First external quality assurance of antibody diagnostic for SARS-new coronavirus
Identifieur interne : 001336 ( Pmc/Checkpoint ); précédent : 001335; suivant : 001337First external quality assurance of antibody diagnostic for SARS-new coronavirus
Auteurs : Matthias Niedrig [Allemagne] ; Katrin Leitmeyer [Allemagne, Suisse] ; Wilina Lim [République populaire de Chine] ; Malik Peiris [République populaire de Chine] ; John S. Mackenzie [Suisse, Australie] ; Maria Zambon [Royaume-Uni]Source :
- Journal of Clinical Virology [ 1386-6532 ] ; 2005.
Abstract
To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.
Url:
DOI: 10.1016/j.jcv.2005.01.004
PubMed: 16087120
PubMed Central: 7108360
Affiliations:
- Allemagne, Australie, Royaume-Uni, République populaire de Chine, Suisse
- Angleterre, Berlin, Grand Londres
- Berlin, Londres
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To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chang, M S" uniqKey="Chang M">M.S. Chang</name></author><author><name sortKey="Lu, Y T" uniqKey="Lu Y">Y.T. Lu</name></author><author><name sortKey="Ho, S T" uniqKey="Ho S">S.T. Ho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, J P" uniqKey="Guo J">J.P. Guo</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M. Petric</name></author><author><name sortKey="Campbell, W" uniqKey="Campbell W">W. Campbell</name></author><author><name sortKey="Mcgeer, P L" uniqKey="Mcgeer P">P.L. McGeer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Q" uniqKey="He Q">Q. He</name></author><author><name sortKey="Chong, K H" uniqKey="Chong K">K.H. Chong</name></author><author><name sortKey="Chng, H H" uniqKey="Chng H">H.H. Chng</name></author><author><name sortKey="Leung, B" uniqKey="Leung B">B. Leung</name></author><author><name sortKey="Ling, A E" uniqKey="Ling A">A.E. Ling</name></author><author><name sortKey="Wei, T" uniqKey="Wei T">T. Wei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heymann, D L" uniqKey="Heymann D">D.L. Heymann</name></author><author><name sortKey="Aylward, R B" uniqKey="Aylward R">R.B. Aylward</name></author><author><name sortKey="Wolff, C" uniqKey="Wolff C">C. Wolff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D. Erdman</name></author><author><name sortKey="Goldsmith, C S" uniqKey="Goldsmith C">C.S. Goldsmith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M. Schutten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. Lai</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M. Chu</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C. Cheng</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Wu, H S" uniqKey="Wu H">H.S. Wu</name></author><author><name sortKey="Chiu, S C" uniqKey="Chiu S">S.C. Chiu</name></author><author><name sortKey="Tseng, T C" uniqKey="Tseng T">T.C. Tseng</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Virol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Virol</journal-id><journal-title-group><journal-title>Journal of Clinical Virology</journal-title></journal-title-group><issn pub-type="ppub">1386-6532</issn><issn pub-type="epub">1873-5967</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16087120</article-id><article-id pub-id-type="pmc">7108360</article-id><article-id pub-id-type="publisher-id">S1386-6532(05)00032-6</article-id><article-id pub-id-type="doi">10.1016/j.jcv.2005.01.004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>First external quality assurance of antibody diagnostic for SARS-new coronavirus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Niedrig</surname><given-names>Matthias</given-names></name><email>niedrigm@rki.de</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Leitmeyer</surname><given-names>Katrin</given-names></name><email>leitmeyerk@rki.de</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Lim</surname><given-names>Wilina</given-names></name><email>wllim@pacific.net.hk</email><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Peiris</surname><given-names>Malik</given-names></name><email>malik@hkucc.hku.hk</email><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Mackenzie</surname><given-names>John S.</given-names></name><email>j.mackenzie@curtin.edu.au</email><xref rid="aff2" ref-type="aff">b</xref><xref rid="aff5" ref-type="aff">e</xref></contrib><contrib contrib-type="author"><name><surname>Zambon</surname><given-names>Maria</given-names></name><email>maria.zambon@hpa.org.uk</email><xref rid="aff6" ref-type="aff">f</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany</aff><aff id="aff2"><label>b</label>World Health Organization, Geneva, Switzerland</aff><aff id="aff3"><label>c</label>Government Virus Unit Hong Kong, Hong Kong, China</aff><aff id="aff4"><label>d</label>Department of Microbiology, The University of Hong Kong, Hong Kong, China</aff><aff id="aff5"><label>e</label>Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Australia</aff><aff id="aff6"><label>f</label>Health Protection Agency, London, UK</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Tel.: +49 30 4547 2370/2321; fax: +49 30 4547 2321/2390. <email>niedrigm@rki.de</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>15</day><month>2</month><year>2005</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>15</day><month>2</month><year>2005</year></pub-date><volume>34</volume><issue>1</issue><fpage>22</fpage><lpage>25</lpage><history><date date-type="received"><day>23</day><month>12</month><year>2004</year></date><date date-type="rev-recd"><day>17</day><month>1</month><year>2005</year></date><date date-type="accepted"><day>19</day><month>1</month><year>2005</year></date></history><permissions><copyright-statement>Copyright © 2005 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2005</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>To confirm an infection with the new coronavirus (SARS-CoV) causing the severe acute respiratory syndrome (SARS) diagnostic assays for detection of SARS-CoV specific antibody are necessary. To evaluate the diagnostic performance of laboratories an external quality assurance (EQA) study was performed in 2004. Participating laboratories (9/20) correctly detected anti-SARS antibodies in serum samples without false positive results in an immunofluorescence assay. In contrast, only 4/13 laboratories detected most of the anti-SARS antibody positive samples without false positive results using enzyme immunoassays (EIA) and/or immunoblot. The overall results clearly demonstrate that serological diagnosis of SARS-CoV remains at an early stage of development, with further technical improvements required, particularly with respect to the use of SARS specific EIAs.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>Antibody diagnostic</kwd><kwd>External quality assurance</kwd><kwd>EQA</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>Royaume-Uni</li><li>République populaire de Chine</li><li>Suisse</li></country><region><li>Angleterre</li><li>Berlin</li><li>Grand Londres</li></region><settlement><li>Berlin</li><li>Londres</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name></region><name sortKey="Leitmeyer, Katrin" sort="Leitmeyer, Katrin" uniqKey="Leitmeyer K" first="Katrin" last="Leitmeyer">Katrin Leitmeyer</name></country><country name="Suisse"><noRegion><name sortKey="Leitmeyer, Katrin" sort="Leitmeyer, Katrin" uniqKey="Leitmeyer K" first="Katrin" last="Leitmeyer">Katrin Leitmeyer</name></noRegion><name sortKey="Mackenzie, John S" sort="Mackenzie, John S" uniqKey="Mackenzie J" first="John S." last="Mackenzie">John S. Mackenzie</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Lim, Wilina" sort="Lim, Wilina" uniqKey="Lim W" first="Wilina" last="Lim">Wilina Lim</name></noRegion><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name></country><country name="Australie"><noRegion><name sortKey="Mackenzie, John S" sort="Mackenzie, John S" uniqKey="Mackenzie J" first="John S." last="Mackenzie">John S. Mackenzie</name></noRegion></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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