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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein</title><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name></author><author><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name></author><author><name sortKey="Salomoni, Aurelia" sort="Salomoni, Aurelia" uniqKey="Salomoni A" first="Aurelia" last="Salomoni">Aurelia Salomoni</name></author><author><name sortKey="Durousseau, Cecile" sort="Durousseau, Cecile" uniqKey="Durousseau C" first="Cécile" last="Durousseau">Cécile Durousseau</name></author><author><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></author><author><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name></author><author><name sortKey="Dobbe, Jessika C" sort="Dobbe, Jessika C" uniqKey="Dobbe J" first="Jessika C." last="Dobbe">Jessika C. Dobbe</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12925794</idno><idno type="pmc">7161644</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161644</idno><idno type="RBID">PMC:7161644</idno><idno type="doi">10.1107/S0907444903016779</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000B47</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B47</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein</title><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name></author><author><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name></author><author><name sortKey="Salomoni, Aurelia" sort="Salomoni, Aurelia" uniqKey="Salomoni A" first="Aurelia" last="Salomoni">Aurelia Salomoni</name></author><author><name sortKey="Durousseau, Cecile" sort="Durousseau, Cecile" uniqKey="Durousseau C" first="Cécile" last="Durousseau">Cécile Durousseau</name></author><author><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></author><author><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name></author><author><name sortKey="Dobbe, Jessika C" sort="Dobbe, Jessika C" uniqKey="Dobbe J" first="Jessika C." last="Dobbe">Jessika C. Dobbe</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name></author></analytic><series><title level="j">Acta Crystallographica Section D: Biological Crystallography</title><idno type="ISSN">0907-4449</idno><idno type="eISSN">1399-0047</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive‐stranded RNA virus (SARS‐CoV) belonging to the <italic>Coronaviridae</italic> family and its genome differs substantially from those of other known coronaviruses. SARS‐CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS‐CoV whole‐genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20–30 mature proteins. The functions of many of these polypeptides, including the Nsp9–Nsp10 replicase‐cleavage products, are still unknown. Here, the cloning, <italic>Escherichia coli</italic> expression, purification and crystallization of the SARS‐CoV Nsp9 protein, the first SARS‐CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 Å resolution and belong to space group <italic>P</italic>6<sub>1/5</sub>22, with unit‐cell parameters <italic>a</italic> = <italic>b</italic> = 89.7, <italic>c</italic> = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (<italic>V</italic><sub>M</sub> = 3.1 Å<sup>3</sup> Da<sup>−1</sup>).</p></div></front></TEI><pmc article-type="other"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Crystallogr D Biol Crystallogr</journal-id><journal-id journal-id-type="iso-abbrev">Acta Crystallogr. D Biol. Crystallogr</journal-id><journal-id journal-id-type="doi">10.1107/S13990047</journal-id><journal-id journal-id-type="publisher-id">AYD2</journal-id><journal-title-group><journal-title>Acta Crystallographica Section D: Biological Crystallography</journal-title></journal-title-group><issn pub-type="ppub">0907-4449</issn><issn pub-type="epub">1399-0047</issn><publisher><publisher-name>Munksgaard International Publishers</publisher-name><publisher-loc>5 Abbey Square, Chester, Cheshire CH1 2HU, England</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12925794</article-id><article-id pub-id-type="pmc">7161644</article-id><article-id pub-id-type="doi">10.1107/S0907444903016779</article-id><article-id pub-id-type="publisher-id">AYDBE0025</article-id><article-id pub-id-type="other">be0025</article-id><article-categories><subj-group subj-group-type="heading"><subject>Structural Genomics Papers</subject></subj-group></article-categories><title-group><article-title>Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein</article-title><alt-title alt-title-type="left-running-head">Campanacci <italic>et al.</italic></alt-title><alt-title alt-title-type="right-running-head">SARS coronavirus Nsp9 protein</alt-title></title-group><contrib-group><contrib id="cr1" contrib-type="author"><name><surname>Campanacci</surname><given-names>Valérie</given-names></name></contrib><contrib id="cr2" contrib-type="author"><name><surname>Egloff</surname><given-names>Marie‐Pierre</given-names></name></contrib><contrib id="cr3" contrib-type="author"><name><surname>Longhi</surname><given-names>Sonia</given-names></name></contrib><contrib id="cr4" contrib-type="author"><name><surname>Ferron</surname><given-names>François</given-names></name></contrib><contrib id="cr5" contrib-type="author"><name><surname>Rancurel</surname><given-names>Corinne</given-names></name></contrib><contrib id="cr6" contrib-type="author"><name><surname>Salomoni</surname><given-names>Aurelia</given-names></name></contrib><contrib id="cr7" contrib-type="author"><name><surname>Durousseau</surname><given-names>Cécile</given-names></name></contrib><contrib id="cr8" contrib-type="author"><name><surname>Tocque</surname><given-names>Fabienne</given-names></name></contrib><contrib id="cr9" contrib-type="author"><name><surname>Brémond</surname><given-names>Nicolas</given-names></name></contrib><contrib id="cr10" contrib-type="author"><name><surname>Dobbe</surname><given-names>Jessika C.</given-names></name></contrib><contrib id="cr11" contrib-type="author"><name><surname>Snijder</surname><given-names>Eric J.</given-names></name></contrib><contrib id="cr12" contrib-type="author"><name><surname>Canard</surname><given-names>Bruno</given-names></name></contrib><contrib id="cr13" contrib-type="author"><name><surname>Cambillau</surname><given-names>Christian</given-names></name></contrib></contrib-group><aff id="a1"><label><sup>1</sup></label>Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and Universités Aix‐Marseille I and II, 31 Chemin Joseph Aiguier, 13402 Marseille CEDEX 20, France</aff><aff id="a2"><label><sup>2</sup></label>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands</aff><author-notes><corresp id="correspondenceTo">Bruno Canard, e‐mail: <email>canard@afmb.cnrs-mrs.fr</email>; Christian Cambillau, e‐mail: <email>cambillau@afmb.cnrs-mrs.fr</email></corresp></author-notes><pub-date pub-type="epub"><day>07</day><month>6</month><year>2004</year></pub-date><pub-date pub-type="ppub"><month>9</month><year>2003</year></pub-date><volume>59</volume><issue>9</issue><issue-id pub-id-type="doi">10.1111/ayd.2003.59.issue-9</issue-id><fpage>1628</fpage><lpage>1631</lpage><history><pmc-comment>supplied string: Received 12 July 2003, accepted 30 July 2003</pmc-comment>
<date date-type="received"><day>12</day><month>7</month><year>2003</year></date><date date-type="accepted"><day>30</day><month>7</month><year>2003</year></date></history><permissions><license><license-p>This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.</license-p></license></permissions><abstract><p>The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive‐stranded RNA virus (SARS‐CoV) belonging to the <italic>Coronaviridae</italic> family and its genome differs substantially from those of other known coronaviruses. SARS‐CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS‐CoV whole‐genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20–30 mature proteins. The functions of many of these polypeptides, including the Nsp9–Nsp10 replicase‐cleavage products, are still unknown. Here, the cloning, <italic>Escherichia coli</italic> expression, purification and crystallization of the SARS‐CoV Nsp9 protein, the first SARS‐CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 Å resolution and belong to space group <italic>P</italic>6<sub>1/5</sub>22, with unit‐cell parameters <italic>a</italic> = <italic>b</italic> = 89.7, <italic>c</italic> = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (<italic>V</italic><sub>M</sub> = 3.1 Å<sup>3</sup> Da<sup>−1</sup>).</p></abstract><kwd-group><kwd id="k1">SARS coronavirus</kwd><kwd id="k2">Nsp9.</kwd></kwd-group><counts><count count-type="links-crossref" count="0"></count><count count-type="links-pubmed" count="0"></count><fig-count count="0"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="0"></ref-count><word-count count="0"></word-count></counts><custom-meta-group><custom-meta><meta-name>source-schema-version-number</meta-name><meta-value>2.0</meta-value></custom-meta><custom-meta><meta-name>cover-date</meta-name><meta-value>September 2003</meta-value></custom-meta><custom-meta><meta-name>details-of-publishers-convertor</meta-name><meta-value>Converter:WILEY_ML3GV2_TO_JATSPMC version:5.8.0 mode:remove_FC converted:15.04.2020</meta-value></custom-meta></custom-meta-group></article-meta></front><body><p>The full text for this article, hosted at <ext-link ext-link-type="uri" xlink:href="http://journals.iucr.org">http://journals.iucr.org</ext-link>, is unavailable due to technical difficulties.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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