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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice</title><author><name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au2"><institution>Anhui Medical University</institution><addr-line>Hefei, China</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huang, Li" sort="Huang, Li" uniqKey="Huang L" first="Li" last="Huang">Li Huang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name><affiliation><nlm:aff id="au2"><institution>Anhui Medical University</institution><addr-line>Hefei, China</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20406307</idno><idno type="pmc">2878469</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878469</idno><idno type="RBID">PMC:2878469</idno><idno type="doi">10.1111/j.1365-2567.2010.03231.x</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000C53</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice</title><author><name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au2"><institution>Anhui Medical University</institution><addr-line>Hefei, China</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Huang, Li" sort="Huang, Li" uniqKey="Huang L" first="Li" last="Huang">Li Huang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name><affiliation><nlm:aff id="au2"><institution>Anhui Medical University</institution><addr-line>Hefei, China</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="au1"><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">Immunology</title><idno type="ISSN">0019-2805</idno><idno type="eISSN">1365-2567</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Nasal administration has emerged as a promising and attractive route for vaccination, especially for the prophylaxis of respiratory diseases. Our previous studies have shown that severe acute respiratory syndrome coronavirus (SARS-CoV) virus-like particles (VLPs) can be assembled using a recombinant baculovirus (rBV) expression system and such VLPs induce specific humoral and cellular immune responses in mice after subcutaneous injection. Here, we investigated mucosal immune responses to SARS-CoV VLPs in a mouse model. Mice were immunized in parallel, intraperitoneally or intranasally, with VLPs alone or with VLPs plus cytosine–phosphate–guanosine (CpG). Immune responses, including the production of SARS-CoV-specific serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), were determined in mucosal secretions and tissues. Both immunizations induced SARS-CoV-specific IgG, although the levels of IgG in groups immunized via the intraperitoneal (i.p.) route were higher. sIgA was detected in saliva in groups immunized intranasally but not in groups immunized intraperitoneally. CpG had an adjuvant effect on IgA production in genital tract washes when administered intranasally but only affected IgA production in faeces samples when administered intraperitoneally. In addition, IgA was also detected in mucosal tissues from the lung and intestine, while CpG induced an increased level of IgA in the intestine. Most importantly, neutralization antibodies were detected in sera after i.p. and intranasal (i.n.) immunizations. Secretions in genital tract washes from the i.n. group also showed neutralization activity. Furthermore, VLPs that were administered intraperitoneally elicited cellular immune responses as demonstrated by enzyme-linked immunospot (ELISPOT) assay analyses. In summary, our study indicates that mucosal immunization with rBV SARS-CoV VLPs represent an effective means for eliciting protective systemic and mucosal immune responses against SARS-CoV, providing important information for vaccine design.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Immunology</journal-id><journal-id journal-id-type="publisher-id">imm</journal-id><journal-title-group><journal-title>Immunology</journal-title></journal-title-group><issn pub-type="ppub">0019-2805</issn><issn pub-type="epub">1365-2567</issn><publisher><publisher-name>Blackwell Science Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20406307</article-id><article-id pub-id-type="pmc">2878469</article-id><article-id pub-id-type="doi">10.1111/j.1365-2567.2010.03231.x</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject></subj-group></article-categories><title-group><article-title>Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Baojing</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="aff" rid="au2">2</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Li</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Bao</given-names></name><xref ref-type="aff" rid="au2">2</xref></contrib><contrib contrib-type="author"><name><surname>Zheng</surname><given-names>Zhenhua</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Ze</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Jianjun</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Qinxue</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hanzhong</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><aff id="au1"><label>1</label><institution>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science</institution><addr-line>Wuhan</addr-line></aff><aff id="au2"><label>2</label><institution>Anhui Medical University</institution><addr-line>Hefei, China</addr-line></aff></contrib-group><author-notes><corresp id="cor1">Dr H. Wang or Dr Q. Hu, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China. Emails: <email>wanghz@wh.iov.cn</email>; or <email>huqinxue@hotmail.com</email> Senior author: Dr B. Lu, email:<email>lubaojing@163.com</email></corresp></author-notes><pub-date pub-type="ppub"><month>6</month><year>2010</year></pub-date><volume>130</volume><issue>2</issue><fpage>254</fpage><lpage>261</lpage><history><date date-type="received"><day>14</day><month>8</month><year>2009</year></date><date date-type="rev-recd"><day>26</day><month>12</month><year>2009</year></date><date date-type="accepted"><day>09</day><month>12</month><year>2009</year></date></history><permissions><copyright-statement>Journal compilation © 2010 Blackwell Publishing Ltd</copyright-statement></permissions><abstract><p>Nasal administration has emerged as a promising and attractive route for vaccination, especially for the prophylaxis of respiratory diseases. Our previous studies have shown that severe acute respiratory syndrome coronavirus (SARS-CoV) virus-like particles (VLPs) can be assembled using a recombinant baculovirus (rBV) expression system and such VLPs induce specific humoral and cellular immune responses in mice after subcutaneous injection. Here, we investigated mucosal immune responses to SARS-CoV VLPs in a mouse model. Mice were immunized in parallel, intraperitoneally or intranasally, with VLPs alone or with VLPs plus cytosine–phosphate–guanosine (CpG). Immune responses, including the production of SARS-CoV-specific serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), were determined in mucosal secretions and tissues. Both immunizations induced SARS-CoV-specific IgG, although the levels of IgG in groups immunized via the intraperitoneal (i.p.) route were higher. sIgA was detected in saliva in groups immunized intranasally but not in groups immunized intraperitoneally. CpG had an adjuvant effect on IgA production in genital tract washes when administered intranasally but only affected IgA production in faeces samples when administered intraperitoneally. In addition, IgA was also detected in mucosal tissues from the lung and intestine, while CpG induced an increased level of IgA in the intestine. Most importantly, neutralization antibodies were detected in sera after i.p. and intranasal (i.n.) immunizations. Secretions in genital tract washes from the i.n. group also showed neutralization activity. Furthermore, VLPs that were administered intraperitoneally elicited cellular immune responses as demonstrated by enzyme-linked immunospot (ELISPOT) assay analyses. In summary, our study indicates that mucosal immunization with rBV SARS-CoV VLPs represent an effective means for eliciting protective systemic and mucosal immune responses against SARS-CoV, providing important information for vaccine design.</p></abstract><kwd-group><kwd>cytosine–phosphate–guanosine (CpG)</kwd><kwd>mucosal immunization</kwd><kwd>severe acute respiratory syndrome coronavirus (SARS-CoV)</kwd><kwd>virus-like particles</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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