graphic xlink:href="gr1_lrg"></graphic>
</fig>
</p>
</sec>
<sec id="s0055"><label>3.2</label>
<title>The Nsp3 macrodomain does not de-ADP-ribosylate the N protein</title>
<p id="p0080">Previous reports have demonstrated that the nsp3 macrodomain removes both mono- and poly-ADPr from auto-ADP-ribosylated substrates <italic>in vitro</italic>
(<xref rid="bib14" ref-type="bibr">Fehr et al., 2016</xref>
, <xref rid="bib31" ref-type="bibr">Li et al., 2016</xref>
). Because the N protein is known to associate with nsp3, we speculated that the nsp3 macrodomain may de-ADP-ribosylate the N protein (<xref rid="bib22" ref-type="bibr">Hurst et al., 2013</xref>
). To test this, we infected DBT cells with MHV-A59 or MHV-JHM encoding either wild-type or a catalytically-deficient (JHMV-N1347A, A59-N1348A) nsp3 macrodomain and immunoblotted for N protein ADP-ribosylation. If the macrodomain was indeed removing the ADPr from the N protein, we would expect to see increased N protein ADP-ribosylation in cells infected with mutant virus compared to wild-type infected cells. However, we found that the level of the ADP-ribosylated N protein was the same in cells infected with either MHV strain (
<xref rid="f0010" ref-type="fig">Fig. 2</xref>
). These findings suggest that the nsp3 macrodomain does not affect N protein ADP-ribosylation under these conditions.<fig id="f0010"><label>Fig. 2</label>
<caption><p>The catalytic ability of nsp3 does not reverse N protein ADP-ribosylation. DBT cells were infected with MHV-A59 at MOI of 0.1 PFU/cell or MHV-JHM at MOI of 0.2 PFU/cell encoding a wild-type (WT) or catalytically-deficient macrodomain of nsp3. MHV-A59-infected cells were collected at 15 hpi, and MHV-JHM-infected cells were collected at 18 hpi. Lysates were immunoblotted as indicated.</p>
</caption>
<alt-text id="at0010">Fig. 2</alt-text>
<graphic xlink:href="gr2_lrg"></graphic>
</fig>
</p>
</sec>
<sec id="s0060"><label>3.3</label>
<title>The Nucleocapsid protein ADP-ribosylation is conserved in ⍺ and β-coronaviruses</title>
<p id="p0085">Next, we tested whether N protein ADP-ribosylation was conserved in CoV genera and lineages other than MHV. We infected Vero cells with PEDV (⍺-CoV), SARS-CoV (lineage B β-CoV), or MERS-CoV (lineage C β-CoV) and then collected the cells and analyzed whether the N proteins from these viruses were ADP-ribosylated (
<xref rid="f0015" ref-type="fig">Fig. 3</xref>
). In all cases, ADPr antibody staining overlapped with the N protein of each virus, demonstrating that this modification is conserved across multiple genera and lineages of CoVs.<fig id="f0015"><label>Fig. 3</label>
<caption><p>Nucleocapsid ADP-ribosylation is conserved in other CoV genera. Mock (M) or virus infection of cells included: (A) PEDV infection of Vero cells at 0.1 PFU/cell for 48 h, (B) SARS-CoV infection of Calu-3 cells at 0.1 PFU/cell for 24 h, or (C) MERS-CoV infection of Vero cells at 0.1 PFU/cell for 24 h. Cell lysates were immunoblotted with indicated antibodies.</p>
</caption>
<alt-text id="at0015">Fig. 3</alt-text>
<graphic xlink:href="gr3_lrg"></graphic>
</fig>
</p>
</sec>
<sec id="s0065"><label>3.4</label>
<title>ADP-ribosylation of N protein is retained the MHV virion</title>
<p id="p0090">It has previously been shown that the N protein is phosphorylated and that some phosphorylated residues depend on whether the protein is intracellular or virion-associated (<xref rid="bib44" ref-type="bibr">White et al., 2007</xref>
). Specifically, MHV N protein at amino acid S197 is phosphorylated in infected cells, but this modification is absent on N protein in virions (<xref rid="bib46" ref-type="bibr">Wu et al., 2014</xref>
). To determine if N protein ADP-ribosylation is maintained within the virion, we purified virions and analyzed whether N protein from the CoV virion was ADP-ribosylated by immunoblotting. To rule out the possibility of detecting of any residual N protein from non-virion sources, pelleted virions were treated with proteinase K with or without virion lysis by SDS. Both the N protein as well as the spike (S) protein were detectable with α-MHV serum in both cells and virions (
<xref rid="f0020" ref-type="fig">Fig. 4</xref>
). Although the S protein was not completely eliminated upon proteinase K treatment, the abundance of the S protein was reduced, consistent with the protein being exposed on the surface of the virus. A co-sedimented nonspecific band of ~70 kDa was also degraded by proteinase K treatment. In contrast, N protein, located in the interior of the virion, was protected from proteinase K treatment unless SDS was also added to lyse the viral envelope. Importantly, immunoblotting with α-ADPr antibody demonstrated that the N protein maintained the ADP-ribose modification in virions.<fig id="f0020"><label>Fig. 4</label>
<caption><p>The N protein is ADP-ribosylated within the MHV-A59 virion. Supernatant from DBT cells infected with MHV-A59 at MOI of 0.5 was collected at 12 h. Whole virions were purified by filtration and ultracentrifugation with a 30% sucrose cushion. Virions were then treated with Proteinase K to degrade extramembranous protein or Proteinase K in the presence of SDS to degrade all viral proteins. Proteinase K was then inactivated at 65 °C, and proteins were blotted with indicated antibodies. The nucleocapsid (N), spike (S), and a nonspecific (ns) proteins are indicated with arrows.</p>
</caption>
<alt-text id="at0020">Fig. 4</alt-text>
<graphic xlink:href="gr4_lrg"></graphic>
</fig>
</p>
</sec>
<sec id="s0070"><label>3.5</label>
<title>Plasmid-expressed N protein is only ADP-ribosylated during infection</title>
<p id="p0095">To determine if N protein expressed in the absence of CoV infection could be ADP-ribosylated in cell culture, we transduced Vero cells with VEEV replicon particles (VRPs) encoding the MERS-CoV N protein or control GFP at different MOIs (<xref rid="bib50" ref-type="bibr">Zhao et al., 2016</xref>
). Transduced cells were collected at 24 hpi, and immunoblotting of cell lysates showed that N protein expressed from an alphavirus replicon was ADP-ribosylated (
<xref rid="f0025" ref-type="fig">Fig. 5</xref>
A).<fig id="f0025"><label>Fig. 5</label>
<caption><p>The N protein is only ADP-ribosylated in the presence of viral infection. (A) ADP-ribosylation of N protein is conserved in transduced cells. Vero cells were transduced with Venezuelan equine encephalitis virus replicon particles (VRPs) encoding green fluorescent protein (GFP) or MERS N protein at MOIs of 1, 5, or 10 PFU/cell. Cell lysates were blotted with indicated antibodies. (B) Transfected N protein is not ADP-ribosylated. DBT cells were transfected with plasmid encoding control GFP or codon-optimized MHV N protein for 24 h. Cells were then treated with or without 1000 U/ml of IFN-β for 24 h and collected. Cell lysate was immunoblotted with indicated antibodies. A positive control of MHV-A59-infected DBT cell lysate is also shown. (C) Transfected N protein is ADP-ribosylated after concurrent MHV infection. DBT cells were transfected with plasmid encoding control GFP or codon-optimized MHV N-FLAG protein for 24 h. Cells were then mock infected or infected with MHV-A59 at an MOI of 1 PFU/cell and collected at 12 hpi. Lysates were analyzed by immunoblotting with the indicated antibodies. N-FLAG is indicated by arrows.</p>
</caption>
<alt-text id="at0025">Fig. 5</alt-text>
<graphic xlink:href="gr5_lrg"></graphic>
</fig>
</p>
<p id="p0100">Because the VRP platform utilizes a virus infection to express exogenous proteins, we hypothesized that the ADP-ribosylation of the N protein may require a virus infection. To examine this possibility, we transfected DBT cells with a plasmid encoding codon-optimized MHV-N protein or GFP and then tested whether the N protein was ADP-ribosylated. Because several PARPs are IFN-stimulated genes, we also treated cells with or without IFN-β (<xref rid="bib1" ref-type="bibr">Atasheva et al., 2012</xref>
, <xref rid="bib34" ref-type="bibr">MacDonald et al., 2007</xref>
). Importantly, we were unable to detect any ADP-ribosylation of the exogenous transfected N protein (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
B). In contrast, a positive control of MHV-A59-infected cell lysate, normalized to total N protein, stained with α-ADPr antibody. Furthermore, IFN-β treatment did not rescue the modification, suggesting that other factors present during infection drove ADP-ribosylation of N protein. To confirm that N protein requires an infection to be ADP-ribosylated, we added a 3X-FLAG tag to our plasmid-expressed N (N-FLAG) protein. We then transfected plasmid expressing GFP or N-FLAG into DBT cells and then mock infected or infected cells with MHV-A59. At 12 hpi, we collected cells and immunoblotted with α-ADPr antibody. The N-FLAG protein was detectable with α-ADPr antibody following infection with MHV-A59 but not in mock infected cells (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
C). This suggests that N protein is only ADP-ribosylated within the context of virus infection.</p>
</sec>
</sec>
<sec id="s0075"><label>4</label>
<title>Discussion</title>
<p id="p0105">The N protein was initially identified as a major structural protein, binding directly to viral RNA, providing stability to the bound RNA, and self-oligomerizing into the virus nucleocapsid. N protein also binds to the viral membrane (M) protein to facilitate genome loading and viral assembly (<xref rid="bib37" ref-type="bibr">Narayanan et al., 2000</xref>
). It plays a prominent role in transcription and replication of the viral genome (<xref rid="bib23" ref-type="bibr">Hurst et al., 2010</xref>
). In fact, the addition of N protein from an exogenous plasmid is often utilized to initiate cellular infection from recombinant CoV cDNA (<xref rid="bib48" ref-type="bibr">Yount et al., 2002</xref>
). The N protein provides additional accessory functions, including the ability to promote cell cycle arrest, inhibit host translation, and block the IFN response during infection [reviewed in (<xref rid="bib35" ref-type="bibr">McBride et al., 2014</xref>
)]. Many of these functions are regulated by post-translational modifications, most notably phosphorylation by host proteins. For example, SARS-CoV N protein phosphorylation modulates N protein oligomerization, translation suppression, and localization to stress granules (<xref rid="bib38" ref-type="bibr">Peng et al., 2008</xref>
). Furthermore, phosphorylation of the MHV N protein is implicated to function as a cellular switch to control transcription of either genomic or subgenomic RNA (<xref rid="bib46" ref-type="bibr">Wu et al., 2014</xref>
). In this paper, we have identified an additional post-translational modification of the N protein, ADP-ribosylation, which could also play a role in the regulation of N protein functions.</p>
<p id="p0110">We have found that the N protein of multiple CoVs was APD-ribosylated <italic>in vitro,</italic>
using multiple antibodies to ADPr (<xref rid="f0005" ref-type="fig">Figs. 1</xref>
and <xref rid="f0015" ref-type="fig">3</xref>
). The 10 H α-ADPr antibody primarily used in this study binds preferentially to PAR but can be used to detect MARylated proteins as well (<xref rid="bib11" ref-type="bibr">Eckei et al., 2017</xref>
, <xref rid="bib16" ref-type="bibr">Goenka et al., 2007</xref>
, <xref rid="bib25" ref-type="bibr">Kawamitsu et al., 1984</xref>
, <xref rid="bib26" ref-type="bibr">Kleine et al., 2008</xref>
). Because the N protein band stained with either α-ADPr or α-N antibodies did not appear as a smear, which is seen with long-chain PARylated proteins, it is likely that the N protein is either MARylated or PARylated with only a few monomers of ADPr. This is further supported by the fact that ADP-ribosylation did not alter migration of the N protein to a detectable level (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
B and C). Furthermore, our data indicate that the N protein ADP-ribosylation was detectable during infection (<xref rid="f0005" ref-type="fig">Fig. 1</xref>
, <xref rid="f0025" ref-type="fig">Fig. 5</xref>
A, <xref rid="f0025" ref-type="fig">Fig. 5</xref>
C) but not following transfection alone (<xref rid="f0025" ref-type="fig">Fig. 5</xref>
B). This could be due to a number of factors including, but not limited to, virus infection-dependent expression of an ADP-ribosylating enzyme or the localization of the N protein to a distinct cellular compartment during infection. N protein in infected cells has been shown to localize both in the cytoplasm as well as the nucleus, and the SARS N protein has been shown to localize to stress granules under stress conditions (<xref rid="bib21" ref-type="bibr">Hiscox et al., 2001</xref>
, <xref rid="bib38" ref-type="bibr">Peng et al., 2008</xref>
). Several PARPs are known to co-localize with stress granules, which could potential sites of N protein modification (<xref rid="bib30" ref-type="bibr">Leung et al., 2011</xref>
). Future experiments are required to parse out the number of ADPr monomers that are attached to the N protein, to identify the amino acid residue(s) that is modified, and to determine where this modification occurs.</p>
<p id="p0115">The fact that ADP-ribosylation of N protein was conserved across multiple CoV lineages (<xref rid="f0015" ref-type="fig">Fig. 3</xref>
) suggests that it is important either for viral replication or pathogenesis or as a host defense mechanism. Individual PARPs have been identified to be either proviral or antiviral [reviewed in (<xref rid="bib28" ref-type="bibr">Kuny and Sullivan, 2016</xref>
)]. Because the N protein is the major structural protein of the CoV nucleocapsid, it is tempting to speculate that ADP-ribosylation is providing a regulatory role for the structure of the genome, similar to ADP-ribosylation of histones or of the adenovirus core protein (<xref rid="bib10" ref-type="bibr">Dery et al., 1986</xref>
, <xref rid="bib42" ref-type="bibr">Strickfaden et al., 2016</xref>
). It is also possible that ADP-ribosylation of N protein could regulate one or more of the other functions of the N protein such as inhibition of cellular translation or of IFN expression (<xref rid="bib27" ref-type="bibr">Kopecky-Bromberg et al., 2007</xref>
). On the other hand, ADP-ribosylation of the N protein may be an antiviral defense mechanism, similar to ADP-ribosylation of influenza A virus polymerase subunits (<xref rid="bib32" ref-type="bibr">Liu et al., 2015</xref>
). If this were the case, the virus must have evolved methods to combat this modification, possibly including removal of the ADP-ribose moieties from the N protein to mitigate its antiviral effects. However, we found that the nsp3 macrodomain, a likely candidate to catalyze this de-ADP-ribosylation, unexpectedly does not de-ADP-ribosylate the N protein based on our assays (<xref rid="f0010" ref-type="fig">Fig. 2</xref>
) (<xref rid="bib14" ref-type="bibr">Fehr et al., 2016</xref>
). However it is possible that a small, localized subset of the very abundant N protein is de-ADP-ribosylated, which would not be detectable in our assay. Future experiments will focus on identifying the function of N protein ADP-ribosylation and the specific targets of the CoV macrodomain.</p>
</sec>
<sec id="s0080"><label>5</label>
<title>Conclusions</title>
<p id="p0120">Here we have showed that the N proteins of multiple CoVs are ADP-ribosylated, representing a previously undescribed modification of a RNA virus structural protein. This modification only occurred during virus infection and was maintained both in the cell and in virions. Future experiments will work to identify the amino acids that are ADP-ribosylated and to demonstrate the effect of this novel modification on virus infection.</p>
</sec>
</body>
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<ack id="ack0005"><title>Acknowledgments</title>
<p>We thank the members of the Perlman, Wendy Maury, and Pat Sinn laboratories for valuable discussion, Kyoung-Jin Yoon for PEDV virus and antibodies, and Rudragouda Channappanavar for critical reading of the manuscript.</p>
<p>This study was supported by <funding-source id="gs2">National Institute of Health</funding-source>
grants to S.P. (P01 AI060699 and R01 NS36592). M.G. and J.A. were supported by institutional predoctoral <funding-source id="gs1">NRSA</funding-source>
training grants (T32 AI007511 and T32 AI007533, respectively). A.R.F was supported by institutional (T32-AI007260) and individual (F32-AI113973) <funding-source id="gs3">NIH NRSA</funding-source>
grants.</p>
</ack>
<ack id="ack0010"><sec id="s0085"><title>Conflicts of interest</title>
<p id="p0135">None.</p>
</sec>
</ack>
</back>
</pmc>
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