新型冠状病毒SARS-CoV-2的实验室检测技术
Identifieur interne : 000C31 ( Pmc/Corpus ); précédent : 000C30; suivant : 000C32新型冠状病毒SARS-CoV-2的实验室检测技术
Auteurs :Source :
- Journal of Southern Medical University [ 1673-4254 ] ; 2020.
Abstract
2019年12月起由新型冠状病毒(SARS-CoV-2)导致的新型冠状病毒肺炎对全球公共卫生造成了重大和急迫威胁。该病毒传染性强，主要通过飞沫和接触传播，感染者出现肺炎几率较高，少数患者会出现急性呼吸窘迫综合征合并严重呼吸道并发症，甚至导致死亡。因此，选择合适的检测技术和方法对病原学做出准确、快速的鉴定，对提高疾病的诊断和治疗效率，遏制传染病爆发流行起关键作用。本文将对该病毒实验室检测技术，包括病毒分离培养、实时荧光RT-PCR、基因测序、血清学抗体检测及基于CRISPR/Cas13系统的基因编辑技术等进行概述，以期为临床医生及科研工作者提供更好地防治策略和研究思路。
Url:
DOI: 10.12122/j.issn.1673-4254.2020.02.04
PubMed: NONE
PubMed Central: 7086127
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PMC:7086127Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">新型冠状病毒SARS-CoV-2的实验室检测技术</title></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7086127</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086127</idno><idno type="RBID">PMC:7086127</idno><idno type="doi">10.12122/j.issn.1673-4254.2020.02.04</idno><idno type="pmid">NONE</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000C31</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C31</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">新型冠状病毒SARS-CoV-2的实验室检测技术</title></analytic><series><title level="j">Journal of Southern Medical University</title><idno type="ISSN">1673-4254</idno><idno type="eISSN">2663-0842</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>2019年12月起由新型冠状病毒(SARS-CoV-2)导致的新型冠状病毒肺炎对全球公共卫生造成了重大和急迫威胁。该病毒传染性强，主要通过飞沫和接触传播，感染者出现肺炎几率较高，少数患者会出现急性呼吸窘迫综合征合并严重呼吸道并发症，甚至导致死亡。因此，选择合适的检测技术和方法对病原学做出准确、快速的鉴定，对提高疾病的诊断和治疗效率，遏制传染病爆发流行起关键作用。本文将对该病毒实验室检测技术，包括病毒分离培养、实时荧光RT-PCR、基因测序、血清学抗体检测及基于CRISPR/Cas13系统的基因编辑技术等进行概述，以期为临床医生及科研工作者提供更好地防治策略和研究思路。</p></div></front></TEI><pmc article-type="research-article" xml:lang="zh"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Nan Fang Yi Ke Da Xue Xue Bao</journal-id><journal-id journal-id-type="iso-abbrev">Nan Fang Yi Ke Da Xue Xue Bao</journal-id><journal-id journal-id-type="publisher-id">NFYKDXXB</journal-id><journal-title-group><journal-title>Journal of Southern Medical University</journal-title></journal-title-group><issn pub-type="ppub">1673-4254</issn><issn pub-type="epub">2663-0842</issn><publisher><publisher-name>南方医科大学学报编辑部</publisher-name><publisher-loc>中国广东</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7086127</article-id><article-id pub-id-type="publisher-id">nfykdxxb-40-2-164</article-id><article-id pub-id-type="doi">10.12122/j.issn.1673-4254.2020.02.04</article-id><article-categories><subj-group subj-group-type="heading"><subject>新型冠状病毒肺炎专题</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Covid-19</subject></subj-group></article-categories><title-group><article-title>新型冠状病毒SARS-CoV-2的实验室检测技术</article-title><trans-title-group xml:lang="en"><trans-title xml:lang="en">Laboratory testing techniques for SARS-CoV-2</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>邱</surname><given-names>峰</given-names></name><name xml:lang="en"><surname>QIU</surname><given-names>Feng</given-names></name></name-alternatives><email>QFSFL@126.com</email><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="bio" rid="d35e51">*</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>王</surname><given-names>慧君</given-names></name><name xml:lang="en"><surname>WANG</surname><given-names>Huijun</given-names></name></name-alternatives><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>张</surname><given-names>子康</given-names></name><name xml:lang="en"><surname>ZHANG</surname><given-names>Zikang</given-names></name></name-alternatives><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>操</surname><given-names>龙斌</given-names></name><name xml:lang="en"><surname>CAO</surname><given-names>Longbin</given-names></name></name-alternatives><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>王</surname><given-names>陈龙</given-names></name><name xml:lang="en"><surname>WANG</surname><given-names>Chenlong</given-names></name></name-alternatives><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>吴</surname><given-names>静标</given-names></name><name xml:lang="en"><surname>WU</surname><given-names>Jingbiao</given-names></name></name-alternatives><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name-alternatives><name name-style="eastern"><surname>杜</surname><given-names>庆锋</given-names></name><name xml:lang="en"><surname>DU</surname><given-names>Qingfeng</given-names></name></name-alternatives><email>dqf@vip.163.com</email><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>南方医科大学南海医院医学检验科, 广东 佛山 528244</addr-line><addr-line xml:lang="en">Department of Laboratory Medicine, Nanhai Hospital, Southern Medical University, Foshan 528244, China</addr-line></aff><aff id="aff2"><label>2</label><addr-line>南方医科大学南海医院全科医学中心，广东 佛山 528244</addr-line><addr-line xml:lang="en">General Practice Center, Nanhai Hospital, Southern Medical University, Foshan 528244, China</addr-line></aff><aff id="aff3"><label>3</label><addr-line> 广东省医疗器械质量监督检验所，广东 广州 510663</addr-line><addr-line xml:lang="en">Guangdong Medical Devices Quality Surveillance and Test Institute, Guangzhou 510663, China</addr-line></aff><author-notes><corresp id="cor1">杜庆锋，博士，教授，E-mail: <email>dqf@vip.163.com</email></corresp></author-notes><pub-date pub-type="ppub"><day>20</day><month>2</month><year>2020</year></pub-date><volume>40</volume><issue>2</issue><fpage>164</fpage><lpage>167</lpage><history><date date-type="received"><day>17</day><month>2</month><year>2020</year></date></history><permissions><copyright-statement>版权所有©《南方医科大学学报》编辑部2020</copyright-statement><copyright-statement xml:lang="en">Copyright ©2020 Journal of Southern Medical University. All rights reserved.</copyright-statement><copyright-year>2020</copyright-year></permissions><abstract><p>2019年12月起由新型冠状病毒(SARS-CoV-2)导致的新型冠状病毒肺炎对全球公共卫生造成了重大和急迫威胁。该病毒传染性强，主要通过飞沫和接触传播，感染者出现肺炎几率较高，少数患者会出现急性呼吸窘迫综合征合并严重呼吸道并发症，甚至导致死亡。因此，选择合适的检测技术和方法对病原学做出准确、快速的鉴定，对提高疾病的诊断和治疗效率，遏制传染病爆发流行起关键作用。本文将对该病毒实验室检测技术，包括病毒分离培养、实时荧光RT-PCR、基因测序、血清学抗体检测及基于CRISPR/Cas13系统的基因编辑技术等进行概述，以期为临床医生及科研工作者提供更好地防治策略和研究思路。</p></abstract><trans-abstract xml:lang="en"><p>The ongoing epidemic of caused by the coronavirus SARS-CoV-2 starting in December 2019 poses a serious public health threat globally. The virus is highly infectious and transmitted mainly through droplets and contacts, and is associated with a high risk of pneumonia. A small number of patients may present with acute respiratory distress syndrome with severe respiratory complications, which can lead even to death. The selection of appropriate detection techniques and methods for accurate and rapid identification of pathogens therefore plays a key role in improving the diagnosis and treatment of the patients and containing the outbreak. In this review, the authors gives an overview of the virus laboratory detection technology, including virus isolation and culture, real-time fluorescent PCR, gene sequencing, serological antibody detection, and the gene editing technology based on CRISPR/Cas13 system. These techniques are expected to provide valuable assistance in controlling the epidemic and new ideas for future researches.</p></trans-abstract><kwd-group kwd-group-type="author-created"><kwd>新型冠状病毒</kwd><kwd>SARS-CoV-2</kwd><kwd>PCR</kwd><kwd>基因编辑</kwd></kwd-group><kwd-group kwd-group-type="author-created" xml:lang="en"><kwd>COVID-19</kwd><kwd>SARS-CoV-2</kwd><kwd>PCR</kwd><kwd>gene editing</kwd></kwd-group><funding-group><award-group><funding-source>佛山市十三五医学重点专科建设项目</funding-source><award-id>FSZDZK135045</award-id></award-group><award-group><funding-source>广东省科技计划项目</funding-source><award-id>2019B020208009</award-id></award-group><award-group><funding-source>广东省自然科学基金</funding-source><award-id>2018A0303130346</award-id></award-group><funding-statement>佛山市十三五医学重点专科建设项目(FSZDZK135045)；广东省科技计划项目(2019B020208009)；广东省自然科学基金(2018A0303130346)</funding-statement></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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