Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Targeting N-Glycan Cryptic Sugar Moieties for Broad-Spectrum Virus Neutralization: Progress in Identifying Conserved Molecular Targets in Viruses of Distinct Phylogenetic Origins</title><author><name sortKey="Wang, Denong" sort="Wang, Denong" uniqKey="Wang D" first="Denong" last="Wang">Denong Wang</name><affiliation><nlm:aff id="af1-molecules-20-04610">Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail:<email>jiafengjushi@live.com</email></nlm:aff></affiliation></author><author><name sortKey="Tang, Jin" sort="Tang, Jin" uniqKey="Tang J" first="Jin" last="Tang">Jin Tang</name><affiliation><nlm:aff id="af1-molecules-20-04610">Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail:<email>jiafengjushi@live.com</email></nlm:aff></affiliation></author><author><name sortKey="Tang, Jiulai" sort="Tang, Jiulai" uniqKey="Tang J" first="Jiulai" last="Tang">Jiulai Tang</name><affiliation><nlm:aff id="af2-molecules-20-04610">Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China; E-Mail:<email>tangjiulai8888@21cn.com</email></nlm:aff></affiliation></author><author><name sortKey="Wang, Lai Xi" sort="Wang, Lai Xi" uniqKey="Wang L" first="Lai-Xi" last="Wang">Lai-Xi Wang</name><affiliation><nlm:aff id="af3-molecules-20-04610">Institute of Human Virology, Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; E-Mail:<email>LWang@ihv.umaryland.edu</email></nlm:aff></affiliation><affiliation><nlm:aff id="af4-molecules-20-04610">Department of Chemistry and Biochemistry, University of Maryland at College Park, College Park, MD 20742, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25774492</idno><idno type="pmc">4633014</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633014</idno><idno type="RBID">PMC:4633014</idno><idno type="doi">10.3390/molecules20034610</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">001300</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001300</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Targeting N-Glycan Cryptic Sugar Moieties for Broad-Spectrum Virus Neutralization: Progress in Identifying Conserved Molecular Targets in Viruses of Distinct Phylogenetic Origins</title><author><name sortKey="Wang, Denong" sort="Wang, Denong" uniqKey="Wang D" first="Denong" last="Wang">Denong Wang</name><affiliation><nlm:aff id="af1-molecules-20-04610">Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail:<email>jiafengjushi@live.com</email></nlm:aff></affiliation></author><author><name sortKey="Tang, Jin" sort="Tang, Jin" uniqKey="Tang J" first="Jin" last="Tang">Jin Tang</name><affiliation><nlm:aff id="af1-molecules-20-04610">Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail:<email>jiafengjushi@live.com</email></nlm:aff></affiliation></author><author><name sortKey="Tang, Jiulai" sort="Tang, Jiulai" uniqKey="Tang J" first="Jiulai" last="Tang">Jiulai Tang</name><affiliation><nlm:aff id="af2-molecules-20-04610">Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China; E-Mail:<email>tangjiulai8888@21cn.com</email></nlm:aff></affiliation></author><author><name sortKey="Wang, Lai Xi" sort="Wang, Lai Xi" uniqKey="Wang L" first="Lai-Xi" last="Wang">Lai-Xi Wang</name><affiliation><nlm:aff id="af3-molecules-20-04610">Institute of Human Virology, Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; E-Mail:<email>LWang@ihv.umaryland.edu</email></nlm:aff></affiliation><affiliation><nlm:aff id="af4-molecules-20-04610">Department of Chemistry and Biochemistry, University of Maryland at College Park, College Park, MD 20742, USA</nlm:aff></affiliation></author></analytic><series><title level="j">Molecules</title><idno type="eISSN">1420-3049</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Identifying molecular targets for eliciting broadly virus-neutralizing antibodies is one of the key steps toward development of vaccines against emerging viral pathogens. Owing to genomic and somatic diversities among viral species, identifying protein targets for broad-spectrum virus neutralization is highly challenging even for the same virus, such as HIV-1. However, viruses rely on host glycosylation machineries to synthesize and express glycans and, thereby, may display common carbohydrate moieties. Thus, exploring glycan-binding profiles of broad-spectrum virus-neutralizing agents may provide key information to uncover the carbohydrate-based virus-neutralizing epitopes. In this study, we characterized two broadly HIV-neutralizing agents, human monoclonal antibody 2G12 and Galanthus nivalis lectin (GNA), for their viral targeting activities. Although these agents were known to be specific for oligomannosyl antigens, they differ strikingly in virus-binding activities. The former is HIV-1 specific; the latter is broadly reactive and is able to neutralize viruses of distinct phylogenetic origins, such as HIV-1, severe acute respiratory syndrome coronavirus (SARS-CoV), and human cytomegalovirus (HCMV). In carbohydrate microarray analyses, we explored the molecular basis underlying the striking differences in the spectrum of anti-virus activities of the two probes. Unlike 2G12, which is strictly specific for the high-density Man<sub>9</sub>GlcNAc<sub>2</sub>Asn (Man9)-clusters, GNA recognizes a number of N-glycan cryptic sugar moieties. These include not only the known oligomannosyl antigens but also previously unrecognized tri-antennary or multi-valent GlcNAc-terminating N-glycan epitopes (Tri/m-Gn). These findings highlight the potential of N-glycan cryptic sugar moieties as conserved targets for broad-spectrum virus neutralization and suggest the GNA-model of glycan-binding warrants focused investigation.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Calarese, D A" uniqKey="Calarese D">D.A. Calarese</name></author><author><name sortKey="Scanlan, C N" uniqKey="Scanlan C">C.N. Scanlan</name></author><author><name sortKey="Zwick, M B" uniqKey="Zwick M">M.B. Zwick</name></author><author><name sortKey="Deechongkit, S" uniqKey="Deechongkit S">S. Deechongkit</name></author><author><name sortKey="Mimura, Y" uniqKey="Mimura Y">Y. Mimura</name></author><author><name sortKey="Kunert, R" uniqKey="Kunert R">R. Kunert</name></author><author><name sortKey="Zhu, P" uniqKey="Zhu P">P. Zhu</name></author><author><name sortKey="Wormald, M R" uniqKey="Wormald M">M.R. Wormald</name></author><author><name sortKey="Stanfield, R L" uniqKey="Stanfield R">R.L. Stanfield</name></author><author><name sortKey="Roux, K H" uniqKey="Roux K">K.H. Roux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Burton, D R" uniqKey="Burton D">D.R. Burton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scanlan, C N" uniqKey="Scanlan C">C.N. Scanlan</name></author><author><name sortKey="Pantophlet, R" uniqKey="Pantophlet R">R. Pantophlet</name></author><author><name sortKey="Wormald, M R" uniqKey="Wormald M">M.R. Wormald</name></author><author><name sortKey="Ollmann Saphire, E" uniqKey="Ollmann Saphire E">E. Ollmann Saphire</name></author><author><name sortKey="Stanfield, R" uniqKey="Stanfield R">R. Stanfield</name></author><author><name sortKey="Wilson, I A" uniqKey="Wilson I">I.A. Wilson</name></author><author><name sortKey="Katinger, H" uniqKey="Katinger H">H. Katinger</name></author><author><name sortKey="Dwek, R A" uniqKey="Dwek R">R.A. Dwek</name></author><author><name sortKey="Rudd, P M" uniqKey="Rudd P">P.M. Rudd</name></author><author><name sortKey="Burton, D R" uniqKey="Burton D">D.R. Burton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanders, R W" uniqKey="Sanders R">R.W. Sanders</name></author><author><name sortKey="Venturi, M" uniqKey="Venturi M">M. Venturi</name></author><author><name sortKey="Schiffner, L" uniqKey="Schiffner L">L. Schiffner</name></author><author><name sortKey="Kalyanaraman, R" uniqKey="Kalyanaraman R">R. Kalyanaraman</name></author><author><name sortKey="Katinger, H" uniqKey="Katinger H">H. Katinger</name></author><author><name sortKey="Lloyd, K O" uniqKey="Lloyd K">K.O. Lloyd</name></author><author><name sortKey="Kwong, P D" uniqKey="Kwong P">P.D. Kwong</name></author><author><name sortKey="Moore, J P" uniqKey="Moore J">J.P. Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Simek, M D" uniqKey="Simek M">M.D. Simek</name></author><author><name sortKey="Priddy, F" uniqKey="Priddy F">F. Priddy</name></author><author><name sortKey="Gach, J S" uniqKey="Gach J">J.S. Gach</name></author><author><name sortKey="Wagner, D" uniqKey="Wagner D">D. Wagner</name></author><author><name sortKey="Zwick, M B" uniqKey="Zwick M">M.B. Zwick</name></author><author><name sortKey="Phogat, S K" uniqKey="Phogat S">S.K. Phogat</name></author><author><name sortKey="Poignard, P" uniqKey="Poignard P">P. Poignard</name></author><author><name sortKey="Burton, D R" uniqKey="Burton D">D.R. Burton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pejchal, R" uniqKey="Pejchal R">R. Pejchal</name></author><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Stanfield, R L" uniqKey="Stanfield R">R.L. Stanfield</name></author><author><name sortKey="Phogat, S K" uniqKey="Phogat S">S.K. Phogat</name></author><author><name sortKey="Koff, W C" uniqKey="Koff W">W.C. Koff</name></author><author><name sortKey="Poignard, P" uniqKey="Poignard P">P. Poignard</name></author><author><name sortKey="Burton, D R" uniqKey="Burton D">D.R. Burton</name></author><author><name sortKey="Wilson, I A" uniqKey="Wilson I">I.A. Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Falkowska, E" uniqKey="Falkowska E">E. Falkowska</name></author><author><name sortKey="Ramos, A" uniqKey="Ramos A">A. Ramos</name></author><author><name sortKey="Feng, Y" uniqKey="Feng Y">Y. Feng</name></author><author><name sortKey="Zhou, T" uniqKey="Zhou T">T. Zhou</name></author><author><name sortKey="Moquin, S" uniqKey="Moquin S">S. Moquin</name></author><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Wu, X" uniqKey="Wu X">X. Wu</name></author><author><name sortKey="Seaman, M S" uniqKey="Seaman M">M.S. Seaman</name></author><author><name sortKey="Wrin, T" uniqKey="Wrin T">T. Wrin</name></author><author><name sortKey="Kwong, P D" uniqKey="Kwong P">P.D. Kwong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Sok, D" uniqKey="Sok D">D. Sok</name></author><author><name sortKey="Nishimura, Y" uniqKey="Nishimura Y">Y. Nishimura</name></author><author><name sortKey="Donau, O" uniqKey="Donau O">O. Donau</name></author><author><name sortKey="Sadjadpour, R" uniqKey="Sadjadpour R">R. Sadjadpour</name></author><author><name sortKey="Gautam, R" uniqKey="Gautam R">R. Gautam</name></author><author><name sortKey="Shingai, M" uniqKey="Shingai M">M. Shingai</name></author><author><name sortKey="Pejchal, R" uniqKey="Pejchal R">R. Pejchal</name></author><author><name sortKey="Ramos, A" uniqKey="Ramos A">A. Ramos</name></author><author><name sortKey="Simek, M D" uniqKey="Simek M">M.D. Simek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pejchal, R" uniqKey="Pejchal R">R. Pejchal</name></author><author><name sortKey="Doores, K J" uniqKey="Doores K">K.J. Doores</name></author><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Khayat, R" uniqKey="Khayat R">R. Khayat</name></author><author><name sortKey="Huang, P S" uniqKey="Huang P">P.S. Huang</name></author><author><name sortKey="Wang, S K" uniqKey="Wang S">S.K. Wang</name></author><author><name sortKey="Stanfield, R L" uniqKey="Stanfield R">R.L. Stanfield</name></author><author><name sortKey="Julien, J P" uniqKey="Julien J">J.P. Julien</name></author><author><name sortKey="Ramos, A" uniqKey="Ramos A">A. Ramos</name></author><author><name sortKey="Crispin, M" uniqKey="Crispin M">M. Crispin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walker, L M" uniqKey="Walker L">L.M. Walker</name></author><author><name sortKey="Huber, M" uniqKey="Huber M">M. Huber</name></author><author><name sortKey="Doores, K J" uniqKey="Doores K">K.J. Doores</name></author><author><name sortKey="Falkowska, E" uniqKey="Falkowska E">E. Falkowska</name></author><author><name sortKey="Pejchal, R" uniqKey="Pejchal R">R. Pejchal</name></author><author><name sortKey="Julien, J P" uniqKey="Julien J">J.P. Julien</name></author><author><name sortKey="Wang, S K" uniqKey="Wang S">S.K. Wang</name></author><author><name sortKey="Ramos, A" uniqKey="Ramos A">A. Ramos</name></author><author><name sortKey="Chan Hui, P Y" uniqKey="Chan Hui P">P.Y. Chan-Hui</name></author><author><name sortKey="Moyle, M" uniqKey="Moyle M">M. Moyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L X" uniqKey="Wang L">L.X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trkola, A" uniqKey="Trkola A">A. Trkola</name></author><author><name sortKey="Purtscher, M" uniqKey="Purtscher M">M. Purtscher</name></author><author><name sortKey="Muster, T" uniqKey="Muster T">T. Muster</name></author><author><name sortKey="Ballaun, C" uniqKey="Ballaun C">C. Ballaun</name></author><author><name sortKey="Buchacher, A" uniqKey="Buchacher A">A. Buchacher</name></author><author><name sortKey="Sullivan, N" uniqKey="Sullivan N">N. Sullivan</name></author><author><name sortKey="Srinivasan, K" uniqKey="Srinivasan K">K. Srinivasan</name></author><author><name sortKey="Sodroski, J" uniqKey="Sodroski J">J. Sodroski</name></author><author><name sortKey="Moore, J P" uniqKey="Moore J">J.P. Moore</name></author><author><name sortKey="Katinger, H" uniqKey="Katinger H">H. Katinger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mclellan, J S" uniqKey="Mclellan J">J.S. McLellan</name></author><author><name sortKey="Pancera, M" uniqKey="Pancera M">M. Pancera</name></author><author><name sortKey="Carrico, C" uniqKey="Carrico C">C. Carrico</name></author><author><name sortKey="Gorman, J" uniqKey="Gorman J">J. Gorman</name></author><author><name sortKey="Julien, J P" uniqKey="Julien J">J.P. Julien</name></author><author><name sortKey="Khayat, R" uniqKey="Khayat R">R. Khayat</name></author><author><name sortKey="Louder, R" uniqKey="Louder R">R. Louder</name></author><author><name sortKey="Pejchal, R" uniqKey="Pejchal R">R. Pejchal</name></author><author><name sortKey="Sastry, M" uniqKey="Sastry M">M. Sastry</name></author><author><name sortKey="Dai, K" uniqKey="Dai K">K. Dai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Lu, J" uniqKey="Lu J">J. Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M. Eickmann</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name></author><author><name sortKey="Klenk, H D" uniqKey="Klenk H">H.D. Klenk</name></author><author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W. Doerr</name></author><author><name sortKey="Stadler, K" uniqKey="Stadler K">K. Stadler</name></author><author><name sortKey="Censini, S" uniqKey="Censini S">S. Censini</name></author><author><name sortKey="Guidotti, S" uniqKey="Guidotti S">S. Guidotti</name></author><author><name sortKey="Masignani, V" uniqKey="Masignani V">V. Masignani</name></author><author><name sortKey="Scarselli, M" uniqKey="Scarselli M">M. Scarselli</name></author><author><name sortKey="Mora, M" uniqKey="Mora M">M. Mora</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name></author><author><name sortKey="Jones, S J" uniqKey="Jones S">S.J. Jones</name></author><author><name sortKey="Astell, C R" uniqKey="Astell C">C.R. Astell</name></author><author><name sortKey="Holt, R A" uniqKey="Holt R">R.A. Holt</name></author><author><name sortKey="Brooks Wilson, A" uniqKey="Brooks Wilson A">A. Brooks-Wilson</name></author><author><name sortKey="Butterfield, Y S" uniqKey="Butterfield Y">Y.S. Butterfield</name></author><author><name sortKey="Khattra, J" uniqKey="Khattra J">J. Khattra</name></author><author><name sortKey="Asano, J K" uniqKey="Asano J">J.K. Asano</name></author><author><name sortKey="Barber, S A" uniqKey="Barber S">S.A. Barber</name></author><author><name sortKey="Chan, S Y" uniqKey="Chan S">S.Y. Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name></author><author><name sortKey="Oberste, M S" uniqKey="Oberste M">M.S. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S.S. Monroe</name></author><author><name sortKey="Nix, W A" uniqKey="Nix W">W.A. Nix</name></author><author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R. Campagnoli</name></author><author><name sortKey="Icenogle, J P" uniqKey="Icenogle J">J.P. Icenogle</name></author><author><name sortKey="Penaranda, S" uniqKey="Penaranda S">S. Penaranda</name></author><author><name sortKey="Bankamp, B" uniqKey="Bankamp B">B. Bankamp</name></author><author><name sortKey="Maher, K" uniqKey="Maher K">K. Maher</name></author><author><name sortKey="Chen, M H" uniqKey="Chen M">M.H. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiao, X" uniqKey="Xiao X">X. Xiao</name></author><author><name sortKey="Chakraborti, S" uniqKey="Chakraborti S">S. Chakraborti</name></author><author><name sortKey="Dimitrov, A S" uniqKey="Dimitrov A">A.S. Dimitrov</name></author><author><name sortKey="Gramatikoff, K" uniqKey="Gramatikoff K">K. Gramatikoff</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ritchie, G" uniqKey="Ritchie G">G. Ritchie</name></author><author><name sortKey="Harvey, D J" uniqKey="Harvey D">D.J. Harvey</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F. Feldmann</name></author><author><name sortKey="Stroeher, U" uniqKey="Stroeher U">U. Stroeher</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="Royle, L" uniqKey="Royle L">L. Royle</name></author><author><name sortKey="Dwek, R A" uniqKey="Dwek R">R.A. Dwek</name></author><author><name sortKey="Rudd, P M" uniqKey="Rudd P">P.M. Rudd</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J. Balzarini</name></author><author><name sortKey="Schols, D" uniqKey="Schols D">D. Schols</name></author><author><name sortKey="Neyts, J" uniqKey="Neyts J">J. Neyts</name></author><author><name sortKey="Van Damme, E" uniqKey="Van Damme E">E. Van Damme</name></author><author><name sortKey="Peumans, W" uniqKey="Peumans W">W. Peumans</name></author><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E. de Clercq</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J. Balzarini</name></author><author><name sortKey="Van Laethem, K" uniqKey="Van Laethem K">K. van Laethem</name></author><author><name sortKey="Hatse, S" uniqKey="Hatse S">S. Hatse</name></author><author><name sortKey="Froeyen, M" uniqKey="Froeyen M">M. Froeyen</name></author><author><name sortKey="Van Damme, E" uniqKey="Van Damme E">E. van Damme</name></author><author><name sortKey="Bolmstedt, A" uniqKey="Bolmstedt A">A. Bolmstedt</name></author><author><name sortKey="Peumans, W" uniqKey="Peumans W">W. Peumans</name></author><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E. de Clercq</name></author><author><name sortKey="Schols, D" uniqKey="Schols D">D. Schols</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J. Balzarini</name></author><author><name sortKey="Van Laethem, K" uniqKey="Van Laethem K">K. van Laethem</name></author><author><name sortKey="Hatse, S" uniqKey="Hatse S">S. Hatse</name></author><author><name sortKey="Vermeire, K" uniqKey="Vermeire K">K. Vermeire</name></author><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E. de Clercq</name></author><author><name sortKey="Peumans, W" uniqKey="Peumans W">W. Peumans</name></author><author><name sortKey="Van Damme, E" uniqKey="Van Damme E">E. van Damme</name></author><author><name sortKey="Vandamme, A M" uniqKey="Vandamme A">A.M. Vandamme</name></author><author><name sortKey="Bolmstedt, A" uniqKey="Bolmstedt A">A. Bolmstedt</name></author><author><name sortKey="Schols, D" uniqKey="Schols D">D. Schols</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J. Balzarini</name></author><author><name sortKey="Hatse, S" uniqKey="Hatse S">S. Hatse</name></author><author><name sortKey="Vermeire, K" uniqKey="Vermeire K">K. Vermeire</name></author><author><name sortKey="Princen, K" uniqKey="Princen K">K. Princen</name></author><author><name sortKey="Aquaro, S" uniqKey="Aquaro S">S. Aquaro</name></author><author><name sortKey="Perno, C F" uniqKey="Perno C">C.F. Perno</name></author><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E. de Clercq</name></author><author><name sortKey="Egberink, H" uniqKey="Egberink H">H. Egberink</name></author><author><name sortKey="Vanden Mooter, G" uniqKey="Vanden Mooter G">G. Vanden Mooter</name></author><author><name sortKey="Peumans, W" uniqKey="Peumans W">W. Peumans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E. Keyaerts</name></author><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L. Vijgen</name></author><author><name sortKey="Pannecouque, C" uniqKey="Pannecouque C">C. Pannecouque</name></author><author><name sortKey="Van Damme, E" uniqKey="Van Damme E">E. van Damme</name></author><author><name sortKey="Peumans, W" uniqKey="Peumans W">W. Peumans</name></author><author><name sortKey="Egberink, H" uniqKey="Egberink H">H. Egberink</name></author><author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J. Balzarini</name></author><author><name sortKey="Van Ranst, M" uniqKey="Van Ranst M">M. van Ranst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Q" uniqKey="Yang Q">Q. Yang</name></author><author><name sortKey="Li, C" uniqKey="Li C">C. Li</name></author><author><name sortKey="Wei, Y" uniqKey="Wei Y">Y. Wei</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W. Huang</name></author><author><name sortKey="Wang, L X" uniqKey="Wang L">L.X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scanlan, C N" uniqKey="Scanlan C">C.N. Scanlan</name></author><author><name sortKey="Ritchie, G E" uniqKey="Ritchie G">G.E. Ritchie</name></author><author><name sortKey="Baruah, K" uniqKey="Baruah K">K. Baruah</name></author><author><name sortKey="Crispin, M" uniqKey="Crispin M">M. Crispin</name></author><author><name sortKey="Harvey, D J" uniqKey="Harvey D">D.J. Harvey</name></author><author><name sortKey="Singer, B B" uniqKey="Singer B">B.B. Singer</name></author><author><name sortKey="Lucka, L" uniqKey="Lucka L">L. Lucka</name></author><author><name sortKey="Wormald, M R" uniqKey="Wormald M">M.R. Wormald</name></author><author><name sortKey="Wentworth, P" uniqKey="Wentworth P">P. Wentworth</name></author><author><name sortKey="Zitzmann, N" uniqKey="Zitzmann N">N. Zitzmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doores, K J" uniqKey="Doores K">K.J. Doores</name></author><author><name sortKey="Bonomelli, C" uniqKey="Bonomelli C">C. Bonomelli</name></author><author><name sortKey="Harvey, D J" uniqKey="Harvey D">D.J. Harvey</name></author><author><name sortKey="Vasiljevic, S" uniqKey="Vasiljevic S">S. Vasiljevic</name></author><author><name sortKey="Dwek, R A" uniqKey="Dwek R">R.A. Dwek</name></author><author><name sortKey="Burton, D R" uniqKey="Burton D">D.R. Burton</name></author><author><name sortKey="Crispin, M" uniqKey="Crispin M">M. Crispin</name></author><author><name sortKey="Scanlan, C N" uniqKey="Scanlan C">C.N. Scanlan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rambaruth, N D" uniqKey="Rambaruth N">N.D. Rambaruth</name></author><author><name sortKey="Greenwell, P" uniqKey="Greenwell P">P. Greenwell</name></author><author><name sortKey="Dwek, M V" uniqKey="Dwek M">M.V. Dwek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tempel, W" uniqKey="Tempel W">W. Tempel</name></author><author><name sortKey="Tschampel, S" uniqKey="Tschampel S">S. Tschampel</name></author><author><name sortKey="Woods, R J" uniqKey="Woods R">R.J. Woods</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Galili, U" uniqKey="Galili U">U. Galili</name></author><author><name sortKey="Clark, M R" uniqKey="Clark M">M.R. Clark</name></author><author><name sortKey="Shohet, S B" uniqKey="Shohet S">S.B. Shohet</name></author><author><name sortKey="Buehler, J" uniqKey="Buehler J">J. Buehler</name></author><author><name sortKey="Macher, B A" uniqKey="Macher B">B.A. Macher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Bhat, R" uniqKey="Bhat R">R. Bhat</name></author><author><name sortKey="Sobel, R A" uniqKey="Sobel R">R.A. Sobel</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W. Huang</name></author><author><name sortKey="Wang, L X" uniqKey="Wang L">L.X. Wang</name></author><author><name sortKey="Olsson, T" uniqKey="Olsson T">T. Olsson</name></author><author><name sortKey="Steinman, L" uniqKey="Steinman L">L. Steinman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benko, D M" uniqKey="Benko D">D.M. Benko</name></author><author><name sortKey="Gibson, W" uniqKey="Gibson W">W. Gibson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weekes, M P" uniqKey="Weekes M">M.P. Weekes</name></author><author><name sortKey="Tomasec, P" uniqKey="Tomasec P">P. Tomasec</name></author><author><name sortKey="Huttlin, E L" uniqKey="Huttlin E">E.L. Huttlin</name></author><author><name sortKey="Fielding, C A" uniqKey="Fielding C">C.A. Fielding</name></author><author><name sortKey="Nusinow, D" uniqKey="Nusinow D">D. Nusinow</name></author><author><name sortKey="Stanton, R J" uniqKey="Stanton R">R.J. Stanton</name></author><author><name sortKey="Wang, E C" uniqKey="Wang E">E.C. Wang</name></author><author><name sortKey="Aicheler, R" uniqKey="Aicheler R">R. Aicheler</name></author><author><name sortKey="Murrell, I" uniqKey="Murrell I">I. Murrell</name></author><author><name sortKey="Wilkinson, G W" uniqKey="Wilkinson G">G.W. Wilkinson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Dafik, L" uniqKey="Dafik L">L. Dafik</name></author><author><name sortKey="Nolley, R" uniqKey="Nolley R">R. Nolley</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W. Huang</name></author><author><name sortKey="Wolfinger, R D" uniqKey="Wolfinger R">R.D. Wolfinger</name></author><author><name sortKey="Wang, L X" uniqKey="Wang L">L.X. Wang</name></author><author><name sortKey="Peehl, D M" uniqKey="Peehl D">D.M. Peehl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author><author><name sortKey="Trummer, B J" uniqKey="Trummer B">B.J. Trummer</name></author><author><name sortKey="Deng, C" uniqKey="Deng C">C. Deng</name></author><author><name sortKey="Wang, A" uniqKey="Wang A">A. Wang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Molecules</journal-id><journal-id journal-id-type="iso-abbrev">Molecules</journal-id><journal-id journal-id-type="publisher-id">molecules</journal-id><journal-title-group><journal-title>Molecules</journal-title></journal-title-group><issn pub-type="epub">1420-3049</issn><publisher><publisher-name>MDPI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25774492</article-id><article-id pub-id-type="pmc">4633014</article-id><article-id pub-id-type="doi">10.3390/molecules20034610</article-id><article-id pub-id-type="publisher-id">molecules-20-04610</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Targeting N-Glycan Cryptic Sugar Moieties for Broad-Spectrum Virus Neutralization: Progress in Identifying Conserved Molecular Targets in Viruses of Distinct Phylogenetic Origins</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Denong</given-names></name><xref ref-type="aff" rid="af1-molecules-20-04610">1</xref><xref rid="c1-molecules-20-04610" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Jin</given-names></name><xref ref-type="aff" rid="af1-molecules-20-04610">1</xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Jiulai</given-names></name><xref ref-type="aff" rid="af2-molecules-20-04610">2</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Lai-Xi</given-names></name><xref ref-type="aff" rid="af3-molecules-20-04610">3</xref><xref ref-type="aff" rid="af4-molecules-20-04610">4</xref></contrib></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Ferro</surname><given-names>Vito</given-names></name><role>Academic Editor</role></contrib><contrib contrib-type="editor"><name><surname>Velasco-Torrijos</surname><given-names>Trinidad</given-names></name><role>Academic Editor</role></contrib></contrib-group><aff id="af1-molecules-20-04610"><label>1</label>Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail:<email>jiafengjushi@live.com</email></aff><aff id="af2-molecules-20-04610"><label>2</label>Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China; E-Mail:<email>tangjiulai8888@21cn.com</email></aff><aff id="af3-molecules-20-04610"><label>3</label>Institute of Human Virology, Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; E-Mail:<email>LWang@ihv.umaryland.edu</email></aff><aff id="af4-molecules-20-04610"><label>4</label>Department of Chemistry and Biochemistry, University of Maryland at College Park, College Park, MD 20742, USA</aff><author-notes><corresp id="c1-molecules-20-04610"><label>*</label>Author to whom correspondence should be addressed; E-Mail: <email>denong.wang@sri.com</email>; Tel.: +1-650-859-2789; Fax: +1-650-859-3153.</corresp></author-notes><pub-date pub-type="epub"><day>12</day><month>3</month><year>2015</year></pub-date><pub-date pub-type="collection"><month>3</month><year>2015</year></pub-date><volume>20</volume><issue>3</issue><fpage>4610</fpage><lpage>4622</lpage><history><date date-type="received"><day>20</day><month>1</month><year>2015</year></date><date date-type="accepted"><day>09</day><month>3</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 by the authors.</copyright-statement><copyright-year>2015</copyright-year><license license-type="open-access"><license-p>Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>).</license-p></license></permissions><abstract><p>Identifying molecular targets for eliciting broadly virus-neutralizing antibodies is one of the key steps toward development of vaccines against emerging viral pathogens. Owing to genomic and somatic diversities among viral species, identifying protein targets for broad-spectrum virus neutralization is highly challenging even for the same virus, such as HIV-1. However, viruses rely on host glycosylation machineries to synthesize and express glycans and, thereby, may display common carbohydrate moieties. Thus, exploring glycan-binding profiles of broad-spectrum virus-neutralizing agents may provide key information to uncover the carbohydrate-based virus-neutralizing epitopes. In this study, we characterized two broadly HIV-neutralizing agents, human monoclonal antibody 2G12 and Galanthus nivalis lectin (GNA), for their viral targeting activities. Although these agents were known to be specific for oligomannosyl antigens, they differ strikingly in virus-binding activities. The former is HIV-1 specific; the latter is broadly reactive and is able to neutralize viruses of distinct phylogenetic origins, such as HIV-1, severe acute respiratory syndrome coronavirus (SARS-CoV), and human cytomegalovirus (HCMV). In carbohydrate microarray analyses, we explored the molecular basis underlying the striking differences in the spectrum of anti-virus activities of the two probes. Unlike 2G12, which is strictly specific for the high-density Man<sub>9</sub>GlcNAc<sub>2</sub>Asn (Man9)-clusters, GNA recognizes a number of N-glycan cryptic sugar moieties. These include not only the known oligomannosyl antigens but also previously unrecognized tri-antennary or multi-valent GlcNAc-terminating N-glycan epitopes (Tri/m-Gn). These findings highlight the potential of N-glycan cryptic sugar moieties as conserved targets for broad-spectrum virus neutralization and suggest the GNA-model of glycan-binding warrants focused investigation.</p></abstract><kwd-group><kwd>carbohydrate microarrays</kwd><kwd>oligomannoses</kwd><kwd>AGOR</kwd><kwd>ASOR</kwd><kwd>GNA</kwd><kwd>2G12</kwd><kwd>HIV-1</kwd><kwd>HCMV</kwd><kwd>SARS-CoV</kwd></kwd-group></article-meta></front><body><sec><title>1. Introduction</title><p>Developing broad-range virus-neutralizing antibodies (bnAbs) requires identifying the immunological targets that are conserved among the targeted viruses and are suitable for antibody targeting. Discovery of oligomannosyl moieties as targets of bnAbs against HIV-1 has stimulated substantial interest in carbohydrate moieties as vaccine candidates [<xref rid="B1-molecules-20-04610" ref-type="bibr">1</xref>,<xref rid="B2-molecules-20-04610" ref-type="bibr">2</xref>,<xref rid="B3-molecules-20-04610" ref-type="bibr">3</xref>,<xref rid="B4-molecules-20-04610" ref-type="bibr">4</xref>,<xref rid="B5-molecules-20-04610" ref-type="bibr">5</xref>,<xref rid="B6-molecules-20-04610" ref-type="bibr">6</xref>,<xref rid="B7-molecules-20-04610" ref-type="bibr">7</xref>,<xref rid="B8-molecules-20-04610" ref-type="bibr">8</xref>,<xref rid="B9-molecules-20-04610" ref-type="bibr">9</xref>,<xref rid="B10-molecules-20-04610" ref-type="bibr">10</xref>,<xref rid="B11-molecules-20-04610" ref-type="bibr">11</xref>]. The heavy glycosylation of the HIV envelope with oligomannoses appears to be a defense mechanism for the virus to evade host immune recognition of protein-based neutralizing epitopes. However, isolation of a number of glycan-dependent HIV bnAbs from HIV-infected individuals, such as 2G12, PG9/PG16, and other potent bnAbs [<xref rid="B1-molecules-20-04610" ref-type="bibr">1</xref>,<xref rid="B5-molecules-20-04610" ref-type="bibr">5</xref>,<xref rid="B6-molecules-20-04610" ref-type="bibr">6</xref>,<xref rid="B10-molecules-20-04610" ref-type="bibr">10</xref>,<xref rid="B12-molecules-20-04610" ref-type="bibr">12</xref>,<xref rid="B13-molecules-20-04610" ref-type="bibr">13</xref>], strongly suggests that the “glycan shield” of HIV virions may also display important targets for immunological intervention against viral infections.</p><p>Like HIV-1, virtually all human viruses decorate their virions with carbohydrate moieties. Thus, the potential of viral carbohydrates as immunological targets for other viral pathogens warrants exploration. One intriguing question is whether human viruses of distinct phylogenetic origins, such as HIV-1 and SARS-CoV, may display conserved glycan targets that are suitable for broad virus neutralization. Availability of bnAbs targeting such conserved glycans is clearly important for improving a biodefensive public health response to unexpected viral epidemics.</p><p>In response to the 2003 SARS epidemic, our team introduced carbohydrate microarrays to study SARS-CoV-elicited anti-glycan antibodies [<xref rid="B14-molecules-20-04610" ref-type="bibr">14</xref>]. In essence, we characterized the carbohydrate-binding activity of the SARS-CoV-neutralizing antibodies elicited by immunizing horses with an inactivated SARS-CoV vaccine. In these horse antibodies, we detected unexpected auto-antibody reactivity specific for the carbohydrate moieties of an abundant human serum glycoprotein, asialo-orosomucoid (ASOR). The targeted carbohydrates are tri-antennary type II (Galβ1→4GlcNAc) or multi-valent type II (Tri/m-II) sugar moieties.</p><p>The N-glycosylation pathway has potential to generate numerous cryptic N-glycans of distinct structural characteristics. The spike glycoprotein of SARS-CoV has 23 potential N-linked glycosylation sites [<xref rid="B15-molecules-20-04610" ref-type="bibr">15</xref>,<xref rid="B16-molecules-20-04610" ref-type="bibr">16</xref>,<xref rid="B17-molecules-20-04610" ref-type="bibr">17</xref>,<xref rid="B18-molecules-20-04610" ref-type="bibr">18</xref>]. Ritchie <italic>et al.</italic> determined the glycan profiles of the spike protein produced by monkey Vero-E6 cells [<xref rid="B19-molecules-20-04610" ref-type="bibr">19</xref>]. Its major glycans include high-mannose (Man<sub>5-9</sub>GlcNAc<sub>2</sub>), hybrid, and bi-, tri- and tetra-antennary complex carbohydrates with and without bisecting GlcNAc and core fucose. Interestingly, sialylation was negligible in the spike proteins produced by monkey Vero-E6 cells, which led to exposure of terminal galactoses in Tri/m-II glyco-determinants. Thus, induction of anti-ASOR auto-antibodies by inactivated SARS-CoV can be attributed to the fact that ASOR and the SARS-CoV spike glycoprotein commonly express the Tri/m-II cryptic glyco-determinants. Of note, this structural glycomics study revealed that SARS-CoV also expresses the high-mannose series of carbohydrate structures as its major glycan moieties. Thus, despite the fact that the two viruses differ in their general glycan profiles, they commonly overexpress oligomannosyl cores of N-glycans.</p><p>A significant number of virus-neutralizing agents target oligomannosyl moieties. Notably, these include mAb 2G12 and lectin GNA. However, the two probes represent distinct classes of virus-neutralizing agents. The former is “mono-specific” for HIV-1; the latter is “pauci reactive”, being a potent neutralizer for several viruses, including at least HIV-1, HCMV, and SARS-CoV [<xref rid="B20-molecules-20-04610" ref-type="bibr">20</xref>,<xref rid="B21-molecules-20-04610" ref-type="bibr">21</xref>,<xref rid="B22-molecules-20-04610" ref-type="bibr">22</xref>,<xref rid="B23-molecules-20-04610" ref-type="bibr">23</xref>,<xref rid="B24-molecules-20-04610" ref-type="bibr">24</xref>]. Given the spectrum of anti-virus activities, GNA may serve as a model for exploration of broad-spectrum virus-neutralizing epitopes. An essential step toward this goal is to identify the natural ligands of GNA that are preserved among GNA-targeted viral pathogens, which is the focus of this study.</p></sec><sec><title>2. Results and Discussion</title><p>We reasoned that exploring glycan-binding profiles of broadly virus-neutralizing agents may provide key information to uncover the carbohydrate-based viral neutralization epitopes. In the first set of the experiments, we verified that the preparations of GNA or 2G12 we utilized recognize corresponding epitopes presented by the native viral antigens. Subsequently, we performed a comparative carbohydrate microarray analysis to characterize the glycan-binding profiles of 2G12 and GNA and to pinpoint specific glyco-epitopes they recognize.</p><sec><title>2.1. Detection of GNA- or 2G12-Epitopes in the Native Viral Antigen Preparations</title><p>In <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>A,B, we examined detection of GNA- and 2G12-epitopes in HIV-1 gp120 glycoproteins. To preserve the native glyco-epitopes, we produced two gp120 preparations in HEK293 cells, <italic>i.e.</italic>, Bal-gp120-Man9 and Bal-gp120. Bal-gp120-Man9 was expressed in HEK293 cells in the presence of kifunensine (2 µg/mL), a potent α-mannosidase I inhibitor, following the previously described procedures [<xref rid="B25-molecules-20-04610" ref-type="bibr">25</xref>,<xref rid="B26-molecules-20-04610" ref-type="bibr">26</xref>]. The use of the α-mannosidase I inhibitor allows the enrichment of high-mannose type (Man9) glycoform through blocking further glycosylation processing to complex or hybrid carbohydrates. Thus, Bal-gp120-Man9 was produced to resemble the native envelope spikes of HIV, which are almost entirely coated with oligomannose antigens [<xref rid="B27-molecules-20-04610" ref-type="bibr">27</xref>]. These proteins were applied on ELISA plates at 1.0 µg/mL, and the glycan staining was performed in comparison with lectins Phaseolus vulgaris-L lectin (PHA-L) and Sambucus nigra I agglutinin (SNA-I). With GNA and 2G12 targeting oligomannosyl epitopes, PHA-L for Tri/m-II cryptic epitopes, and SNA-I recognizing α2-6-linked Neu5Ac residues, this panel of probes monitors expression of layers of cryptic glyco-epitopes as schematically shown in <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>A.</p><p>This assay demonstrated that both Bal-gp120-Man9 (<xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>A) and Bal-gp120 (<xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>B) were strongly positive with GNA and 2G12. PHA-L was selectively reactive with Bal-gp120 but not with Bal-gp120-Man9. This PHA-L-differential staining pattern reflects the effective blockage of biosynthesis of Tri/m-II complex moieties during Bal-gp120-Man9 production as we initially planned. SNA-1 was negative to both glycoproteins under the same staining condition.</p><fig id="molecules-20-04610-f001" position="float"><label>Figure 1</label><caption><p>ELISA assays validated GNA-epitope expression by native viral antigens derived from HIV-1, SARS-CoV, or HCMV. (<bold>A</bold>,<bold>B</bold>) HIV-1-specific ELISA with Bal-gp120-Man9 (A) or Bal-gp120 (B) coated at 1.0 µg/mL; (<bold>C</bold>) SARS-CoV-specific ELISA, which was optimized for preserving native viral antigens; (<bold>D</bold>) HCMV-specific ELISA with purified virus at 1.0 µg/mL; and (<bold>E</bold>) Man9-KLH conjugate was applied at 10.0 µg/mL for coating. ELISA results were presented without background subtraction in <bold>A</bold>–<bold>C</bold> or with background subtraction in D and E. Lectins were applied at the concentrations (µg/mL) as specified. The ELISA data shown here are representative results of multiple assays.</p></caption><graphic xlink:href="molecules-20-04610-g001"></graphic></fig><p>We further examined whether SARS-CoV expresses the native GNA-epitopes and PHA-L-epitopes. In <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>C, the ELISA plates were coated with the native SARS-CoV antigens that were expressed by Vero E6 cells. These plates were treated with a disinfecting fixing agent and gamma-irradiated to inactivate infectious virus particles while preserving antigenic structures of SARS-CoV. Lectins were applied at the concentrations specified in the figures.</p><p>This assay demonstrates that the PHA-L-epitopes were readily detected in the SARS-CoV-coated ELISA plates as we previously observed [<xref rid="B14-molecules-20-04610" ref-type="bibr">14</xref>]. Moreover, it shows that GNA is strongly positive with SARS-CoV. By contrast, other Gal/GalNAc-reactive lectins, including <italic>Helix pomatia</italic> (HPA) and <italic>Griffonia (Bandeiraea) simplicifolia</italic>-I (GS-I), were only marginally reactive with SARS-CoV antigens. HPA is specific for GalNAc- and O-GlcNAc-moieties [<xref rid="B28-molecules-20-04610" ref-type="bibr">28</xref>]; GS-I recognizes the α-Gal-epitope [<xref rid="B29-molecules-20-04610" ref-type="bibr">29</xref>,<xref rid="B30-molecules-20-04610" ref-type="bibr">30</xref>]. The latter is conserved in many microorganisms but is negligible in SARS-CoV as expected. </p><p>In <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>D, we examined whether HCMV expresses oligomannosyl epitopes using GNA and concanavalin A (Con A) in comparison with other lectins of different specificities. In this experiment, ELISA was coated with purified virus (AD169, Creative Diagnostics, New York, NY, USA) at protein concentration approximately 1.0 µg/mL for lectin-screening. The two anti-mannose agents differ in epitope-binding specificities. GNA is specific for the Manα1,3Man and Manα1,6Man moieties of oligomannoses; Con A recognizes terminal Manα1<bold>→</bold>moieties that are more broadly expressed by mannose-containing antigens. <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>E illustrates lectin-binding of a synthetic glycoconjugate, Man9-KLH, which bears both GNA- and Con-A-glyco-epitopes [<xref rid="B31-molecules-20-04610" ref-type="bibr">31</xref>]. Inspection of <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>D readily reveals that the relative binding activity of GNA is markedly higher than the Con-A activity in the HCMV-ELISA assay although the two mannose-specific lectins are similarly reactive with the Man9-KLH standard in the assay. Other lectins of different glycan-binding specificities show certain reactivities with HCMV lysates, which may reflect the glycome complexity of this most intricate human virus [<xref rid="B32-molecules-20-04610" ref-type="bibr">32</xref>,<xref rid="B33-molecules-20-04610" ref-type="bibr">33</xref>], which requires further study.</p><fig id="molecules-20-04610-f002" position="float"><label>Figure 2</label><caption><p>A comparative microarray analysis of the glyco-epitopes that are recognized by PHA-L, GNA, and 2G12. (<bold>A</bold>) Schematic of N-glycan “cryptic” glyco-epitopes, Tri/m-II and Tri/m-Gn, that are displayed by autoantigen, ASOR and AGOR, respectively; (<bold>B</bold>) Microarray images of PHA-L, GNA, or 2G12 staining against autoantigens, OR, ASOR, AGOR, Man9, (M9)<sub>4</sub>, and Ribonuclease B, which predominately express Man5 and Man6 (M5-6), as well as control probes, KLH, and <italic>E. coli</italic> K1. <italic>Importantly, GNA binds to AGOR but not to ASOR or OR</italic>; and (<bold>C</bold>) Microarray datasets for PHA-L (<bold>Upper</bold>), GNA (<bold>Middle</bold>), and 2G12 (<bold>Bottom</bold>), respectively. Each antigen was spotted in triplicate at given concentrations as specified. Results were compared using overlay plots of the MFIs of staining signal (<italic>blue bars</italic>) versus those of local backgrounds surrounding the antigen microarrays (<italic>red bars</italic>).</p></caption><graphic xlink:href="molecules-20-04610-g002"></graphic></fig><p>In summary, we verified expression of the native GNA-epitopes by three phylogenetically distinct viruses, HIV-1, SARS-CoV, and HCMV. In the context of a panel of lectins of different specificities, GNA was identified to have the highest relative binding activities with SARS-CoV and HCMV.</p></sec><sec><title>2.2. Carbohydrate Microarrays to Explore the Potential Glyco-Epitopes of GNA</title><p>The ability of GNA to bind and neutralize viruses of distinct phylogenetic origins unavoidably raises questions about its glycan-binding specificity and potential cross-reactivity. One plausible explanation is that these targeted viruses commonly express the defined GNA-epitopes of Manα1,3Man and Manα1,6Man moieties. However, the selective GNA-positive staining of HCMV without parallel Con A reactivity (<xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>D) suggests an alternative possibility, <italic>i.e.</italic>, presence of other carbohydrate moieties that are negative or weakly reactive with Con A but strongly reactive with GNA. A carbohydrate antigen with such differential reactivity between GNA and Con A is a yeast-derived phosphomannan polysaccharide (P-Man), which is nevertheless not present in the viral glycome [<xref rid="B31-molecules-20-04610" ref-type="bibr">31</xref>,<xref rid="B34-molecules-20-04610" ref-type="bibr">34</xref>].</p><p>To support exploration of the potential GNA glyco-epitopes in this study, we produced a set of comprehensive antigen microarrays, which include a large-panel of carbohydrates, lipids/liposomes, and protein antigens (<xref ref-type="app" rid="app1-molecules-20-04610">Supplementary Table S1</xref>). Key compounds supporting glyco-epitope analysis include the following:
<list list-type="simple"><list-item><label>(1)</label><p>Orosomucoid (OR) (Neu5Ac), ASOR (Tri/m-II), and Agalacto-OR (AGOR) (Tri/m-Gn) (<xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>A). These autoantigens display distinct glyco-epitopes with identical protein carriers. ASOR is an asialo-derivative of OR, and AGOR is an agalacto-derivative of ASOR. They are crucial for defining binding-specificities for N-glycan cryptic epitopes, Tri/m-II, and Tri/m-Gn.</p></list-item><list-item><label>(2)</label><p>Thiolated keyhole limpet hemocyanin (KLH-SH), (Man9GlcNAc2Asn)n-KLH (Man9), and [(Man9GlcNAc2Asn)4]n-KLH (M9_2G12). The two Man9-KLH conjugates display Man9 moieties in two defined cluster configurations. M9_2G12 is highly specific for 2G12.</p></list-item><list-item><label>(3)</label><p>Ribonuclease B (RB) with Man5-6GlcNAc2Asn (M5-6) as the main glycans. </p></list-item><list-item><label>(4)</label><p>Phosphomannan (P-Man), a yeast polysaccharide. Both M5-6-RB and P-Man are known to be positive with GNA; the former but not the latter also binds to Con A. Using these microarrays, we characterized GNA, PHA-L, and 2G12 for their epitope-binding profiles. Results are summarized in <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref> and <xref ref-type="fig" rid="molecules-20-04610-f003">Figure 3</xref>.</p></list-item></list></p><p><xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>A is a schematic view of structural relationship among autoantigens, OR, ASOR, and AGOR and the glyco-epitopes they display. <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>B shows microarray images of PHA-L, GNA, or 2G12 staining against a panel of autoantigens, including OR, ASOR, AGOR, Man9, (M9)<sub>4</sub>, which stands for [(Man9GlcNAc2Asn)4]n-KLH (M9_2G12) conjugates, and M5-6-RB. The two control probes spotted are KLH and <italic>E. coli</italic> K1 polysaccharide. Microarray datasets corresponding to <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>B are shown in <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>C. As illustrated, each antigen was spotted in triplicate at given concentrations as specified. Microarray detections are shown as the mean fluorescent intensities (MFIs) of triplicate microspots for the arrays stained with PHA-L, GNA, and 2G12, respectively. Results were compared using overlay plots of the MFIs of staining signal (<italic>blue bars</italic>) <italic>versus</italic> those of local backgrounds surrounding the antigen microarrays (<italic>red bars</italic>).</p><p><xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>B,E show that PHA-L and 2G12 are specific for ASOR (Tri/m-II) and (M9)<sub>4</sub>, respectively, which is expected. However, GNA highly and selectively binds to a number of N-glycan cryptic sugar moieties, including Man9, (M9)<sub>4</sub>, M5-6, and AGOR. GNA-binding of AGOR is a novel observation. Given that GNA had no binding to ASOR (Tri/m-II), which only differs from AGOR (Tri/m-Gn) by having the terminal galactoses. This result demonstrates, therefore, that GNA is most likely specific for the Tri/m-Gn-glyco-epitopes of AGOR with exposed terminal GlcNAc moieties.</p><p>In <xref ref-type="fig" rid="molecules-20-04610-f003">Figure 3</xref> below, we illustrate the global antigen-binding profiles of GNA and 2G12 against the full panel of antigen preparations. Microarray results were plotted as the ratio of antigen-specific signals over the corresponding spots’ background readings, <italic>i.e.</italic>, GNA Ag/Bg, or 2G12 Ag/Bg. To assist a comparative analysis of binding profiles between GNA and 2G12, datasets were plotted in the same scale in the y-axis. The positive detections labeled with numbers in the graphs are AGOR (5), Man9 (9), (M9)<sub>4</sub> (11), M5-6-RB (15), and P-Man in two dilutions (17 and 18). The whole datasets for these graphs are listed in <xref ref-type="app" rid="app1-molecules-20-04610">Supplementary Table S2</xref>.</p><fig id="molecules-20-04610-f003" position="float"><label>Figure 3</label><caption><p>Carbohydrate microarrays reveal distinct models of glycan recognition by GNA and 2G12, respectively. Antigens spotted include carbohydrates (1–39), lipids/liposomes (40–91), and proteins (92–104). (<bold>Upper panel</bold>) GNA stain (1.0 µg/mL); (<bold>Bottom panel</bold>) 2G12 stain (5.0 µg/mL). Results were plotted as antigen-specific reading over background. Corresponding microarray datasets are shown in <xref ref-type="app" rid="app1-molecules-20-04610">Supplementary Table S2</xref>.</p></caption><graphic xlink:href="molecules-20-04610-g003"></graphic></fig><p>In this microarray analysis, GNA and 2G12 show minimal or no cross-relativities with irrelevant antigens but illustrate strikingly different glycan-binding profiles. 2G12 is “mono-specific” for Man9-clusters (9 and 11) as expected. By contrast, GNA is “pauci reactive” with a number of glycan targets. Its binding to the spotted oligomannose antigens (5, 9, 11, and 15) and P-Man (17 and 18) can be attributed to the known GNA-specificity for recognition of the shared Manα1,3Man and/or Manα1,6Man moieties. However, GNA-binding to AGOR, but not to ASOR or OR, indicates that GNA also specifically recognize the Tri/m-Gn-glyco-determinants displayed by AGOR. Supporting to this prediction is the fact that AGOR, ASOR, and OR are negative to other anti-mannose agents, including lectin Con A, mAbs 2G12, TM10, and polyclonal antisera that recognize oligomannosyl moieties of varies cluster configurations [<xref rid="B31-molecules-20-04610" ref-type="bibr">31</xref>,<xref rid="B35-molecules-20-04610" ref-type="bibr">35</xref>]. Molecular mechanisms underlying GNA-recognition of the Tri/m-Gn-determinants of AGOR are yet to be explored.</p></sec></sec><sec><title>3. Experimental Section</title><sec><title>3.1. Printing Protein, Carbohydrate and Lipid/Liposome Microarrays</title><p>A high-precision microarray robot (PIXSYS 5500C, Cartesian Technologies, Irvine, CA, USA) was used to spot antigen preparations onto glass slides pre-coated with nitrocellulose polymer (FAST Slides; Schleicher & Schuell, Keene, NH, USA) as described [<xref rid="B36-molecules-20-04610" ref-type="bibr">36</xref>]. The antigen preparations applied include carbohydrates, proteins/peptides, and liposomes of various compositions as listed in <xref ref-type="app" rid="app1-molecules-20-04610">Supplementary Table S1</xref>. Proteins and carbohydrates were dissolved in phosphate-buffered saline (PBS; pH 7.4) and saline (0.9% NaCl), respectively. Liposome preparations were suspended in saline (0.9% NaCl) at the concentrations specified and were printed in triplicate with spot sizes of ~150 µm and at 375-µm intervals, center to center. The printed microarrays were air-dried and stored at room temperature without desiccant before application. </p></sec><sec><title>3.2. Staining and Scanning of Microarrays</title><p>Immediately before use, the printed microarrays were rinsed with PBS, pH 7.4, with 0.05% (v/v) Tween 20 and then blocked by incubating the slides in 1% (w/v) bovine serum albumin (BSA) in PBS containing 0.05% (w/v) NaN<sub>3</sub> at room temperature (RT) for 30 min. They were then incubated at RT with 2G12 (NIH AIDS Reagent Program, Germantown, MD, USA), biotinylated PHA-L (PHA-L<sup>BI</sup>), or GNA<sup>BI</sup> (EY Laboratories, Inc., San Mateo, CA, USA) at an indicated titration in 1% (w/v) BSA in PBS containing 0.05% (w/v) NaN<sub>3</sub> and 0.05% (v/v) Tween 20. The secondary antibodies or streptavidin conjugates applied for microarray staining are specified in the figure legends. The stained slides were rinsed five times with PBS with 0.05% (v/v) Tween 20, air-dried at room temperature, and then scanned for fluorescent signals using a ScanArray5000A Microarray Scanner (PerkinElmer Life Science, Boston, MA, USA) following the manufacturer’s manual.</p></sec><sec><title>3.3. Microarray Data-Processing and Statistical Analysis</title><p>Fluorescence intensity values for each array spot and its background were calculated using ScanArray Express software (PerkinElmer Life Science, Boston, MA, USA). SAS Institute’s JMP-Genomics software package (Cary, NC, USA) was used for further microarray data processing and statistical analysis. In <xref ref-type="fig" rid="molecules-20-04610-f002">Figure 2</xref>, microarray detections are shown as the mean fluorescent intensities (MFIs) of triplicate detections captured by ScanArray 5000A for the arrays stained with PHA-L, GNA, or 2G12. Analysis of such microarray data in association with visual inspection of the microarray image provided an evaluation of reproducibility and variation of this antigen microarray technology. In <xref ref-type="fig" rid="molecules-20-04610-f003">Figure 3</xref>, microarray results were plotted as the ratio of antigen-specific signal over corresponding spots’ background reading, <italic>i.e.</italic>, GNA Ag/Bg, or 2G12 Ag/Bg. The whole microarray datasets for PHA-L, GNA, and 2G12 were presented in <xref ref-type="app" rid="app1-molecules-20-04610">Supplementary Table S2</xref>.</p></sec><sec><title>3.4. Viral Antigen Preparations and Antigen-Specific ELISA</title><p>Two HIV-1 gp120 preparations, Bal-gp120-Man9 and Bal-gp120, were produced in HEK293 cells as previously described [<xref rid="B25-molecules-20-04610" ref-type="bibr">25</xref>,<xref rid="B26-molecules-20-04610" ref-type="bibr">26</xref>]. Bal-gp120-Man9 was expressed in the presence of the α-mannosidase I inhibitor, kifunensine (2 µg/mL), to enrich high-mannose type glycoforms. A preparation of sucrose density-gradient-purified HCMV (AD169) was obtained from Creative Diagnostics (NY, NY, USA). An ELISA protocol described previously [<xref rid="B37-molecules-20-04610" ref-type="bibr">37</xref>] for detection of anti-carbohydrate antibodies was followed with minor modifications. In <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>A–E, antigen preparations were diluted in 0.1 M sodium bicarbonate buffer solution, pH 9.6, for coating on ELISA microplates (NUNC, MaxiSorp, Thermo Fisher Scientific Inc., Santa Clara, CA, USA) followed by blocking using 1% BSA, PBST. In <xref ref-type="fig" rid="molecules-20-04610-f001">Figure 1</xref>C, a SARS-CoV-specific ELISA kit (EUROIMMUN AG, Lübeck, Germany) was used to measure SARS-CoV-expression of lectin-specific glyco-epitopes. Anti-glycan antibodies or biotinylated lectins were pre-titrated in 1% BSA, PBST for ELISA. The bound antibodies were revealed by an alkaline phosphatase (AP)-conjugate of goat anti-human IgG-Fc-specific antibody, and the biotinylated lectins captured were quantified by AP-streptavidin-conjugate.</p></sec></sec><sec><title>4. Conclusions</title><p>In this pilot study, we examined whether human viruses of distinct phylogenetic origins may express common carbohydrate moieties. Using carbohydrate microarrays and ELISA-based viral glycan-profiling analysis, we characterized two broadly HIV-neutralizing agents, human monoclonal antibody 2G12 and lectin GNA. Although these agents were known to target oligomannosyl antigens, they differ strikingly in the spectrum of viruses they effectively neutralize. The former is solely HIV-specific; the latter is broadly reactive with human viruses, including HIV-1, SARS-CoV, and HCMV, that are phylogenetically and pathogenically distinct. Our carbohydrate microarray analyses demonstrate the two probes differ strikingly in glycan-targeting specificities and the spectrum of glyco-epitopes they recognize. Although 2G12 is strictly specific for the high-density Man9 clusters that decorate the HIV envelope spike, GNA recognizes a number of N-glycan cryptic sugar moieties. These include oligomannoses and the previously unrecognized Tri/m-Gn-glyco-determinants. The latter appear to be the potent natural ligands of GNA. Molecular mechanisms underlying the GNA-model of “pauci reactive” glycan-binding and broad virus-neutralization warrant further investigation. Owing to the potential immunogenic activity as a plant-derived lectin, GNA is unlikely suitable for anti-virus therapy <italic>in vivo</italic>. Thus, effort must also be made to establish GNA-like potent and broadly virus-neutralizing antibodies, especially humanized or fully human mAbs that are readily applicable in the front-line biodefense against emerging viral pathogens.</p></sec></body><back><ack><title>Acknowledgments</title><p>The authors acknowledge the NIH AIDS Reagent Program for mAb 2G12 and the Kabat Collection of Carbohydrate Antigens at SRI International for a number of carbohydrate antigens that were applied in this study. This work is supported in part by NIH grant numbers R56AI108388 (D.W.), R01AI113896 (L.X.W.), SRI International’s research contract BDC12-387 (D.W.), and the National Natural Science Foundation of China project 81270665 (J.L.T.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.</p></ack><fn-group><fn><p><italic>Sample Availability</italic>: Not applicable.</p></fn></fn-group><app-group><app id="app1-molecules-20-04610"><title>Supplementary Materials</title><p>Supplementary materials can be accessed at: <uri xlink:type="simple" xlink:href="http://www.mdpi.com/1420-3049/20/03/4610/s1">http://www.mdpi.com/1420-3049/20/03/4610/s1</uri>.</p><supplementary-material content-type="local-data" id="molecules-20-04610-s001"><media xlink:href="molecules-20-04610-s001.zip"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></app></app-group><notes><title>Author Contributions</title><p>D.W. and J.L.T. conceived and designed the experiments; D.W. and J.T. performed the experiments; L.X.W. contributed key reagents/materials and edited the manuscript; D.W. analyzed the data and wrote the paper.</p></notes><glossary><title>Abbreviations</title><def-list><def-item><term>OR</term><def><p>(orosomucoid)</p></def></def-item><def-item><term>ASOR</term><def><p>(asialo-orosomucoid)</p></def></def-item><def-item><term>AGOR</term><def><p>(agalacto-orosomucoid)</p></def></def-item><def-item><term>Tri/m-II</term><def><p>(Tri-antennary and multivalent type II (Galβ1→4GlcNAc) chain epitopes)</p></def></def-item><def-item><term>Tri/m-Gn</term><def><p>(Tri-antennary or multi-valent GlcNAc-terminating epitopes)</p></def></def-item><def-item><term>GNA</term><def><p>(Galanthus nivalis agglutinin)</p></def></def-item><def-item><term>PHA-L</term><def><p>(Phaseolus vulgaris-L lectin)</p></def></def-item><def-item><term>SNA-I</term><def><p>(Sambucus nigra I agglutinin)</p></def></def-item><def-item><term>HCMV</term><def><p>(human cytomegalovirus)</p></def></def-item><def-item><term>HIV-1</term><def><p>(human immunodeficiency virus-1)</p></def></def-item><def-item><term>SARS-CoV</term><def><p>(severe acute respiratory syndrome coronavirus)</p></def></def-item></def-list></glossary><notes notes-type="COI-statement"><title>Conflicts of Interest</title><p>The authors declare no conflict of interest.</p></notes><ref-list><title>References</title><ref id="B1-molecules-20-04610"><label>1.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Calarese</surname><given-names>D.A.</given-names></name><name><surname>Scanlan</surname><given-names>C.N.</given-names></name><name><surname>Zwick</surname><given-names>M.B.</given-names></name><name><surname>Deechongkit</surname><given-names>S.</given-names></name><name><surname>Mimura</surname><given-names>Y.</given-names></name><name><surname>Kunert</surname><given-names>R.</given-names></name><name><surname>Zhu</surname><given-names>P.</given-names></name><name><surname>Wormald</surname><given-names>M.R.</given-names></name><name><surname>Stanfield</surname><given-names>R.L.</given-names></name><name><surname>Roux</surname><given-names>K.H.</given-names></name><etal></etal></person-group><article-title>Antibody domain exchange is an immunological solution to carbohydrate cluster recognition</article-title><source>Science</source><year>2003</year><volume>300</volume><fpage>2065</fpage><lpage>2071</lpage><pub-id pub-id-type="doi">10.1126/science.1083182</pub-id><pub-id pub-id-type="pmid">12829775</pub-id></element-citation></ref><ref id="B2-molecules-20-04610"><label>2.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Burton</surname><given-names>D.R.</given-names></name></person-group><article-title>Rational antibody-based HIV-1 vaccine design: Current approaches and future directions</article-title><source>Curr. Opin. Immunol.</source><year>2010</year><volume>22</volume><fpage>358</fpage><lpage>366</lpage><pub-id pub-id-type="doi">10.1016/j.coi.2010.02.012</pub-id><pub-id pub-id-type="pmid">20299194</pub-id></element-citation></ref><ref id="B3-molecules-20-04610"><label>3.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Scanlan</surname><given-names>C.N.</given-names></name><name><surname>Pantophlet</surname><given-names>R.</given-names></name><name><surname>Wormald</surname><given-names>M.R.</given-names></name><name><surname>Ollmann Saphire</surname><given-names>E.</given-names></name><name><surname>Stanfield</surname><given-names>R.</given-names></name><name><surname>Wilson</surname><given-names>I.A.</given-names></name><name><surname>Katinger</surname><given-names>H.</given-names></name><name><surname>Dwek</surname><given-names>R.A.</given-names></name><name><surname>Rudd</surname><given-names>P.M.</given-names></name><name><surname>Burton</surname><given-names>D.R.</given-names></name></person-group><article-title>The broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2G12 recognizes a cluster of alpha1-->2 mannose residues on the outer face of gp120</article-title><source>J. Virol.</source><year>2002</year><volume>76</volume><fpage>7306</fpage><lpage>7321</lpage><pub-id pub-id-type="doi">10.1128/JVI.76.14.7306-7321.2002</pub-id><pub-id pub-id-type="pmid">12072529</pub-id></element-citation></ref><ref id="B4-molecules-20-04610"><label>4.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sanders</surname><given-names>R.W.</given-names></name><name><surname>Venturi</surname><given-names>M.</given-names></name><name><surname>Schiffner</surname><given-names>L.</given-names></name><name><surname>Kalyanaraman</surname><given-names>R.</given-names></name><name><surname>Katinger</surname><given-names>H.</given-names></name><name><surname>Lloyd</surname><given-names>K.O.</given-names></name><name><surname>Kwong</surname><given-names>P.D.</given-names></name><name><surname>Moore</surname><given-names>J.P.</given-names></name></person-group><article-title>The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120</article-title><source>J. Virol.</source><year>2002</year><volume>76</volume><fpage>7293</fpage><lpage>7305</lpage><pub-id pub-id-type="doi">10.1128/JVI.76.14.7293-7305.2002</pub-id><pub-id pub-id-type="pmid">12072528</pub-id></element-citation></ref><ref id="B5-molecules-20-04610"><label>5.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Simek</surname><given-names>M.D.</given-names></name><name><surname>Priddy</surname><given-names>F.</given-names></name><name><surname>Gach</surname><given-names>J.S.</given-names></name><name><surname>Wagner</surname><given-names>D.</given-names></name><name><surname>Zwick</surname><given-names>M.B.</given-names></name><name><surname>Phogat</surname><given-names>S.K.</given-names></name><name><surname>Poignard</surname><given-names>P.</given-names></name><name><surname>Burton</surname><given-names>D.R.</given-names></name></person-group><article-title>A limited number of antibody specificities mediate broad and potent serum neutralization in selected HIV-1 infected individuals</article-title><source>PLoS Pathog.</source><year>2010</year><volume>6</volume><fpage>e1001028</fpage><pub-id pub-id-type="doi">10.1371/journal.ppat.1001028</pub-id><pub-id pub-id-type="pmid">20700449</pub-id></element-citation></ref><ref id="B6-molecules-20-04610"><label>6.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pejchal</surname><given-names>R.</given-names></name><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Stanfield</surname><given-names>R.L.</given-names></name><name><surname>Phogat</surname><given-names>S.K.</given-names></name><name><surname>Koff</surname><given-names>W.C.</given-names></name><name><surname>Poignard</surname><given-names>P.</given-names></name><name><surname>Burton</surname><given-names>D.R.</given-names></name><name><surname>Wilson</surname><given-names>I.A.</given-names></name></person-group><article-title>Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2010</year><volume>107</volume><fpage>11483</fpage><lpage>11488</lpage><pub-id pub-id-type="doi">10.1073/pnas.1004600107</pub-id><pub-id pub-id-type="pmid">20534513</pub-id></element-citation></ref><ref id="B7-molecules-20-04610"><label>7.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Falkowska</surname><given-names>E.</given-names></name><name><surname>Ramos</surname><given-names>A.</given-names></name><name><surname>Feng</surname><given-names>Y.</given-names></name><name><surname>Zhou</surname><given-names>T.</given-names></name><name><surname>Moquin</surname><given-names>S.</given-names></name><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Wu</surname><given-names>X.</given-names></name><name><surname>Seaman</surname><given-names>M.S.</given-names></name><name><surname>Wrin</surname><given-names>T.</given-names></name><name><surname>Kwong</surname><given-names>P.D.</given-names></name><etal></etal></person-group><article-title>PGV04, an HIV-1 gp120 CD4 binding site antibody, is broad and potent in neutralization but does not induce conformational changes characteristic of cd4</article-title><source>J. Virol.</source><year>2012</year><volume>86</volume><fpage>4394</fpage><lpage>4403</lpage><pub-id pub-id-type="doi">10.1128/JVI.06973-11</pub-id><pub-id pub-id-type="pmid">22345481</pub-id></element-citation></ref><ref id="B8-molecules-20-04610"><label>8.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Sok</surname><given-names>D.</given-names></name><name><surname>Nishimura</surname><given-names>Y.</given-names></name><name><surname>Donau</surname><given-names>O.</given-names></name><name><surname>Sadjadpour</surname><given-names>R.</given-names></name><name><surname>Gautam</surname><given-names>R.</given-names></name><name><surname>Shingai</surname><given-names>M.</given-names></name><name><surname>Pejchal</surname><given-names>R.</given-names></name><name><surname>Ramos</surname><given-names>A.</given-names></name><name><surname>Simek</surname><given-names>M.D.</given-names></name><etal></etal></person-group><article-title>Rapid development of glycan-specific, broad, and potent anti-HIV-1 gp120 neutralizing antibodies in an R5 SIV/HIV chimeric virus infected macaque</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2011</year><volume>108</volume><fpage>20125</fpage><lpage>20129</lpage><pub-id pub-id-type="doi">10.1073/pnas.1117531108</pub-id><pub-id pub-id-type="pmid">22123961</pub-id></element-citation></ref><ref id="B9-molecules-20-04610"><label>9.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pejchal</surname><given-names>R.</given-names></name><name><surname>Doores</surname><given-names>K.J.</given-names></name><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Khayat</surname><given-names>R.</given-names></name><name><surname>Huang</surname><given-names>P.S.</given-names></name><name><surname>Wang</surname><given-names>S.K.</given-names></name><name><surname>Stanfield</surname><given-names>R.L.</given-names></name><name><surname>Julien</surname><given-names>J.P.</given-names></name><name><surname>Ramos</surname><given-names>A.</given-names></name><name><surname>Crispin</surname><given-names>M.</given-names></name><etal></etal></person-group><article-title>A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield</article-title><source>Science</source><year>2011</year><volume>334</volume><fpage>1097</fpage><lpage>1103</lpage><pub-id pub-id-type="doi">10.1126/science.1213256</pub-id><pub-id pub-id-type="pmid">21998254</pub-id></element-citation></ref><ref id="B10-molecules-20-04610"><label>10.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Walker</surname><given-names>L.M.</given-names></name><name><surname>Huber</surname><given-names>M.</given-names></name><name><surname>Doores</surname><given-names>K.J.</given-names></name><name><surname>Falkowska</surname><given-names>E.</given-names></name><name><surname>Pejchal</surname><given-names>R.</given-names></name><name><surname>Julien</surname><given-names>J.P.</given-names></name><name><surname>Wang</surname><given-names>S.K.</given-names></name><name><surname>Ramos</surname><given-names>A.</given-names></name><name><surname>Chan-Hui</surname><given-names>P.Y.</given-names></name><name><surname>Moyle</surname><given-names>M.</given-names></name><etal></etal></person-group><article-title>Broad neutralization coverage of HIV by multiple highly potent antibodies</article-title><source>Nature</source><year>2011</year><volume>477</volume><fpage>466</fpage><lpage>470</lpage><pub-id pub-id-type="doi">10.1038/nature10373</pub-id><pub-id pub-id-type="pmid">21849977</pub-id></element-citation></ref><ref id="B11-molecules-20-04610"><label>11.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>L.X.</given-names></name></person-group><article-title>Synthetic carbohydrate antigens for HIV vaccine design</article-title><source>Curr. Opin. Chem. Biol.</source><year>2013</year><volume>17</volume><fpage>997</fpage><lpage>1005</lpage><pub-id pub-id-type="doi">10.1016/j.cbpa.2013.10.001</pub-id><pub-id pub-id-type="pmid">24466581</pub-id></element-citation></ref><ref id="B12-molecules-20-04610"><label>12.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Trkola</surname><given-names>A.</given-names></name><name><surname>Purtscher</surname><given-names>M.</given-names></name><name><surname>Muster</surname><given-names>T.</given-names></name><name><surname>Ballaun</surname><given-names>C.</given-names></name><name><surname>Buchacher</surname><given-names>A.</given-names></name><name><surname>Sullivan</surname><given-names>N.</given-names></name><name><surname>Srinivasan</surname><given-names>K.</given-names></name><name><surname>Sodroski</surname><given-names>J.</given-names></name><name><surname>Moore</surname><given-names>J.P.</given-names></name><name><surname>Katinger</surname><given-names>H.</given-names></name></person-group><article-title>Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1</article-title><source>J. Virol.</source><year>1996</year><volume>70</volume><fpage>1100</fpage><lpage>1108</lpage><pub-id pub-id-type="pmid">8551569</pub-id></element-citation></ref><ref id="B13-molecules-20-04610"><label>13.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McLellan</surname><given-names>J.S.</given-names></name><name><surname>Pancera</surname><given-names>M.</given-names></name><name><surname>Carrico</surname><given-names>C.</given-names></name><name><surname>Gorman</surname><given-names>J.</given-names></name><name><surname>Julien</surname><given-names>J.P.</given-names></name><name><surname>Khayat</surname><given-names>R.</given-names></name><name><surname>Louder</surname><given-names>R.</given-names></name><name><surname>Pejchal</surname><given-names>R.</given-names></name><name><surname>Sastry</surname><given-names>M.</given-names></name><name><surname>Dai</surname><given-names>K.</given-names></name><etal></etal></person-group><article-title>Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9</article-title><source>Nature</source><year>2011</year><volume>480</volume><fpage>336</fpage><lpage>343</lpage><pub-id pub-id-type="doi">10.1038/nature10696</pub-id><pub-id pub-id-type="pmid">22113616</pub-id></element-citation></ref><ref id="B14-molecules-20-04610"><label>14.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name><name><surname>Lu</surname><given-names>J.</given-names></name></person-group><article-title>Glycan arrays lead to the discovery of autoimmunogenic activity of SARS-CoV</article-title><source>Physiol. Genomics</source><year>2004</year><volume>18</volume><fpage>245</fpage><lpage>248</lpage><pub-id pub-id-type="doi">10.1152/physiolgenomics.00102.2004</pub-id><pub-id pub-id-type="pmid">15161967</pub-id></element-citation></ref><ref id="B15-molecules-20-04610"><label>15.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Eickmann</surname><given-names>M.</given-names></name><name><surname>Becker</surname><given-names>S.</given-names></name><name><surname>Klenk</surname><given-names>H.D.</given-names></name><name><surname>Doerr</surname><given-names>H.W.</given-names></name><name><surname>Stadler</surname><given-names>K.</given-names></name><name><surname>Censini</surname><given-names>S.</given-names></name><name><surname>Guidotti</surname><given-names>S.</given-names></name><name><surname>Masignani</surname><given-names>V.</given-names></name><name><surname>Scarselli</surname><given-names>M.</given-names></name><name><surname>Mora</surname><given-names>M.</given-names></name><etal></etal></person-group><article-title>Phylogeny of the SARS coronavirus</article-title><source>Science</source><year>2003</year><volume>302</volume><fpage>1504</fpage><lpage>1505</lpage><pub-id pub-id-type="doi">10.1126/science.302.5650.1504b</pub-id><pub-id pub-id-type="pmid">14645828</pub-id></element-citation></ref><ref id="B16-molecules-20-04610"><label>16.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marra</surname><given-names>M.A.</given-names></name><name><surname>Jones</surname><given-names>S.J.</given-names></name><name><surname>Astell</surname><given-names>C.R.</given-names></name><name><surname>Holt</surname><given-names>R.A.</given-names></name><name><surname>Brooks-Wilson</surname><given-names>A.</given-names></name><name><surname>Butterfield</surname><given-names>Y.S.</given-names></name><name><surname>Khattra</surname><given-names>J.</given-names></name><name><surname>Asano</surname><given-names>J.K.</given-names></name><name><surname>Barber</surname><given-names>S.A.</given-names></name><name><surname>Chan</surname><given-names>S.Y.</given-names></name><etal></etal></person-group><article-title>The genome sequence of the SARS-associated coronavirus</article-title><source>Science</source><year>2003</year><volume>300</volume><fpage>1399</fpage><lpage>1404</lpage><pub-id pub-id-type="doi">10.1126/science.1085953</pub-id><pub-id pub-id-type="pmid">12730501</pub-id></element-citation></ref><ref id="B17-molecules-20-04610"><label>17.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rota</surname><given-names>P.A.</given-names></name><name><surname>Oberste</surname><given-names>M.S.</given-names></name><name><surname>Monroe</surname><given-names>S.S.</given-names></name><name><surname>Nix</surname><given-names>W.A.</given-names></name><name><surname>Campagnoli</surname><given-names>R.</given-names></name><name><surname>Icenogle</surname><given-names>J.P.</given-names></name><name><surname>Penaranda</surname><given-names>S.</given-names></name><name><surname>Bankamp</surname><given-names>B.</given-names></name><name><surname>Maher</surname><given-names>K.</given-names></name><name><surname>Chen</surname><given-names>M.H.</given-names></name><etal></etal></person-group><article-title>Characterization of a novel coronavirus associated with severe acute respiratory syndrome</article-title><source>Science</source><year>2003</year><volume>300</volume><fpage>1394</fpage><lpage>1399</lpage><pub-id pub-id-type="doi">10.1126/science.1085952</pub-id><pub-id pub-id-type="pmid">12730500</pub-id></element-citation></ref><ref id="B18-molecules-20-04610"><label>18.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Xiao</surname><given-names>X.</given-names></name><name><surname>Chakraborti</surname><given-names>S.</given-names></name><name><surname>Dimitrov</surname><given-names>A.S.</given-names></name><name><surname>Gramatikoff</surname><given-names>K.</given-names></name><name><surname>Dimitrov</surname><given-names>D.S.</given-names></name></person-group><article-title>The SARS-COV S glycoprotein: Expression and functional characterization</article-title><source>Biochem. Biophys. Res. Commun.</source><year>2003</year><volume>312</volume><fpage>1159</fpage><lpage>1164</lpage><pub-id pub-id-type="doi">10.1016/j.bbrc.2003.11.054</pub-id><pub-id pub-id-type="pmid">14651994</pub-id></element-citation></ref><ref id="B19-molecules-20-04610"><label>19.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ritchie</surname><given-names>G.</given-names></name><name><surname>Harvey</surname><given-names>D.J.</given-names></name><name><surname>Feldmann</surname><given-names>F.</given-names></name><name><surname>Stroeher</surname><given-names>U.</given-names></name><name><surname>Feldmann</surname><given-names>H.</given-names></name><name><surname>Royle</surname><given-names>L.</given-names></name><name><surname>Dwek</surname><given-names>R.A.</given-names></name><name><surname>Rudd</surname><given-names>P.M.</given-names></name></person-group><article-title>Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoprotein</article-title><source>Virology</source><year>2010</year><volume>399</volume><fpage>257</fpage><lpage>269</lpage><pub-id pub-id-type="doi">10.1016/j.virol.2009.12.020</pub-id><pub-id pub-id-type="pmid">20129637</pub-id></element-citation></ref><ref id="B20-molecules-20-04610"><label>20.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balzarini</surname><given-names>J.</given-names></name><name><surname>Schols</surname><given-names>D.</given-names></name><name><surname>Neyts</surname><given-names>J.</given-names></name><name><surname>Van Damme</surname><given-names>E.</given-names></name><name><surname>Peumans</surname><given-names>W.</given-names></name><name><surname>de Clercq</surname><given-names>E.</given-names></name></person-group><article-title>Alpha-(1–3)- and alpha-(1–6)-<sc>d</sc>-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections <italic>in vitro</italic></article-title><source>Antimicrob. Agents Chemother.</source><year>1991</year><volume>35</volume><fpage>410</fpage><lpage>416</lpage><pub-id pub-id-type="doi">10.1128/AAC.35.3.410</pub-id><pub-id pub-id-type="pmid">1645507</pub-id></element-citation></ref><ref id="B21-molecules-20-04610"><label>21.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balzarini</surname><given-names>J.</given-names></name><name><surname>van Laethem</surname><given-names>K.</given-names></name><name><surname>Hatse</surname><given-names>S.</given-names></name><name><surname>Froeyen</surname><given-names>M.</given-names></name><name><surname>van Damme</surname><given-names>E.</given-names></name><name><surname>Bolmstedt</surname><given-names>A.</given-names></name><name><surname>Peumans</surname><given-names>W.</given-names></name><name><surname>de Clercq</surname><given-names>E.</given-names></name><name><surname>Schols</surname><given-names>D.</given-names></name></person-group><article-title>Marked depletion of glycosylation sites in HIV-1 gp120 under selection pressure by the mannose-specific plant lectins of Hippeastrum hybrid and Galanthus nivalis</article-title><source>Mol. Pharmacol.</source><year>2005</year><volume>67</volume><fpage>1556</fpage><lpage>1565</lpage><pub-id pub-id-type="doi">10.1124/mol.104.005082</pub-id><pub-id pub-id-type="pmid">15718224</pub-id></element-citation></ref><ref id="B22-molecules-20-04610"><label>22.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balzarini</surname><given-names>J.</given-names></name><name><surname>van Laethem</surname><given-names>K.</given-names></name><name><surname>Hatse</surname><given-names>S.</given-names></name><name><surname>Vermeire</surname><given-names>K.</given-names></name><name><surname>de Clercq</surname><given-names>E.</given-names></name><name><surname>Peumans</surname><given-names>W.</given-names></name><name><surname>van Damme</surname><given-names>E.</given-names></name><name><surname>Vandamme</surname><given-names>A.M.</given-names></name><name><surname>Bolmstedt</surname><given-names>A.</given-names></name><name><surname>Schols</surname><given-names>D.</given-names></name></person-group><article-title>Profile of resistance of human immunodeficiency virus to mannose-specific plant lectins</article-title><source>J. Virol.</source><year>2004</year><volume>78</volume><fpage>10617</fpage><lpage>10627</lpage><pub-id pub-id-type="doi">10.1128/JVI.78.19.10617-10627.2004</pub-id><pub-id pub-id-type="pmid">15367629</pub-id></element-citation></ref><ref id="B23-molecules-20-04610"><label>23.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balzarini</surname><given-names>J.</given-names></name><name><surname>Hatse</surname><given-names>S.</given-names></name><name><surname>Vermeire</surname><given-names>K.</given-names></name><name><surname>Princen</surname><given-names>K.</given-names></name><name><surname>Aquaro</surname><given-names>S.</given-names></name><name><surname>Perno</surname><given-names>C.F.</given-names></name><name><surname>de Clercq</surname><given-names>E.</given-names></name><name><surname>Egberink</surname><given-names>H.</given-names></name><name><surname>Vanden Mooter</surname><given-names>G.</given-names></name><name><surname>Peumans</surname><given-names>W.</given-names></name><etal></etal></person-group><article-title>Mannose-specific plant lectins from the amaryllidaceae family qualify as efficient microbicides for prevention of human immunodeficiency virus infection</article-title><source>Antimicrob. Agents Chemother.</source><year>2004</year><volume>48</volume><fpage>3858</fpage><lpage>3870</lpage><pub-id pub-id-type="doi">10.1128/AAC.48.10.3858-3870.2004</pub-id><pub-id pub-id-type="pmid">15388446</pub-id></element-citation></ref><ref id="B24-molecules-20-04610"><label>24.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Keyaerts</surname><given-names>E.</given-names></name><name><surname>Vijgen</surname><given-names>L.</given-names></name><name><surname>Pannecouque</surname><given-names>C.</given-names></name><name><surname>van Damme</surname><given-names>E.</given-names></name><name><surname>Peumans</surname><given-names>W.</given-names></name><name><surname>Egberink</surname><given-names>H.</given-names></name><name><surname>Balzarini</surname><given-names>J.</given-names></name><name><surname>van Ranst</surname><given-names>M.</given-names></name></person-group><article-title>Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle</article-title><source>Antivir. Res.</source><year>2007</year><volume>75</volume><fpage>179</fpage><lpage>187</lpage><pub-id pub-id-type="doi">10.1016/j.antiviral.2007.03.003</pub-id><pub-id pub-id-type="pmid">17428553</pub-id></element-citation></ref><ref id="B25-molecules-20-04610"><label>25.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yang</surname><given-names>Q.</given-names></name><name><surname>Li</surname><given-names>C.</given-names></name><name><surname>Wei</surname><given-names>Y.</given-names></name><name><surname>Huang</surname><given-names>W.</given-names></name><name><surname>Wang</surname><given-names>L.X.</given-names></name></person-group><article-title>Expression, glycoform characterization, and antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc</article-title><source>Bioconjugate Chem.</source><year>2010</year><volume>21</volume><fpage>875</fpage><lpage>883</lpage><pub-id pub-id-type="doi">10.1021/bc9004238</pub-id></element-citation></ref><ref id="B26-molecules-20-04610"><label>26.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Scanlan</surname><given-names>C.N.</given-names></name><name><surname>Ritchie</surname><given-names>G.E.</given-names></name><name><surname>Baruah</surname><given-names>K.</given-names></name><name><surname>Crispin</surname><given-names>M.</given-names></name><name><surname>Harvey</surname><given-names>D.J.</given-names></name><name><surname>Singer</surname><given-names>B.B.</given-names></name><name><surname>Lucka</surname><given-names>L.</given-names></name><name><surname>Wormald</surname><given-names>M.R.</given-names></name><name><surname>Wentworth</surname><given-names>P.</given-names><suffix>Jr.</suffix></name><name><surname>Zitzmann</surname><given-names>N.</given-names></name><etal></etal></person-group><article-title>Inhibition of mammalian glycan biosynthesis produces non-self antigens for a broadly neutralising, HIV-1 specific antibody</article-title><source>J. Mol. Biol.</source><year>2007</year><volume>372</volume><fpage>16</fpage><lpage>22</lpage><pub-id pub-id-type="doi">10.1016/j.jmb.2007.06.027</pub-id><pub-id pub-id-type="pmid">17631311</pub-id></element-citation></ref><ref id="B27-molecules-20-04610"><label>27.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Doores</surname><given-names>K.J.</given-names></name><name><surname>Bonomelli</surname><given-names>C.</given-names></name><name><surname>Harvey</surname><given-names>D.J.</given-names></name><name><surname>Vasiljevic</surname><given-names>S.</given-names></name><name><surname>Dwek</surname><given-names>R.A.</given-names></name><name><surname>Burton</surname><given-names>D.R.</given-names></name><name><surname>Crispin</surname><given-names>M.</given-names></name><name><surname>Scanlan</surname><given-names>C.N.</given-names></name></person-group><article-title>Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>2010</year><volume>107</volume><fpage>13800</fpage><lpage>13805</lpage><pub-id pub-id-type="doi">10.1073/pnas.1006498107</pub-id><pub-id pub-id-type="pmid">20643940</pub-id></element-citation></ref><ref id="B28-molecules-20-04610"><label>28.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rambaruth</surname><given-names>N.D.</given-names></name><name><surname>Greenwell</surname><given-names>P.</given-names></name><name><surname>Dwek</surname><given-names>M.V.</given-names></name></person-group><article-title>The lectin helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer</article-title><source>Glycobiology</source><year>2012</year><volume>22</volume><fpage>839</fpage><lpage>848</lpage><pub-id pub-id-type="doi">10.1093/glycob/cws051</pub-id><pub-id pub-id-type="pmid">22322011</pub-id></element-citation></ref><ref id="B29-molecules-20-04610"><label>29.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tempel</surname><given-names>W.</given-names></name><name><surname>Tschampel</surname><given-names>S.</given-names></name><name><surname>Woods</surname><given-names>R.J.</given-names></name></person-group><article-title>The xenograft antigen bound to <italic>Griffonia simplicifolia</italic> lectin 1-B<sub>4</sub>. X-ray crystal structure of the complex and molecular dynamics characterization of the binding site</article-title><source>J. Biol. Chem.</source><year>2002</year><volume>277</volume><fpage>6615</fpage><lpage>6621</lpage><pub-id pub-id-type="doi">10.1074/jbc.M109919200</pub-id><pub-id pub-id-type="pmid">11714721</pub-id></element-citation></ref><ref id="B30-molecules-20-04610"><label>30.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Galili</surname><given-names>U.</given-names></name><name><surname>Clark</surname><given-names>M.R.</given-names></name><name><surname>Shohet</surname><given-names>S.B.</given-names></name><name><surname>Buehler</surname><given-names>J.</given-names></name><name><surname>Macher</surname><given-names>B.A.</given-names></name></person-group><article-title>Evolutionary relationship between the natural anti-Gal antibody and the Gal alpha 1----3Gal epitope in primates</article-title><source>Proc. Natl. Acad. Sci. USA</source><year>1987</year><volume>84</volume><fpage>1369</fpage><lpage>1373</lpage><pub-id pub-id-type="doi">10.1073/pnas.84.5.1369</pub-id><pub-id pub-id-type="pmid">2434954</pub-id></element-citation></ref><ref id="B31-molecules-20-04610"><label>31.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name><name><surname>Bhat</surname><given-names>R.</given-names></name><name><surname>Sobel</surname><given-names>R.A.</given-names></name><name><surname>Huang</surname><given-names>W.</given-names></name><name><surname>Wang</surname><given-names>L.X.</given-names></name><name><surname>Olsson</surname><given-names>T.</given-names></name><name><surname>Steinman</surname><given-names>L.</given-names></name></person-group><article-title>Uncovering cryptic glycan markers in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE)</article-title><source>Drug Dev. Res.</source><year>2014</year><volume>75</volume><fpage>172</fpage><lpage>188</lpage><pub-id pub-id-type="doi">10.1002/ddr.21157</pub-id><pub-id pub-id-type="pmid">24648292</pub-id></element-citation></ref><ref id="B32-molecules-20-04610"><label>32.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Benko</surname><given-names>D.M.</given-names></name><name><surname>Gibson</surname><given-names>W.</given-names></name></person-group><article-title>Primate cytomegalovirus glycoproteins: Lectin-binding properties and sensitivities to glycosidases</article-title><source>J. Virol.</source><year>1986</year><volume>59</volume><fpage>703</fpage><lpage>713</lpage><pub-id pub-id-type="pmid">3016332</pub-id></element-citation></ref><ref id="B33-molecules-20-04610"><label>33.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Weekes</surname><given-names>M.P.</given-names></name><name><surname>Tomasec</surname><given-names>P.</given-names></name><name><surname>Huttlin</surname><given-names>E.L.</given-names></name><name><surname>Fielding</surname><given-names>C.A.</given-names></name><name><surname>Nusinow</surname><given-names>D.</given-names></name><name><surname>Stanton</surname><given-names>R.J.</given-names></name><name><surname>Wang</surname><given-names>E.C.</given-names></name><name><surname>Aicheler</surname><given-names>R.</given-names></name><name><surname>Murrell</surname><given-names>I.</given-names></name><name><surname>Wilkinson</surname><given-names>G.W.</given-names></name><etal></etal></person-group><article-title>Quantitative temporal viromics: An approach to investigate host-pathogen interaction</article-title><source>Cell</source><year>2014</year><volume>157</volume><fpage>1460</fpage><lpage>1472</lpage><pub-id pub-id-type="doi">10.1016/j.cell.2014.04.028</pub-id><pub-id pub-id-type="pmid">24906157</pub-id></element-citation></ref><ref id="B34-molecules-20-04610"><label>34.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name><name><surname>Dafik</surname><given-names>L.</given-names></name><name><surname>Nolley</surname><given-names>R.</given-names></name><name><surname>Huang</surname><given-names>W.</given-names></name><name><surname>Wolfinger</surname><given-names>R.D.</given-names></name><name><surname>Wang</surname><given-names>L.X.</given-names></name><name><surname>Peehl</surname><given-names>D.M.</given-names></name></person-group><article-title>Anti-oligomannose antibodies as potential serum biomarkers of aggressive prostate cancer</article-title><source>Drug Dev. Res.</source><year>2013</year><volume>74</volume><fpage>65</fpage><lpage>80</lpage><pub-id pub-id-type="doi">10.1002/ddr.21063</pub-id><pub-id pub-id-type="pmid">25152555</pub-id></element-citation></ref><ref id="B35-molecules-20-04610"><label>35.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name></person-group><article-title>N-glycan cryptic antigens as active immunological targets in prostate cancer patients</article-title><source>J. Proteomics Bioinform.</source><year>2012</year><volume>5</volume><fpage>090</fpage><lpage>095</lpage><pub-id pub-id-type="pmid">25284963</pub-id></element-citation></ref><ref id="B36-molecules-20-04610"><label>36.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name></person-group><article-title>Carbohydrate antigen microarrays</article-title><source>Methods Mol. Biol.</source><year>2012</year><volume>808</volume><fpage>241</fpage><lpage>249</lpage><pub-id pub-id-type="pmid">22057530</pub-id></element-citation></ref><ref id="B37-molecules-20-04610"><label>37.</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>D.</given-names></name><name><surname>Liu</surname><given-names>S.</given-names></name><name><surname>Trummer</surname><given-names>B.J.</given-names></name><name><surname>Deng</surname><given-names>C.</given-names></name><name><surname>Wang</surname><given-names>A.</given-names></name></person-group><article-title>Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells</article-title><source>Nat. Biotechnol.</source><year>2002</year><volume>20</volume><fpage>275</fpage><lpage>281</lpage><pub-id pub-id-type="doi">10.1038/nbt0302-275</pub-id><pub-id pub-id-type="pmid">11875429</pub-id></element-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001300 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001300 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.33. |  |