Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice☆
Identifieur interne : 001056 ( Pmc/Checkpoint ); précédent : 001055; suivant : 001057Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice☆
Auteurs : Hua Yu [République populaire de Chine] ; Li-Fang Jiang [République populaire de Chine] ; Dan-Yun Fang [République populaire de Chine] ; Hui-Jun Yan [République populaire de Chine] ; Jing-Jiao Zhou [République populaire de Chine] ; Jun-Mei Zhou [République populaire de Chine] ; Yu Liang [République populaire de Chine] ; Yang Gao [République populaire de Chine] ; Wei Zhao [République populaire de Chine] ; Bei-Guo Long [République populaire de Chine]Source :
- Virology [ 0042-6822 ] ; 2006.
Abstract
Antibodies to
Url:
DOI: 10.1016/j.virol.2006.09.016
PubMed: 17055022
PubMed Central: 7103350
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection of <italic>SARS-Coronavirus</italic>-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice<sup><xref ref-type="fn" rid="d32e2337">☆</xref></sup></title><author><name sortKey="Yu, Hua" sort="Yu, Hua" uniqKey="Yu H" first="Hua" last="Yu">Hua Yu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jiang, Li Fang" sort="Jiang, Li Fang" uniqKey="Jiang L" first="Li-Fang" last="Jiang">Li-Fang Jiang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Fang, Dan Yun" sort="Fang, Dan Yun" uniqKey="Fang D" first="Dan-Yun" last="Fang">Dan-Yun Fang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yan, Hui Jun" sort="Yan, Hui Jun" uniqKey="Yan H" first="Hui-Jun" last="Yan">Hui-Jun Yan</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Jing Jiao" sort="Zhou, Jing Jiao" uniqKey="Zhou J" first="Jing-Jiao" last="Zhou">Jing-Jiao Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Jun Mei" sort="Zhou, Jun Mei" uniqKey="Zhou J" first="Jun-Mei" last="Zhou">Jun-Mei Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liang, Yu" sort="Liang, Yu" uniqKey="Liang Y" first="Yu" last="Liang">Yu Liang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Gao, Yang" sort="Gao, Yang" uniqKey="Gao Y" first="Yang" last="Gao">Yang Gao</name><affiliation wicri:level="1"><nlm:aff id="aff2">Guangzhou City CDC, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou City CDC</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Microbiology, Southern Medical University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Southern Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Long, Bei Guo" sort="Long, Bei Guo" uniqKey="Long B" first="Bei-Guo" last="Long">Bei-Guo Long</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Microbiology, Southern Medical University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Southern Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">17055022</idno><idno type="pmc">7103350</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103350</idno><idno type="RBID">PMC:7103350</idno><idno type="doi">10.1016/j.virol.2006.09.016</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">001553</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001553</idno><idno type="wicri:Area/Pmc/Curation">001553</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001553</idno><idno type="wicri:Area/Pmc/Checkpoint">001056</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001056</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Selection of <italic>SARS-Coronavirus</italic>-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice<sup><xref ref-type="fn" rid="d32e2337">☆</xref></sup></title><author><name sortKey="Yu, Hua" sort="Yu, Hua" uniqKey="Yu H" first="Hua" last="Yu">Hua Yu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jiang, Li Fang" sort="Jiang, Li Fang" uniqKey="Jiang L" first="Li-Fang" last="Jiang">Li-Fang Jiang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Fang, Dan Yun" sort="Fang, Dan Yun" uniqKey="Fang D" first="Dan-Yun" last="Fang">Dan-Yun Fang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yan, Hui Jun" sort="Yan, Hui Jun" uniqKey="Yan H" first="Hui-Jun" last="Yan">Hui-Jun Yan</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Jing Jiao" sort="Zhou, Jing Jiao" uniqKey="Zhou J" first="Jing-Jiao" last="Zhou">Jing-Jiao Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Jun Mei" sort="Zhou, Jun Mei" uniqKey="Zhou J" first="Jun-Mei" last="Zhou">Jun-Mei Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liang, Yu" sort="Liang, Yu" uniqKey="Liang Y" first="Yu" last="Liang">Yu Liang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Gao, Yang" sort="Gao, Yang" uniqKey="Gao Y" first="Yang" last="Gao">Yang Gao</name><affiliation wicri:level="1"><nlm:aff id="aff2">Guangzhou City CDC, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou City CDC</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Microbiology, Southern Medical University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Southern Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Long, Bei Guo" sort="Long, Bei Guo" uniqKey="Long B" first="Bei-Guo" last="Long">Bei-Guo Long</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Microbiology, Southern Medical University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Southern Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><idno type="eISSN">1096-0341</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Antibodies to <italic>SARS-Coronavirus</italic> (<italic>SARS-CoV</italic>)-specific B cell epitopes might recognize the pathogen and interrupt its adherence to and penetration of host cells. Hence, these epitopes could be useful for diagnosis and as vaccine constituents. Using the phage-displayed peptide library screening method and purified Fab fragments of immunoglobulin G (IgG Fab) from normal human sera and convalescent sera from <italic>SARS-CoV</italic>-infected patients as targets, 11 B cell epitopes of <italic>SARS-CoV</italic> spike glycoprotein (S protein) and membrane protein (M protein) were screened. After a bioinformatics tool was used to analyze these epitopes, four epitope-based S protein dodecapeptides corresponding to the predominant epitopes were chosen for synthesis. Their antigenic specificities and immunogenicities were studied <italic>in vitro</italic> and <italic>in vivo.</italic> Flow cytometry and ELISPOT analysis of lymphocytes as well as a serologic analysis of antibody showed that these peptides could trigger a rapid, highly effective, and relatively safe immune response in BALB/c mice. These findings might aid development of SARS diagnostics and vaccines. Moreover, the role of S and M proteins as important surface antigens is confirmed.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adey, N B" uniqKey="Adey N">N.B. Adey</name></author><author><name sortKey="Mataragnon, A H" uniqKey="Mataragnon A">A.H. Mataragnon</name></author><author><name sortKey="Rider, J E" uniqKey="Rider J">J.E. Rider</name></author><author><name sortKey="Carter, J M" uniqKey="Carter J">J.M. Carter</name></author><author><name sortKey="Kay, B K" uniqKey="Kay B">B.K. Kay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akbar, A N" uniqKey="Akbar A">A.N. Akbar</name></author><author><name sortKey="Salmon, M" uniqKey="Salmon M">M. Salmon</name></author><author><name sortKey="Savill, J" uniqKey="Savill J">J. Savill</name></author><author><name sortKey="Janossy, G" uniqKey="Janossy G">G. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Virology</journal-id><journal-id journal-id-type="iso-abbrev">Virology</journal-id><journal-title-group><journal-title>Virology</journal-title></journal-title-group><issn pub-type="ppub">0042-6822</issn><issn pub-type="epub">1096-0341</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">17055022</article-id><article-id pub-id-type="pmc">7103350</article-id><article-id pub-id-type="publisher-id">S0042-6822(06)00669-6</article-id><article-id pub-id-type="doi">10.1016/j.virol.2006.09.016</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Selection of <italic>SARS-Coronavirus</italic>-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice<sup><xref ref-type="fn" rid="d32e2337">☆</xref></sup></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Hua</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Li-Fang</given-names></name><email>jianglf909@yahoo.com.cn</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Fang</surname><given-names>Dan-Yun</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Yan</surname><given-names>Hui-Jun</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Jing-Jiao</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Jun-Mei</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Liang</surname><given-names>Yu</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Gao</surname><given-names>Yang</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Wei</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Long</surname><given-names>Bei-Guo</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Department of Microbiology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhong-shan 2-Road, Guangzhou 510080, China</aff><aff id="aff2"><label>b</label>Guangzhou City CDC, China</aff><aff id="aff3"><label>c</label>Department of Microbiology, Southern Medical University, Guangzhou, China</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author. Fax: +86 20 8734 4779. <email>jianglf909@yahoo.com.cn</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>19</day><month>10</month><year>2006</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>15</day><month>3</month><year>2007</year></pub-date><pub-date pub-type="epub"><day>19</day><month>10</month><year>2006</year></pub-date><volume>359</volume><issue>2</issue><fpage>264</fpage><lpage>274</lpage><history><date date-type="received"><day>17</day><month>5</month><year>2006</year></date><date date-type="rev-recd"><day>13</day><month>9</month><year>2006</year></date><date date-type="accepted"><day>18</day><month>9</month><year>2006</year></date></history><permissions><copyright-statement>Copyright © 2006 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2006</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Antibodies to <italic>SARS-Coronavirus</italic> (<italic>SARS-CoV</italic>)-specific B cell epitopes might recognize the pathogen and interrupt its adherence to and penetration of host cells. Hence, these epitopes could be useful for diagnosis and as vaccine constituents. Using the phage-displayed peptide library screening method and purified Fab fragments of immunoglobulin G (IgG Fab) from normal human sera and convalescent sera from <italic>SARS-CoV</italic>-infected patients as targets, 11 B cell epitopes of <italic>SARS-CoV</italic> spike glycoprotein (S protein) and membrane protein (M protein) were screened. After a bioinformatics tool was used to analyze these epitopes, four epitope-based S protein dodecapeptides corresponding to the predominant epitopes were chosen for synthesis. Their antigenic specificities and immunogenicities were studied <italic>in vitro</italic> and <italic>in vivo.</italic> Flow cytometry and ELISPOT analysis of lymphocytes as well as a serologic analysis of antibody showed that these peptides could trigger a rapid, highly effective, and relatively safe immune response in BALB/c mice. These findings might aid development of SARS diagnostics and vaccines. Moreover, the role of S and M proteins as important surface antigens is confirmed.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>B cell epitope</kwd><kwd>Phage display peptide library</kwd><kwd>Peptide</kwd><kwd>Flow cytometry</kwd><kwd>ELISPOT</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Yu, Hua" sort="Yu, Hua" uniqKey="Yu H" first="Hua" last="Yu">Hua Yu</name></region><name sortKey="Fang, Dan Yun" sort="Fang, Dan Yun" uniqKey="Fang D" first="Dan-Yun" last="Fang">Dan-Yun Fang</name><name sortKey="Gao, Yang" sort="Gao, Yang" uniqKey="Gao Y" first="Yang" last="Gao">Yang Gao</name><name sortKey="Jiang, Li Fang" sort="Jiang, Li Fang" uniqKey="Jiang L" first="Li-Fang" last="Jiang">Li-Fang Jiang</name><name sortKey="Liang, Yu" sort="Liang, Yu" uniqKey="Liang Y" first="Yu" last="Liang">Yu Liang</name><name sortKey="Long, Bei Guo" sort="Long, Bei Guo" uniqKey="Long B" first="Bei-Guo" last="Long">Bei-Guo Long</name><name sortKey="Yan, Hui Jun" sort="Yan, Hui Jun" uniqKey="Yan H" first="Hui-Jun" last="Yan">Hui-Jun Yan</name><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name><name sortKey="Zhou, Jing Jiao" sort="Zhou, Jing Jiao" uniqKey="Zhou J" first="Jing-Jiao" last="Zhou">Jing-Jiao Zhou</name><name sortKey="Zhou, Jun Mei" sort="Zhou, Jun Mei" uniqKey="Zhou J" first="Jun-Mei" last="Zhou">Jun-Mei Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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