Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV
Identifieur interne : 000B63 ( Pmc/Checkpoint ); précédent : 000B62; suivant : 000B64Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV
Auteurs : Ye V. Liu [États-Unis] ; Michael J. Massare [États-Unis] ; Dale L. Barnard [États-Unis] ; Thomas Kort [États-Unis] ; Margret Nathan [États-Unis] ; Lei Wang [États-Unis] ; Gale Smith [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2011.
Abstract
SARS-CoV was the cause of the global pandemic in 2003 that infected over 8000 people in 8 months. Vaccines against SARS are still not available. We developed a novel method to produce high levels of a recombinant SARS virus-like particles (VLPs) vaccine containing the SARS spike (S) protein and the influenza M1 protein using the baculovirus insect cell expression system. These chimeric SARS VLPs have a similar size and morphology to the wild type SARS-CoV. We tested the immunogenicity and protective efficacy of purified chimeric SARS VLPs and full length SARS S protein vaccines in a mouse lethal challenge model. The SARS VLP vaccine, containing 0.8 μg of SARS S protein, completely protected mice from death when administered intramuscular (IM) or intranasal (IN) routes in the absence of an adjuvant. Likewise, the SARS VLP vaccine, containing 4 μg of S protein without adjuvant, reduced lung virus titer to below detectable level, protected mice from weight loss, and elicited a high level of neutralizing antibodies against SARS-CoV. Sf9 cell-produced full length purified SARS S protein was also an effective vaccine against SARS-CoV but only when co-administered IM with aluminum hydroxide. SARS-CoV VLPs are highly immunogenic and induce neutralizing antibodies and provide protection against lethal challenge. Sf9 cell-based VLP vaccines are a potential tool to provide protection against novel pandemic agents.
Url:
DOI: 10.1016/j.vaccine.2011.06.111
PubMed: 21762752
PubMed Central: 3165014
Affiliations:
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Liu</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Massare, Michael J" sort="Massare, Michael J" uniqKey="Massare M" first="Michael J." last="Massare">Michael J. Massare</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Barnard, Dale L" sort="Barnard, Dale L" uniqKey="Barnard D" first="Dale L." last="Barnard">Dale L. Barnard</name><affiliation wicri:level="2"><nlm:aff id="aff0010">Institute for Antiviral Research, Utah State University, Logan, UT 84322, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute for Antiviral Research, Utah State University, Logan, UT 84322</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Kort, Thomas" sort="Kort, Thomas" uniqKey="Kort T" first="Thomas" last="Kort">Thomas Kort</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Nathan, Margret" sort="Nathan, Margret" uniqKey="Nathan M" first="Margret" last="Nathan">Margret Nathan</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Smith, Gale" sort="Smith, Gale" uniqKey="Smith G" first="Gale" last="Smith">Gale Smith</name><affiliation wicri:level="2"><nlm:aff id="aff0005">Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>SARS-CoV was the cause of the global pandemic in 2003 that infected over 8000 people in 8 months. Vaccines against SARS are still not available. We developed a novel method to produce high levels of a recombinant SARS virus-like particles (VLPs) vaccine containing the SARS spike (S) protein and the influenza M1 protein using the baculovirus insect cell expression system. These chimeric SARS VLPs have a similar size and morphology to the wild type SARS-CoV. We tested the immunogenicity and protective efficacy of purified chimeric SARS VLPs and full length SARS S protein vaccines in a mouse lethal challenge model. The SARS VLP vaccine, containing 0.8 μg of SARS S protein, completely protected mice from death when administered intramuscular (IM) or intranasal (IN) routes in the absence of an adjuvant. Likewise, the SARS VLP vaccine, containing 4 μg of S protein without adjuvant, reduced lung virus titer to below detectable level, protected mice from weight loss, and elicited a high level of neutralizing antibodies against SARS-CoV. Sf9 cell-produced full length purified SARS S protein was also an effective vaccine against SARS-CoV but only when co-administered IM with aluminum hydroxide. SARS-CoV VLPs are highly immunogenic and induce neutralizing antibodies and provide protection against lethal challenge. Sf9 cell-based VLP vaccines are a potential tool to provide protection against novel pandemic agents.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author><author><name sortKey="Dediego, M L" uniqKey="Dediego M">M.L. Dediego</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E. Alvarez</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D. Deming</name></author><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T. Sheahan</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id><journal-id journal-id-type="iso-abbrev">Vaccine</journal-id><journal-title-group><journal-title>Vaccine</journal-title></journal-title-group><issn pub-type="ppub">0264-410X</issn><issn pub-type="epub">1873-2518</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21762752</article-id><article-id pub-id-type="pmc">3165014</article-id><article-id pub-id-type="publisher-id">S0264-410X(11)01005-X</article-id><article-id pub-id-type="doi">10.1016/j.vaccine.2011.06.111</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Ye V.</given-names></name><email>yliu@novavax.com</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Massare</surname><given-names>Michael J.</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Barnard</surname><given-names>Dale L.</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Kort</surname><given-names>Thomas</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Nathan</surname><given-names>Margret</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Lei</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Smith</surname><given-names>Gale</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Novavax Inc., 9920 Belward Campus Drive, Rockville, MD 20850, United States</aff><aff id="aff0010"><label>b</label>Institute for Antiviral Research, Utah State University, Logan, UT 84322, United States</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author. Tel.: +1 240 268 2069; fax: +1 240 268 2100. <email>yliu@novavax.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>14</day><month>7</month><year>2011</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>2</day><month>9</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>14</day><month>7</month><year>2011</year></pub-date><volume>29</volume><issue>38</issue><fpage>6606</fpage><lpage>6613</lpage><history><date date-type="received"><day>16</day><month>2</month><year>2011</year></date><date date-type="rev-recd"><day>22</day><month>3</month><year>2011</year></date><date date-type="accepted"><day>29</day><month>6</month><year>2011</year></date></history><permissions><copyright-statement>Copyright © 2011 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2011</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>SARS-CoV was the cause of the global pandemic in 2003 that infected over 8000 people in 8 months. Vaccines against SARS are still not available. We developed a novel method to produce high levels of a recombinant SARS virus-like particles (VLPs) vaccine containing the SARS spike (S) protein and the influenza M1 protein using the baculovirus insect cell expression system. These chimeric SARS VLPs have a similar size and morphology to the wild type SARS-CoV. We tested the immunogenicity and protective efficacy of purified chimeric SARS VLPs and full length SARS S protein vaccines in a mouse lethal challenge model. The SARS VLP vaccine, containing 0.8 μg of SARS S protein, completely protected mice from death when administered intramuscular (IM) or intranasal (IN) routes in the absence of an adjuvant. Likewise, the SARS VLP vaccine, containing 4 μg of S protein without adjuvant, reduced lung virus titer to below detectable level, protected mice from weight loss, and elicited a high level of neutralizing antibodies against SARS-CoV. Sf9 cell-produced full length purified SARS S protein was also an effective vaccine against SARS-CoV but only when co-administered IM with aluminum hydroxide. SARS-CoV VLPs are highly immunogenic and induce neutralizing antibodies and provide protection against lethal challenge. Sf9 cell-based VLP vaccines are a potential tool to provide protection against novel pandemic agents.</p></abstract><kwd-group><title>Keywords</title><kwd>Severe acute respiratory syndrome</kwd><kwd>Virus like particles</kwd><kwd>Lung virus titer</kwd><kwd>Neutralizing antibody</kwd><kwd>Baculovirus</kwd><kwd>Influenza</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Utah</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Liu, Ye V" sort="Liu, Ye V" uniqKey="Liu Y" first="Ye V." last="Liu">Ye V. Liu</name></region><name sortKey="Barnard, Dale L" sort="Barnard, Dale L" uniqKey="Barnard D" first="Dale L." last="Barnard">Dale L. Barnard</name><name sortKey="Kort, Thomas" sort="Kort, Thomas" uniqKey="Kort T" first="Thomas" last="Kort">Thomas Kort</name><name sortKey="Massare, Michael J" sort="Massare, Michael J" uniqKey="Massare M" first="Michael J." last="Massare">Michael J. Massare</name><name sortKey="Nathan, Margret" sort="Nathan, Margret" uniqKey="Nathan M" first="Margret" last="Nathan">Margret Nathan</name><name sortKey="Smith, Gale" sort="Smith, Gale" uniqKey="Smith G" first="Gale" last="Smith">Gale Smith</name><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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