Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus
Identifieur interne : 001637 ( Pmc/Checkpoint ); précédent : 001636; suivant : 001638Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus
Auteurs : Liqun Lu ; Ivanus Manopo ; Bernard P. Leung ; Hiok Hee Chng ; Ai Ee Ling ; Li Lian Chee ; Eng Eong Ooi ; Shzu-Wei Chan ; Jimmy KwangSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2004.
Abstract
Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.
Url:
DOI: 10.1128/JCM.42.4.1570-1576.2004
PubMed: 15071006
PubMed Central: 387621
Affiliations:
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Leung</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chng, Hiok Hee" sort="Chng, Hiok Hee" uniqKey="Chng H" first="Hiok Hee" last="Chng">Hiok Hee Chng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai Ee" last="Ling">Ai Ee Ling</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chee, Li Lian" sort="Chee, Li Lian" uniqKey="Chee L" first="Li Lian" last="Chee">Li Lian Chee</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng Eong" last="Ooi">Eng Eong Ooi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chan, Shzu Wei" sort="Chan, Shzu Wei" uniqKey="Chan S" first="Shzu-Wei" last="Chan">Shzu-Wei Chan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15071006</idno><idno type="pmc">387621</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387621</idno><idno type="RBID">PMC:387621</idno><idno type="doi">10.1128/JCM.42.4.1570-1576.2004</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000644</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000644</idno><idno type="wicri:Area/Pmc/Curation">000644</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000644</idno><idno type="wicri:Area/Pmc/Checkpoint">001637</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001637</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus</title><author><name sortKey="Lu, Liqun" sort="Lu, Liqun" uniqKey="Lu L" first="Liqun" last="Lu">Liqun Lu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Manopo, Ivanus" sort="Manopo, Ivanus" uniqKey="Manopo I" first="Ivanus" last="Manopo">Ivanus Manopo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Leung, Bernard P" sort="Leung, Bernard P" uniqKey="Leung B" first="Bernard P." last="Leung">Bernard P. Leung</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chng, Hiok Hee" sort="Chng, Hiok Hee" uniqKey="Chng H" first="Hiok Hee" last="Chng">Hiok Hee Chng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai Ee" last="Ling">Ai Ee Ling</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chee, Li Lian" sort="Chee, Li Lian" uniqKey="Chee L" first="Li Lian" last="Chee">Li Lian Chee</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng Eong" last="Ooi">Eng Eong Ooi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chan, Shzu Wei" sort="Chan, Shzu Wei" uniqKey="Chan S" first="Shzu-Wei" last="Chan">Shzu-Wei Chan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-id journal-id-type="publisher-id">jcm</journal-id><journal-title>Journal of Clinical Microbiology</journal-title><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15071006</article-id><article-id pub-id-type="pmc">387621</article-id><article-id pub-id-type="publisher-id">2012</article-id><article-id pub-id-type="doi">10.1128/JCM.42.4.1570-1576.2004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Virology</subject></subj-group></article-categories><title-group><article-title>Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Liqun</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Manopo</surname><given-names>Ivanus</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Leung</surname><given-names>Bernard P.</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Chng</surname><given-names>Hiok Hee</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Ling</surname><given-names>Ai Ee</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Chee</surname><given-names>Li Lian</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ooi</surname><given-names>Eng Eong</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Shzu-Wei</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kwang</surname><given-names>Jimmy</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">Animal Health Biotechnology Unit, Temasek Life Science Laboratory, National University of Singapore,<label>1</label> Department of Rheumatology, Allergy, and Immunology, Tan Tock Seng Hospital,<label>2</label> Virology Section, Department of Pathology, Singapore General Hospital,<label>3</label> Environment Health Institute, National Environment Agency, Singapore<label>4</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Animal Health Biotechnology Unit, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore. Phone: 65-68727473. Fax: 65-68727007. E-mail: <email>kwang@tll.org.sg</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2004</year></pub-date><volume>42</volume><issue>4</issue><fpage>1570</fpage><lpage>1576</lpage><history><date date-type="received"><day>24</day><month>11</month><year>2003</year></date><date date-type="rev-recd"><day>1</day><month>1</month><year>2004</year></date><date date-type="accepted"><day>7</day><month>1</month><year>2004</year></date></history><copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement><copyright-year>2004</copyright-year><abstract><p>Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.</p></abstract></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chan, Shzu Wei" sort="Chan, Shzu Wei" uniqKey="Chan S" first="Shzu-Wei" last="Chan">Shzu-Wei Chan</name><name sortKey="Chee, Li Lian" sort="Chee, Li Lian" uniqKey="Chee L" first="Li Lian" last="Chee">Li Lian Chee</name><name sortKey="Chng, Hiok Hee" sort="Chng, Hiok Hee" uniqKey="Chng H" first="Hiok Hee" last="Chng">Hiok Hee Chng</name><name sortKey="Kwang, Jimmy" sort="Kwang, Jimmy" uniqKey="Kwang J" first="Jimmy" last="Kwang">Jimmy Kwang</name><name sortKey="Leung, Bernard P" sort="Leung, Bernard P" uniqKey="Leung B" first="Bernard P." last="Leung">Bernard P. Leung</name><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai Ee" last="Ling">Ai Ee Ling</name><name sortKey="Lu, Liqun" sort="Lu, Liqun" uniqKey="Lu L" first="Liqun" last="Lu">Liqun Lu</name><name sortKey="Manopo, Ivanus" sort="Manopo, Ivanus" uniqKey="Manopo I" first="Ivanus" last="Manopo">Ivanus Manopo</name><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng Eong" last="Ooi">Eng Eong Ooi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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