A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro
Identifieur interne : 000E60 ( Pmc/Checkpoint ); précédent : 000E59; suivant : 000E61A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro
Auteurs : Xiaohua Wang [République populaire de Chine] ; Wei Xu [République populaire de Chine] ; Deyan Tong [République populaire de Chine] ; Jing Ni [République populaire de Chine] ; Haifeng Gao [République populaire de Chine] ; Ying Wang [République populaire de Chine] ; Yiwei Chu [République populaire de Chine] ; Pingping Li [République populaire de Chine] ; Xiaoming Yang [République populaire de Chine] ; Sidong Xiong [République populaire de Chine]Source :
- Immunology Letters [ 0165-2478 ] ; 2008.
Abstract
Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S437–459 and M1–20. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.
Url:
DOI: 10.1016/j.imlet.2008.04.005
PubMed: 18533276
PubMed Central: 7112888
Affiliations:
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200032</wicri:noRegion></affiliation></author><author><name sortKey="Ni, Jing" sort="Ni, Jing" uniqKey="Ni J" first="Jing" last="Ni">Jing Ni</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Haifeng" sort="Gao, Haifeng" uniqKey="Gao H" first="Haifeng" last="Gao">Haifeng Gao</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Chu, Yiwei" sort="Chu, Yiwei" uniqKey="Chu Y" first="Yiwei" last="Chu">Yiwei Chu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Li, Pingping" sort="Li, Pingping" uniqKey="Li P" first="Pingping" last="Li">Pingping Li</name><affiliation wicri:level="1"><nlm:aff id="aff3">Wuhan Institute of Biological Products, Hubei 430060, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan Institute of Biological Products, Hubei 430060</wicri:regionArea><wicri:noRegion>Hubei 430060</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiaoming" sort="Yang, Xiaoming" uniqKey="Yang X" first="Xiaoming" last="Yang">Xiaoming Yang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Wuhan Institute of Biological Products, Hubei 430060, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan Institute of Biological Products, Hubei 430060</wicri:regionArea><wicri:noRegion>Hubei 430060</wicri:noRegion></affiliation></author><author><name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biomedical Science, Fudan University, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Biomedical Science, Fudan University</wicri:regionArea><wicri:noRegion>Fudan University</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18533276</idno><idno type="pmc">7112888</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112888</idno><idno type="RBID">PMC:7112888</idno><idno type="doi">10.1016/j.imlet.2008.04.005</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">001051</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001051</idno><idno type="wicri:Area/Pmc/Curation">001051</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001051</idno><idno type="wicri:Area/Pmc/Checkpoint">000E60</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000E60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV <italic>in vitro</italic></title><author><name sortKey="Wang, Xiaohua" sort="Wang, Xiaohua" uniqKey="Wang X" first="Xiaohua" last="Wang">Xiaohua Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Wei" sort="Xu, Wei" uniqKey="Xu W" first="Wei" last="Xu">Wei Xu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Tong, Deyan" sort="Tong, Deyan" uniqKey="Tong D" first="Deyan" last="Tong">Deyan Tong</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Ni, Jing" sort="Ni, Jing" uniqKey="Ni J" first="Jing" last="Ni">Jing Ni</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Haifeng" sort="Gao, Haifeng" uniqKey="Gao H" first="Haifeng" last="Gao">Haifeng Gao</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Chu, Yiwei" sort="Chu, Yiwei" uniqKey="Chu Y" first="Yiwei" last="Chu">Yiwei Chu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Li, Pingping" sort="Li, Pingping" uniqKey="Li P" first="Pingping" last="Li">Pingping Li</name><affiliation wicri:level="1"><nlm:aff id="aff3">Wuhan Institute of Biological Products, Hubei 430060, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan Institute of Biological Products, Hubei 430060</wicri:regionArea><wicri:noRegion>Hubei 430060</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiaoming" sort="Yang, Xiaoming" uniqKey="Yang X" first="Xiaoming" last="Yang">Xiaoming Yang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Wuhan Institute of Biological Products, Hubei 430060, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan Institute of Biological Products, Hubei 430060</wicri:regionArea><wicri:noRegion>Hubei 430060</wicri:noRegion></affiliation></author><author><name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biomedical Science, Fudan University, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Biomedical Science, Fudan University</wicri:regionArea><wicri:noRegion>Fudan University</wicri:noRegion></affiliation></author></analytic><series><title level="j">Immunology Letters</title><idno type="ISSN">0165-2478</idno><idno type="eISSN">1879-0542</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S<sub>437–459</sub> and M<sub>1–20</sub>. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Weiss, S R" uniqKey="Weiss S">S.R. Weiss</name></author><author><name sortKey="Navas Martin, S" uniqKey="Navas Martin S">S. Navas-Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, J S" uniqKey="Zhang J">J.S. Zhang</name></author><author><name sortKey="Chen, J T" uniqKey="Chen J">J.T. Chen</name></author><author><name sortKey="Liu, Y X" uniqKey="Liu Y">Y.X. Liu</name></author><author><name sortKey="Zhang, Z S" uniqKey="Zhang Z">Z.S. Zhang</name></author><author><name sortKey="Gao, H" uniqKey="Gao H">H. Gao</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Immunol Lett</journal-id><journal-id journal-id-type="iso-abbrev">Immunol. Lett</journal-id><journal-title-group><journal-title>Immunology Letters</journal-title></journal-title-group><issn pub-type="ppub">0165-2478</issn><issn pub-type="epub">1879-0542</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18533276</article-id><article-id pub-id-type="pmc">7112888</article-id><article-id pub-id-type="publisher-id">S0165-2478(08)00119-3</article-id><article-id pub-id-type="doi">10.1016/j.imlet.2008.04.005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV <italic>in vitro</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xiaohua</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Wei</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Tong</surname><given-names>Deyan</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Ni</surname><given-names>Jing</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Gao</surname><given-names>Haifeng</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Ying</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Chu</surname><given-names>Yiwei</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Pingping</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Xiaoming</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Xiong</surname><given-names>Sidong</given-names></name><email>sdxiongfd@126.com</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</aff><aff id="aff2"><label>b</label>Institute of Biomedical Science, Fudan University, PR China</aff><aff id="aff3"><label>c</label>Wuhan Institute of Biological Products, Hubei 430060, PR China</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author at: Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, PR China. Tel.: +86 21 54237324; fax: +86 21 54237749. <email>sdxiongfd@126.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>19</day><month>5</month><year>2008</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>15</day><month>8</month><year>2008</year></pub-date><pub-date pub-type="epub"><day>19</day><month>5</month><year>2008</year></pub-date><volume>119</volume><issue>1</issue><fpage>71</fpage><lpage>77</lpage><history><date date-type="received"><day>6</day><month>2</month><year>2008</year></date><date date-type="rev-recd"><day>11</day><month>4</month><year>2008</year></date><date date-type="accepted"><day>22</day><month>4</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2008</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S<sub>437–459</sub> and M<sub>1–20</sub>. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Epitope</kwd><kwd>DNA vaccine</kwd><kwd>Antibody</kwd><kwd>Prime–boost</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Xiaohua" sort="Wang, Xiaohua" uniqKey="Wang X" first="Xiaohua" last="Wang">Xiaohua Wang</name></noRegion><name sortKey="Chu, Yiwei" sort="Chu, Yiwei" uniqKey="Chu Y" first="Yiwei" last="Chu">Yiwei Chu</name><name sortKey="Gao, Haifeng" sort="Gao, Haifeng" uniqKey="Gao H" first="Haifeng" last="Gao">Haifeng Gao</name><name sortKey="Li, Pingping" sort="Li, Pingping" uniqKey="Li P" first="Pingping" last="Li">Pingping Li</name><name sortKey="Ni, Jing" sort="Ni, Jing" uniqKey="Ni J" first="Jing" last="Ni">Jing Ni</name><name sortKey="Tong, Deyan" sort="Tong, Deyan" uniqKey="Tong D" first="Deyan" last="Tong">Deyan Tong</name><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name><name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name><name sortKey="Xu, Wei" sort="Xu, Wei" uniqKey="Xu W" first="Wei" last="Xu">Wei Xu</name><name sortKey="Yang, Xiaoming" sort="Yang, Xiaoming" uniqKey="Yang X" first="Xiaoming" last="Yang">Xiaoming Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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