SrasV1, Pmc, Checkpoint, bibRecord, 001360

Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets

Identifieur interne : 001360 ( Pmc/Checkpoint ); précédent : 001359; suivant : 001361

Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets

Auteurs : Wenqian Hu ; Bingke Bai ; Zhihong Hu ; Ze Chen ; Xuefang An ; Lijun Tang ; Jihong Yang ; Hualin Wang ; Hanzhong Wang

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2005.

RBID : PMC:1153763

Abstract

Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10¹ to 10⁶ copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153763

DOI: 10.1128/JCM.43.5.2041-2046.2005
PubMed: 15872219
PubMed Central: 1153763

Affiliations:

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PMC:1153763

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<author><name sortKey="An, Xuefang" sort="An, Xuefang" uniqKey="An X" first="Xuefang" last="An">Xuefang An</name>
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<author><name sortKey="Tang, Lijun" sort="Tang, Lijun" uniqKey="Tang L" first="Lijun" last="Tang">Lijun Tang</name>
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<author><name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name>
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<series><title level="j">Journal of Clinical Microbiology</title>
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<front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup>
 to 10<sup>6</sup>
 copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</p>
</div>
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</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">jcm</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15872219</article-id>
<article-id pub-id-type="pmc">1153763</article-id>
<article-id pub-id-type="publisher-id">1736-04</article-id>
<article-id pub-id-type="doi">10.1128/JCM.43.5.2041-2046.2005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Virology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>Wenqian</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bai</surname>
<given-names>Bingke</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>Zhihong</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Ze</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>An</surname>
<given-names>Xuefang</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tang</surname>
<given-names>Lijun</given-names>
</name>
<xref ref-type="aff" rid="aff1">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Jihong</given-names>
</name>
<xref ref-type="aff" rid="aff1">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Hualin</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Hanzhong</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences,<label>1</label>
 Hubei Center for Diseases Control and Prevention,<label>2</label>
 Wuhan Center for Diseases Control and Prevention, Wuhan, Hubei, People's Republic of China<label>3</label>
</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author. Mailing address: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430072 Hubei, People's Republic of China. Phone: 86-27-87199239. Fax: 86-27-87198072. E-mail: <email>wanghz@pentium.whiov.ac.cn</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>5</month>
<year>2005</year>
</pub-date>
<volume>43</volume>
<issue>5</issue>
<fpage>2041</fpage>
<lpage>2046</lpage>
<history><date date-type="received"><day>18</day>
<month>9</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>5</day>
<month>12</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>14</day>
<month>1</month>
<year>2005</year>
</date>
</history>
<copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement>
<copyright-year>2005</copyright-year>
<abstract><p>Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup>
 to 10<sup>6</sup>
 copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="An, Xuefang" sort="An, Xuefang" uniqKey="An X" first="Xuefang" last="An">Xuefang An</name>
<name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name>
<name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name>
<name sortKey="Hu, Wenqian" sort="Hu, Wenqian" uniqKey="Hu W" first="Wenqian" last="Hu">Wenqian Hu</name>
<name sortKey="Hu, Zhihong" sort="Hu, Zhihong" uniqKey="Hu Z" first="Zhihong" last="Hu">Zhihong Hu</name>
<name sortKey="Tang, Lijun" sort="Tang, Lijun" uniqKey="Tang L" first="Lijun" last="Tang">Lijun Tang</name>
<name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name>
<name sortKey="Wang, Hualin" sort="Wang, Hualin" uniqKey="Wang H" first="Hualin" last="Wang">Hualin Wang</name>
<name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name>
</noCountry>
</tree>
</affiliations>
</record>

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Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets

Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets

Source :

Abstract

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