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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Specific epitopes of the structural and hypothetical proteins elicit variable humoral responses in SARS patients</title><author><name sortKey="Chow, S C S" sort="Chow, S C S" uniqKey="Chow S" first="S C S" last="Chow">S C S. Chow</name></author><author><name sortKey="Ho, C Y S" sort="Ho, C Y S" uniqKey="Ho C" first="C Y S" last="Ho">C Y S. Ho</name></author><author><name sortKey="Tam, T T Y" sort="Tam, T T Y" uniqKey="Tam T" first="T T Y" last="Tam">T T Y. Tam</name></author><author><name sortKey="Wu, C" sort="Wu, C" uniqKey="Wu C" first="C" last="Wu">C. Wu</name></author><author><name sortKey="Cheung, T" sort="Cheung, T" uniqKey="Cheung T" first="T" last="Cheung">T. Cheung</name></author><author><name sortKey="Chan, P K S" sort="Chan, P K S" uniqKey="Chan P" first="P K S" last="Chan">P K S. Chan</name></author><author><name sortKey="Ng, M H L" sort="Ng, M H L" uniqKey="Ng M" first="M H L" last="Ng">M H L. Ng</name></author><author><name sortKey="Hui, P K" sort="Hui, P K" uniqKey="Hui P" first="P K" last="Hui">P K Hui</name></author><author><name sortKey="Ng, H K" sort="Ng, H K" uniqKey="Ng H" first="H K" last="Ng">H K Ng</name></author><author><name sortKey="Au, D M Y" sort="Au, D M Y" uniqKey="Au D" first="D M Y" last="Au">D M Y. Au</name></author><author><name sortKey="Lo, A W I" sort="Lo, A W I" uniqKey="Lo A" first="A W I" last="Lo">A W I. Lo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16461566</idno><idno type="pmc">1860290</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1860290</idno><idno type="RBID">PMC:1860290</idno><idno type="doi">10.1136/jcp.2005.029868</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">000659</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000659</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Specific epitopes of the structural and hypothetical proteins elicit variable humoral responses in SARS patients</title><author><name sortKey="Chow, S C S" sort="Chow, S C S" uniqKey="Chow S" first="S C S" last="Chow">S C S. Chow</name></author><author><name sortKey="Ho, C Y S" sort="Ho, C Y S" uniqKey="Ho C" first="C Y S" last="Ho">C Y S. Ho</name></author><author><name sortKey="Tam, T T Y" sort="Tam, T T Y" uniqKey="Tam T" first="T T Y" last="Tam">T T Y. Tam</name></author><author><name sortKey="Wu, C" sort="Wu, C" uniqKey="Wu C" first="C" last="Wu">C. Wu</name></author><author><name sortKey="Cheung, T" sort="Cheung, T" uniqKey="Cheung T" first="T" last="Cheung">T. Cheung</name></author><author><name sortKey="Chan, P K S" sort="Chan, P K S" uniqKey="Chan P" first="P K S" last="Chan">P K S. Chan</name></author><author><name sortKey="Ng, M H L" sort="Ng, M H L" uniqKey="Ng M" first="M H L" last="Ng">M H L. Ng</name></author><author><name sortKey="Hui, P K" sort="Hui, P K" uniqKey="Hui P" first="P K" last="Hui">P K Hui</name></author><author><name sortKey="Ng, H K" sort="Ng, H K" uniqKey="Ng H" first="H K" last="Ng">H K Ng</name></author><author><name sortKey="Au, D M Y" sort="Au, D M Y" uniqKey="Au D" first="D M Y" last="Au">D M Y. Au</name></author><author><name sortKey="Lo, A W I" sort="Lo, A W I" uniqKey="Lo A" first="A W I" last="Lo">A W I. Lo</name></author></analytic><series><title level="j">Journal of Clinical Pathology</title><idno type="ISSN">0021-9746</idno><idno type="eISSN">1472-4146</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Severe acute respiratory syndrome (SARS) is an infectious disease which was caused by a novel coronavirus (SARS‐CoV). SARS has caused an outbreak in the world during 2003 and 2004, with 8098 individuals being infected and a death toll of 774 in 28 regions around the world. Specific humoral responses to viral infection remain unclear.</p></sec><sec><title>Objective</title><p>To analyse the antigenicity of the SARS‐CoV genome and identify potential antigenic epitopes in the structural proteins.</p></sec><sec><title>Methods</title><p>Potential antigenic epitopes were identified in the structural proteins (nucleocapsid, membrane, spike, and small envelope proteins) and hypothetical proteins (SARS3a, 3b, 6, 7a, and 9b) that are specific for SARS‐CoV. A peptide chip platform was created and the profiles of antibodies to these epitopes were investigated in 59 different SARS patients' sera obtained 6–103 days after the onset of the illness. Serial sera from five additional patients were also studied.</p></sec><sec><title>Results</title><p>Epitopes at the N‐terminus of the membrane protein and the C‐terminus of nucleocapsid protein elicited strong antibody responses. Epitopes on the spike protein were only moderately immunogenic but the effects were persistent. Antibodies were also detected for some putative proteins, noticeably the C‐termini of SARS3a and SARS6.</p></sec><sec><title>Conclusions</title><p>Important epitopes of the SARS‐CoV genome that may serve as potential markers for the viral infection are identified. These specific antigenic sites may also be important for vaccine development against this new fatal infectious disease.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Pathol</journal-id><journal-title>Journal of Clinical Pathology</journal-title><issn pub-type="ppub">0021-9746</issn><issn pub-type="epub">1472-4146</issn><publisher><publisher-name>BMJ Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16461566</article-id><article-id pub-id-type="pmc">1860290</article-id><article-id pub-id-type="publisher-id">cp29868</article-id><article-id pub-id-type="doi">10.1136/jcp.2005.029868</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Specific epitopes of the structural and hypothetical proteins elicit variable humoral responses in SARS patients</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chow</surname><given-names>S C S</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ho</surname><given-names>C Y S</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tam</surname><given-names>T T Y</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>C</given-names></name></contrib><contrib contrib-type="author"><name><surname>Cheung</surname><given-names>T</given-names></name></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>P K S</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ng</surname><given-names>M H L</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hui</surname><given-names>P K</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ng</surname><given-names>H K</given-names></name></contrib><contrib contrib-type="author"><name><surname>Au</surname><given-names>D M Y</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lo</surname><given-names>A W I</given-names></name></contrib></contrib-group><aff><bold>S C S Chow</bold>,<bold>T T Y Tam</bold>,<bold>T Cheung</bold>,<bold>D M Y Au</bold>, Century Biotech Ltd, Hong Kong SAR, China</aff><aff><bold>C Y S Ho</bold>,<bold>M H L Ng</bold>,<bold>H K Ng</bold>,<bold>A W I Lo</bold>, Department of Anatomical and Cellular Pathology, Chinese University of Hong Kong</aff><aff><bold>C Wu</bold>, Abgent Inc, San Diego, California, USA</aff><aff><bold>P K S Chan</bold>, Department of Microbiology and School of Public Health, Chinese University of Hong Kong</aff><aff><bold>P K Hui</bold>, Department of Pathology, Kwong Wah Hospital, Hong Kong</aff><author-notes><corresp>Correspondence to: Dr Anthony Lo<break></break>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30‐32 Ngan Shing Street, Shatin, NT, Hong Kong SAR, China; awilo@cuhk.edu.hk</corresp></author-notes><pub-date pub-type="ppub"><month>5</month><year>2006</year></pub-date><volume>59</volume><issue>5</issue><fpage>468</fpage><lpage>476</lpage><history><date date-type="accepted"><day>12</day><month>7</month><year>2005</year></date></history><permissions><copyright-statement>Copyright © 2006 The BMJ Publishing Group and the Association of Clinical Pathologists</copyright-statement></permissions><abstract><sec><title>Background</title><p>Severe acute respiratory syndrome (SARS) is an infectious disease which was caused by a novel coronavirus (SARS‐CoV). SARS has caused an outbreak in the world during 2003 and 2004, with 8098 individuals being infected and a death toll of 774 in 28 regions around the world. Specific humoral responses to viral infection remain unclear.</p></sec><sec><title>Objective</title><p>To analyse the antigenicity of the SARS‐CoV genome and identify potential antigenic epitopes in the structural proteins.</p></sec><sec><title>Methods</title><p>Potential antigenic epitopes were identified in the structural proteins (nucleocapsid, membrane, spike, and small envelope proteins) and hypothetical proteins (SARS3a, 3b, 6, 7a, and 9b) that are specific for SARS‐CoV. A peptide chip platform was created and the profiles of antibodies to these epitopes were investigated in 59 different SARS patients' sera obtained 6–103 days after the onset of the illness. Serial sera from five additional patients were also studied.</p></sec><sec><title>Results</title><p>Epitopes at the N‐terminus of the membrane protein and the C‐terminus of nucleocapsid protein elicited strong antibody responses. Epitopes on the spike protein were only moderately immunogenic but the effects were persistent. Antibodies were also detected for some putative proteins, noticeably the C‐termini of SARS3a and SARS6.</p></sec><sec><title>Conclusions</title><p>Important epitopes of the SARS‐CoV genome that may serve as potential markers for the viral infection are identified. These specific antigenic sites may also be important for vaccine development against this new fatal infectious disease.</p></sec></abstract><kwd-group><kwd>SARS</kwd><kwd>coronavirus</kwd><kwd>antibody</kwd><kwd>peptide chip</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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