A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro
Identifieur interne : 000E60 ( Pmc/Checkpoint ); précédent : 000E59; suivant : 000E61A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro
Auteurs : Xiaohua Wang [République populaire de Chine] ; Wei Xu [République populaire de Chine] ; Deyan Tong [République populaire de Chine] ; Jing Ni [République populaire de Chine] ; Haifeng Gao [République populaire de Chine] ; Ying Wang [République populaire de Chine] ; Yiwei Chu [République populaire de Chine] ; Pingping Li [République populaire de Chine] ; Xiaoming Yang [République populaire de Chine] ; Sidong Xiong [République populaire de Chine]Source :
- Immunology Letters [ 0165-2478 ] ; 2008.
Abstract
Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S437–459 and M1–20. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.
Url:
DOI: 10.1016/j.imlet.2008.04.005
PubMed: 18533276
PubMed Central: 7112888
Affiliations:
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<series><title level="j">Immunology Letters</title>
<idno type="ISSN">0165-2478</idno>
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<front><div type="abstract" xml:lang="en"><p>Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S<sub>437–459</sub>
and M<sub>1–20</sub>
. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Immunol Lett</journal-id>
<journal-id journal-id-type="iso-abbrev">Immunol. Lett</journal-id>
<journal-title-group><journal-title>Immunology Letters</journal-title>
</journal-title-group>
<issn pub-type="ppub">0165-2478</issn>
<issn pub-type="epub">1879-0542</issn>
<publisher><publisher-name>Elsevier B.V.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18533276</article-id>
<article-id pub-id-type="pmc">7112888</article-id>
<article-id pub-id-type="publisher-id">S0165-2478(08)00119-3</article-id>
<article-id pub-id-type="doi">10.1016/j.imlet.2008.04.005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV <italic>in vitro</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Xiaohua</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xu</surname>
<given-names>Wei</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tong</surname>
<given-names>Deyan</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ni</surname>
<given-names>Jing</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gao</surname>
<given-names>Haifeng</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Ying</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chu</surname>
<given-names>Yiwei</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Pingping</given-names>
</name>
<xref rid="aff3" ref-type="aff">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Xiaoming</given-names>
</name>
<xref rid="aff3" ref-type="aff">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Xiong</surname>
<given-names>Sidong</given-names>
</name>
<email>sdxiongfd@126.com</email>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="aff2" ref-type="aff">b</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China</aff>
<aff id="aff2"><label>b</label>
Institute of Biomedical Science, Fudan University, PR China</aff>
<aff id="aff3"><label>c</label>
Wuhan Institute of Biological Products, Hubei 430060, PR China</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author at: Institute for ImmunoBiology, Department of Immunology, Shanghai Medical College of Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, PR China. Tel.: +86 21 54237324; fax: +86 21 54237749. <email>sdxiongfd@126.com</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>19</day>
<month>5</month>
<year>2008</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>15</day>
<month>8</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="epub"><day>19</day>
<month>5</month>
<year>2008</year>
</pub-date>
<volume>119</volume>
<issue>1</issue>
<fpage>71</fpage>
<lpage>77</lpage>
<history><date date-type="received"><day>6</day>
<month>2</month>
<year>2008</year>
</date>
<date date-type="rev-recd"><day>11</day>
<month>4</month>
<year>2008</year>
</date>
<date date-type="accepted"><day>22</day>
<month>4</month>
<year>2008</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2008 Elsevier B.V. All rights reserved.</copyright-statement>
<copyright-year>2008</copyright-year>
<copyright-holder>Elsevier B.V.</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract><p>Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S<sub>437–459</sub>
and M<sub>1–20</sub>
. A DNA prime–protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1:160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd>SARS-CoV</kwd>
<kwd>Epitope</kwd>
<kwd>DNA vaccine</kwd>
<kwd>Antibody</kwd>
<kwd>Prime–boost</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Xiaohua" sort="Wang, Xiaohua" uniqKey="Wang X" first="Xiaohua" last="Wang">Xiaohua Wang</name>
</noRegion>
<name sortKey="Chu, Yiwei" sort="Chu, Yiwei" uniqKey="Chu Y" first="Yiwei" last="Chu">Yiwei Chu</name>
<name sortKey="Gao, Haifeng" sort="Gao, Haifeng" uniqKey="Gao H" first="Haifeng" last="Gao">Haifeng Gao</name>
<name sortKey="Li, Pingping" sort="Li, Pingping" uniqKey="Li P" first="Pingping" last="Li">Pingping Li</name>
<name sortKey="Ni, Jing" sort="Ni, Jing" uniqKey="Ni J" first="Jing" last="Ni">Jing Ni</name>
<name sortKey="Tong, Deyan" sort="Tong, Deyan" uniqKey="Tong D" first="Deyan" last="Tong">Deyan Tong</name>
<name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name>
<name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name>
<name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name>
<name sortKey="Xu, Wei" sort="Xu, Wei" uniqKey="Xu W" first="Wei" last="Xu">Wei Xu</name>
<name sortKey="Yang, Xiaoming" sort="Yang, Xiaoming" uniqKey="Yang X" first="Xiaoming" last="Yang">Xiaoming Yang</name>
</country>
</tree>
</affiliations>
</record>
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