Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin
Identifieur interne : 000973 ( Pmc/Checkpoint ); précédent : 000972; suivant : 000974Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin
Auteurs : Darryl Falzarano ; Emmie De Wit ; Cynthia Martellaro ; Julie Callison ; Vincent J. Munster [États-Unis] ; Heinz FeldmannSource :
- Scientific Reports [ 2045-2322 ] ; 2013.
Abstract
The identification of a novel β coronavirus, nCoV, as the causative agent of severe respiratory illness in humans originating in Saudi Arabia, Qatar and Jordan has raised concerns about the possibility of a coronavirus pandemic similar to that of SARS-CoV. As a definitive treatment regimen has never been thoroughly evaluated for coronavirus infections, there is an urgent need to rapidly identify potential therapeutics to address future cases of nCoV. To determine an intervention strategy, the effect of interferon-α2b and ribavirin on nCoV isolate hCoV-EMC/2012 replication in Vero and LLC-MK2 cells was evaluated. hCoV-EMC/2012 was sensitive to both interferon-α2b and ribavirin alone in Vero and LLC-MK2 cells, but only at relatively high concentrations; however, when combined, lower concentrations of interferon-α2b and ribavirin achieved comparable endpoints. Thus, a combination of interferon-α2b and ribavirin, which are already commonly used in the clinic, may be useful for patient management in the event of future nCoV infections.
Url:
DOI: 10.1038/srep01686
PubMed: 23594967
PubMed Central: 3629412
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:3629412Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin</title>
<author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Martellaro, Cynthia" sort="Martellaro, Cynthia" uniqKey="Martellaro C" first="Cynthia" last="Martellaro">Cynthia Martellaro</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Callison, Julie" sort="Callison, Julie" uniqKey="Callison J" first="Julie" last="Callison">Julie Callison</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name>
<affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id>
<institution>Virus Ecology Unit, Division of Intramural Research,</institution>
<institution>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories,</institution>
</institution-wrap>
Hamilton, MT USA</nlm:aff>
<country>États-Unis</country>
<placeName><region type="state">Montana</region>
</placeName>
<wicri:cityArea>Hamilton</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.21613.37</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 1936 9609</institution-id>
<institution>Department of Medical Microbiology,</institution>
<institution>University of Manitoba,</institution>
</institution-wrap>
Winnipeg, MB Canada</nlm:aff>
<wicri:noCountry code="subfield">MB Canada</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23594967</idno>
<idno type="pmc">3629412</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3629412</idno>
<idno type="RBID">PMC:3629412</idno>
<idno type="doi">10.1038/srep01686</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000469</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000469</idno>
<idno type="wicri:Area/Pmc/Curation">000469</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000469</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000973</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000973</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin</title>
<author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Martellaro, Cynthia" sort="Martellaro, Cynthia" uniqKey="Martellaro C" first="Cynthia" last="Martellaro">Cynthia Martellaro</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Callison, Julie" sort="Callison, Julie" uniqKey="Callison J" first="Julie" last="Callison">Julie Callison</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name>
<affiliation wicri:level="2"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id>
<institution>Virus Ecology Unit, Division of Intramural Research,</institution>
<institution>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories,</institution>
</institution-wrap>
Hamilton, MT USA</nlm:aff>
<country>États-Unis</country>
<placeName><region type="state">Montana</region>
</placeName>
<wicri:cityArea>Hamilton</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation><nlm:aff id="Aff1">Disease Modeling and Transmission Unit, ,</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.21613.37</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 1936 9609</institution-id>
<institution>Department of Medical Microbiology,</institution>
<institution>University of Manitoba,</institution>
</institution-wrap>
Winnipeg, MB Canada</nlm:aff>
<wicri:noCountry code="subfield">MB Canada</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The identification of a novel β coronavirus, nCoV, as the causative agent of severe respiratory illness in humans originating in Saudi Arabia, Qatar and Jordan has raised concerns about the possibility of a coronavirus pandemic similar to that of SARS-CoV. As a definitive treatment regimen has never been thoroughly evaluated for coronavirus infections, there is an urgent need to rapidly identify potential therapeutics to address future cases of nCoV. To determine an intervention strategy, the effect of interferon-α2b and ribavirin on nCoV isolate hCoV-EMC/2012 replication in Vero and LLC-MK2 cells was evaluated. hCoV-EMC/2012 was sensitive to both interferon-α2b and ribavirin alone in Vero and LLC-MK2 cells, but only at relatively high concentrations; however, when combined, lower concentrations of interferon-α2b and ribavirin achieved comparable endpoints. Thus, a combination of interferon-α2b and ribavirin, which are already commonly used in the clinic, may be useful for patient management in the event of future nCoV infections.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name>
</author>
<author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name>
</author>
<author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buchholz, U" uniqKey="Buchholz U">U Buchholz</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wise, J" uniqKey="Wise J">J Wise</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name>
</author>
<author><name sortKey="Dominguez, Sr" uniqKey="Dominguez S">SR Dominguez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sung, Jj" uniqKey="Sung J">JJ Sung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Booth, Cm" uniqKey="Booth C">CM Booth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chan, Ks" uniqKey="Chan K">KS Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chu, Cm" uniqKey="Chu C">CM Chu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Loutfy, Mr" uniqKey="Loutfy M">MR Loutfy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wong, Ss" uniqKey="Wong S">SS Wong</name>
</author>
<author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shah, Nr" uniqKey="Shah N">NR Shah</name>
</author>
<author><name sortKey="Sunderland, A" uniqKey="Sunderland A">A Sunderland</name>
</author>
<author><name sortKey="Grdzelishvili, Vz" uniqKey="Grdzelishvili V">VZ Grdzelishvili</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pearlman, Bl" uniqKey="Pearlman B">BL Pearlman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Paeshuyse, J" uniqKey="Paeshuyse J">J Paeshuyse</name>
</author>
<author><name sortKey="Dallmeier, K" uniqKey="Dallmeier K">K Dallmeier</name>
</author>
<author><name sortKey="Neyts, J" uniqKey="Neyts J">J Neyts</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wise, J" uniqKey="Wise J">J Wise</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumaki, Y" uniqKey="Kumaki Y">Y Kumaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumaki, Y" uniqKey="Kumaki Y">Y Kumaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gowen, Bb" uniqKey="Gowen B">BB Gowen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name>
</author>
<author><name sortKey="Michaelis, M" uniqKey="Michaelis M">M Michaelis</name>
</author>
<author><name sortKey="Baer, Pc" uniqKey="Baer P">PC Baer</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
<author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saijo, M" uniqKey="Saijo M">M Saijo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumaki, Y" uniqKey="Kumaki Y">Y Kumaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sainz, Bjr" uniqKey="Sainz B">BJr Sainz</name>
</author>
<author><name sortKey="Mossel, Ec" uniqKey="Mossel E">EC Mossel</name>
</author>
<author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name>
</author>
<author><name sortKey="Garry, Rf" uniqKey="Garry R">RF Garry</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zheng, B" uniqKey="Zheng B">B Zheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Paragas, J" uniqKey="Paragas J">J Paragas</name>
</author>
<author><name sortKey="Blatt, Lm" uniqKey="Blatt L">LM Blatt</name>
</author>
<author><name sortKey="Hartmann, C" uniqKey="Hartmann C">C Hartmann</name>
</author>
<author><name sortKey="Huggins, Jw" uniqKey="Huggins J">JW Huggins</name>
</author>
<author><name sortKey="Endy, Tp" uniqKey="Endy T">TP Endy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hensley, Le" uniqKey="Hensley L">LE Hensley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stroher, U" uniqKey="Stroher U">U Stroher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koren, G" uniqKey="Koren G">G Koren</name>
</author>
<author><name sortKey="King, S" uniqKey="King S">S King</name>
</author>
<author><name sortKey="Knowles, S" uniqKey="Knowles S">S Knowles</name>
</author>
<author><name sortKey="Phillips, E" uniqKey="Phillips E">E Phillips</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, N" uniqKey="Lee N">N Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tsang, Kw" uniqKey="Tsang K">KW Tsang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tsang, K" uniqKey="Tsang K">K Tsang</name>
</author>
<author><name sortKey="Seto, Wh" uniqKey="Seto W">WH Seto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Groneberg, Da" uniqKey="Groneberg D">DA Groneberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brochot, E" uniqKey="Brochot E">E Brochot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bausch, Dg" uniqKey="Bausch D">DG Bausch</name>
</author>
<author><name sortKey="Hadi, Cm" uniqKey="Hadi C">CM Hadi</name>
</author>
<author><name sortKey="Khan, Sh" uniqKey="Khan S">SH Khan</name>
</author>
<author><name sortKey="Lertora, Jj" uniqKey="Lertora J">JJ Lertora</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, F" uniqKey="Chen F">F Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Weiss, Rc" uniqKey="Weiss R">RC Weiss</name>
</author>
<author><name sortKey="Oostrom Ram, T" uniqKey="Oostrom Ram T">T Oostrom-Ram</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wills, Rj" uniqKey="Wills R">RJ Wills</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hausfater, P" uniqKey="Hausfater P">P Hausfater</name>
</author>
<author><name sortKey="Cacoub, P" uniqKey="Cacoub P">P Cacoub</name>
</author>
<author><name sortKey="Assogba, U" uniqKey="Assogba U">U Assogba</name>
</author>
<author><name sortKey="Lebon, P" uniqKey="Lebon P">P Lebon</name>
</author>
<author><name sortKey="Piette, Jc" uniqKey="Piette J">JC Piette</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reed, Lj" uniqKey="Reed L">LJ Reed</name>
</author>
<author><name sortKey="Muench, Ha" uniqKey="Muench H">HA Muench</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id>
<journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id>
<journal-title-group><journal-title>Scientific Reports</journal-title>
</journal-title-group>
<issn pub-type="epub">2045-2322</issn>
<publisher><publisher-name>Nature Publishing Group UK</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23594967</article-id>
<article-id pub-id-type="pmc">3629412</article-id>
<article-id pub-id-type="publisher-id">BFsrep01686</article-id>
<article-id pub-id-type="doi">10.1038/srep01686</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Falzarano</surname>
<given-names>Darryl</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>de Wit</surname>
<given-names>Emmie</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Martellaro</surname>
<given-names>Cynthia</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Callison</surname>
<given-names>Julie</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Munster</surname>
<given-names>Vincent J.</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Feldmann</surname>
<given-names>Heinz</given-names>
</name>
<address><email>feldmannh@niaid.nih.gov</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<aff id="Aff1"><label>1</label>
Disease Modeling and Transmission Unit, ,</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id>
<institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id>
<institution>Virus Ecology Unit, Division of Intramural Research,</institution>
<institution>Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories,</institution>
</institution-wrap>
Hamilton, MT USA</aff>
<aff id="Aff3"><label>3</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.21613.37</institution-id>
<institution-id institution-id-type="ISNI">0000 0004 1936 9609</institution-id>
<institution>Department of Medical Microbiology,</institution>
<institution>University of Manitoba,</institution>
</institution-wrap>
Winnipeg, MB Canada</aff>
</contrib-group>
<pub-date pub-type="epub"><day>18</day>
<month>4</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>18</day>
<month>4</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="collection"><year>2013</year>
</pub-date>
<volume>3</volume>
<elocation-id>1686</elocation-id>
<history><date date-type="received"><day>6</day>
<month>3</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>27</day>
<month>3</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2013</copyright-statement>
<license license-type="OpenAccess"><license-p>This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">http://creativecommons.org/licenses/by-nc-sa/3.0/</ext-link>
</license-p>
</license>
</permissions>
<abstract id="Abs1"><p>The identification of a novel β coronavirus, nCoV, as the causative agent of severe respiratory illness in humans originating in Saudi Arabia, Qatar and Jordan has raised concerns about the possibility of a coronavirus pandemic similar to that of SARS-CoV. As a definitive treatment regimen has never been thoroughly evaluated for coronavirus infections, there is an urgent need to rapidly identify potential therapeutics to address future cases of nCoV. To determine an intervention strategy, the effect of interferon-α2b and ribavirin on nCoV isolate hCoV-EMC/2012 replication in Vero and LLC-MK2 cells was evaluated. hCoV-EMC/2012 was sensitive to both interferon-α2b and ribavirin alone in Vero and LLC-MK2 cells, but only at relatively high concentrations; however, when combined, lower concentrations of interferon-α2b and ribavirin achieved comparable endpoints. Thus, a combination of interferon-α2b and ribavirin, which are already commonly used in the clinic, may be useful for patient management in the event of future nCoV infections.</p>
</abstract>
<kwd-group kwd-group-type="npg-subject"><title>Subject terms</title>
<kwd>Antivirals</kwd>
<kwd>SARS virus</kwd>
<kwd>Antiviral agents</kwd>
<kwd>Viral infection</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2013</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Montana</li>
</region>
</list>
<tree><noCountry><name sortKey="Callison, Julie" sort="Callison, Julie" uniqKey="Callison J" first="Julie" last="Callison">Julie Callison</name>
<name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<name sortKey="Martellaro, Cynthia" sort="Martellaro, Cynthia" uniqKey="Martellaro C" first="Cynthia" last="Martellaro">Cynthia Martellaro</name>
</noCountry>
<country name="États-Unis"><region name="Montana"><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000973 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000973 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:3629412 |texte= Inhibition of novel β coronavirus replication by a combination of interferon-α2b and ribavirin }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:23594967" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |