Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives
Identifieur interne : 000A38 ( Pmc/Checkpoint ); précédent : 000A37; suivant : 000A39Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives
Auteurs : Manmohan Parida ; Jyoti Shukla ; Shashi Sharma ; P. V. Lakshmana RaoSource :
- Proceedings of the National Academy of Sciences, India. Section B [ 0369-8211 ] ; 2012.
Abstract
The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. The real-time assays viz; SYBR green I based real time RT-PCR and RT-LAMP have been developed for rapid detection as well as typing of some of the emerging arboviruses of biomedical importance viz; Dengue, Japanese Encephalitis, Chikungunya, West Nile, SARS and Swine Flu etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity and specificity. One of the most important advantages of RT-LAMP is its field applicability, without requirement of any sophisticated equipments. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.
Url:
DOI: 10.1007/s40011-011-0015-8
PubMed: 32226203
PubMed Central: 7099323
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V." last="Lakshmana Rao">P. V. Lakshmana Rao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences, India. Section B</title><idno type="ISSN">0369-8211</idno><idno type="eISSN">2250-1746</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. The real-time assays viz; SYBR green I based real time RT-PCR and RT-LAMP have been developed for rapid detection as well as typing of some of the emerging arboviruses of biomedical importance viz; Dengue, Japanese Encephalitis, Chikungunya, West Nile, SARS and Swine Flu etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity and specificity. One of the most important advantages of RT-LAMP is its field applicability, without requirement of any sophisticated equipments. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Espy, Mj" uniqKey="Espy M">MJ Espy</name></author><author><name sortKey="Uhl, Jr" uniqKey="Uhl J">JR Uhl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Ab" uniqKey="Chan A">AB Chan</name></author><author><name sortKey="Fox, Jd" uniqKey="Fox J">JD Fox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parida, Mm" uniqKey="Parida M">MM Parida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ratcliff, Rm" uniqKey="Ratcliff R">RM Ratcliff</name></author><author><name sortKey="Chang, G" uniqKey="Chang G">G Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Notomi, T" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci India Sect B Biol Sci</journal-id><journal-id journal-id-type="iso-abbrev">Proc Natl Acad Sci India Sect B Biol Sci</journal-id><journal-title-group><journal-title>Proceedings of the National Academy of Sciences, India. Section B</journal-title></journal-title-group><issn pub-type="ppub">0369-8211</issn><issn pub-type="epub">2250-1746</issn><publisher><publisher-name>Springer-Verlag</publisher-name><publisher-loc>India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32226203</article-id><article-id pub-id-type="pmc">7099323</article-id><article-id pub-id-type="publisher-id">15</article-id><article-id pub-id-type="doi">10.1007/s40011-011-0015-8</article-id><article-categories><subj-group subj-group-type="heading"><subject>Review</subject></subj-group></article-categories><title-group><article-title>Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Parida</surname><given-names>Manmohan</given-names></name><address><phone>+91-751-2233495</phone><fax>+91-751-2351148</fax><email>paridamm@rediffmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Shukla</surname><given-names>Jyoti</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Sharma</surname><given-names>Shashi</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Lakshmana Rao</surname><given-names>P. V.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.418940.0</institution-id><institution-id institution-id-type="ISNI">0000000418032027</institution-id><institution>Division of Virology,</institution><institution>Defence Research and Development Establishment (DRDE),</institution></institution-wrap>Jhansi Road, Gwalior, 474002 MP India</aff></contrib-group><pub-date pub-type="epub"><day>18</day><month>1</month><year>2012</year></pub-date><pub-date pub-type="ppub"><year>2012</year></pub-date><volume>82</volume><issue>1</issue><fpage>199</fpage><lpage>207</lpage><history><date date-type="received"><day>16</day><month>9</month><year>2011</year></date><date date-type="accepted"><day>14</day><month>11</month><year>2011</year></date></history><permissions><copyright-statement>© The National Academy of Sciences, India 2012</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. The real-time assays viz; SYBR green I based real time RT-PCR and RT-LAMP have been developed for rapid detection as well as typing of some of the emerging arboviruses of biomedical importance viz; Dengue, Japanese Encephalitis, Chikungunya, West Nile, SARS and Swine Flu etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity and specificity. One of the most important advantages of RT-LAMP is its field applicability, without requirement of any sophisticated equipments. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Real-time RT-PCR</kwd><kwd>RT-LAMP</kwd><kwd>Virus diagnosis</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The National Academy of Sciences, India 2012</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Lakshmana Rao, P V" sort="Lakshmana Rao, P V" uniqKey="Lakshmana Rao P" first="P. V." last="Lakshmana Rao">P. V. Lakshmana Rao</name><name sortKey="Parida, Manmohan" sort="Parida, Manmohan" uniqKey="Parida M" first="Manmohan" last="Parida">Manmohan Parida</name><name sortKey="Sharma, Shashi" sort="Sharma, Shashi" uniqKey="Sharma S" first="Shashi" last="Sharma">Shashi Sharma</name><name sortKey="Shukla, Jyoti" sort="Shukla, Jyoti" uniqKey="Shukla J" first="Jyoti" last="Shukla">Jyoti Shukla</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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