Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model
Identifieur interne : 000D39 ( Pmc/Checkpoint ); précédent : 000D38; suivant : 000D40Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model
Auteurs : Hui Hu [République populaire de Chine] ; Xianqing Huang [République populaire de Chine] ; Ling Tao [République populaire de Chine] ; Yi Huang [République populaire de Chine] ; Bao-An Cui [République populaire de Chine] ; Hanzhong Wang [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2009.
Abstract
The development of strategies to augment the immunogenicity of DNA vaccines is critical for improving their clinical utility. One such strategy involves using the different immune routes with DNA vaccines. In the present study, the immunogenicity of SARS-CoV nucleocapsid DNA vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated
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DOI: 10.1016/j.vaccine.2009.01.021
PubMed: 19186202
PubMed Central: 7115532
Affiliations:
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populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Xianqing" sort="Huang, Xianqing" uniqKey="Huang X" first="Xianqing" last="Huang">Xianqing Huang</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19186202</idno><idno type="pmc">7115532</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115532</idno><idno type="RBID">PMC:7115532</idno><idno type="doi">10.1016/j.vaccine.2009.01.021</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">001532</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001532</idno><idno type="wicri:Area/Pmc/Curation">001532</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001532</idno><idno type="wicri:Area/Pmc/Checkpoint">000D39</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model</title><author><name sortKey="Hu, Hui" sort="Hu, Hui" uniqKey="Hu H" first="Hui" last="Hu">Hui Hu</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Xianqing" sort="Huang, Xianqing" uniqKey="Huang X" first="Xianqing" last="Huang">Xianqing Huang</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name><affiliation wicri:level="1"><nlm:aff id="aff2">College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002</wicri:regionArea><wicri:noRegion>Henan 450002</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The development of strategies to augment the immunogenicity of DNA vaccines is critical for improving their clinical utility. One such strategy involves using the different immune routes with DNA vaccines. In the present study, the immunogenicity of SARS-CoV nucleocapsid DNA vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated <italic>Salmonella typhimurium</italic>), was compared in mouse model. The comparison between the three vaccination routes indicated that immunization intramuscularly induced a moderate T cell response and antibody response. Mice administrated by electroporation induced the highest antibody response among the three immunization groups and a mid-level of cellular response. In contrast, the orally DNA vaccine evoked vigorous T cell response and a weak antibody production. These results indicated that the distinct types of immune responses were generated by the different routes of DNA immunization. In addition, our results also show that the delivery of DNA vaccines by electroporation and orally using live-attenuated Salmonella <italic>in vivo</italic> is an effective method to increase the immune responses. Further studies could be carried out using a combination strategy of both oral and electroporation immunizations to stimulate higher cellular and humoral immune responses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Stadler, K" uniqKey="Stadler K">K. Stadler</name></author><author><name sortKey="Masignani, V" uniqKey="Masignani V">V. Masignani</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M. Eickmann</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name></author><author><name sortKey="Abrignani, S" uniqKey="Abrignani S">S. Abrignani</name></author><author><name sortKey="Klenk, H D" uniqKey="Klenk H">H.D. Klenk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M. Schutten</name></author><author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G. van Amerongen</name></author><author><name sortKey="Van Doornum, G J" uniqKey="Van Doornum G">G.J. van Doornum</name></author><author><name sortKey="Van Den Hoogen, B G" uniqKey="Van Den Hoogen B">B.G. van den Hoogen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D. Erdman</name></author><author><name sortKey="Goldsmith, C S" uniqKey="Goldsmith C">C.S. Goldsmith</name></author><author><name sortKey="Zaki, S R" uniqKey="Zaki S">S.R. Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T. Peret</name></author><author><name sortKey="Emery, S" uniqKey="Emery S">S. Emery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name></author><author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. Lai</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Yam, L Y" uniqKey="Yam L">L.Y. Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W. Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name></author><author><name sortKey="Jones, S J" uniqKey="Jones S">S.J. Jones</name></author><author><name sortKey="Astell, C R" uniqKey="Astell C">C.R. Astell</name></author><author><name sortKey="Holt, R A" uniqKey="Holt R">R.A. Holt</name></author><author><name sortKey="Brooks Wilson, A" uniqKey="Brooks Wilson A">A. Brooks-Wilson</name></author><author><name sortKey="Butterfield, Y S" uniqKey="Butterfield Y">Y.S. Butterfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name></author><author><name sortKey="Oberste, M S" uniqKey="Oberste M">M.S. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S.S. Monroe</name></author><author><name sortKey="Nix, W A" uniqKey="Nix W">W.A. Nix</name></author><author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R. Campagnoli</name></author><author><name sortKey="Icenogle, J P" uniqKey="Icenogle J">J.P. Icenogle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Christian, M D" uniqKey="Christian M">M.D. Christian</name></author><author><name sortKey="Poutanen, S M" uniqKey="Poutanen S">S.M. Poutanen</name></author><author><name sortKey="Loutfy, M R" uniqKey="Loutfy M">M.R. Loutfy</name></author><author><name sortKey="Muller, M P" uniqKey="Muller M">M.P. Muller</name></author><author><name sortKey="Low, D E" uniqKey="Low D">D.E. Low</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, C" uniqKey="Liu C">C. Liu</name></author><author><name sortKey="Kokuho, T" uniqKey="Kokuho T">T. Kokuho</name></author><author><name sortKey="Kubota, T" uniqKey="Kubota T">T. Kubota</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S. Watanabe</name></author><author><name sortKey="Inumaru, S" uniqKey="Inumaru S">S. Inumaru</name></author><author><name sortKey="Yokomizo, Y" uniqKey="Yokomizo Y">Y. Yokomizo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K. Narayanan</name></author><author><name sortKey="Chen, C J" uniqKey="Chen C">C.J. Chen</name></author><author><name sortKey="Maeda, J" uniqKey="Maeda J">J. Maeda</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L. Kuo</name></author><author><name sortKey="Masters, P S" uniqKey="Masters P">P.S. Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parker, M M" uniqKey="Parker M">M.M. Parker</name></author><author><name sortKey="Masters, P S" uniqKey="Masters P">P.S. Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hohdatsu, T" uniqKey="Hohdatsu T">T. Hohdatsu</name></author><author><name sortKey="Yamato, H" uniqKey="Yamato H">H. Yamato</name></author><author><name sortKey="Ohkawa, T" uniqKey="Ohkawa T">T. Ohkawa</name></author><author><name sortKey="Kaneko, M" uniqKey="Kaneko M">M. Kaneko</name></author><author><name sortKey="Motokawa, K" uniqKey="Motokawa K">K. Motokawa</name></author><author><name sortKey="Kusuhara, H" uniqKey="Kusuhara H">H. Kusuhara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anton, I M" uniqKey="Anton I">I.M. Anton</name></author><author><name sortKey="Gonzalez, S" uniqKey="Gonzalez S">S. Gonzalez</name></author><author><name sortKey="Bullido, M J" uniqKey="Bullido M">M.J. Bullido</name></author><author><name sortKey="Corsin, M" uniqKey="Corsin M">M. Corsin</name></author><author><name sortKey="Risco, C" uniqKey="Risco C">C. Risco</name></author><author><name sortKey="Langeveld, J P" uniqKey="Langeveld J">J.P. Langeveld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, M S" uniqKey="Chang M">M.S. Chang</name></author><author><name sortKey="Lu, Y T" uniqKey="Lu Y">Y.T. Lu</name></author><author><name sortKey="Ho, S T" uniqKey="Ho S">S.T. Ho</name></author><author><name sortKey="Wu, C C" uniqKey="Wu C">C.C. Wu</name></author><author><name sortKey="Wei, T Y" uniqKey="Wei T">T.Y. Wei</name></author><author><name sortKey="Chen, C J" uniqKey="Chen C">C.J. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Pei, D" uniqKey="Pei D">D. Pei</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Song, Y" uniqKey="Song Y">Y. Song</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, L R" uniqKey="Huang L">L.R. Huang</name></author><author><name sortKey="Chiu, C M" uniqKey="Chiu C">C.M. Chiu</name></author><author><name sortKey="Yeh, S H" uniqKey="Yeh S">S.H. Yeh</name></author><author><name sortKey="Huang, W H" uniqKey="Huang W">W.H. Huang</name></author><author><name sortKey="Hsueh, P R" uniqKey="Hsueh P">P.R. Hsueh</name></author><author><name sortKey="Yang, W Z" uniqKey="Yang W">W.Z. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, M S" uniqKey="Zhu M">M.S. Zhu</name></author><author><name sortKey="Pan, Y" uniqKey="Pan Y">Y. Pan</name></author><author><name sortKey="Chen, H Q" uniqKey="Chen H">H.Q. Chen</name></author><author><name sortKey="Shen, Y" uniqKey="Shen Y">Y. Shen</name></author><author><name sortKey="Wang, X C" uniqKey="Wang X">X.C. Wang</name></author><author><name sortKey="Sun, Y J" uniqKey="Sun Y">Y.J. Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Y J" uniqKey="Tan Y">Y.J. Tan</name></author><author><name sortKey="Goh, P Y" uniqKey="Goh P">P.Y. Goh</name></author><author><name sortKey="Fielding, B C" uniqKey="Fielding B">B.C. Fielding</name></author><author><name sortKey="Shen, S" uniqKey="Shen S">S. Shen</name></author><author><name sortKey="Chou, C F" uniqKey="Chou C">C.F. Chou</name></author><author><name sortKey="Fu, J L" uniqKey="Fu J">J.L. Fu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Timani, K A" uniqKey="Timani K">K.A. Timani</name></author><author><name sortKey="Ye, L" uniqKey="Ye L">L. Ye</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y. Zhu</name></author><author><name sortKey="Wu, Z" uniqKey="Wu Z">Z. Wu</name></author><author><name sortKey="Gong, Z" uniqKey="Gong Z">Z. Gong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author><author><name sortKey="Yuan, Z" uniqKey="Yuan Z">Z. Yuan</name></author><author><name sortKey="Matsumoto, M" uniqKey="Matsumoto M">M. Matsumoto</name></author><author><name sortKey="Hengge, U R" uniqKey="Hengge U">U.R. Hengge</name></author><author><name sortKey="Chang, Y F" uniqKey="Chang Y">Y.F. Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, W" uniqKey="Gao W">W. Gao</name></author><author><name sortKey="Tamin, A" uniqKey="Tamin A">A. Tamin</name></author><author><name sortKey="Soloff, A" uniqKey="Soloff A">A. Soloff</name></author><author><name sortKey="D Iuto, L" uniqKey="D Iuto L">L. D’Aiuto</name></author><author><name sortKey="Nwanegbo, E" uniqKey="Nwanegbo E">E. Nwanegbo</name></author><author><name sortKey="Robbins, P D" uniqKey="Robbins P">P.D. Robbins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, T W" uniqKey="Kim T">T.W. Kim</name></author><author><name sortKey="Lee, J H" uniqKey="Lee J">J.H. Lee</name></author><author><name sortKey="Hung, C F" uniqKey="Hung C">C.F. Hung</name></author><author><name sortKey="Peng, S" uniqKey="Peng S">S. Peng</name></author><author><name sortKey="Roden, R" uniqKey="Roden R">R. Roden</name></author><author><name sortKey="Wang, M C" uniqKey="Wang M">M.C. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jin, H" uniqKey="Jin H">H. Jin</name></author><author><name sortKey="Xiao, C" uniqKey="Xiao C">C. Xiao</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Kang, Y" uniqKey="Kang Y">Y. Kang</name></author><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y. Ma</name></author><author><name sortKey="Zhu, K" uniqKey="Zhu K">K. Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okada, M" uniqKey="Okada M">M. Okada</name></author><author><name sortKey="Takemoto, Y" uniqKey="Takemoto Y">Y. Takemoto</name></author><author><name sortKey="Okuno, Y" uniqKey="Okuno Y">Y. Okuno</name></author><author><name sortKey="Hashimoto, S" uniqKey="Hashimoto S">S. Hashimoto</name></author><author><name sortKey="Yoshida, S" uniqKey="Yoshida S">S. Yoshida</name></author><author><name sortKey="Fukunaga, Y" uniqKey="Fukunaga Y">Y. Fukunaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gilbert, S C" uniqKey="Gilbert S">S.C. Gilbert</name></author><author><name sortKey="Moorthy, V S" uniqKey="Moorthy V">V.S. Moorthy</name></author><author><name sortKey="Andrews, L" uniqKey="Andrews L">L. Andrews</name></author><author><name sortKey="Pathan, A A" uniqKey="Pathan A">A.A. Pathan</name></author><author><name sortKey="Mcconkey, S J" uniqKey="Mcconkey S">S.J. McConkey</name></author><author><name sortKey="Vuola, J M" uniqKey="Vuola J">J.M. Vuola</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Girard, M P" uniqKey="Girard M">M.P. Girard</name></author><author><name sortKey="Osmanov, S K" uniqKey="Osmanov S">S.K. Osmanov</name></author><author><name sortKey="Kieny, M P" uniqKey="Kieny M">M.P. Kieny</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leblanc, R" uniqKey="Leblanc R">R. LeBlanc</name></author><author><name sortKey="Vasquez, Y" uniqKey="Vasquez Y">Y. Vasquez</name></author><author><name sortKey="Hannaman, D" uniqKey="Hannaman D">D. Hannaman</name></author><author><name sortKey="Kumar, N" uniqKey="Kumar N">N. Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scheerlinck, J P" uniqKey="Scheerlinck J">J.P. Scheerlinck</name></author><author><name sortKey="Karlis, J" uniqKey="Karlis J">J. Karlis</name></author><author><name sortKey="Tjelle, T E" uniqKey="Tjelle T">T.E. Tjelle</name></author><author><name sortKey="Presidente, P J" uniqKey="Presidente P">P.J. Presidente</name></author><author><name sortKey="Mathiesen, I" uniqKey="Mathiesen I">I. Mathiesen</name></author><author><name sortKey="Newton, S E" uniqKey="Newton S">S.E. Newton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dougan, G" uniqKey="Dougan G">G. Dougan</name></author><author><name sortKey="Hormaeche, C E" uniqKey="Hormaeche C">C.E. Hormaeche</name></author><author><name sortKey="Maskell, D J" uniqKey="Maskell D">D.J. Maskell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ashby, D" uniqKey="Ashby D">D. Ashby</name></author><author><name sortKey="Leduc, I" uniqKey="Leduc I">I. Leduc</name></author><author><name sortKey="Lauzon, W" uniqKey="Lauzon W">W. Lauzon</name></author><author><name sortKey="Lee, B C" uniqKey="Lee B">B.C. Lee</name></author><author><name sortKey="Singhal, N" uniqKey="Singhal N">N. Singhal</name></author><author><name sortKey="Cameron, D W" uniqKey="Cameron D">D.W. Cameron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benyacoub, J" uniqKey="Benyacoub J">J. Benyacoub</name></author><author><name sortKey="Hopkins, S" uniqKey="Hopkins S">S. Hopkins</name></author><author><name sortKey="Potts, A" uniqKey="Potts A">A. Potts</name></author><author><name sortKey="Kelly, S" uniqKey="Kelly S">S. Kelly</name></author><author><name sortKey="Kraehenbuhl, J P" uniqKey="Kraehenbuhl J">J.P. Kraehenbuhl</name></author><author><name sortKey="Curtiss, R" uniqKey="Curtiss R">R. Curtiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Flo, J" uniqKey="Flo J">J. Flo</name></author><author><name sortKey="Tisminetzky, S" uniqKey="Tisminetzky S">S. Tisminetzky</name></author><author><name sortKey="Baralle, F" uniqKey="Baralle F">F. Baralle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Darji, A" uniqKey="Darji A">A. Darji</name></author><author><name sortKey="Zur Lage, S" uniqKey="Zur Lage S">S. zur Lage</name></author><author><name sortKey="Garbe, A I" uniqKey="Garbe A">A.I. Garbe</name></author><author><name sortKey="Chakraborty, T" uniqKey="Chakraborty T">T. Chakraborty</name></author><author><name sortKey="Weiss, S" uniqKey="Weiss S">S. Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harrison, J A" uniqKey="Harrison J">J.A. Harrison</name></author><author><name sortKey="Villarreal Ramos, B" uniqKey="Villarreal Ramos B">B. Villarreal-Ramos</name></author><author><name sortKey="Mastroeni, P" uniqKey="Mastroeni P">P. Mastroeni</name></author><author><name sortKey="Demarco De Hormaeche, R" uniqKey="Demarco De Hormaeche R">R. Demarco de Hormaeche</name></author><author><name sortKey="Hormaeche, C E" uniqKey="Hormaeche C">C.E. Hormaeche</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Froebel, K S" uniqKey="Froebel K">K.S. Froebel</name></author><author><name sortKey="Pakker, N G" uniqKey="Pakker N">N.G. Pakker</name></author><author><name sortKey="Aiuti, F" uniqKey="Aiuti F">F. Aiuti</name></author><author><name sortKey="Bofill, M" uniqKey="Bofill M">M. Bofill</name></author><author><name sortKey="Choremi Papadopoulou, H" uniqKey="Choremi Papadopoulou H">H. Choremi-Papadopoulou</name></author><author><name sortKey="Economidou, J" uniqKey="Economidou J">J. Economidou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Rose, R" uniqKey="De Rose R">R. De Rose</name></author><author><name sortKey="Tennent, J" uniqKey="Tennent J">J. Tennent</name></author><author><name sortKey="Mcwaters, P" uniqKey="Mcwaters P">P. McWaters</name></author><author><name sortKey="Chaplin, P J" uniqKey="Chaplin P">P.J. Chaplin</name></author><author><name sortKey="Wood, P R" uniqKey="Wood P">P.R. Wood</name></author><author><name sortKey="Kimpton, W" uniqKey="Kimpton W">W. Kimpton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, H" uniqKey="Hu H">H. Hu</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Tao, L" uniqKey="Tao L">L. Tao</name></author><author><name sortKey="Bai, B" uniqKey="Bai B">B. Bai</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z. Zhang</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otten, G R" uniqKey="Otten G">G.R. Otten</name></author><author><name sortKey="Schaefer, M" uniqKey="Schaefer M">M. Schaefer</name></author><author><name sortKey="Doe, B" uniqKey="Doe B">B. Doe</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Megede, J Z" uniqKey="Megede J">J.Z. Megede</name></author><author><name sortKey="Donnelly, J" uniqKey="Donnelly J">J. Donnelly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widera, G" uniqKey="Widera G">G. Widera</name></author><author><name sortKey="Austin, M" uniqKey="Austin M">M. Austin</name></author><author><name sortKey="Rabussay, D" uniqKey="Rabussay D">D. Rabussay</name></author><author><name sortKey="Goldbeck, C" uniqKey="Goldbeck C">C. Goldbeck</name></author><author><name sortKey="Barnett, S W" uniqKey="Barnett S">S.W. Barnett</name></author><author><name sortKey="Chen, M" uniqKey="Chen M">M. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otten, G" uniqKey="Otten G">G. Otten</name></author><author><name sortKey="Schaefer, M" uniqKey="Schaefer M">M. Schaefer</name></author><author><name sortKey="Doe, B" uniqKey="Doe B">B. Doe</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Srivastava, I" uniqKey="Srivastava I">I. Srivastava</name></author><author><name sortKey="Zur Megede, J" uniqKey="Zur Megede J">J. zur Megede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiang, R" uniqKey="Xiang R">R. Xiang</name></author><author><name sortKey="Lode, H N" uniqKey="Lode H">H.N. Lode</name></author><author><name sortKey="Chao, T H" uniqKey="Chao T">T.H. Chao</name></author><author><name sortKey="Ruehlmann, J M" uniqKey="Ruehlmann J">J.M. Ruehlmann</name></author><author><name sortKey="Dolman, C S" uniqKey="Dolman C">C.S. Dolman</name></author><author><name sortKey="Rodriguez, F" uniqKey="Rodriguez F">F. Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Wong, L P" uniqKey="Wong L">L.P. Wong</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J. Zheng</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shata, M T" uniqKey="Shata M">M.T. Shata</name></author><author><name sortKey="Reitz, M S" uniqKey="Reitz M">M.S. Reitz</name></author><author><name sortKey="Devico, A L" uniqKey="Devico A">A.L. DeVico</name></author><author><name sortKey="Lewis, G K" uniqKey="Lewis G">G.K. Lewis</name></author><author><name sortKey="Hone, D M" uniqKey="Hone D">D.M. Hone</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id><journal-id journal-id-type="iso-abbrev">Vaccine</journal-id><journal-title-group><journal-title>Vaccine</journal-title></journal-title-group><issn pub-type="ppub">0264-410X</issn><issn pub-type="epub">1873-2518</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19186202</article-id><article-id pub-id-type="pmc">7115532</article-id><article-id pub-id-type="publisher-id">S0264-410X(09)00059-0</article-id><article-id pub-id-type="doi">10.1016/j.vaccine.2009.01.021</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Comparative analysis of the immunogenicity of SARS-CoV nucleocapsid DNA vaccine administrated with different routes in mouse model</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Hui</given-names></name><email>wanghz@wh.iov.cn</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Xianqing</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Tao</surname><given-names>Ling</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Yi</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Cui</surname><given-names>Bao-an</given-names></name><xref rid="aff2" ref-type="aff">b</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hanzhong</given-names></name><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor2" ref-type="corresp">⁎⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China</aff><aff id="aff2"><label>b</label>College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author at: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, PR China. Tel.: +86 27 87199239; fax: +86 27 87198072.</corresp><corresp id="cor2"><label>⁎⁎</label>Corresponding author. Tel.: +86 27 87197295; fax: +86 27 87198072.</corresp></author-notes><pub-date pub-type="pmc-release"><day>30</day><month>1</month><year>2009</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>10</day><month>3</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>30</day><month>1</month><year>2009</year></pub-date><volume>27</volume><issue>11</issue><fpage>1758</fpage><lpage>1763</lpage><history><date date-type="received"><day>15</day><month>12</month><year>2008</year></date><date date-type="rev-recd"><day>3</day><month>1</month><year>2009</year></date><date date-type="accepted"><day>11</day><month>1</month><year>2009</year></date></history><permissions><copyright-statement>Copyright © 2009 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2009</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>The development of strategies to augment the immunogenicity of DNA vaccines is critical for improving their clinical utility. One such strategy involves using the different immune routes with DNA vaccines. In the present study, the immunogenicity of SARS-CoV nucleocapsid DNA vaccine, induced by using the current routine vaccination routes (intramuscularly, by electroporation, or orally using live-attenuated <italic>Salmonella typhimurium</italic>), was compared in mouse model. The comparison between the three vaccination routes indicated that immunization intramuscularly induced a moderate T cell response and antibody response. Mice administrated by electroporation induced the highest antibody response among the three immunization groups and a mid-level of cellular response. In contrast, the orally DNA vaccine evoked vigorous T cell response and a weak antibody production. These results indicated that the distinct types of immune responses were generated by the different routes of DNA immunization. In addition, our results also show that the delivery of DNA vaccines by electroporation and orally using live-attenuated Salmonella <italic>in vivo</italic> is an effective method to increase the immune responses. Further studies could be carried out using a combination strategy of both oral and electroporation immunizations to stimulate higher cellular and humoral immune responses.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Nucleocapsid protein</kwd><kwd>DNA vaccine</kwd><kwd>Immunization routes</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Hu, Hui" sort="Hu, Hui" uniqKey="Hu H" first="Hui" last="Hu">Hui Hu</name></region><name sortKey="Cui, Bao An" sort="Cui, Bao An" uniqKey="Cui B" first="Bao-An" last="Cui">Bao-An Cui</name><name sortKey="Hu, Hui" sort="Hu, Hui" uniqKey="Hu H" first="Hui" last="Hu">Hui Hu</name><name sortKey="Huang, Xianqing" sort="Huang, Xianqing" uniqKey="Huang X" first="Xianqing" last="Huang">Xianqing Huang</name><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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