Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿
Identifieur interne : 000E16 ( Pmc/Checkpoint ); précédent : 000E15; suivant : 000E17Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿
Auteurs : Baojing Lu ; Ling Tao ; Ting Wang ; Zhenhua Zheng ; Bao Li ; Ze Chen ; Yi Huang ; Qinxue Hu ; Hanzhong WangSource :
- Clinical and Vaccine Immunology : CVI [ 1556-6811 ] ; 2008.
Abstract
Vaccine development for severe acute respiratory syndrome coronavirus (SARS-CoV) has mainly focused on the spike (S) protein. However, the variation of the S gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against SARS-like CoV (SL-CoV). Recently, a more conserved group-specific open reading frame (ORF), the 3a gene, was found in both SARS-CoV and SL-CoV. Here, we studied the immunogenicity of human SARS-CoV 3a and bat SL-CoV 3a DNA vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. Our results showed that high levels of specific humoral responses were induced by SARS-CoV 3a and SL-CoV 3a DNA vaccines. Furthermore, a strong Th1-based cellular immune response was stimulated by both DNA vaccines. The vaccines stimulated gamma interferon production mainly by CD8+ T cells and interleukin-2 (IL-2) mainly by CD4+ T cells. Of interest, the frequency of IL-2-positive cells elicited by the SARS-CoV 3a DNA vaccine was significantly higher than that elicited by the SL-CoV 3a DNA vaccine. In summary, our study provides a reference for designing cross-protective DNA vaccines based on the group-specific ORFs of CoVs.
Url:
DOI: 10.1128/CVI.00261-08
PubMed: 18987164
PubMed Central: 2620671
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:2620671Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice<xref ref-type="fn" rid="fn1">▿</xref> </title><author><name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18987164</idno><idno type="pmc">2620671</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620671</idno><idno type="RBID">PMC:2620671</idno><idno type="doi">10.1128/CVI.00261-08</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000633</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000633</idno><idno type="wicri:Area/Pmc/Curation">000633</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000633</idno><idno type="wicri:Area/Pmc/Checkpoint">000E16</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000E16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice<xref ref-type="fn" rid="fn1">▿</xref> </title><author><name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical and Vaccine Immunology : CVI</title><idno type="ISSN">1556-6811</idno><idno type="eISSN">1556-679X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Vaccine development for severe acute respiratory syndrome coronavirus (SARS-CoV) has mainly focused on the spike (S) protein. However, the variation of the S gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against SARS-like CoV (SL-CoV). Recently, a more conserved group-specific open reading frame (ORF), the 3a gene, was found in both SARS-CoV and SL-CoV. Here, we studied the immunogenicity of human SARS-CoV 3a and bat SL-CoV 3a DNA vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. Our results showed that high levels of specific humoral responses were induced by SARS-CoV 3a and SL-CoV 3a DNA vaccines. Furthermore, a strong Th1-based cellular immune response was stimulated by both DNA vaccines. The vaccines stimulated gamma interferon production mainly by CD8<sup>+</sup> T cells and interleukin-2 (IL-2) mainly by CD4<sup>+</sup> T cells. Of interest, the frequency of IL-2-positive cells elicited by the SARS-CoV 3a DNA vaccine was significantly higher than that elicited by the SL-CoV 3a DNA vaccine. In summary, our study provides a reference for designing cross-protective DNA vaccines based on the group-specific ORFs of CoVs.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Vaccine Immunol</journal-id><journal-id journal-id-type="publisher-id">cdli</journal-id><journal-title>Clinical and Vaccine Immunology : CVI</journal-title><issn pub-type="ppub">1556-6811</issn><issn pub-type="epub">1556-679X</issn><publisher><publisher-name>American Society for Microbiology (ASM)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18987164</article-id><article-id pub-id-type="pmc">2620671</article-id><article-id pub-id-type="publisher-id">0261-08</article-id><article-id pub-id-type="doi">10.1128/CVI.00261-08</article-id><article-categories><subj-group subj-group-type="heading"><subject>Vaccine Research</subject></subj-group></article-categories><title-group><article-title>Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice<xref ref-type="fn" rid="fn1">▿</xref> </article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Baojing</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Tao</surname><given-names>Ling</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Ting</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zheng</surname><given-names>Zhenhua</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Bao</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Ze</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Qinxue</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hanzhong</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, People's Republic of China,<label>1</label> Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China<label>2</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei, People's Republic of China. Phone and fax: 86-27-87199239. E-mail for Hanzhong Wang: <email>wanghz@wh.iov.cn</email>. E-mail for Qinxue Hu: <email>huqinxue@hotmail.com</email></p></fn></author-notes><pub-date pub-type="ppub"><month>1</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>5</day><month>11</month><year>2008</year></pub-date><volume>16</volume><issue>1</issue><fpage>73</fpage><lpage>77</lpage><history><date date-type="received"><day>18</day><month>7</month><year>2008</year></date><date date-type="rev-recd"><day>21</day><month>8</month><year>2008</year></date><date date-type="accepted"><day>25</day><month>10</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2009, American Society for Microbiology</copyright-statement></permissions><self-uri xlink:title="pdf" xlink:href="zcd00109000073.pdf"></self-uri><abstract><p>Vaccine development for severe acute respiratory syndrome coronavirus (SARS-CoV) has mainly focused on the spike (S) protein. However, the variation of the S gene between viruses may affect the efficacy of a vaccine, particularly for cross-protection against SARS-like CoV (SL-CoV). Recently, a more conserved group-specific open reading frame (ORF), the 3a gene, was found in both SARS-CoV and SL-CoV. Here, we studied the immunogenicity of human SARS-CoV 3a and bat SL-CoV 3a DNA vaccines in mice through electroporation immunization followed by enzyme-linked immunosorbent, enzyme-linked immunospot, and flow cytometry assays. Our results showed that high levels of specific humoral responses were induced by SARS-CoV 3a and SL-CoV 3a DNA vaccines. Furthermore, a strong Th1-based cellular immune response was stimulated by both DNA vaccines. The vaccines stimulated gamma interferon production mainly by CD8<sup>+</sup> T cells and interleukin-2 (IL-2) mainly by CD4<sup>+</sup> T cells. Of interest, the frequency of IL-2-positive cells elicited by the SARS-CoV 3a DNA vaccine was significantly higher than that elicited by the SL-CoV 3a DNA vaccine. In summary, our study provides a reference for designing cross-protective DNA vaccines based on the group-specific ORFs of CoVs.</p></abstract></article-meta></front><floats-wrap><fig position="float" id="f1"><label>FIG. 1.</label><caption><p>Amino acid sequence alignment and in vitro expression of 3a proteins. (A) The amino acid sequences of full-length 3a from SARS-CoV and SL-CoV were aligned with ClustalX 1.83 and edited using GenDoc. (B) The full-length 3a gene from SARS-CoV or SL-CoV was cloned into pcDNA3.1(+) to make pcDNA3.1H3 or pcDNA3.1B3, respectively. The expression of 3a protein was analyzed at 48 h posttransfection by Western blotting. Lane 1, pcDNA3.1; lane 2, pcDNA3.1H3; lane 3, pcDNA3.1B3.</p></caption><graphic xlink:href="zcd0010934200001"></graphic></fig><fig position="float" id="f2"><label>FIG. 2.</label><caption><p>3a-specific antibody responses in immunized mice. Sera from each group were collected before each immunization. Ten days after the final boost, the mice were sacrificed and the specific antibody responses were examined by ELISA. SARS-CoV 3a protein (A) and SL-CoV 3a protein (B) were used as a coating antigen. Data shown are the means ± SD of results from two independent experiments using five animals from each group, with each condition tested in triplicate (*, <italic>P</italic> values of <0.05 for comparison with pcDNA3.1B3). OD, optical density; d.p.i., days postimmunization.</p></caption><graphic xlink:href="zcd0010934200002"></graphic></fig><fig position="float" id="f3"><label>FIG. 3.</label><caption><p>Specific IFN-γ and IL-4 production in immunized mice. Mice were sacrificed 10 days after the final boost, and the frequency of IFN-γ (A)- or IL-4 (B)-producing cells at the single-cell level was determined by an ELISPOT assay. Data shown are the means ± SD of results from two independent experiments using three animals from each group, with each condition tested in triplicate (*, <italic>P</italic> values of <0.05 for comparison with control groups).</p></caption><graphic xlink:href="zcd0010934200003"></graphic></fig><fig position="float" id="f4"><label>FIG. 4.</label><caption><p>Frequencies of IFN-γ- and IL-2-positive cells in CD4<sup>+</sup> or CD8<sup>+</sup> splenocytes. Splenocytes were isolated from vaccinated mice 10 days after the final immunization. IFN-γ (A)- and IL-2 (B)-positive cells in CD4<sup>+</sup> or CD8<sup>+</sup> cell populations were analyzed by flow cytometry. Data shown are the means ± SD of results from two independent experiments using three animals from each group, with each condition tested in triplicate (*, <italic>P</italic> values of <0.05 for comparison with pcDNA3.1B3).</p></caption><graphic xlink:href="zcd0010934200004"></graphic></fig></floats-wrap></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><name sortKey="Li, Bao" sort="Li, Bao" uniqKey="Li B" first="Bao" last="Li">Bao Li</name><name sortKey="Lu, Baojing" sort="Lu, Baojing" uniqKey="Lu B" first="Baojing" last="Lu">Baojing Lu</name><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name><name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E16 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000E16 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:2620671
|texte= Humoral and Cellular Immune Responses Induced by 3a DNA Vaccines against Severe Acute Respiratory Syndrome (SARS) or SARS-Like Coronavirus in Mice▿
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:18987164" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |