Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
Identifieur interne : 001114 ( Pmc/Checkpoint ); précédent : 001113; suivant : 001115Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
Auteurs : Chao Qian ; Di Qin ; Qiao Tang ; Yi Zeng ; Guixia Tang ; Chun LuSource :
- Virus Genes [ 0920-8569 ] ; 2006.
Abstract
To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in
Url:
DOI: 10.1007/s11262-005-0050-8
PubMed: 16972028
PubMed Central: 7088559
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein</title><author><name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16972028</idno><idno type="pmc">7088559</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088559</idno><idno type="RBID">PMC:7088559</idno><idno type="doi">10.1007/s11262-005-0050-8</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">000504</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000504</idno><idno type="wicri:Area/Pmc/Curation">000504</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000504</idno><idno type="wicri:Area/Pmc/Checkpoint">001114</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001114</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein</title><author><name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in <italic>Escherichia coli</italic> (<italic>E. coli</italic>). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Genes</journal-id><journal-id journal-id-type="iso-abbrev">Virus Genes</journal-id><journal-title-group><journal-title>Virus Genes</journal-title></journal-title-group><issn pub-type="ppub">0920-8569</issn><issn pub-type="epub">1572-994X</issn><publisher><publisher-name>Springer US</publisher-name><publisher-loc>New York</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16972028</article-id><article-id pub-id-type="pmc">7088559</article-id><article-id pub-id-type="publisher-id">50</article-id><article-id pub-id-type="doi">10.1007/s11262-005-0050-8</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Qian</surname><given-names>Chao</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Qin</surname><given-names>Di</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Qiao</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Guixia</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Lu</surname><given-names>Chun</given-names></name><address><email>clu@njmu.edu.cn</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.89957.3a</institution-id><institution-id institution-id-type="ISNI">0000000092558984</institution-id><institution>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology,</institution><institution>Nanjing Medical University,</institution></institution-wrap>Nanjing , 210029 P.R. China</aff></contrib-group><pub-date pub-type="epub"><day>1</day><month>10</month><year>2006</year></pub-date><pub-date pub-type="ppub"><year>2006</year></pub-date><volume>33</volume><issue>2</issue><fpage>147</fpage><lpage>156</lpage><history><date date-type="received"><day>26</day><month>10</month><year>2005</year></date><date date-type="accepted"><day>19</day><month>12</month><year>2005</year></date></history><permissions><copyright-statement>© Springer Science+Business Media, LLC 2006</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in <italic>Escherichia coli</italic> (<italic>E. coli</italic>). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>SARS-associated coronavirus</kwd><kwd>Recombinant M protein</kwd><kwd>B-cell antigenic epitope</kwd><kwd>Monoclonal antibody</kwd><kwd>Western blot</kwd><kwd>ELISA</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media, LLC 2006</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name><name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name><name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name><name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name><name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name><name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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