Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies
Identifieur interne : 001639 ( Pmc/Checkpoint ); précédent : 001638; suivant : 001640Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies
Auteurs : Hong Zhang [République populaire de Chine] ; Guangwen Wang [République populaire de Chine] ; Jian Li [République populaire de Chine] ; Yuchun Nie [République populaire de Chine] ; Xuanling Shi [République populaire de Chine] ; Gewei Lian [République populaire de Chine] ; Wei Wang [République populaire de Chine] ; Xiaolei Yin [République populaire de Chine] ; Yang Zhao [République populaire de Chine] ; Xiuxia Qu [République populaire de Chine] ; Mingxiao Ding [République populaire de Chine] ; Hongkui Deng [République populaire de Chine]Source :
- Journal of Virology [ 0022-538X ] ; 2004.
Abstract
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.
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DOI: 10.1128/JVI.78.13.6938-6945.2004
PubMed: 15194770
PubMed Central: 421668
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies</title><author><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region 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and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life 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Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life 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Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15194770</idno><idno type="pmc">421668</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC421668</idno><idno type="RBID">PMC:421668</idno><idno type="doi">10.1128/JVI.78.13.6938-6945.2004</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000681</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000681</idno><idno type="wicri:Area/Pmc/Curation">000681</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000681</idno><idno type="wicri:Area/Pmc/Checkpoint">001639</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001639</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies</title><author><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Li, Jian" sort="Li, Jian" uniqKey="Li J" first="Jian" last="Li">Jian Li</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Lian, Gewei" sort="Lian, Gewei" uniqKey="Lian G" first="Gewei" last="Lian">Gewei Lian</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Zhao, Yang" sort="Zhao, Yang" uniqKey="Zhao Y" first="Yang" last="Zhao">Yang Zhao</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><affiliation wicri:level="4"><nlm:aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-id journal-id-type="publisher-id">jvi</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15194770</article-id><article-id pub-id-type="pmc">421668</article-id><article-id pub-id-type="publisher-id">2526-03</article-id><article-id pub-id-type="doi">10.1128/JVI.78.13.6938-6945.2004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Vaccines and Antiviral Agents</subject></subj-group></article-categories><title-group><article-title>Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Hong</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="fn" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Guangwen</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="fn" rid="fn1">†</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Jian</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Nie</surname><given-names>Yuchun</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Shi</surname><given-names>Xuanling</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Lian</surname><given-names>Gewei</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Wei</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Yin</surname><given-names>Xiaolei</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Yang</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Qu</surname><given-names>Xiuxia</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Ding</surname><given-names>Mingxiao</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Hongkui</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff0">Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address for H. Deng: Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China. Phone: 86-10-6275-6954. Fax: 86-10-6275-6954. E-mail: <email>hongkui_deng@pku.edu.cn</email>. Phone for M. Ding: 86-10-6276-7047. Fax: 86-10-6275-6954. E-mail: <email>dingmx01@pku.edu.cn</email>.</p></fn><fn id="fn1"><label>†</label><p>H.Z. and G.W. contributed equally to this article.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2004</year></pub-date><volume>78</volume><issue>13</issue><fpage>6938</fpage><lpage>6945</lpage><history><date date-type="received"><day>1</day><month>12</month><year>2003</year></date><date date-type="accepted"><day>26</day><month>2</month><year>2004</year></date></history><copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement><copyright-year>2004</copyright-year><abstract><p>Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.</p></abstract></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name></noRegion><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name><name sortKey="Li, Jian" sort="Li, Jian" uniqKey="Li J" first="Jian" last="Li">Jian Li</name><name sortKey="Lian, Gewei" sort="Lian, Gewei" uniqKey="Lian G" first="Gewei" last="Lian">Gewei Lian</name><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name><name sortKey="Zhao, Yang" sort="Zhao, Yang" uniqKey="Zhao Y" first="Yang" last="Zhao">Yang Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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