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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An Exposed Domain in the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Induces Neutralizing Antibodies</title><author><name sortKey="Zhou, Tong" sort="Zhou, Tong" uniqKey="Zhou T" first="Tong" last="Zhou">Tong Zhou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hong" sort="Wang, Hong" uniqKey="Wang H" first="Hong" last="Wang">Hong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Luo, Danlin" sort="Luo, Danlin" uniqKey="Luo D" first="Danlin" last="Luo">Danlin Luo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rowe, Thomas" sort="Rowe, Thomas" uniqKey="Rowe T" first="Thomas" last="Rowe">Thomas Rowe</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Zheng" sort="Wang, Zheng" uniqKey="Wang Z" first="Zheng" last="Wang">Zheng Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hogan, Robert J" sort="Hogan, Robert J" uniqKey="Hogan R" first="Robert J." last="Hogan">Robert J. Hogan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Qiu, Shihong" sort="Qiu, Shihong" uniqKey="Qiu S" first="Shihong" last="Qiu">Shihong Qiu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bunzel, Robert J" sort="Bunzel, Robert J" uniqKey="Bunzel R" first="Robert J." last="Bunzel">Robert J. Bunzel</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, Guoqiang" sort="Huang, Guoqiang" uniqKey="Huang G" first="Guoqiang" last="Huang">Guoqiang Huang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mishra, Vinod" sort="Mishra, Vinod" uniqKey="Mishra V" first="Vinod" last="Mishra">Vinod Mishra</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Voss, Thomas G" sort="Voss, Thomas G" uniqKey="Voss T" first="Thomas G." last="Voss">Thomas G. Voss</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kimberly, Robert" sort="Kimberly, Robert" uniqKey="Kimberly R" first="Robert" last="Kimberly">Robert Kimberly</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15194798</idno><idno type="pmc">421657</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC421657</idno><idno type="RBID">PMC:421657</idno><idno type="doi">10.1128/JVI.78.13.7217-7226.2004</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000682</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000682</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An Exposed Domain in the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Induces Neutralizing Antibodies</title><author><name sortKey="Zhou, Tong" sort="Zhou, Tong" uniqKey="Zhou T" first="Tong" last="Zhou">Tong Zhou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hong" sort="Wang, Hong" uniqKey="Wang H" first="Hong" last="Wang">Hong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Luo, Danlin" sort="Luo, Danlin" uniqKey="Luo D" first="Danlin" last="Luo">Danlin Luo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rowe, Thomas" sort="Rowe, Thomas" uniqKey="Rowe T" first="Thomas" last="Rowe">Thomas Rowe</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Zheng" sort="Wang, Zheng" uniqKey="Wang Z" first="Zheng" last="Wang">Zheng Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hogan, Robert J" sort="Hogan, Robert J" uniqKey="Hogan R" first="Robert J." last="Hogan">Robert J. Hogan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Qiu, Shihong" sort="Qiu, Shihong" uniqKey="Qiu S" first="Shihong" last="Qiu">Shihong Qiu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bunzel, Robert J" sort="Bunzel, Robert J" uniqKey="Bunzel R" first="Robert J." last="Bunzel">Robert J. Bunzel</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, Guoqiang" sort="Huang, Guoqiang" uniqKey="Huang G" first="Guoqiang" last="Huang">Guoqiang Huang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mishra, Vinod" sort="Mishra, Vinod" uniqKey="Mishra V" first="Vinod" last="Mishra">Vinod Mishra</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Voss, Thomas G" sort="Voss, Thomas G" uniqKey="Voss T" first="Thomas G." last="Voss">Thomas G. Voss</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kimberly, Robert" sort="Kimberly, Robert" uniqKey="Kimberly R" first="Robert" last="Kimberly">Robert Kimberly</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Exposed epitopes of the spike protein may be recognized by neutralizing antibodies against severe acute respiratory syndrome (SARS) coronavirus (CoV). A protein fragment (S-II) containing predicted epitopes of the spike protein was expressed in <italic>Escherichia coli</italic>. The properly refolded protein fragment specifically bound to the surface of Vero cells. Monoclonal antibodies raised against this fragment recognized the native spike protein of SARS CoV in both monomeric and trimeric forms. These monoclonal antibodies were capable of blocking S-II attachment to Vero cells and exhibited in vitro antiviral activity. These neutralizing antibodies mapped to epitopes in two peptides, each comprising 20 amino acids. Thus, this region of the spike protein might be a target for generation of therapeutic neutralizing antibodies against SARS CoV and for vaccine development to elicit protective humoral immunity.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-id journal-id-type="publisher-id">jvi</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15194798</article-id><article-id pub-id-type="pmc">421657</article-id><article-id pub-id-type="publisher-id">2374-03</article-id><article-id pub-id-type="doi">10.1128/JVI.78.13.7217-7226.2004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Vaccines and Antiviral Agents</subject></subj-group></article-categories><title-group><article-title>An Exposed Domain in the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Induces Neutralizing Antibodies</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Tong</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hong</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Luo</surname><given-names>Danlin</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Rowe</surname><given-names>Thomas</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Zheng</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hogan</surname><given-names>Robert J.</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Qiu</surname><given-names>Shihong</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Bunzel</surname><given-names>Robert J.</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Guoqiang</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Mishra</surname><given-names>Vinod</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Voss</surname><given-names>Thomas G.</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Kimberly</surname><given-names>Robert</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Luo</surname><given-names>Ming</given-names></name><xref ref-type="aff" rid="aff1">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">Division of Clinical Immunology and Rheumatology, Department of Medicine,<label>1</label> Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294,<label>2</label> Division of Homeland Security and Emerging Infectious Diseases Research, Southern Research Institute, Birmingham, Alabama 35205-5305 ,<label>3</label> Southern Biotechnology Associates, Birmingham, Alabama 35209<label>4</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Department of Microbiology, University of Alabama at Birmingham, Room 111, 1025 18th St. South, Birmingham, AL 35294. Phone: (205) 934-4259. Fax: (205) 975-9578. E-mail: <email>mingluo@uab.edu</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2004</year></pub-date><volume>78</volume><issue>13</issue><fpage>7217</fpage><lpage>7226</lpage><history><date date-type="received"><day>12</day><month>11</month><year>2003</year></date><date date-type="accepted"><day>19</day><month>2</month><year>2004</year></date></history><copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement><copyright-year>2004</copyright-year><abstract><p>Exposed epitopes of the spike protein may be recognized by neutralizing antibodies against severe acute respiratory syndrome (SARS) coronavirus (CoV). A protein fragment (S-II) containing predicted epitopes of the spike protein was expressed in <italic>Escherichia coli</italic>. The properly refolded protein fragment specifically bound to the surface of Vero cells. Monoclonal antibodies raised against this fragment recognized the native spike protein of SARS CoV in both monomeric and trimeric forms. These monoclonal antibodies were capable of blocking S-II attachment to Vero cells and exhibited in vitro antiviral activity. These neutralizing antibodies mapped to epitopes in two peptides, each comprising 20 amino acids. Thus, this region of the spike protein might be a target for generation of therapeutic neutralizing antibodies against SARS CoV and for vaccine development to elicit protective humoral immunity.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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