Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy.
Identifieur interne : 000995 ( Pmc/Corpus ); précédent : 000994; suivant : 000996Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy.
Auteurs : Marta Colaneri ; Elena Seminari ; Antonio Piralla ; Valentina Zuccaro ; Alessandro Di Filippo ; Fausto Baldanti ; Raffaele Bruno ; Mario U. MondelliSource :
- The Journal of Hospital Infection [ 0195-6701 ] ; 2020.
Url:
DOI: 10.1016/j.jhin.2020.03.018
PubMed: 32201338
PubMed Central: 7156210
Links to Exploration step
PMC:7156210Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy.</title><author><name sortKey="Colaneri, Marta" sort="Colaneri, Marta" uniqKey="Colaneri M" first="Marta" last="Colaneri">Marta Colaneri</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Seminari, Elena" sort="Seminari, Elena" uniqKey="Seminari E" first="Elena" last="Seminari">Elena Seminari</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Piralla, Antonio" sort="Piralla, Antonio" uniqKey="Piralla A" first="Antonio" last="Piralla">Antonio Piralla</name><affiliation><nlm:aff id="aff2">Molecular Virology Unit, Microbiology and Virology</nlm:aff></affiliation></author><author><name sortKey="Zuccaro, Valentina" sort="Zuccaro, Valentina" uniqKey="Zuccaro V" first="Valentina" last="Zuccaro">Valentina Zuccaro</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Filippo, Alessandro Di" sort="Filippo, Alessandro Di" uniqKey="Filippo A" first="Alessandro Di" last="Filippo">Alessandro Di Filippo</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Baldanti, Fausto" sort="Baldanti, Fausto" uniqKey="Baldanti F" first="Fausto" last="Baldanti">Fausto Baldanti</name><affiliation><nlm:aff id="aff2">Molecular Virology Unit, Microbiology and Virology</nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><italic>Department of Clinical</italic><italic>,</italic> Surgical<italic>,</italic> Diagnostic, and Paediatric Sciences<italic>,</italic> University of Pavia</nlm:aff></affiliation></author><author><name sortKey="Bruno, Raffaele" sort="Bruno, Raffaele" uniqKey="Bruno R" first="Raffaele" last="Bruno">Raffaele Bruno</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><italic>Department of Clinical</italic><italic>,</italic> Surgical<italic>,</italic> Diagnostic, and Paediatric Sciences<italic>,</italic> University of Pavia</nlm:aff></affiliation></author><author><name sortKey="Mondelli, Mario U" sort="Mondelli, Mario U" uniqKey="Mondelli M" first="Mario U." last="Mondelli">Mario U. Mondelli</name><affiliation><nlm:aff id="aff4">Division of Infectious Diseases II and Immunology, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32201338</idno><idno type="pmc">7156210</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156210</idno><idno type="RBID">PMC:7156210</idno><idno type="doi">10.1016/j.jhin.2020.03.018</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000995</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000995</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy.</title><author><name sortKey="Colaneri, Marta" sort="Colaneri, Marta" uniqKey="Colaneri M" first="Marta" last="Colaneri">Marta Colaneri</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Seminari, Elena" sort="Seminari, Elena" uniqKey="Seminari E" first="Elena" last="Seminari">Elena Seminari</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Piralla, Antonio" sort="Piralla, Antonio" uniqKey="Piralla A" first="Antonio" last="Piralla">Antonio Piralla</name><affiliation><nlm:aff id="aff2">Molecular Virology Unit, Microbiology and Virology</nlm:aff></affiliation></author><author><name sortKey="Zuccaro, Valentina" sort="Zuccaro, Valentina" uniqKey="Zuccaro V" first="Valentina" last="Zuccaro">Valentina Zuccaro</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Filippo, Alessandro Di" sort="Filippo, Alessandro Di" uniqKey="Filippo A" first="Alessandro Di" last="Filippo">Alessandro Di Filippo</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation></author><author><name sortKey="Baldanti, Fausto" sort="Baldanti, Fausto" uniqKey="Baldanti F" first="Fausto" last="Baldanti">Fausto Baldanti</name><affiliation><nlm:aff id="aff2">Molecular Virology Unit, Microbiology and Virology</nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><italic>Department of Clinical</italic><italic>,</italic> Surgical<italic>,</italic> Diagnostic, and Paediatric Sciences<italic>,</italic> University of Pavia</nlm:aff></affiliation></author><author><name sortKey="Bruno, Raffaele" sort="Bruno, Raffaele" uniqKey="Bruno R" first="Raffaele" last="Bruno">Raffaele Bruno</name><affiliation><nlm:aff id="aff1">Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><italic>Department of Clinical</italic><italic>,</italic> Surgical<italic>,</italic> Diagnostic, and Paediatric Sciences<italic>,</italic> University of Pavia</nlm:aff></affiliation></author><author><name sortKey="Mondelli, Mario U" sort="Mondelli, Mario U" uniqKey="Mondelli M" first="Mario U." last="Mondelli">Mario U. Mondelli</name><affiliation><nlm:aff id="aff4">Division of Infectious Diseases II and Immunology, Fondazione IRCCS Policlinico San Matteo</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy</nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Hospital Infection</title><idno type="ISSN">0195-6701</idno><idno type="eISSN">1532-2939</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rabenau, H F" uniqKey="Rabenau H">H.F. Rabenau</name></author><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J. Cinatl</name></author><author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B. Morgenstern</name></author><author><name sortKey="Bauer, G" uniqKey="Bauer G">G. Bauer</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W. Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bin, S Y" uniqKey="Bin S">S.Y. Bin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A" uniqKey="Lai A">A. Lai</name></author><author><name sortKey="Bergna, A" uniqKey="Bergna A">A. Bergna</name></author><author><name sortKey="Acciarri, C" uniqKey="Acciarri C">C. Acciarri</name></author><author><name sortKey="Galli, M" uniqKey="Galli M">M. Galli</name></author><author><name sortKey="Zehender, G" uniqKey="Zehender G">G. Zehender</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Hosp Infect</journal-id><journal-id journal-id-type="iso-abbrev">J. Hosp. Infect</journal-id><journal-title-group><journal-title>The Journal of Hospital Infection</journal-title></journal-title-group><issn pub-type="ppub">0195-6701</issn><issn pub-type="epub">1532-2939</issn><publisher><publisher-name>The Healthcare Infection Society. Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32201338</article-id><article-id pub-id-type="pmc">7156210</article-id><article-id pub-id-type="publisher-id">S0195-6701(20)30117-1</article-id><article-id pub-id-type="doi">10.1016/j.jhin.2020.03.018</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Lack of SARS-CoV-2 RNA environmental contamination in a tertiary referral hospital for infectious diseases in Northern Italy.</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Colaneri</surname><given-names>Marta</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="cor1" ref-type="corresp">∗</xref><xref rid="fn1" ref-type="fn">∗</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Seminari</surname><given-names>Elena</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="fn1" ref-type="fn">∗</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Piralla</surname><given-names>Antonio</given-names></name><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Zuccaro</surname><given-names>Valentina</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>Filippo</surname><given-names>Alessandro Di</given-names></name><xref rid="aff1" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="au6"><name><surname>Baldanti</surname><given-names>Fausto</given-names></name><xref rid="aff2" ref-type="aff">2</xref><xref rid="aff3" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="au7"><name><surname>Bruno</surname><given-names>Raffaele</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff3" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="au8"><name><surname>Mondelli</surname><given-names>Mario U.</given-names></name><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff5" ref-type="aff">5</xref></contrib><contrib contrib-type="author"><collab>COVID19 IRCCS San Matteo Pavia Task Force<contrib-group><contrib contrib-type="author" id="au9"><name><surname>Bruno</surname><given-names>Raffaele</given-names></name></contrib><contrib contrib-type="author" id="au10"><name><surname>Mondelli</surname><given-names>Mario U.</given-names></name></contrib><contrib contrib-type="author" id="au11"><name><surname>Brunetti</surname><given-names>Enrico</given-names></name></contrib><contrib contrib-type="author" id="au12"><name><surname>Di Matteo</surname><given-names>Angela</given-names></name></contrib><contrib contrib-type="author" id="au13"><name><surname>Seminari</surname><given-names>Elena</given-names></name></contrib><contrib contrib-type="author" id="au14"><name><surname>Maiocchi</surname><given-names>Laura</given-names></name></contrib><contrib contrib-type="author" id="au15"><name><surname>Zuccaro</surname><given-names>Valentina</given-names></name></contrib><contrib contrib-type="author" id="au16"><name><surname>Pagnucco</surname><given-names>Layla</given-names></name></contrib><contrib contrib-type="author" id="au17"><name><surname>Mariani</surname><given-names>Bianca</given-names></name></contrib><contrib contrib-type="author" id="au18"><name><surname>Ludovisi</surname><given-names>Serena</given-names></name></contrib><contrib contrib-type="author" id="au19"><name><surname>Lissandrin</surname><given-names>Raffaella</given-names></name></contrib><contrib contrib-type="author" id="au20"><name><surname>Parisi</surname><given-names>Aldo</given-names></name></contrib><contrib contrib-type="author" id="au21"><name><surname>Sacchi</surname><given-names>Paolo</given-names></name></contrib><contrib contrib-type="author" id="au22"><name><surname>Patruno</surname><given-names>Savino FA.</given-names></name></contrib><contrib contrib-type="author" id="au23"><name><surname>Michelone</surname><given-names>Giuseppe</given-names></name></contrib><contrib contrib-type="author" id="au24"><name><surname>Gulminetti</surname><given-names>Roberto</given-names></name></contrib><contrib contrib-type="author" id="au25"><name><surname>Zanaboni</surname><given-names>Domenico</given-names></name></contrib><contrib contrib-type="author" id="au26"><name><surname>Novati</surname><given-names>Stefano</given-names></name></contrib><contrib contrib-type="author" id="au27"><name><surname>Maserati</surname><given-names>Renato</given-names></name></contrib><contrib contrib-type="author" id="au28"><name><surname>Orsolini</surname><given-names>Paolo</given-names></name></contrib><contrib contrib-type="author" 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id="au139"><name><surname>Marena</surname><given-names>Carlo</given-names></name></contrib><contrib contrib-type="author" id="au140"><name><surname>Muzzi</surname><given-names>Alba</given-names></name></contrib><contrib contrib-type="author" id="au141"><name><surname>Lago</surname><given-names>Paolo</given-names></name></contrib><contrib contrib-type="author" id="au142"><name><surname>Comandatore</surname><given-names>Francesco</given-names></name></contrib><contrib contrib-type="author" id="au143"><name><surname>Bissignandi</surname><given-names>Gherard</given-names></name></contrib><contrib contrib-type="author" id="au144"><name><surname>Gaiarsa</surname><given-names>Stefano</given-names></name></contrib><contrib contrib-type="author" id="au145"><name><surname>Rettani</surname><given-names>Marco</given-names></name></contrib><contrib contrib-type="author" id="au146"><name><surname>Bandi</surname><given-names>Claudio</given-names></name></contrib></contrib-group></collab></contrib></contrib-group><aff id="aff1"><label>1</label>Division of Infectious Diseases I, Fondazione IRCCS Policlinico San Matteo</aff><aff id="aff2"><label>2</label>Molecular Virology Unit, Microbiology and Virology</aff><aff id="aff3"><label>3</label><italic>Department of Clinical</italic><italic>,</italic> Surgical<italic>,</italic> Diagnostic, and Paediatric Sciences<italic>,</italic> University of Pavia</aff><aff id="aff4"><label>4</label>Division of Infectious Diseases II and Immunology, Fondazione IRCCS Policlinico San Matteo</aff><aff id="aff5"><label>5</label>Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy</aff><author-notes><corresp id="cor1"><label>∗</label><bold>Corresponding author</bold>: Malattie Infettive II, Infettivologia ed Immunologia, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 2, 27100 Pavia, Italy.</corresp><fn id="fn1"><label>∗</label><p id="ntpara0010">These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="pmc-release"><day>19</day><month>3</month><year>2020</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="epub"><day>19</day><month>3</month><year>2020</year></pub-date><history><date date-type="received"><day>11</day><month>3</month><year>2020</year></date><date date-type="accepted"><day>13</day><month>3</month><year>2020</year></date></history><permissions><copyright-statement>© 2020 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2020</copyright-year><copyright-holder>The Healthcare Infection Society</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="p0010">The World Health Organization defined Coronavirus Disease 2019 (COVID-19) as the disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a betacoronavirus belonging to the same subgenus as the severe acute respiratory syndrome Coronavirus (SARS-CoV). Human coronaviruses (HCoVs) spread in a similar fashion as Rhinoviruses, by direct contact with infected secretions or large aerosol droplets [<xref rid="bib1" ref-type="bibr">1</xref>]. Health care workers are at increased risk of acquiring COVID-19 infection, possibly due to direct contact with the patients. Indeed, transmission of HCoVs through environmental contamination has been reported in healthcare settings [<xref rid="bib2" ref-type="bibr">2</xref>]. Understanding which are the potentially contaminated surfaces in a healthcare environment is crucial to protect healthcare workers from this virus showing an unprecedented exponential trend with a doubling time of 3.6-4.1 days [<xref rid="bib3" ref-type="bibr">3</xref>]. In this regard, studies suggest that surfaces and suspensions can carry HCoVs, increasing the risk of contact transmission that could lead to hospital acquired HCoVs infections [<xref rid="bib4" ref-type="bibr">4</xref>,<xref rid="bib5" ref-type="bibr">5</xref>]. Otter et al. found that other coronaviruses (SARS-CoV, MERS-CoV) can be found on plastic, metal and cloths for up to 6 days [<xref rid="bib6" ref-type="bibr">6</xref>]. Thus, monitoring environmental contamination of SARS-CoV-2 can support investigation of the current outbreak and benefit the management of COVID-19 infection. In addition, it may help on assessing the effectiveness of disinfection procedures and safety of personal protective equipment (PPE).</p><p id="p0015">Since February 21, 2020, when the first autochthonous case in Italy was confirmed, an overwhelming number of SARS-CoV-2 infections is continuously being detected, exceeding 8,000 cases at the time of writing. Fondazione IRCCS Policlinico San Matteo, Pavia, is a 1,300-bed tertiary teaching hospital in Northern Italy and a national SARS-CoV-2 referral center. The hospital houses 23 ICU beds and 44 Infectious Diseases (ID) beds, the latter being distributed in two floors. In the ID ward, each room has a buffer zone to allow safe donning and disposal of PPE. Healthcare workers involved in the direct care of patients use the following PPE: liquid-repelling gowns, double gloves, a class 2 filtering face-piece respirator (FFP2) and eye protection (goggles or face shield). Cleaning procedures have been standardized [<xref rid="bib7" ref-type="bibr">7</xref>], in particular ward surfaces are cleaned with sodium hypochloride at the concentration of 1,000 ppM of free chlorine (0.1%) daily and 5,000 ppM of free chlorine (0.5%) in terminal sanitization.</p><p id="p0020">From February 21 to 29, 580 cases of SARS-CoV-2 were identified by the Virology laboratory, and those with interstitial pneumonia were admitted. Samples were collected on 28<sup>th</sup> February; by that day 100% of admitted patients were COVID-19 positive with pneumonia, and were treated with C-PAP or high flux oxygen.</p><p id="p0025">Surfaces in areas considered virus free were swabbed to search for COVID-19 RNA. <xref rid="tbl1" ref-type="table">Table 1</xref>indicates which surfaces and objects were subjected to swabbing and <xref rid="fig1" ref-type="fig">Fig. 1</xref>illustrates the map of the ward where the test was carried out. Environmental samples were obtained by using a sterile flexible nasopharyngeal nylon flocked premoistened swabs (FLOQSwabs™, Copan Italia, Brescia, Italy) dipped in 3 ml universal transport medium (UTM™, Copan Italia, Brescia, Italy). Total nucleic acids (DNA/RNA) were extracted from 200 μl of UTM™ using the QIAsymphony® instrument with QIAsymphony® DSP Virus/Pathogen Midi Kit (Complex 400 protocol) according to the manufacturer’s instructions (QIAGEN, Qiagen, Hilden, Germany). Specific real-time reverse transcriptase–polymerase chain reaction (RT-PCR) targeting RNA-dependent RNA polymerase and E genes were used to detect the presence of SARS-CoV-2 according to WHO guidelines [<xref rid="bib8" ref-type="bibr">8</xref>] and Corman et al. protocols [<xref rid="bib9" ref-type="bibr">9</xref>].<table-wrap position="float" id="tbl1"><label>Table 1</label><caption><p>List of objects and surfaces swabbed for SARS-CoV-2 RNA.</p></caption><alt-text id="alttext0015">Table 1</alt-text><table frame="hsides" rules="groups"><thead><tr><th>High risk of contamination area</th><th>Inanimate surfaces</th></tr></thead><tbody><tr><td rowspan="4"><bold>Buffer zone od patients room</bold></td><td>Door handles</td></tr><tr><td>Waste container covers</td></tr><tr><td>Sink handles</td></tr><tr><td>Wall surfaces</td></tr><tr><td rowspan="6"><bold>Doctors’ and nurses’ lounge</bold></td><td>Kitchen table and sink</td></tr><tr><td>Desks</td></tr><tr><td>Computer keyboads</td></tr><tr><td>Medical charts and parameters tabs</td></tr><tr><td>Door handles</td></tr><tr><td>Therapy trolleys</td></tr><tr><td><bold>Staff personal belongings</bold></td><td>Mobile phones</td></tr></tbody></table></table-wrap><fig id="fig1"><label>Figure 1</label><caption><p>Map of the ward where swabs for SARS-CoV-2 RNA were obtained. Red dots indicate the sites where swabs were applied.</p></caption><alt-text id="alttext0010">Figure 1</alt-text><graphic xlink:href="gr1_lrg"></graphic></fig></p><p id="p0030">Sixteen swabs were collected from inanimate surfaces at high risk of contamination inside the wards. All inanimate surfaces and materials at high risk of contamination were free of SARS-CoV-2 RNA. At the time of writing no cases of COVID-19 has been detected in the staff involved in the patient care. While this of course does not exclude the risk of transmission, it does provide evidence that the protective measures implemented in our setting significantly decrease the risk of environmental contamination and reduce concerns over healthcare workers contamination and infection, at least from inanimate surfaces in areas that are either preserved as clean or decontaminated. Our findings therefore validate our cleaning and disinfection policies and confirm an adequate use of PPE.</p><p id="p0035">These data are in keeping with very recent findings reporting that anterooms, corridors and post-cleaning samples were negative for SARS-CoV-2 RNA, suggesting that current protections and decontamination procedures are sufficient despite extensive contamination of inanimate surfaces in patients’ room and toilet sites [<xref rid="bib8" ref-type="bibr">8</xref>]. In any case, environmental contamination with SARS-CoV-2 through respiratory droplets and fecal shedding suggests that the environment is indeed a potential medium of transmission [<xref rid="bib10" ref-type="bibr">10</xref>]. Our study has limitations since virus viability was not shown and the sample size was small, due to difficulty to run more tests in a time of emergency. A more precise evaluation of the infectious potential of the environment could be undertaken by collecting droplets from patients’ sputum on inanimate surfaces and measuring SARS-CoV-2 infectious potential <italic>in vitro</italic> as a function of time. However, the emergency of the outbreak required a fast assessment of the current situation. 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