Genome sequence variation analysis of two SARS coronavirus isolates after passage in Vero cell culture
Identifieur interne : 001656 ( Pmc/Checkpoint ); précédent : 001655; suivant : 001657Genome sequence variation analysis of two SARS coronavirus isolates after passage in Vero cell culture
Auteurs : Weiwu Jin [République populaire de Chine] ; Liangxiang Hu [République populaire de Chine] ; Zhenglin Du [République populaire de Chine] ; Qiang Gao [République populaire de Chine] ; Hong Gao [République populaire de Chine] ; Ye Ning [République populaire de Chine] ; Jidong Feng [République populaire de Chine] ; Jiansan Zhang [République populaire de Chine] ; Weidong Yin [République populaire de Chine] ; Ning Li [République populaire de Chine]Source :
- Chinese Science Bulletin = Kexue Tongbao [ 1001-6538 ] ; 2004.
Abstract
SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new viral strains during the passage or the transmission. Lots of variations have been detected among different SARS-CoV strains. And a study on these variations is helpful for development of efficient vaccine. Moreover, the test of nucleic acid characterization and genetic stability of SARS-CoV is important in the research of inactivated vaccine. The whole genome sequences of two SARS coronavirus strains after passage in Vero cell culture were determined and were compared with those of early passages, respectively. Results showed that both SARS coronavirus strains have high genetic stability, although nearly 10 generations were passed. Four nucleotide variations were observed between the second passage and the 11th passage of Sino1 strain for identification of SARS inactivated vaccine. Moreover, only one nucleotide was different between the third passage and the 10th passage of Sino3 strain for SARS inactivated vaccine. Therefore, this study suggested it was possible to develop inactivated vaccine against SARS-CoV in the future.
Url:
DOI: 10.1007/BF03183408
PubMed: 32214713
PubMed Central: 7089035
Affiliations:
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Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. 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Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ning, Ye" sort="Ning, Ye" uniqKey="Ning Y" first="Ye" last="Ning">Ye Ning</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Feng, Jidong" sort="Feng, Jidong" uniqKey="Feng J" first="Jidong" last="Feng">Jidong Feng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.22935.3f</institution-id><institution-id institution-id-type="ISNI">0000000405308290</institution-id><institution>State Key Laboratory for Agrobiotechnology,</institution><institution>China Agricultural University,</institution></institution-wrap>100094 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100094 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Jiansan" sort="Zhang, Jiansan" uniqKey="Zhang J" first="Jiansan" last="Zhang">Jiansan Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yin, Weidong" sort="Yin, Weidong" uniqKey="Yin W" first="Weidong" last="Yin">Weidong Yin</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Ning" sort="Li, Ning" uniqKey="Li N" first="Ning" last="Li">Ning Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.22935.3f</institution-id><institution-id institution-id-type="ISNI">0000000405308290</institution-id><institution>State Key Laboratory for Agrobiotechnology,</institution><institution>China Agricultural University,</institution></institution-wrap>100094 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100094 Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">Chinese Science Bulletin = Kexue Tongbao</title><idno type="ISSN">1001-6538</idno><idno type="eISSN">1861-9541</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new viral strains during the passage or the transmission. Lots of variations have been detected among different SARS-CoV strains. And a study on these variations is helpful for development of efficient vaccine. Moreover, the test of nucleic acid characterization and genetic stability of SARS-CoV is important in the research of inactivated vaccine. The whole genome sequences of two SARS coronavirus strains after passage in Vero cell culture were determined and were compared with those of early passages, respectively. Results showed that both SARS coronavirus strains have high genetic stability, although nearly 10 generations were passed. Four nucleotide variations were observed between the second passage and the 11th passage of Sino1 strain for identification of SARS inactivated vaccine. Moreover, only one nucleotide was different between the third passage and the 10th passage of Sino3 strain for SARS inactivated vaccine. Therefore, this study suggested it was possible to develop inactivated vaccine against SARS-CoV in the future.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P. A. Rota</name></author><author><name sortKey="Oberste, S M" uniqKey="Oberste S">S. M. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S. S. Monroe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M. A. Marra</name></author><author><name sortKey="Jones, S J" uniqKey="Jones S">S. J. Jones</name></author><author><name sortKey="Astell, C R" uniqKey="Astell C">C. R. Astell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E. D. Qin</name></author><author><name sortKey="Zhu, Q Y" uniqKey="Zhu Q">Q. Y. Zhu</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Chin Sci Bull</journal-id><journal-id journal-id-type="iso-abbrev">Chin. Sci. Bull</journal-id><journal-title-group><journal-title>Chinese Science Bulletin = Kexue Tongbao</journal-title></journal-title-group><issn pub-type="ppub">1001-6538</issn><issn pub-type="epub">1861-9541</issn><publisher><publisher-name>Science in China Press</publisher-name><publisher-loc>Beijing</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32214713</article-id><article-id pub-id-type="pmc">7089035</article-id><article-id pub-id-type="publisher-id">BF03183408</article-id><article-id pub-id-type="doi">10.1007/BF03183408</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Genome sequence variation analysis of two SARS coronavirus isolates after passage in Vero cell culture</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jin</surname><given-names>Weiwu</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Liangxiang</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Du</surname><given-names>Zhenglin</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Gao</surname><given-names>Qiang</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Gao</surname><given-names>Hong</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ning</surname><given-names>Ye</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Feng</surname><given-names>Jidong</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Jiansan</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Yin</surname><given-names>Weidong</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Li</surname><given-names>Ning</given-names></name><address><email>ninglbau@public3.bta.net.cn</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.22935.3f</institution-id><institution-id institution-id-type="ISNI">0000000405308290</institution-id><institution>State Key Laboratory for Agrobiotechnology,</institution><institution>China Agricultural University,</institution></institution-wrap>100094 Beijing, China</aff><aff id="Aff2"><label>2</label>Sino Biotech Co., Ltd., No. 39 Shangdi Xi Rd. Haidian District, 100085 Beijing, China</aff></contrib-group><pub-date pub-type="ppub"><year>2004</year></pub-date><volume>49</volume><issue>17</issue><fpage>1824</fpage><lpage>1827</lpage><history><date date-type="received"><day>15</day><month>5</month><year>2004</year></date><date date-type="accepted"><day>24</day><month>7</month><year>2004</year></date></history><permissions><copyright-statement>© Science in China Press 2004</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new viral strains during the passage or the transmission. Lots of variations have been detected among different SARS-CoV strains. And a study on these variations is helpful for development of efficient vaccine. Moreover, the test of nucleic acid characterization and genetic stability of SARS-CoV is important in the research of inactivated vaccine. The whole genome sequences of two SARS coronavirus strains after passage in Vero cell culture were determined and were compared with those of early passages, respectively. Results showed that both SARS coronavirus strains have high genetic stability, although nearly 10 generations were passed. Four nucleotide variations were observed between the second passage and the 11th passage of Sino1 strain for identification of SARS inactivated vaccine. Moreover, only one nucleotide was different between the third passage and the 10th passage of Sino3 strain for SARS inactivated vaccine. Therefore, this study suggested it was possible to develop inactivated vaccine against SARS-CoV in the future.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>severe acute respiratory syndrome (SARS)</kwd><kwd>SARS Coronavirus (SARS-CoV)</kwd><kwd>virus</kwd><kwd>vaccine</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Science in China Press 2004</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Jin, Weiwu" sort="Jin, Weiwu" uniqKey="Jin W" first="Weiwu" last="Jin">Weiwu Jin</name></noRegion><name sortKey="Du, Zhenglin" sort="Du, Zhenglin" uniqKey="Du Z" first="Zhenglin" last="Du">Zhenglin Du</name><name sortKey="Feng, Jidong" sort="Feng, Jidong" uniqKey="Feng J" first="Jidong" last="Feng">Jidong Feng</name><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><name sortKey="Gao, Qiang" sort="Gao, Qiang" uniqKey="Gao Q" first="Qiang" last="Gao">Qiang Gao</name><name sortKey="Hu, Liangxiang" sort="Hu, Liangxiang" uniqKey="Hu L" first="Liangxiang" last="Hu">Liangxiang Hu</name><name sortKey="Li, Ning" sort="Li, Ning" uniqKey="Li N" first="Ning" last="Li">Ning Li</name><name sortKey="Ning, Ye" sort="Ning, Ye" uniqKey="Ning Y" first="Ye" last="Ning">Ye Ning</name><name sortKey="Yin, Weidong" sort="Yin, Weidong" uniqKey="Yin W" first="Weidong" last="Yin">Weidong Yin</name><name sortKey="Zhang, Jiansan" sort="Zhang, Jiansan" uniqKey="Zhang J" first="Jiansan" last="Zhang">Jiansan Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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