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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural Genomics of the Severe Acute Respiratory Syndrome Coronavirus: Nuclear Magnetic Resonance Structure of the Protein nsP7</title>
<author><name sortKey="Peti, Wolfgang" sort="Peti, Wolfgang" uniqKey="Peti W" first="Wolfgang" last="Peti">Wolfgang Peti</name>
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<author><name sortKey="Johnson, Margaret A" sort="Johnson, Margaret A" uniqKey="Johnson M" first="Margaret A." last="Johnson">Margaret A. Johnson</name>
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<author><name sortKey="Herrmann, Torsten" sort="Herrmann, Torsten" uniqKey="Herrmann T" first="Torsten" last="Herrmann">Torsten Herrmann</name>
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<author><name sortKey="Neuman, Benjamin W" sort="Neuman, Benjamin W" uniqKey="Neuman B" first="Benjamin W." last="Neuman">Benjamin W. Neuman</name>
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<author><name sortKey="Buchmeier, Michael J" sort="Buchmeier, Michael J" uniqKey="Buchmeier M" first="Michael J." last="Buchmeier">Michael J. Buchmeier</name>
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<author><name sortKey="Nelson, Mike" sort="Nelson, Mike" uniqKey="Nelson M" first="Mike" last="Nelson">Mike Nelson</name>
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<author><name sortKey="Joseph, Jeremiah" sort="Joseph, Jeremiah" uniqKey="Joseph J" first="Jeremiah" last="Joseph">Jeremiah Joseph</name>
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<author><name sortKey="Page, Rebecca" sort="Page, Rebecca" uniqKey="Page R" first="Rebecca" last="Page">Rebecca Page</name>
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<author><name sortKey="Stevens, Raymond C" sort="Stevens, Raymond C" uniqKey="Stevens R" first="Raymond C." last="Stevens">Raymond C. Stevens</name>
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<author><name sortKey="Kuhn, Peter" sort="Kuhn, Peter" uniqKey="Kuhn P" first="Peter" last="Kuhn">Peter Kuhn</name>
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<author><name sortKey="Wuthrich, Kurt" sort="Wuthrich, Kurt" uniqKey="Wuthrich K" first="Kurt" last="Wüthrich">Kurt Wüthrich</name>
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<idno type="pmc">1235862</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1235862</idno>
<idno type="RBID">PMC:1235862</idno>
<idno type="doi">10.1128/JVI.79.20.12905-12913.2005</idno>
<date when="2005">2005</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural Genomics of the Severe Acute Respiratory Syndrome Coronavirus: Nuclear Magnetic Resonance Structure of the Protein nsP7</title>
<author><name sortKey="Peti, Wolfgang" sort="Peti, Wolfgang" uniqKey="Peti W" first="Wolfgang" last="Peti">Wolfgang Peti</name>
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<affiliation><nlm:aff id="aff1"></nlm:aff>
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<author><name sortKey="Johnson, Margaret A" sort="Johnson, Margaret A" uniqKey="Johnson M" first="Margaret A." last="Johnson">Margaret A. Johnson</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Herrmann, Torsten" sort="Herrmann, Torsten" uniqKey="Herrmann T" first="Torsten" last="Herrmann">Torsten Herrmann</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Neuman, Benjamin W" sort="Neuman, Benjamin W" uniqKey="Neuman B" first="Benjamin W." last="Neuman">Benjamin W. Neuman</name>
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</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Buchmeier, Michael J" sort="Buchmeier, Michael J" uniqKey="Buchmeier M" first="Michael J." last="Buchmeier">Michael J. Buchmeier</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nelson, Mike" sort="Nelson, Mike" uniqKey="Nelson M" first="Mike" last="Nelson">Mike Nelson</name>
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</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Joseph, Jeremiah" sort="Joseph, Jeremiah" uniqKey="Joseph J" first="Jeremiah" last="Joseph">Jeremiah Joseph</name>
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<affiliation><nlm:aff id="aff1"></nlm:aff>
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</author>
<author><name sortKey="Page, Rebecca" sort="Page, Rebecca" uniqKey="Page R" first="Rebecca" last="Page">Rebecca Page</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Stevens, Raymond C" sort="Stevens, Raymond C" uniqKey="Stevens R" first="Raymond C." last="Stevens">Raymond C. Stevens</name>
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</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kuhn, Peter" sort="Kuhn, Peter" uniqKey="Kuhn P" first="Peter" last="Kuhn">Peter Kuhn</name>
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</affiliation>
<affiliation><nlm:aff id="aff1"></nlm:aff>
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<affiliation><nlm:aff id="aff1"></nlm:aff>
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</author>
<author><name sortKey="Wuthrich, Kurt" sort="Wuthrich, Kurt" uniqKey="Wuthrich K" first="Kurt" last="Wüthrich">Kurt Wüthrich</name>
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<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2005">2005</date>
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<front><div type="abstract" xml:lang="en"><p>Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily of helical secondary structures. Three helices (α2 to α4) form a flat up-down-up antiparallel α-helix sheet. The N-terminal segment of residues 1 to 22, containing two turns of α-helix and one turn of 3<sub>10</sub>
-helix, is packed across the surface of α2 and α3 in the helix sheet, with the α-helical region oriented at a 60° angle relative to α2 and α3. The surface charge distribution is pronouncedly asymmetrical, with the flat surface of the helical sheet showing a large negatively charged region adjacent to a large hydrophobic patch and the opposite side containing a positively charged groove that extends along the helix α1. Each of these three areas is thus implicated as a potential site for protein-protein interactions.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="publisher-id">jvi</journal-id>
<journal-title>Journal of Virology</journal-title>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">16188992</article-id>
<article-id pub-id-type="pmc">1235862</article-id>
<article-id pub-id-type="publisher-id">1191-05</article-id>
<article-id pub-id-type="doi">10.1128/JVI.79.20.12905-12913.2005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Structure and Assembly</subject>
</subj-group>
</article-categories>
<title-group><article-title>Structural Genomics of the Severe Acute Respiratory Syndrome Coronavirus: Nuclear Magnetic Resonance Structure of the Protein nsP7</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Peti</surname>
<given-names>Wolfgang</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="fn" rid="fn1">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Johnson</surname>
<given-names>Margaret A.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Herrmann</surname>
<given-names>Torsten</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="fn" rid="fn2">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Neuman</surname>
<given-names>Benjamin W.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Buchmeier</surname>
<given-names>Michael J.</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nelson</surname>
<given-names>Mike</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Joseph</surname>
<given-names>Jeremiah</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Page</surname>
<given-names>Rebecca</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">5</xref>
<xref ref-type="fn" rid="fn3">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Stevens</surname>
<given-names>Raymond C.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kuhn</surname>
<given-names>Peter</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">4</xref>
<xref ref-type="aff" rid="aff1">5</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wüthrich</surname>
<given-names>Kurt</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff1">2</xref>
<xref ref-type="aff" rid="aff1">5</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="aff1">Department of Molecular Biology,<label>1</label>
Consortium for Functional and Structural Proteomics of SARS-CoV Related Proteins,<label>2</label>
Department of Neuropharmacology,<label>3</label>
Department of Cell Biology,<label>4</label>
Joint Center for Structural Genomics, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, California 92037<label>5</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address for Peter Kuhn: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., CB-265, La Jolla, CA 92037. Phone: (858) 784-9114. Fax: (858) 784-8996. E-mail: <email>pkuhn@scripps.edu</email>
. Mailing address for Kurt Wüthrich: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., MB-44, La Jolla, CA 92037. Phone: (858) 784-8011. Fax: (858) 784-8014. E-mail: <email>wuthrich@scripps.edu</email>
.</p>
</fn>
<fn id="fn1"><label>†</label>
<p>Present address: Brown University, Department of Molecular Pharmacology, Physiology and Biotechnology, 70 Ship Street, GE-3, Providence, RI 02912.</p>
</fn>
<fn id="fn2"><label>‡</label>
<p>Present address: Institut für Molekularbiologie und Biophysik, ETH Zürich, CH-8093 Zürich, Switzerland.</p>
</fn>
<fn id="fn3"><label>§</label>
<p>Present address: Brown University, Department of Molecular Biology, Cell Biology and Biochemistry, 70 Ship Street, GE-4, Providence, RI 02912.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>10</month>
<year>2005</year>
</pub-date>
<volume>79</volume>
<issue>20</issue>
<fpage>12905</fpage>
<lpage>12913</lpage>
<history><date date-type="received"><day>9</day>
<month>6</month>
<year>2005</year>
</date>
<date date-type="accepted"><day>22</day>
<month>7</month>
<year>2005</year>
</date>
</history>
<copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement>
<copyright-year>2005</copyright-year>
<abstract><p>Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily of helical secondary structures. Three helices (α2 to α4) form a flat up-down-up antiparallel α-helix sheet. The N-terminal segment of residues 1 to 22, containing two turns of α-helix and one turn of 3<sub>10</sub>
-helix, is packed across the surface of α2 and α3 in the helix sheet, with the α-helical region oriented at a 60° angle relative to α2 and α3. The surface charge distribution is pronouncedly asymmetrical, with the flat surface of the helical sheet showing a large negatively charged region adjacent to a large hydrophobic patch and the opposite side containing a positively charged groove that extends along the helix α1. Each of these three areas is thus implicated as a potential site for protein-protein interactions.</p>
</abstract>
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</front>
</pmc>
</record>
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