Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes
Identifieur interne : 001305 ( Pmc/Checkpoint ); précédent : 001304; suivant : 001306Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes
Auteurs : Nigel J. Temperton [Royaume-Uni] ; Paul K. Chan [République populaire de Chine] ; Graham Simmons [États-Unis] ; Maria C. Zambon [Royaume-Uni] ; Richard S. Tedder [Royaume-Uni] ; Yasuhiro Takeuchi [Royaume-Uni] ; Robin A. Weiss [Royaume-Uni]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2005.
Abstract
SARS-CoV spike protein pseudotypes are the basis of an in vitro microneutralization assay sensitive and specific for SARS-CoV neutralizing antibodies.
Url:
DOI: 10.3201/eid1103.040906
PubMed: 15757556
PubMed Central: 3298259
Affiliations:
- Royaume-Uni, République populaire de Chine, États-Unis
- Angleterre, Grand Londres, Pennsylvanie
- Londres
- University College de Londres
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Temperton</name><affiliation wicri:level="4"><nlm:aff id="aff1">University College London, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author><author><name sortKey="Chan, Paul K" sort="Chan, Paul K" uniqKey="Chan P" first="Paul K." last="Chan">Paul K. Chan</name><affiliation wicri:level="1"><nlm:aff id="aff2">Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Simmons, Graham" sort="Simmons, Graham" uniqKey="Simmons G" first="Graham" last="Simmons">Graham Simmons</name><affiliation wicri:level="2"><nlm:aff id="aff3">University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Zambon, Maria C" sort="Zambon, Maria C" uniqKey="Zambon M" first="Maria C." last="Zambon">Maria C. 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Weiss</name><affiliation wicri:level="4"><nlm:aff id="aff1">University College London, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>SARS-CoV spike protein pseudotypes are the basis of an in vitro microneutralization assay sensitive and specific for SARS-CoV neutralizing antibodies.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Emerging Infect. Dis</journal-id><journal-id journal-id-type="publisher-id">EID</journal-id><journal-title-group><journal-title>Emerging Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">1080-6040</issn><issn pub-type="epub">1080-6059</issn><publisher><publisher-name>Centers for Disease Control and Prevention</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15757556</article-id><article-id pub-id-type="pmc">3298259</article-id><article-id pub-id-type="publisher-id">04-0906</article-id><article-id pub-id-type="doi">10.3201/eid1103.040906</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes</article-title><alt-title alt-title-type="running-head">Neutralizing Antibody Response to SARS Coronavirus</alt-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Temperton</surname><given-names>Nigel J.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Paul K.</given-names></name><xref ref-type="aff" rid="aff2">†</xref></contrib><contrib contrib-type="author"><name><surname>Simmons</surname><given-names>Graham</given-names></name><xref ref-type="aff" rid="aff3">‡</xref></contrib><contrib contrib-type="author"><name><surname>Zambon</surname><given-names>Maria C.</given-names></name><xref ref-type="aff" rid="aff4">§</xref></contrib><contrib contrib-type="author"><name><surname>Tedder</surname><given-names>Richard S.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Takeuchi</surname><given-names>Yasuhiro</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Weiss</surname><given-names>Robin A.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><aff id="aff1"><label>*</label>University College London, London, United Kingdom</aff><aff id="aff2"><label>†</label>Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People’s Republic of China</aff><aff id="aff3"><label>‡</label>University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA</aff><aff id="aff4"><label>§</label>Health Protection Agency Central Public Health Laboratory, London, United Kingdom</aff></contrib-group><author-notes><corresp id="cor1">Address for correspondence: Nigel Temperton, Wohl Virion Centre and Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, United Kingdom; fax: +44-2076799555; email: <email xlink:href="nigel.temperton@ucl.ac.uk">nigel.temperton@ucl.ac.uk</email></corresp></author-notes><pub-date pub-type="ppub"><month>3</month><year>2005</year></pub-date><volume>11</volume><issue>3</issue><fpage>411</fpage><lpage>416</lpage><abstract abstract-type="toc"><p>SARS-CoV spike protein pseudotypes are the basis of an in vitro microneutralization assay sensitive and specific for SARS-CoV neutralizing antibodies.</p></abstract><abstract><p>The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines.</p></abstract><kwd-group kwd-group-type="author"><title>Keywords: </title><kwd>SARS virus</kwd><kwd>Neutralization tests</kwd><kwd>Infectious diseases</kwd><kwd>emerging</kwd><kwd>Vaccines</kwd><kwd>research</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Royaume-Uni</li><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Grand Londres</li><li>Pennsylvanie</li></region><settlement><li>Londres</li></settlement><orgName><li>University College de Londres</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Temperton, Nigel J" sort="Temperton, Nigel J" uniqKey="Temperton N" first="Nigel J." last="Temperton">Nigel J. Temperton</name></region><name sortKey="Takeuchi, Yasuhiro" sort="Takeuchi, Yasuhiro" uniqKey="Takeuchi Y" first="Yasuhiro" last="Takeuchi">Yasuhiro Takeuchi</name><name sortKey="Tedder, Richard S" sort="Tedder, Richard S" uniqKey="Tedder R" first="Richard S." last="Tedder">Richard S. Tedder</name><name sortKey="Weiss, Robin A" sort="Weiss, Robin A" uniqKey="Weiss R" first="Robin A." last="Weiss">Robin A. Weiss</name><name sortKey="Zambon, Maria C" sort="Zambon, Maria C" uniqKey="Zambon M" first="Maria C." last="Zambon">Maria C. Zambon</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Paul K" sort="Chan, Paul K" uniqKey="Chan P" first="Paul K." last="Chan">Paul K. Chan</name></noRegion></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Simmons, Graham" sort="Simmons, Graham" uniqKey="Simmons G" first="Graham" last="Simmons">Graham Simmons</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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